Activation of Cytotoxic Natural Killer Cells after Aneurysmal Subarachnoid Haemorrhage
Abstract Objective Cell-mediated inflammation is critical in development of cerebrovascular complications after aneurysmal subarachnoid haemorrhage (aSAH). We analysed the course for activated CD16bright CD56dim cytotoxic NKCSF cells (NK) in cerebrospinal fluid (CSF) of 15 patients. Methods Patients...
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| Veröffentlicht in: | World neurosurgery 2017-05, Vol.101, p.666-676.e1 |
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| creator | Spitzer, Daniel, Dr. med Johanna Spitzer, Nina Deininger, Monika Rainer Wirtz, Christian, Prof. Dr. med König, Ralph, Prof. Dr. med Burster, Timo, PD Dr. rer. nat Kapapa, Thomas, PD Dr. med |
| description | Abstract Objective Cell-mediated inflammation is critical in development of cerebrovascular complications after aneurysmal subarachnoid haemorrhage (aSAH). We analysed the course for activated CD16bright CD56dim cytotoxic NKCSF cells (NK) in cerebrospinal fluid (CSF) of 15 patients. Methods Patients were classified by occurrence of cerebral vasospasm and delayed cerebral ischemia. NK were monitored by flow cytometry between day 1 and 14 after haemorrhage. Results 12 (80%) patients developed CV with a mean day of detection at 3.9±1.6. In those cell count for NK increased from 1.40±1.42 cells/μl on day 1 to a peak of 11.66±11.56 cells/μl on day 6.1±2.9 (p=0.001). An increase of mean CBFV in TCD from 71.33±12.93 cm/sec. to 166.20±20.19 cm/sec. (p |
| doi_str_mv | 10.1016/j.wneu.2017.03.026 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1879662134</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1878875017303388</els_id><sourcerecordid>1879662134</sourcerecordid><originalsourceid>FETCH-LOGICAL-c411t-26ac4b20f470c0ce49f9168d3791e4b5e8e6dd0d0b411ab1c4fd587f07d42c8e3</originalsourceid><addsrcrecordid>eNp9kU9v1DAQxS1ERau2X4ADypHLpuM_GzsSQlqtoEVUcChwtRx7Qr0kcbGdlv32dbSlBw744pH83hvPbwh5TaGmQJuLXf0w4VwzoLIGXgNrXpATqqRaKdm0L5_rNRyT85R2UA6nQkn-ihwzxRkvghPyY2OzvzfZh6kKfbXd55DDH2-rLybP0QzVZz8MGKstDkOqTJ9LvSmN4z6N5fVm7kw09nYK3lVXBscQ4635iWfkqDdDwvOn-5R8__jh2_Zqdf318tN2c72ygtK8Yo2xomPQCwkWLIq2b2mjHJctRdGtUWHjHDjoitx01IrerZXsQTrBrEJ-St4ecu9i-D1jynr0yZa_mgnDnHSZsW0aRrkoUnaQ2hhSitjru-hHE_eagl6Q6p1ekOoFqQauC9JievOUP3cjumfLX4BF8O4gwDLlvceok_U4WXQ-os3aBf___Pf_2O3gJ2_N8Av3mHZhjlPhp6lOTIO-WZa67JRKDpwrxR8BsvedNw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1879662134</pqid></control><display><type>article</type><title>Activation of Cytotoxic Natural Killer Cells after Aneurysmal Subarachnoid Haemorrhage</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Spitzer, Daniel, Dr. med ; Johanna Spitzer, Nina ; Deininger, Monika ; Rainer Wirtz, Christian, Prof. Dr. med ; König, Ralph, Prof. Dr. med ; Burster, Timo, PD Dr. rer. nat ; Kapapa, Thomas, PD Dr. med</creator><creatorcontrib>Spitzer, Daniel, Dr. med ; Johanna Spitzer, Nina ; Deininger, Monika ; Rainer Wirtz, Christian, Prof. Dr. med ; König, Ralph, Prof. Dr. med ; Burster, Timo, PD Dr. rer. nat ; Kapapa, Thomas, PD Dr. med</creatorcontrib><description>Abstract Objective Cell-mediated inflammation is critical in development of cerebrovascular complications after aneurysmal subarachnoid haemorrhage (aSAH). We analysed the course for activated CD16bright CD56dim cytotoxic NKCSF cells (NK) in cerebrospinal fluid (CSF) of 15 patients. Methods Patients were classified by occurrence of cerebral vasospasm and delayed cerebral ischemia. NK were monitored by flow cytometry between day 1 and 14 after haemorrhage. Results 12 (80%) patients developed CV with a mean day of detection at 3.9±1.6. In those cell