Production, IMAC Purification, and Molecular Modeling of N-Carbamoyl-d-amino Acid Amidohydrolase C-Terminally Fused with a Six-His Peptide

A six‐His peptide was genetically engineered to the C‐terminus of Agrobacterium radiobacter N‐carbamoyl‐d‐amino acid amidohydrolase monomer to facilitate the protein purification with immobilized metal affinity chromatography (IMAC). The fusion enzyme, named as DCaseH, was overexpressed in Escherich...

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