Evaluation of Staphylococcus aureus DNA aptamer by enzyme‐linked aptamer assay and isothermal titration calorimetry

To monitor the specificity of Staphylococcus aureus aptamer (SA‐31) against its target cell, we used enzyme‐linked aptamer assay. In the presence of target cell, horseradish peroxidase–conjugated streptavidin bound to biotin‐labeled SA‐31 showed specific binding to S aureus among 3 different bacter...

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Veröffentlicht in:	Journal of molecular recognition 2017-02, Vol.30 (2), p.np-n/a
Hauptverfasser:	Bayrac, Ceren, Oktem, Huseyin Avni
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Aptamers Aptamers, Nucleotide - chemistry Aptamers, Nucleotide - metabolism Assaying Bacteria Bacterial Proteins - chemistry Binding Biotin Calorimetry - methods Deoxyribonucleic acid DNA DNA aptamer Enzymes enzyme‐linked aptamer assay Exothermic reactions Heat measurement Horseradish Peroxidase - chemistry Ion concentration isothermal titration calorimetry Kinetics Models, Molecular Nucleic Acid Conformation Peroxidase Proteins Recognition Staphylococcus aureus Staphylococcus aureus - genetics Staphylococcus aureus - growth & development S aureus Titration calorimetry
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