In Vitro Evaluation of Effects of Mg-6Zn Alloy Extracts on Apoptosis of Intestinal Epithelial Cells
We assessed the in vitro cytotoxicity of Mg-6Zn alloy and analyzed the cell apoptosis rate and the expression of caspase-3 to evaluate the effects of Mg-6Zn alloy extracts on apoptosis of intestinal epithelial cells（IEC）-6. IEC-6 cells were cultured in different concentrations of Mg-6Zn alloy extrac...
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| Veröffentlicht in: | Journal of Wuhan University of Technology. Materials science edition 2016-12, Vol.31 (6), p.1387-1393 |
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| container_title | Journal of Wuhan University of Technology. Materials science edition |
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| creator | Wang, Xiaohu Chen, Yigang Yu, Song Wang, Zhigang Zhang, Xiaonong Zhao, Changli Zhang, Shaoxiang Yan, Jun Gu, Baojun |
| description | We assessed the in vitro cytotoxicity of Mg-6Zn alloy and analyzed the cell apoptosis rate and the expression of caspase-3 to evaluate the effects of Mg-6Zn alloy extracts on apoptosis of intestinal epithelial cells（IEC）-6. IEC-6 cells were cultured in different concentrations of Mg-6Zn alloy extracts（40%, 20%） and in the control group. The indirect effects of Mg-6Zn alloy on IEC-6 cells were studied by calculating the cell relative growth rate（RGR）, measuring the apoptosis of IEC-6 cells through flow cytometry, and investigating the expression of caspase-3 using real-time polymerase chain reaction. The experimental results show that the cytotoxicity of these extracts is Grade 0-1. The level of apoptosis in IEC-6 cells cultured in 40% Mg-6Zn alloy extracts is significantly higher than that in cells treated with 20% extract and the control group. The expression of caspase-3 is found to be up-regulated in the 40% extract as compared to 20% extract and the control group. Taken together, the data show that the Mg-6Zn alloy in 40% and 20% concentration extracts proves noncytotoxicity. But the 40% concentration of Mg-6Zn alloy extract can induce the apoptosis and the related caspase-3 expression in vitro. |
| doi_str_mv | 10.1007/s11595-016-1543-0 |
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IEC-6 cells were cultured in different concentrations of Mg-6Zn alloy extracts（40%, 20%） and in the control group. The indirect effects of Mg-6Zn alloy on IEC-6 cells were studied by calculating the cell relative growth rate（RGR）, measuring the apoptosis of IEC-6 cells through flow cytometry, and investigating the expression of caspase-3 using real-time polymerase chain reaction. The experimental results show that the cytotoxicity of these extracts is Grade 0-1. The level of apoptosis in IEC-6 cells cultured in 40% Mg-6Zn alloy extracts is significantly higher than that in cells treated with 20% extract and the control group. The expression of caspase-3 is found to be up-regulated in the 40% extract as compared to 20% extract and the control group. Taken together, the data show that the Mg-6Zn alloy in 40% and 20% concentration extracts proves noncytotoxicity. But the 40% concentration of Mg-6Zn alloy extract can induce the apoptosis and the related caspase-3 expression in vitro.</description><identifier>ISSN: 1000-2413</identifier><identifier>EISSN: 1993-0437</identifier><identifier>DOI: 10.1007/s11595-016-1543-0</identifier><language>eng</language><publisher>Wuhan: Wuhan University of Technology</publisher><subject>Apoptosis ; Biomaterials ; caspase-3 ; Chemistry and Materials Science ; Flow cytometry ; IEC-6细胞 ; In vitro testing ; Magnesium base alloys ; Materials Science ; Mathematical analysis ; Quality ; Real time ; Toxicity ; 合金 ; 实验 ; 浸提液 ; 细胞凋亡 ; 肠上皮</subject><ispartof>Journal of Wuhan University of Technology. Materials science edition, 2016-12, Vol.31 (6), p.1387-1393</ispartof><rights>Wuhan University of Technology and Springer-Verlag Berlin Heidelberg 2016</rights><rights>Journal of Wuhan University of Technology-Mater. Sci. Ed. is a copyright of Springer, 2016.