Identification of key genes involved in polysaccharide bioflocculant synthesis in Bacillus licheniformis
ABSTRACT The present study reports the sequenced genome of Bacillus licheniformis CGMCC 2876, which is composed of a 4,284,461 bp chromosome that contains 4,188 protein‐coding genes, 72 tRNA genes, and 21 rRNA genes. Additional analysis revealed an eps gene cluster with 16 open reading frames. Conse...
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| Veröffentlicht in: | Biotechnology and bioengineering 2017-03, Vol.114 (3), p.645-655 |
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| creator | Chen, Zhen Liu, Peize Li, Zhipeng Yu, Wencheng Wang, Zhi Yao, Haosheng Wang, Yuanpeng Li, Qingbiao Deng, Xu He, Ning |
| description | ABSTRACT
The present study reports the sequenced genome of Bacillus licheniformis CGMCC 2876, which is composed of a 4,284,461 bp chromosome that contains 4,188 protein‐coding genes, 72 tRNA genes, and 21 rRNA genes. Additional analysis revealed an eps gene cluster with 16 open reading frames. Conserved Domains Database analysis combined with qPCR experiments indicated that all genes in this cluster were involved in polysaccharide bioflocculant synthesis. Phosphoglucomutase and UDP‐glucose pyrophosphorylase were supposed to be key enzymes in polysaccharide secretion in B. licheniformis. A biosynthesis pathway for the production of polysaccharide bioflocculant involving the integration of individual genes was proposed based on functional analysis. Overexpression of epsDEF from the eps gene cluster in B. licheniformis CGMCC 2876 increased the flocculating activity of the recombinant strain by 90% compared to the original strain. Similarly, the crude yield of polysaccharide bioflocculant was enhanced by 27.8%. Overexpression of the UDP‐glucose pyrophosphorylase gene not only increased the flocculating activity by 71% but also increased bioflocculant yield by 13.3%. Independent of UDP‐N‐acetyl‐D‐mannosamine dehydrogenase gene, flocculating activity, and polysaccharide yield were negatively impacted by overexpression of the UDP‐N‐acetylglucosamine 2‐epimerase gene. Overall, epsDEF and gtaB2 were identified as key genes for polysaccharide bioflocculant synthesis in B. licheniformis. These results will be useful for further engineering of B. licheniformis for industrial bioflocculant production. Biotechnol. Bioeng. 2017;114: 645–655. © 2016 Wiley Periodicals, Inc.
This paper identified key genes involved in polysaccharide bioflocculant production in Bacillus licheniformis CGMCC2876 and proposed a pathway for this production. The identification of the genetic information will provide new tools to engineer B.licheniformis for bioflocculant industrial production. |
| doi_str_mv | 10.1002/bit.26189 |
| format | Article |
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The present study reports the sequenced genome of Bacillus licheniformis CGMCC 2876, which is composed of a 4,284,461 bp chromosome that contains 4,188 protein‐coding genes, 72 tRNA genes, and 21 rRNA genes. Additional analysis revealed an eps gene cluster with 16 open reading frames. Conserved Domains Database analysis combined with qPCR experiments indicated that all genes in this cluster were involved in polysaccharide bioflocculant synthesis. Phosphoglucomutase and UDP‐glucose pyrophosphorylase were supposed to be key enzymes in polysaccharide secretion in B. licheniformis. A biosynthesis pathway for the production of polysaccharide bioflocculant involving the integration of individual genes was proposed based on functional analysis. Overexpression of epsDEF from the eps gene cluster in B. licheniformis CGMCC 2876 increased the flocculating activity of the recombinant strain by 90% compared to the original strain. Similarly, the crude yield of polysaccharide bioflocculant was enhanced by 27.8%. Overexpression of the UDP‐glucose pyrophosphorylase gene not only increased the flocculating activity by 71% but also increased bioflocculant yield by 13.3%. Independent of UDP‐N‐acetyl‐D‐mannosamine dehydrogenase gene, flocculating activity, and polysaccharide yield were negatively impacted by overexpression of the UDP‐N‐acetylglucosamine 2‐epimerase gene. Overall, epsDEF and gtaB2 were identified as key genes for polysaccharide bioflocculant synthesis in B. licheniformis. These results will be useful for further engineering of B. licheniformis for industrial bioflocculant production. Biotechnol. Bioeng. 2017;114: 645–655. © 2016 Wiley Periodicals, Inc.
