Matrix-assisted laser desorption/ionization imaging mass spectrometry reveals changes of phospholipid distribution in induced pluripotent stem cell colony differentiation
Induced pluripotent stem cells (iPSCs) are opening up new possibilities for medicine. Understanding the regulation of iPSC biology is important when attempting to apply these cells to disease models or therapy. Changes of lipid metabolism in iPSCs were investigated by matrix-assisted laser desorptio...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2017-02, Vol.409 (4), p.1007-1016 |
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| creator | Shimizu, Yasuo Satou, Motoyasu Hayashi, Keitaro Nakamura, Yusuke Fujimaki, Mio Horibata, Yasuhiro Ando, Hiromi Watanabe, Taiji Shiobara, Taichi Chibana, Kazuyuki Takemasa, Akihiro Sugimoto, Hiroyuki Anzai, Naohiko Ishii, Yoshiki |
| description | Induced pluripotent stem cells (iPSCs) are opening up new possibilities for medicine. Understanding the regulation of iPSC biology is important when attempting to apply these cells to disease models or therapy. Changes of lipid metabolism in iPSCs were investigated by matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry (MALDI-TOF-IMS). Analysis revealed changes of the intensity and distribution of peaks at
m
/
z
782.5 and 798.5 in iPSC colonies during spontaneous differentiation. Two phosphatidylcholines (PCs) were identified: C
44
H
81
NO
8
P, PC(36:4)[M+H]+ at
m
/
z
782.5 and C
42
H
82
NO
8
P, PC(34:1)[M+K]+ at
m
/
z
798.5. The intensity of PC(36:4) showed an inverse relation between undifferentiated and differentiated iPSC colonies. PC(34:1) displayed a diffuse distribution in undifferentiated iPSC colonies, while it showed a concentric distribution in differentiated iPSC colonies, and was localized at the border of the differentiated and undifferentiated areas or the border between undifferentiated iPSC and feeder cells. These findings suggested that the distribution of lipids changes during the growth and differentiation of iPSCs and that MALDI-TOF-IMS was useful for analyzing these changes. PC(36:4) might play a role in maintaining pluripotency, while PC(34:1) might play a role in the differentiation and spread of iPSCs.
Graphical Abstract
MALDI Imaging for phosphatidylcholine distribution changes during sponteneous differentiaton of induced pluiripotent stem cells colonies |
| doi_str_mv | 10.1007/s00216-016-0015-x |
| format | Article |
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m
/
z
782.5 and 798.5 in iPSC colonies during spontaneous differentiation. Two phosphatidylcholines (PCs) were identified: C
44
H
81
NO
8
P, PC(36:4)[M+H]+ at
m
/
z
782.5 and C
42
H
82
NO
8
P, PC(34:1)[M+K]+ at
m
/
z
798.5. The intensity of PC(36:4) showed an inverse relation between undifferentiated and differentiated iPSC colonies. PC(34:1) displayed a diffuse distribution in undifferentiated iPSC colonies, while it showed a concentric distribution in differentiated iPSC colonies, and was localized at the border of the differentiated and undifferentiated areas or the border between undifferentiated iPSC and feeder cells. These findings suggested that the distribution of lipids changes during the growth and differentiation of iPSCs and that MALDI-TOF-IMS was useful for analyzing these changes. PC(36:4) might play a role in maintaining pluripotency, while PC(34:1) might play a role in the differentiation and spread of iPSCs.
Graphical Abstract
MALDI Imaging for phosphatidylcholine distribution changes during sponteneous differentiaton of induced pluiripotent stem cells colonies</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-016-0015-x</identifier><identifier>PMID: 27815610</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analytical Chemistry ; Animals ; Antibodies ; Biochemistry ; Borders ; Cell culture ; Cell Differentiation ; Cell Line ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Chromatography, High Pressure Liquid - methods ; Chromatography, Reverse-Phase - methods ; Colonies ; Desorption ; Differentiation ; Fibroblasts ; Food Science ; Imaging ; Immunohistochemistry ; Induced Pluripotent Stem Cells - cytology ; Induced Pluripotent Stem Cells - metabolism ; Ionization ; Laboratory Medicine ; Lasers ; Lipids ; Mass spectrometry ; Medicine ; Metabolism ; Methods ; Mice ; Monitoring/Environmental Analysis ; Observations ; Phosphatase ; Phosphatidylcholines - metabolism ; Phospholipids ; Physiological aspects ; Research Paper ; Scientific imaging ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Stem cells</subject><ispartof>Analytical and bioanalytical chemistry, 2017-02, Vol.409 (4), p.1007-1016</ispartof><rights>Springer-Verlag Berlin Heidelberg 2016</rights><rights>COPYRIGHT 2017 Springer</rights><rights>Analytical and Bioanalytical Chemistry