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c328t-2b6e164227ede8a83e001d93620f88250e9ca81a4f3d9376933ee830f75776f13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84253X/84253X.jpg</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11595-016-1543-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11595-016-1543-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Wang, Xiaohu</creatorcontrib><creatorcontrib>Chen, Yigang</creatorcontrib><creatorcontrib>Yu, Song</creatorcontrib><creatorcontrib>Wang, Zhigang</creatorcontrib><creatorcontrib>Zhang, Xiaonong</creatorcontrib><creatorcontrib>Zhao, Changli</creatorcontrib><creatorcontrib>Zhang, Shaoxiang</creatorcontrib><creatorcontrib>Yan, Jun</creatorcontrib><creatorcontrib>Gu, Baojun</creatorcontrib><title>In Vitro Evaluation of Effects of Mg-6Zn Alloy Extracts on Apoptosis of Intestinal Epithelial Cells</title><title>Journal of Wuhan University of Technology. Materials science edition</title><addtitle>J. Wuhan Univ. Technol.-Mat. Sci. Edit</addtitle><addtitle>Journal of Wuhan University of Technology. Materials Science Edition</addtitle><description>We assessed the in vitro cytotoxicity of Mg-6Zn alloy and analyzed the cell apoptosis rate and the expression of caspase-3 to evaluate the effects of Mg-6Zn alloy extracts on apoptosis of intestinal epithelial cells（IEC）-6. IEC-6 cells were cultured in different concentrations of Mg-6Zn alloy extracts（40%, 20%） and in the control group. The indirect effects of Mg-6Zn alloy on IEC-6 cells were studied by calculating the cell relative growth rate（RGR）, measuring the apoptosis of IEC-6 cells through flow cytometry, and investigating the expression of caspase-3 using real-time polymerase chain reaction. The experimental results show that the cytotoxicity of these extracts is Grade 0-1. The level of apoptosis in IEC-6 cells cultured in 40% Mg-6Zn alloy extracts is significantly higher than that in cells treated with 20% extract and the control group. The expression of caspase-3 is found to be up-regulated in the 40% extract as compared to 20% extract and the control group. Taken together, the data show that the Mg-6Zn alloy in 40% and 20% concentration extracts proves noncytotoxicity. But the 40% concentration of Mg-6Zn alloy extract can induce the apoptosis and the related caspase-3 expression in vitro.</description><subject>Apoptosis</subject><subject>Biomaterials</subject><subject>caspase-3</subject><subject>Chemistry and Materials Science</subject><subject>Flow cytometry</subject><subject>IEC-6细胞</subject><subject>In vitro testing</subject><subject>Magnesium base alloys</subject><subject>Materials Science</subject><subject>Mathematical analysis</subject><subject>Quality</subject><subject>Real time</subject><subject>Toxicity</subject><subject>合金</subject><subject>实验</subject><subject>浸提液</subject><subject>细胞凋亡</subject><subject>肠上皮</subject><issn>1000-2413</issn><issn>1993-0437</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp9kL1OwzAUhS0EEqXwAGwRLCyB6zjxz1hVASoVsQADi2VSu02VxqntIvr2OG2FEAOTj66_c-_RQegSwy0GYHce40IUKWCa4iInKRyhARYiipyw46gBIM1yTE7RmfdLgBwIpQNUTdrkrQ7OJuWnajYq1LZNrElKY3QVfC-f5il9b5NR09htUn4Fp3YfcdLZLlhf76hJG7QPdauapOzqsNBNHeVYN40_RydGNV5fHN4her0vX8aP6fT5YTIeTdOKZDyk2QfVmOZZxvRMc8WJBsAzQWgGhvOsAC0qxbHKDYlTRgUhWnMChhWMUYPJEN3s93bOrjcxjVzVvooJVKvtxkvMmRC86FsYous_6NJuXAzfUwVQUeSMRArvqcpZ7502snP1SrmtxCD72uW-dhlrl33tEqIn23t8ZNu5dr82_2O6Ohxa2Ha-jr6fS5QBFwIEJ98cn45i</recordid><startdate>20161201</startdate><enddate>20161201</enddate><creator>Wang, Xiaohu</creator><creator>Chen, Yigang</creator><creator>Yu, Song</creator><creator>Wang, Zhigang</creator><creator>Zhang, Xiaonong</creator><creator>Zhao, Changli</creator><creator>Zhang, Shaoxiang</creator><creator>Yan, Jun</creator><creator>Gu, Baojun</creator><general>Wuhan University of Technology</general><general>Springer Nature B.V</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W92</scope><scope>~WA</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8AO</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7QF</scope></search><sort><creationdate>20161201</creationdate><title>In Vitro Evaluation of Effects of Mg-6Zn Alloy Extracts on Apoptosis of Intestinal Epithelial