Use of RNA‐seq to determine variation in canine cytochrome P450 mRNA expression between blood, liver, lung, kidney and duodenum in healthy beagles
RNA sequencing (RNA‐seq) is a powerful tool for the evaluation and quantification of transcriptomes and expression patterns in animals, tissues, or pathological conditions. The purpose of this study was to determine the physiologic expression of cytochrome P450 (CYP) mRNA transcripts in whole blood,...
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| Veröffentlicht in: | Journal of veterinary pharmacology and therapeutics 2017-12, Vol.40 (6), p.583-590 |
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| creator | Visser, M. Weber, K. Rincon, G. Merritt, D. |
| description | RNA sequencing (RNA‐seq) is a powerful tool for the evaluation and quantification of transcriptomes and expression patterns in animals, tissues, or pathological conditions. The purpose of this study was to determine the physiologic expression of cytochrome P450 (CYP) mRNA transcripts in whole blood, kidney, duodenum, liver, and lung in healthy, adult male (n = 4) and female (n = 4) beagles via RNA‐seq. mRNA expression was above background (transcripts per million) for 45 canine CYPs, with liver, duodenum, and lung expressing a high number of xenobiotic metabolizing CYPs, while prominent endogenous metabolizing CYP expression was present in blood and kidney. The relative expression pattern of CYP2A13, 2B11, 2C21, 2D15, 2E1, 3A12, and 27A1 in liver, lung, and duodenum was verified through qPCR. This is the first global profiling of physiologic CYP mRNA expression in multiple canine tissues, providing a platform for further studies characterizing canine CYPs and changes in gene expression in disease states. |
| doi_str_mv | 10.1111/jvp.12400 |
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The purpose of this study was to determine the physiologic expression of cytochrome P450 (CYP) mRNA transcripts in whole blood, kidney, duodenum, liver, and lung in healthy, adult male (n = 4) and female (n = 4) beagles via RNA‐seq. mRNA expression was above background (transcripts per million) for 45 canine CYPs, with liver, duodenum, and lung expressing a high number of xenobiotic metabolizing CYPs, while prominent endogenous metabolizing CYP expression was present in blood and kidney. The relative expression pattern of CYP2A13, 2B11, 2C21, 2D15, 2E1, 3A12, and 27A1 in liver, lung, and duodenum was verified through qPCR. 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The purpose of this study was to determine the physiologic expression of cytochrome P450 (CYP) mRNA transcripts in whole blood, kidney, duodenum, liver, and lung in healthy, adult male (n = 4) and female (n = 4) beagles via RNA‐seq. mRNA expression was above background (transcripts per million) for 45 canine CYPs, with liver, duodenum, and lung expressing a high number of xenobiotic metabolizing CYPs, while prominent endogenous metabolizing CYP expression was present in blood and kidney. The relative expression pattern of CYP2A13, 2B11, 2C21, 2D15, 2E1, 3A12, and 27A1 in liver, lung, and duodenum was verified through qPCR. This is the first global profiling of physiologic CYP mRNA expression in multiple canine tissues, providing a platform for further studies characterizing canine CYPs and changes in gene expression in disease states.