This paper identified key genes involved in polysaccharide bioflocculant production in Bacillus licheniformis CGMCC2876 and proposed a pathway for this production. The identification of the genetic information will provide new tools to engineer B.licheniformis for bioflocculant industrial production.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.26189</identifier><identifier>PMID: 27667128</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Bacillus licheniformis ; Bacillus licheniformis - enzymology ; Bacillus licheniformis - genetics ; Bacillus licheniformis - metabolism ; Bioengineering ; Biosynthesis ; Clusters ; comparative genomics ; Epimerase ; Eps gene ; Flocculating ; Flocculation ; Functional analysis ; Genes ; Genetics ; genome sequence ; Genome, Bacterial - genetics ; Genomes ; Glucose ; metabolic engineering ; Metabolic Engineering - methods ; N-Acetylglucosamine ; Open reading frames ; Pathways ; Phosphoglucomutase ; polysaccharide bioflocculant ; Polysaccharides ; Polysaccharides, Bacterial - analysis ; Polysaccharides, Bacterial - genetics ; Polysaccharides, Bacterial - metabolism ; Proteins ; rRNA ; Secretion ; Synthesis ; tRNA ; Yield</subject><ispartof>Biotechnology and bioengineering, 2017-03, Vol.114 (3), p.645-655</ispartof><rights>2016 Wiley Periodicals, Inc.</rights><rights>2017 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4849-6692853c926b40d062d301719c4c88134c42e4c20d9f23eeb8a24393c5ceeb2f3</citedby><cites>FETCH-LOGICAL-c4849-6692853c926b40d062d301719c4c88134c42e4c20d9f23eeb8a24393c5ceeb2f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.26189$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.26189$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27667128$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Zhen</creatorcontrib><creatorcontrib>Liu, Peize</creatorcontrib><creatorcontrib>Li, Zhipeng</creatorcontrib><creatorcontrib>Yu, Wencheng</creatorcontrib><creatorcontrib>Wang, Zhi</creatorcontrib><creatorcontrib>Yao, Haosheng</creatorcontrib><creatorcontrib>Wang, Yuanpeng</creatorcontrib><creatorcontrib>Li, Qingbiao</creatorcontrib><creatorcontrib>Deng, Xu</creatorcontrib><creatorcontrib>He, Ning</creatorcontrib><title>Identification of key genes involved in polysaccharide bioflocculant synthesis in Bacillus licheniformis</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol Bioeng</addtitle><description>ABSTRACT
The present study reports the sequenced genome of Bacillus licheniformis CGMCC 2876, which is composed of a 4,284,461 bp chromosome that contains 4,188 protein‐coding genes, 72 tRNA genes, and 21 rRNA genes. Additional analysis revealed an eps gene cluster with 16 open reading frames. Conserved Domains Database analysis combined with qPCR experiments indicated that all genes in this cluster were involved in polysaccharide bioflocculant synthesis. Phosphoglucomutase and UDP‐glucose pyrophosphorylase were supposed to be key enzymes in polysaccharide secretion in B. licheniformis. A biosynthesis pathway for the production of polysaccharide bioflocculant involving the integration of individual genes was proposed based on functional analysis. Overexpression of epsDEF from the eps gene cluster in B. licheniformis CGMCC 2876 increased the flocculating activity of the recombinant strain by 90% compared to the original strain. Similarly, the crude yield of polysaccharide bioflocculant was enhanced by 27.8%. Overexpression of the UDP‐glucose pyrophosphorylase gene not only increased the flocculating activity by 71% but also increased bioflocculant yield by 13.3%. Independent of UDP‐N‐acetyl‐D‐mannosamine dehydrogenase gene, flocculating activity, and polysaccharide yield were negatively impacted by overexpression of the UDP‐N‐acetylglucosamine 2‐epimerase gene. Overall, epsDEF and gtaB2 were identified as key genes for polysaccharide bioflocculant synthesis in B. licheniformis. These results will be useful for further engineering of B. licheniformis for industrial bioflocculant production. Biotechnol. Bioeng. 2017;114: 645–655. © 2016 Wiley Periodicals, Inc.
