Heterologous production of a feruloyl esterase from Pleurotus sapidus synthesizing feruloyl-saccharide esters

The feruloyl esterase (FAE) gene EST1 from the basidiomycete Pleurotus sapidus was heterologously expressed in Escherichia coli and Pichia pastoris. Catalytically active recombinant Est1 was secreted using P. pastoris as a host. For expression in P. pastoris, the expression vector pPIC9K was applied...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biotechnology and applied biochemistry 2016-11, Vol.63 (6), p.852-862
Hauptverfasser:	Kelle, Sebastian, Nieter, Annabel, Krings, Ulrich, Zelena, Katerina, Linke, Diana, Berger, Ralf G.
Format:	Artikel
Sprache:	eng
Schlagworte:	basidiomycete Basidiomycetes Carboxylic Ester Hydrolases - biosynthesis Carboxylic Ester Hydrolases - genetics Carboxylic Ester Hydrolases - isolation & purification Carboxylic Ester Hydrolases - metabolism Coumaric Acids - chemistry Escherichia coli Escherichia coli - genetics Esterases esterification Esters Esters - chemistry feruloyl esterase functional expression functional expression, Pichia pastoris Genes Genetic Engineering Genetic Vectors - genetics Hydroxybenzoates - metabolism Kinetics Maltose - biosynthesis Maltose - chemistry Mathematical models Methyl alcohol Pichia - genetics Pichia pastoris Pleurotus Pleurotus - enzymology Pleurotus - genetics Pleurotus sapidus Recombinant Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Substrate Specificity Substrates Synthesis Triticum - chemistry Triticum aestivum
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!