</description><subject>Animals</subject><subject>Cytochrome P-450 Enzyme System - analysis</subject><subject>Cytochrome P-450 Enzyme System - blood</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>Dogs</subject><subject>Duodenum - chemistry</subject><subject>Duodenum - embryology</subject><subject>Female</subject><subject>Kidney - chemistry</subject><subject>Kidney - metabolism</subject><subject>Liver - chemistry</subject><subject>Liver - metabolism</subject><subject>Lung - chemistry</subject><subject>Lung - metabolism</subject><subject>Male</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Analysis, RNA - veterinary</subject><issn>0140-7783</issn><issn>1365-2885</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kcFO3DAQhq2qqGxpD7wA8rFIBDxxNnGOCEELQhRV0GtkO2PWNLEXO1maWx-hB56wT1IvC711Lr80-uY7zE_ILrBDSHN0v1oeQl4w9obMgJfzLBdi_pbMGBQsqyrBt8n7GO8ZY1wAvCPbueBQAS9m5Ok2IvWGfrs6_vPrd8QHOnja4oChtw7pSgYrB-sdtY5q6dY7PQ1eL4LvkV4Xc0b7dEvx5zJgjGtS4fCImLLzvj2gnV1hSDG6uwP6w7YOJypdS9vRt-jGfm1eoOyGxZRO5V2H8QPZMrKL-PEld8jt2enNyZfs8uvn85Pjy0zzGlgGUnAlGBOG57XRooJcKqlQGSMQcqZroYwwdVHWUOZSq7I0xpQG8krWqBjfIZ823mXwDyPGoelt1Nh10qEfYwOiqqGeA4OE7m9QHXyMAU2zDLaXYWqANesSmlRC81xCYvdetKPqsf1Hvn49AUcb4NF2OP3f1Fx8v94o_wLTQZM1</recordid><startdate>201712</startdate><enddate>201712</enddate><creator>Visser, M.</creator><creator>Weber, K.</creator><creator>Rincon, G.</creator><creator>Merritt, D.</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9974-2888</orcidid></search><sort><creationdate>201712</creationdate><title>Use of RNA‐seq to determine variation in canine cytochrome P450 mRNA expression between blood, liver, lung, kidney and duodenum in healthy beagles</title><author>Visser, M. ; Weber, K. ; Rincon, G. ; Merritt, D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3910-1a83b8008f329fc8712ababebff8e120c98bf8f9469162acb66fff6f127a9eb03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Cytochrome P-450 Enzyme System - analysis</topic><topic>Cytochrome P-450 Enzyme System - blood</topic><topic>Cytochrome P-450 Enzyme System - metabolism</topic><topic>Dogs</topic><topic>Duodenum - chemistry</topic><topic>Duodenum - embryology</topic><topic>Female</topic><topic>Kidney - chemistry</topic><topic>Kidney - metabolism</topic><topic>Liver - chemistry</topic><topic>Liver - metabolism</topic><topic>Lung - chemistry</topic><topic>Lung - metabolism</topic><topic>Male</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Analysis, RNA - veterinary</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Visser, M.</creatorcontrib><creatorcontrib>Weber, K.</creatorcontrib><creatorcontrib>Rincon, G.</creatorcontrib><creatorcontrib>Merritt, D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of veterinary pharmacology and therapeutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Visser, M.</au><au>Weber, K.</au><au>Rincon, G.</au><au>Merritt, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of RNA‐seq to determine variation in canine cytochrome P450 mRNA expression between blood, liver, lung, kidney and duodenum in healthy beagles</atitle><jtitle>Journal of veterinary pharmacology and therapeutics</jtitle><addtitle>J Vet Pharmacol Ther</addtitle><date>2017-12</date><risdate>2017</risdate><volume>40</volume><issue>6</issue><spage>583</spage><epage>590</epage><pages>583-590</pages><issn>0140-7783</issn><eissn>1365-2885</eissn><abstract>RNA sequencing (RNA‐seq) is a powerful tool for the evaluation and quantification of transcriptomes and expression patterns in animals, tissues, or pathological conditions. 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| issn | 0140-7783 1365-2885 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Cytochrome P-450 Enzyme System - analysis Cytochrome P-450 Enzyme System - blood Cytochrome P-450 Enzyme System - metabolism Dogs Duodenum - chemistry Duodenum - embryology Female Kidney - chemistry Kidney - metabolism Liver - chemistry Liver - metabolism Lung - chemistry Lung - metabolism Male RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Analysis, RNA - veterinary |
| title | Use of RNA‐seq to determine variation in canine cytochrome P450 mRNA expression between blood, liver, lung, kidney and duodenum in healthy beagles |
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