This paper identified key genes involved in polysaccharide bioflocculant production in Bacillus licheniformis CGMCC2876 and proposed a pathway for this production. The identification of the genetic information will provide new tools to engineer B.licheniformis for bioflocculant industrial production.</description><subject>Bacillus licheniformis</subject><subject>Bacillus licheniformis - enzymology</subject><subject>Bacillus licheniformis - genetics</subject><subject>Bacillus licheniformis - metabolism</subject><subject>Bioengineering</subject><subject>Biosynthesis</subject><subject>Clusters</subject><subject>comparative genomics</subject><subject>Epimerase</subject><subject>Eps gene</subject><subject>Flocculating</subject><subject>Flocculation</subject><subject>Functional analysis</subject><subject>Genes</subject><subject>Genetics</subject><subject>genome sequence</subject><subject>Genome, Bacterial - genetics</subject><subject>Genomes</subject><subject>Glucose</subject><subject>metabolic engineering</subject><subject>Metabolic Engineering - methods</subject><subject>N-Acetylglucosamine</subject><subject>Open reading frames</subject><subject>Pathways</subject><subject>Phosphoglucomutase</subject><subject>polysaccharide bioflocculant</subject><subject>Polysaccharides</subject><subject>Polysaccharides, Bacterial - analysis</subject><subject>Polysaccharides, Bacterial - genetics</subject><subject>Polysaccharides, Bacterial - metabolism</subject><subject>Proteins</subject><subject>rRNA</subject><subject>Secretion</subject><subject>Synthesis</subject><subject>tRNA</subject><subject>Yield</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFu1DAQhi1ERZfCgRdAkbjQQ9rx2HHsI60orFSpl3K2EmfCunjjJU6K8vZ4u4UDElXlgz3S529m9DP2jsMZB8Dz1k9nqLg2L9iKg6lLQAMv2QoAVCkqg8fsdUp3uay1Uq_YMdZK1Rz1im3WHQ2T771rJh-HIvbFD1qK7zRQKvxwH8M9dflR7GJYUuPcphl9R0XrYx-ic3NohqlIyzBtKPn9l-KicT6EORXBuw0Nvo_j1qc37KhvQqK3j_cJ-3b1-fbya3l982V9-em6dFJLUyplUFfCGVSthA4UdgJ4zY2TTmsupJNI0iF0pkdB1OoGpTDCVS4X2IsT9vHg3Y3x50xpsrm5o5DnpDgny3VtjOGA_Bmo0gKkEfgMVFT5CFll9MM_6F2cxyHvbBGquoKs1E9RuS3UXGjcU6cHyo0xpZF6uxv9thkXy8Huo7c5evsQfWbfPxrndkvdX_JP1hk4PwC_fKDl_yZ7sb49KH8DAXe2bg</recordid><startdate>201703</startdate><enddate>201703</enddate><creator>Chen, Zhen</creator><creator>Liu, Peize</creator><creator>Li, Zhipeng</creator><creator>Yu, Wencheng</creator><creator>Wang, Zhi</creator><creator>Yao, Haosheng</creator><creator>Wang, Yuanpeng</creator><creator>Li, Qingbiao</creator><creator>Deng, Xu</creator><creator>He, Ning</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201703</creationdate><title>Identification of key genes involved in polysaccharide bioflocculant synthesis in Bacillus licheniformis</title><author>Chen, Zhen ; 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The present study reports the sequenced genome of Bacillus licheniformis CGMCC 2876, which is composed of a 4,284,461 bp chromosome that contains 4,188 protein‐coding genes, 72 tRNA genes, and 21 rRNA genes. Additional analysis revealed an eps gene cluster with 16 open reading frames. Conserved Domains Database analysis combined with qPCR experiments indicated that all genes in this cluster were involved in polysaccharide bioflocculant synthesis. Phosphoglucomutase and UDP‐glucose pyrophosphorylase were supposed to be key enzymes in polysaccharide secretion in B. licheniformis. A biosynthesis pathway for the production of polysaccharide bioflocculant involving the integration of individual genes was proposed based on functional analysis. Overexpression of epsDEF from the eps gene cluster in B. licheniformis CGMCC 2876 increased the flocculating activity of the recombinant strain by 90% compared to the original strain. Similarly, the crude yield of polysaccharide bioflocculant was enhanced by 27.8%. Overexpression of the UDP‐glucose pyrophosphorylase gene not only increased the flocculating activity by 71% but also increased bioflocculant yield by 13.3%. Independent of UDP‐N‐acetyl‐D‐mannosamine dehydrogenase gene, flocculating activity, and polysaccharide yield were negatively impacted by overexpression of the UDP‐N‐acetylglucosamine 2‐epimerase gene. Overall, epsDEF and gtaB2 were identified as key genes for polysaccharide bioflocculant synthesis in B. licheniformis. These results will be useful for further engineering of B. licheniformis for industrial bioflocculant production. Biotechnol. Bioeng. 2017;114: 645–655. © 2016 Wiley Periodicals, Inc.
This paper identified key genes involved in polysaccharide bioflocculant production in Bacillus licheniformis CGMCC2876 and proposed a pathway for this production. The identification of the genetic information will provide new tools to engineer B.licheniformis for bioflocculant industrial production.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>27667128</pmid><doi>10.1002/bit.26189</doi><tpages>11</tpages></addata></record> |
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| subjects | Bacillus licheniformis Bacillus licheniformis - enzymology Bacillus licheniformis - genetics Bacillus licheniformis - metabolism Bioengineering Biosynthesis Clusters comparative genomics Epimerase Eps gene Flocculating Flocculation Functional analysis Genes Genetics genome sequence Genome, Bacterial - genetics Genomes Glucose metabolic engineering Metabolic Engineering - methods N-Acetylglucosamine Open reading frames Pathways Phosphoglucomutase polysaccharide bioflocculant Polysaccharides Polysaccharides, Bacterial - analysis Polysaccharides, Bacterial - genetics Polysaccharides, Bacterial - metabolism Proteins rRNA Secretion Synthesis tRNA Yield |
| title | Identification of key genes involved in polysaccharide bioflocculant synthesis in Bacillus licheniformis |
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