count for NK increased from 1.40±1.42 cells/μl on day 1 to a peak of 11.66±11.56 cells/μl on day 6.1±2.9 (p=0.001). An increase of mean CBFV in TCD from 71.33±12.93 cm/sec. to 166.20±20.19 cm/sec. (p<0.01) and an increase in number of vascular axes affected by CV was detected (p<0.01). In patients with grade 3 CV (n=4, 33.3%) activated NK counts were significantly higher than in non-CV patients (23.18±13.92 cells/μl versus 0.02±0.01 cells/μl; p=0.029). NK counts were significantly different between patients with grade 1 and grade 3 CV (p=0.04). Non-CV patients who demonstrated low NK counts achieved better functional outcome (GOS 4.6±0.6) at discharge than patients with CV grade 2 (GOS 3.3±0.5) and CV grade 3 (GOS 2.3±0.5) who demonstrated elevated NKC counts (CV grade 0 versus CV grade 2, p=0.048; CV grade 0 versus CV grade 3, p=0.001). Activated CD16bright CD56dim cytotoxic NKCSF cell counts revealed a mean maximum (14.15±12.21 cells/μl) when DCI occurred. Conclusion The increase of activated CD16bright CD56dim cytotoxic NK cells in CSF after aSAH suggests an increased risk of CV and DCI.</description><identifier>ISSN: 1878-8750</identifier><identifier>EISSN: 1878-8769</identifier><identifier>DOI: 10.1016/j.wneu.2017.03.026</identifier><identifier>PMID: 28323187</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; Angiography ; Cytotoxicity, Immunologic - physiology ; Delayed cerebral ischemia ; Female ; Humans ; Infarction ; Inflammation ; Killer Cells, Natural - immunology ; Killer Cells, Natural - metabolism ; Male ; Middle Aged ; Neurosurgery ; Outcome ; Prospective Studies ; Subarachnoid Hemorrhage - immunology ; Subarachnoid Hemorrhage - metabolism ; Vasospasm, Intracranial - immunology ; Vasospasm, Intracranial - metabolism</subject><ispartof>World neurosurgery, 2017-05, Vol.101, p.666-676.e1</ispartof><rights>Elsevier Inc.</rights><rights>2017 Elsevier Inc.</rights><rights>Copyright © 2017 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-26ac4b20f470c0ce49f9168d3791e4b5e8e6dd0d0b411ab1c4fd587f07d42c8e3</citedby><cites>FETCH-LOGICAL-c411t-26ac4b20f470c0ce49f9168d3791e4b5e8e6dd0d0b411ab1c4fd587f07d42c8e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1878875017303388$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28323187$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Spitzer, Daniel, Dr. med</creatorcontrib><creatorcontrib>Johanna Spitzer, Nina</creatorcontrib><creatorcontrib>Deininger, Monika</creatorcontrib><creatorcontrib>Rainer Wirtz, Christian, Prof. Dr. med</creatorcontrib><creatorcontrib>König, Ralph, Prof. Dr. med</creatorcontrib><creatorcontrib>Burster, Timo, PD Dr. rer. nat</creatorcontrib><creatorcontrib>Kapapa, Thomas, PD Dr. med</creatorcontrib><title>Activation of Cytotoxic Natural Killer Cells after Aneurysmal Subarachnoid Haemorrhage</title><title>World neurosurgery</title><addtitle>World Neurosurg</addtitle><description>Abstract Objective Cell-mediated inflammation is critical in development of cerebrovascular complications after aneurysmal subarachnoid haemorrhage (aSAH). We analysed the course for activated CD16bright CD56dim cytotoxic NKCSF cells (NK) in cerebrospinal fluid (CSF) of 15 patients. Methods Patients were classified by occurrence of cerebral vasospasm and delayed cerebral ischemia. NK were monitored by flow cytometry between day 1 and 14 after haemorrhage. Results 12 (80%) patients developed CV with a mean day of detection at 3.9±1.6. In those cell count for NK increased from 1.40±1.42 cells/μl on day 1 to a peak of 11.66±11.56 cells/μl on day 6.1±2.9 (p=0.001). An increase of mean CBFV in TCD from 71.33±12.93 cm/sec. to 166.20±20.19 cm/sec. (p<0.01) and