is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c580t-f32b8363afaf9c9ee1627c4e951b2d7ed59ce62ab95b2ddf0b896ae754f1c5b53</citedby><cites>FETCH-LOGICAL-c580t-f32b8363afaf9c9ee1627c4e951b2d7ed59ce62ab95b2ddf0b896ae754f1c5b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-016-0015-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-016-0015-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27815610$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shimizu, Yasuo</creatorcontrib><creatorcontrib>Satou, Motoyasu</creatorcontrib><creatorcontrib>Hayashi, Keitaro</creatorcontrib><creatorcontrib>Nakamura, Yusuke</creatorcontrib><creatorcontrib>Fujimaki, Mio</creatorcontrib><creatorcontrib>Horibata, Yasuhiro</creatorcontrib><creatorcontrib>Ando, Hiromi</creatorcontrib><creatorcontrib>Watanabe, Taiji</creatorcontrib><creatorcontrib>Shiobara, Taichi</creatorcontrib><creatorcontrib>Chibana, Kazuyuki</creatorcontrib><creatorcontrib>Takemasa, Akihiro</creatorcontrib><creatorcontrib>Sugimoto, Hiroyuki</creatorcontrib><creatorcontrib>Anzai, Naohiko</creatorcontrib><creatorcontrib>Ishii, Yoshiki</creatorcontrib><title>Matrix-assisted laser desorption/ionization imaging mass spectrometry reveals changes of phospholipid distribution in induced pluripotent stem cell colony differentiation</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Induced pluripotent stem cells (iPSCs) are opening up new possibilities for medicine. Understanding the regulation of iPSC biology is important when attempting to apply these cells to disease models or therapy. Changes of lipid metabolism in iPSCs were investigated by matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry (MALDI-TOF-IMS). Analysis revealed changes of the intensity and distribution of peaks at
m
/
z
782.5 and 798.5 in iPSC colonies during spontaneous differentiation. Two phosphatidylcholines (PCs) were identified: C
44
H
81
NO
8
P, PC(36:4)[M+H]+ at
m
/
z
782.5 and C
42
H
82
NO
8
P, PC(34:1)[M+K]+ at
m
/
z
798.5. The intensity of PC(36:4) showed an inverse relation between undifferentiated and differentiated iPSC colonies. PC(34:1) displayed a diffuse distribution in undifferentiated iPSC colonies, while it showed a concentric distribution in differentiated iPSC colonies, and was localized at the border of the differentiated and undifferentiated areas or the border between undifferentiated iPSC and feeder cells. These findings suggested that the distribution of lipids changes during the growth and differentiation of iPSCs and that MALDI-TOF-IMS was useful for analyzing these changes. PC(36:4) might play a role in maintaining pluripotency, while PC(34:1) might play a role in the differentiation and spread of iPSCs.
Graphical Abstract
MALDI Imaging for phosphatidylcholine distribution changes during sponteneous differentiaton of induced pluiripotent stem cells colonies</description><subject>Analytical Chemistry</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Biochemistry</subject><subject>Borders</subject><subject>Cell culture</subject><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Chromatography, Reverse-Phase - methods</subject><subject>Colonies</subject><subject>Desorption</subject><subject>Differentiation</subject><subject>Fibroblasts</subject><subject>Food Science</subject><subject>Imaging</subject><subject>Immunohistochemistry</subject><subject>Induced Pluripotent Stem Cells - cytology</subject><subject>Induced Pluripotent Stem Cells - metabolism</subject><subject>Ionization</subject><subject>Laboratory Medicine</subject><subject>Lasers</subject><subject>Lipids</subject><subject>Mass spectrometry</subject><subject>Medicine</subject><subject>Metabolism</subject><subject>Methods</subject><subject>Mice</subject><subject>Monitoring/Environmental Analysis</subject><subject>Observations</subject><subject>Phosphatase</subject><subject>Phosphatidylcholines - metabolism</subject><subject>Phospholipids</subject><subject>Physiological aspects</subject><subject>Research Paper</subject><subject>Scientific imaging</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><subject>Stem 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laser desorption/ionization imaging mass spectrometry reveals changes of phospholipid distribution in induced pluripotent stem cell colony differentiation</title><author>Shimizu, Yasuo ; Satou, Motoyasu ; Hayashi, Keitaro ; Nakamura, Yusuke ; Fujimaki, Mio ; Horibata, Yasuhiro ; Ando, Hiromi ; Watanabe, Taiji ; Shiobara, Taichi ; Chibana, Kazuyuki ; Takemasa, Akihiro ; Sugimoto, Hiroyuki ; Anzai, Naohiko ; Ishii, Yoshiki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c580t-f32b8363afaf9c9ee1627c4e951b2d7ed59ce62ab95b2ddf0b896ae754f1c5b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Analytical Chemistry</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Biochemistry</topic><topic>Borders</topic><topic>Cell culture</topic><topic>Cell Differentiation</topic><topic>Cell Line</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Chromatography, Reverse-Phase - methods</topic><topic>Colonies</topic><topic>Desorption</topic><topic>Differentiation</topic><topic>Fibroblasts</topic><topic>Food