Cells</title><author>Wang, Xiaohu ; Chen, Yigang ; Yu, Song ; Wang, Zhigang ; Zhang, Xiaonong ; Zhao, Changli ; Zhang, Shaoxiang ; Yan, Jun ; Gu, Baojun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c328t-2b6e164227ede8a83e001d93620f88250e9ca81a4f3d9376933ee830f75776f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Apoptosis</topic><topic>Biomaterials</topic><topic>caspase-3</topic><topic>Chemistry and Materials Science</topic><topic>Flow cytometry</topic><topic>IEC-6细胞</topic><topic>In vitro testing</topic><topic>Magnesium base alloys</topic><topic>Materials Science</topic><topic>Mathematical analysis</topic><topic>Quality</topic><topic>Real time</topic><topic>Toxicity</topic><topic>合金</topic><topic>实验</topic><topic>浸提液</topic><topic>细胞凋亡</topic><topic>肠上皮</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Xiaohu</creatorcontrib><creatorcontrib>Chen, Yigang</creatorcontrib><creatorcontrib>Yu, Song</creatorcontrib><creatorcontrib>Wang, Zhigang</creatorcontrib><creatorcontrib>Zhang, Xiaonong</creatorcontrib><creatorcontrib>Zhao, Changli</creatorcontrib><creatorcontrib>Zhang, Shaoxiang</creatorcontrib><creatorcontrib>Yan, Jun</creatorcontrib><creatorcontrib>Gu, Baojun</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-工程技术</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>ProQuest Pharma Collection</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Aluminium Industry Abstracts</collection><jtitle>Journal of Wuhan University of Technology. Materials science edition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Xiaohu</au><au>Chen, Yigang</au><au>Yu, Song</au><au>Wang, Zhigang</au><au>Zhang, Xiaonong</au><au>Zhao, Changli</au><au>Zhang, Shaoxiang</au><au>Yan, Jun</au><au>Gu, Baojun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In Vitro Evaluation of Effects of Mg-6Zn Alloy Extracts on Apoptosis of Intestinal Epithelial Cells</atitle><jtitle>Journal of Wuhan University of Technology. Materials science edition</jtitle><stitle>J. Wuhan Univ. Technol.-Mat. Sci. Edit</stitle><addtitle>Journal of Wuhan University of Technology. Materials Science Edition</addtitle><date>2016-12-01</date><risdate>2016</risdate><volume>31</volume><issue>6</issue><spage>1387</spage><epage>1393</epage><pages>1387-1393</pages><issn>1000-2413</issn><eissn>1993-0437</eissn><abstract>We assessed the in vitro cytotoxicity of Mg-6Zn alloy and analyzed the cell apoptosis rate and the expression of caspase-3 to evaluate the effects of Mg-6Zn alloy extracts on apoptosis of intestinal epithelial cells（IEC）-6. IEC-6 cells were cultured in different concentrations of Mg-6Zn alloy extracts（40%, 20%） and in the control group. The indirect effects of Mg-6Zn alloy on IEC-6 cells were studied by calculating the cell relative growth rate（RGR）, measuring the apoptosis of IEC-6 cells through flow cytometry, and investigating the expression of caspase-3 using real-time polymerase chain reaction. The experimental results show that the cytotoxicity of these extracts is Grade 0-1. The level of apoptosis in IEC-6 cells cultured in 40% Mg-6Zn alloy extracts is significantly higher than that in cells treated with 20% extract and the control group. The expression of caspase-3 is found to be up-regulated in the 40% extract as compared to 20% extract and the control group. Taken together, the data show that the Mg-6Zn alloy in 40% and 20% concentration extracts proves noncytotoxicity. But the 40% concentration of Mg-6Zn alloy extract can induce the apoptosis and the related caspase-3 expression in vitro.</abstract><cop>Wuhan</cop><pub>Wuhan University of Technology</pub><doi>10.1007/s11595-016-1543-0</doi><tpages>7</tpages></addata></record> |
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| subjects | Apoptosis Biomaterials caspase-3 Chemistry and Materials Science Flow cytometry IEC-6细胞 In vitro testing Magnesium base alloys Materials Science Mathematical analysis Quality Real time Toxicity 合金 实验 浸提液 细胞凋亡 肠上皮 |
| title | In Vitro Evaluation of Effects of Mg-6Zn Alloy Extracts on Apoptosis of Intestinal Epithelial Cells |
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