an increase in number of vascular axes affected by CV was detected (p<0.01). In patients with grade 3 CV (n=4, 33.3%) activated NK counts were significantly higher than in non-CV patients (23.18±13.92 cells/μl versus 0.02±0.01 cells/μl; p=0.029). NK counts were significantly different between patients with grade 1 and grade 3 CV (p=0.04). Non-CV patients who demonstrated low NK counts achieved better functional outcome (GOS 4.6±0.6) at discharge than patients with CV grade 2 (GOS 3.3±0.5) and CV grade 3 (GOS 2.3±0.5) who demonstrated elevated NKC counts (CV grade 0 versus CV grade 2, p=0.048; CV grade 0 versus CV grade 3, p=0.001). Activated CD16bright CD56dim cytotoxic NKCSF cell counts revealed a mean maximum (14.15±12.21 cells/μl) when DCI occurred. Conclusion The increase of activated CD16bright CD56dim cytotoxic NK cells in CSF after aSAH suggests an increased risk of CV and DCI.</description><subject>Adult</subject><subject>Angiography</subject><subject>Cytotoxicity, Immunologic - physiology</subject><subject>Delayed cerebral ischemia</subject><subject>Female</subject><subject>Humans</subject><subject>Infarction</subject><subject>Inflammation</subject><subject>Killer Cells, Natural - immunology</subject><subject>Killer Cells, Natural - metabolism</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Neurosurgery</subject><subject>Outcome</subject><subject>Prospective Studies</subject><subject>Subarachnoid Hemorrhage - immunology</subject><subject>Subarachnoid Hemorrhage - metabolism</subject><subject>Vasospasm, Intracranial - immunology</subject><subject>Vasospasm, Intracranial - metabolism</subject><issn>1878-8750</issn><issn>1878-8769</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9v1DAQxS1ERau2X4ADypHLpuM_GzsSQlqtoEVUcChwtRx7Qr0kcbGdlv32dbSlBw744pH83hvPbwh5TaGmQJuLXf0w4VwzoLIGXgNrXpATqqRaKdm0L5_rNRyT85R2UA6nQkn-ihwzxRkvghPyY2OzvzfZh6kKfbXd55DDH2-rLybP0QzVZz8MGKstDkOqTJ9LvSmN4z6N5fVm7kw09nYK3lVXBscQ4635iWfkqDdDwvOn-5R8__jh2_Zqdf318tN2c72ygtK8Yo2xomPQCwkWLIq2b2mjHJctRdGtUWHjHDjoitx01IrerZXsQTrBrEJ-St4ecu9i-D1jynr0yZa_mgnDnHSZsW0aRrkoUnaQ2hhSitjru-hHE_eagl6Q6p1ekOoFqQauC9JievOUP3cjumfLX4BF8O4gwDLlvceok_U4WXQ-os3aBf___Pf_2O3gJ2_N8Av3mHZhjlPhp6lOTIO-WZa67JRKDpwrxR8BsvedNw</recordid><startdate>20170501</startdate><enddate>20170501</enddate><creator>Spitzer, Daniel, Dr. med</creator><creator>Johanna Spitzer, Nina</creator><creator>Deininger, Monika</creator><creator>Rainer Wirtz, Christian, Prof. Dr. med</creator><creator>König, Ralph, Prof. Dr. med</creator><creator>Burster, Timo, PD Dr. rer. nat</creator><creator>Kapapa, Thomas, PD Dr. med</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170501</creationdate><title>Activation of Cytotoxic Natural Killer Cells after Aneurysmal Subarachnoid Haemorrhage</title><author>Spitzer, Daniel, Dr. med ; Johanna Spitzer, Nina ; Deininger, Monika ; Rainer Wirtz, Christian, Prof. Dr. med ; König, Ralph, Prof. Dr. med ; Burster, Timo, PD Dr. rer. nat ; Kapapa, Thomas, PD Dr. med</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-26ac4b20f470c0ce49f9168d3791e4b5e8e6dd0d0b411ab1c4fd587f07d42c8e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Angiography</topic><topic>Cytotoxicity, Immunologic - physiology</topic><topic>Delayed cerebral ischemia</topic><topic>Female</topic><topic>Humans</topic><topic>Infarction</topic><topic>Inflammation</topic><topic>Killer Cells, Natural - immunology</topic><topic>Killer Cells, Natural - metabolism</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Neurosurgery</topic><topic>Outcome</topic><topic>Prospective Studies</topic><topic>Subarachnoid Hemorrhage - immunology</topic><topic>Subarachnoid Hemorrhage - metabolism</topic><topic>Vasospasm, Intracranial - immunology</topic><topic>Vasospasm, Intracranial - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Spitzer, Daniel, Dr. med</creatorcontrib><creatorcontrib>Johanna Spitzer, Nina</creatorcontrib><creatorcontrib>Deininger, Monika</creatorcontrib><creatorcontrib>Rainer Wirtz, Christian, Prof. Dr. med</creatorcontrib><creatorcontrib>König, Ralph, Prof. Dr. med</creatorcontrib><creatorcontrib>Burster, Timo, PD Dr. rer. nat</creatorcontrib><creatorcontrib>Kapapa, Thomas, PD Dr. med</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>World neurosurgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Spitzer, Daniel, Dr. med</au><au>Johanna Spitzer, Nina</au><au>Deininger, Monika</au><au>Rainer Wirtz, Christian, Prof. Dr. med</au><au>König, Ralph, Prof. Dr. med</au><au>Burster, Timo, PD Dr. rer. nat</au><au>Kapapa, Thomas, PD Dr. med</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activation of Cytotoxic Natural Killer Cells after Aneurysmal Subarachnoid Haemorrhage</atitle><jtitle>World neurosurgery</jtitle><addtitle>World Neurosurg</addtitle><date>2017-05-01</date><risdate>2017</risdate><volume>101</volume><spage>666</spage><epage>676.e1</epage><pages>666-676.e1</pages><issn>1878-8750</issn><eissn>1878-8769</eissn><abstract>Abstract Objective Cell-mediated inflammation is critical in development of cerebrovascular complications after aneurysmal subarachnoid haemorrhage (aSAH). We analysed the course for activated CD16bright CD56dim cytotoxic NKCSF cells (NK) in cerebrospinal fluid (CSF) of 15 patients. Methods Patients were classified by occurrence of cerebral vasospasm and delayed cerebral ischemia. NK were monitored by flow cytometry between day 1 and 14 after haemorrhage. Results 12 (80%) patients developed CV with a mean day of detection at 3.9±1.6. In those cell count for NK increased from 1.40±1.42 cells/μl on day 1 to a peak of 11.66±11.56 cells/μl on day 6.1±2.9 (p=0.001). An increase of mean CBFV in TCD from 71.33±12.93 cm/sec. to 166.20±20.19 cm/sec. (p<0.01) and an increase in number of vascular axes affected by CV was detected (p<0.01). In patients with grade 3 CV (n=4, 33.3%) activated NK counts were significantly higher than in non-CV patients (23.18±13.92 cells/μl versus 0.02±0.01 cells/μl; p=0.029). NK counts were significantly different between patients with grade 1 and grade 3 CV (p=0.04). Non-CV patients who demonstrated low NK counts achieved better functional outcome (GOS 4.6±0.6) at discharge than patients with CV grade 2 (GOS 3.3±0.5) and CV grade 3 (GOS 2.3±0.5) who demonstrated elevated NKC counts (CV grade 0 versus CV grade 2, p=0.048; CV grade 0 versus CV grade 3, p=0.001). Activated CD16bright CD56dim cytotoxic NKCSF cell counts revealed a mean maximum (14.15±12.21 cells/μl) when DCI occurred. Conclusion The increase of activated CD16bright CD56dim cytotoxic NK cells in CSF after aSAH suggests an increased risk of CV and DCI.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>28323187</pmid><doi>10.1016/j.wneu.2017.03.026</doi></addata></record> |
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| subjects | Adult Angiography Cytotoxicity, Immunologic - physiology Delayed cerebral ischemia Female Humans Infarction Inflammation Killer Cells, Natural - immunology Killer Cells, Natural - metabolism Male Middle Aged Neurosurgery Outcome Prospective Studies Subarachnoid Hemorrhage - immunology Subarachnoid Hemorrhage - metabolism Vasospasm, Intracranial - immunology Vasospasm, Intracranial - metabolism |
| title | Activation of Cytotoxic Natural Killer Cells after Aneurysmal Subarachnoid Haemorrhage |
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