Science</topic><topic>Imaging</topic><topic>Immunohistochemistry</topic><topic>Induced Pluripotent Stem Cells - cytology</topic><topic>Induced Pluripotent Stem Cells - metabolism</topic><topic>Ionization</topic><topic>Laboratory Medicine</topic><topic>Lasers</topic><topic>Lipids</topic><topic>Mass spectrometry</topic><topic>Medicine</topic><topic>Metabolism</topic><topic>Methods</topic><topic>Mice</topic><topic>Monitoring/Environmental Analysis</topic><topic>Observations</topic><topic>Phosphatase</topic><topic>Phosphatidylcholines - metabolism</topic><topic>Phospholipids</topic><topic>Physiological aspects</topic><topic>Research Paper</topic><topic>Scientific 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Mio</au><au>Horibata, Yasuhiro</au><au>Ando, Hiromi</au><au>Watanabe, Taiji</au><au>Shiobara, Taichi</au><au>Chibana, Kazuyuki</au><au>Takemasa, Akihiro</au><au>Sugimoto, Hiroyuki</au><au>Anzai, Naohiko</au><au>Ishii, Yoshiki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Matrix-assisted laser desorption/ionization imaging mass spectrometry reveals changes of phospholipid distribution in induced pluripotent stem cell colony differentiation</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2017-02-01</date><risdate>2017</risdate><volume>409</volume><issue>4</issue><spage>1007</spage><epage>1016</epage><pages>1007-1016</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Induced pluripotent stem cells (iPSCs) are opening up new possibilities for medicine. Understanding the regulation of iPSC biology is important when attempting to apply these cells to disease models or therapy. Changes of lipid metabolism in iPSCs were investigated by matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry (MALDI-TOF-IMS). Analysis revealed changes of the intensity and distribution of peaks at
m
/
z
782.5 and 798.5 in iPSC colonies during spontaneous differentiation. Two phosphatidylcholines (PCs) were identified: C
44
H
81
NO
8
P, PC(36:4)[M+H]+ at
m
/
z
782.5 and C
42
H
82
NO
8
P, PC(34:1)[M+K]+ at
m
/
z
798.5. The intensity of PC(36:4) showed an inverse relation between undifferentiated and differentiated iPSC colonies. PC(34:1) displayed a diffuse distribution in undifferentiated iPSC colonies, while it showed a concentric distribution in differentiated iPSC colonies, and was localized at the border of the differentiated and undifferentiated areas or the border between undifferentiated iPSC and feeder cells. These findings suggested that the distribution of lipids changes during the growth and differentiation of iPSCs and that MALDI-TOF-IMS was useful for analyzing these changes. PC(36:4) might play a role in maintaining pluripotency, while PC(34:1) might play a role in the differentiation and spread of iPSCs.
Graphical Abstract
MALDI Imaging for phosphatidylcholine distribution changes during sponteneous differentiaton of induced pluiripotent stem cells colonies</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>27815610</pmid><doi>10.1007/s00216-016-0015-x</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1618-2642 |
| ispartof | Analytical and bioanalytical chemistry, 2017-02, Vol.409 (4), p.1007-1016 |
| issn | 1618-2642 1618-2650 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1879999433 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Analytical Chemistry Animals Antibodies Biochemistry Borders Cell culture Cell Differentiation Cell Line Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Chromatography, High Pressure Liquid - methods Chromatography, Reverse-Phase - methods Colonies Desorption Differentiation Fibroblasts Food Science Imaging Immunohistochemistry Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - metabolism Ionization Laboratory Medicine Lasers Lipids Mass spectrometry Medicine Metabolism Methods Mice Monitoring/Environmental Analysis Observations Phosphatase Phosphatidylcholines - metabolism Phospholipids Physiological aspects Research Paper Scientific imaging Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Stem cells |
| title | Matrix-assisted laser desorption/ionization imaging mass spectrometry reveals changes of phospholipid distribution in induced pluripotent stem cell colony differentiation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T17%3A46%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Matrix-assisted%20laser%20desorption/ionization%20imaging%20mass%20spectrometry%20reveals%20changes%20of%20phospholipid%20distribution%20in%20induced%20pluripotent%20stem%20cell%20colony%20differentiation&rft.jtitle=Analytical%20and%20bioanalytical%20chemistry&rft.au=Shimizu,%20Yasuo&rft.date=2017-02-01&rft.volume=409&rft.issue=4&rft.spage=1007&rft.epage=1016&rft.pages=1007-1016&rft.issn=1618-2642&rft.eissn=1618-2650&rft_id=info:doi/10.1007/s00216-016-0015-x&rft_dat=%3Cgale_proqu%3EA550916094%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1865253711&rft_id=info:pmid/27815610&rft_galeid=A550916094&rfr_iscdi=true |