Stability and oligomerization of recombinant GadX, a transcriptional activator of the Escherichia coli glutamate decarboxylase system

One of the most important strategies that enteric bacteria adopt for maintaining the cytoplasmic pH neutral under acid stress involves the glutamate decarboxylase (Gad) system. The system works by the concerted action of a cytoplasmic, pyridoxal 5′-phosphate-dependent glutamate decarboxylase and a t...

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Veröffentlicht in:	Biochimica et biophysica acta 2003-04, Vol.1647 (1), p.376-380
Hauptverfasser:	Tramonti, A., De Canio, M., Bossa, F., De Biase, D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acid resistance AraC Transcription Factor - chemistry AraC/XylS-like transcriptional activator Dimerization Escherichia coli Escherichia coli Proteins - chemistry Fusion protein Glutamate decarboxylase Glutamate Decarboxylase - genetics Hydrogen-Ion Concentration Periplasmic Binding Proteins - chemistry Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification Trans-Activators - chemistry
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