Effects of supplementation of medium with different antioxidants during in vitro maturation of bovine oocytes on subsequent embryo production
Contents The aim of this study was to assess the effects of different antioxidants on the levels of reactive oxygen species (ROS) and glutathione (GSH) in oocytes during in vitro maturation (IVM), as well as on the production of embryos. Oocyte of slaughterhouse‐derived cattle ovaries were placed in...
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| Veröffentlicht in: | Reproduction in domestic animals 2017-08, Vol.52 (4), p.561-569 |
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| creator | Sovernigo, TC Adona, PR Monzani, PS Guemra, S Barros, FDA Lopes, FG Leal, CLV |
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The aim of this study was to assess the effects of different antioxidants on the levels of reactive oxygen species (ROS) and glutathione (GSH) in oocytes during in vitro maturation (IVM), as well as on the production of embryos. Oocyte of slaughterhouse‐derived cattle ovaries were placed in IVM with different antioxidants: quercetin (2 μM), cysteamine (100 μM), carnitine (0.5 mg/ml), vitamin C (50 μg/ml) or resveratrol (2 μM). Oocytes matured without any antioxidant supplementation were used as control. The oocytes were assessed for maturation rates and for ROS and GSH levels by fluorescence staining in 2′,7′‐dichlorodihydrofluorescein diacetate and Cell Tracker Blue, respectively. Embryo production was assessed in terms of cleavage, blastocysts and hatching rates and embryo cell numbers. The results expressed in arbitrary fluorescence units showed ROS reduction (p |
| doi_str_mv | 10.1111/rda.12946 |
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The aim of this study was to assess the effects of different antioxidants on the levels of reactive oxygen species (ROS) and glutathione (GSH) in oocytes during in vitro maturation (IVM), as well as on the production of embryos. Oocyte of slaughterhouse‐derived cattle ovaries were placed in IVM with different antioxidants: quercetin (2 μM), cysteamine (100 μM), carnitine (0.5 mg/ml), vitamin C (50 μg/ml) or resveratrol (2 μM). Oocytes matured without any antioxidant supplementation were used as control. The oocytes were assessed for maturation rates and for ROS and GSH levels by fluorescence staining in 2′,7′‐dichlorodihydrofluorescein diacetate and Cell Tracker Blue, respectively. Embryo production was assessed in terms of cleavage, blastocysts and hatching rates and embryo cell numbers. The results expressed in arbitrary fluorescence units showed ROS reduction (p < .05) in the groups with quercetin (27.5 ± 3.4), vitamin C (27.1 ± 3.0) or resveratrol (28.1 ± 4.7), in comparison with those with cysteamine (34.9 ± 4.5), carnitine (34.6 ± 3.8) or to the control group (36.5 ± 5.2). GSH levels increased (p < .05) in cysteamine (63.5 ± 5.5) or carnitine (60.8 ± 4.4) groups in comparison with quercetin (52.7 ± 5.1), vitamin C (53.0 ± 3.8), resveratrol (53.1 ± 4.4) or to the control (49.6 ± 4.5). Nuclear maturation cleavage and hatched blastocysts rates did not differ (p > .05) between groups. However, blastocyst rates after in vitro fertilization in quercetin (53.5 ± 3.9%), vitamin C (52.1 ± 3.1%) resveratrol (54.2 ± 4.0%), cysteamine (52.4 ± 2.7%) or carnitine (54.2 ± 3.1%) groups were higher (p < .05) than in the control (47.2 ± 2.7%). Total cell numbers in embryos from the vitamin C, resveratrol, cysteamine or carnitine groups were higher than in quercetin and control groups, which were similar to each other. The results suggest that using antioxidants during IVM may reduce oxidative stress either by decreasing ROS levels directly or by increasing GSH levels in oocytes, depending on the type of antioxidant used. Overall, oxidative stress control during IVM with the antioxidants examined here improved blastocyst development with similar efficacy.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/rda.12946</identifier><identifier>PMID: 28295710</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Animals ; Antioxidants ; Antioxidants - pharmacology ; Ascorbic acid ; Blastocyst - drug effects ; Blastocysts ; Carnitine ; Cattle ; Cysteamine ; Embryonic Development ; Embryos ; Female ; Fertilization in Vitro - veterinary ; Fluorescence ; Glutathione ; Glutathione - analysis ; Hatching ; In vitro fertilization ; In vitro methods and tests ; In Vitro Oocyte Maturation Techniques - methods ; In Vitro Oocyte Maturation Techniques - veterinary ; Male ; Maturation ; Oocytes ; Oocytes - drug effects ; Ovaries ; Oxidative stress ; Oxidative Stress - drug effects ; Quercetin ; Reactive oxygen species ; Reactive Oxygen Species - analysis ; Resveratrol ; Supplements ; Vitamin C</subject><ispartof>Reproduction in domestic animals, 2017-08, Vol.52 (4), p.561-569</ispartof><rights>2017 Blackwell Verlag GmbH</rights><rights>2017 Blackwell Verlag GmbH.</rights><rights>Copyright © 2017 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3536-19b9068f3c41a6547643627f3c5b520ae5fbddd390ed5e28ba444edd0b97a7c03</citedby><cites>FETCH-LOGICAL-c3536-19b9068f3c41a6547643627f3c5b520ae5fbddd390ed5e28ba444edd0b97a7c03</cites><orcidid>0000-0002-0936-3567</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Frda.12946$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Frda.12946$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28295710$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sovernigo, TC</creatorcontrib><creatorcontrib>Adona, PR</creatorcontrib><creatorcontrib>Monzani, PS</creatorcontrib><creatorcontrib>Guemra, S</creatorcontrib><creatorcontrib>Barros, FDA</creatorcontrib><creatorcontrib>Lopes, FG</creatorcontrib><creatorcontrib>Leal, CLV</creatorcontrib><title>Effects of supplementation of medium with different antioxidants during in vitro maturation of bovine oocytes on subsequent embryo production</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>Contents
The aim of this study was to assess the effects of different antioxidants on the levels of reactive oxygen species (ROS) and glutathione (GSH) in oocytes during in vitro maturation (IVM), as well as on the production of embryos. Oocyte of slaughterhouse‐derived cattle ovaries were placed in IVM with different antioxidants: quercetin (2 μM), cysteamine (100 μM), carnitine (0.5 mg/ml), vitamin C (50 μg/ml) or resveratrol (2 μM). Oocytes matured without any antioxidant supplementation were used as control. The oocytes were assessed for maturation rates and for ROS and GSH levels by fluorescence staining in 2′,7′‐dichlorodihydrofluorescein diacetate and Cell Tracker Blue, respectively. Embryo production was assessed in terms of cleavage, blastocysts and hatching rates and embryo cell numbers. The results expressed in arbitrary fluorescence units showed ROS reduction (p < .05) in the groups with quercetin (27.5 ± 3.4), vitamin C (27.1 ± 3.0) or resveratrol (28.1 ± 4.7), in comparison with those with cysteamine (34.9 ± 4.5), carnitine (34.6 ± 3.8) or to the control group (36.5 ± 5.2). GSH levels increased (p < .05) in cysteamine (63.5 ± 5.5) or carnitine (60.8 ± 4.4) groups in comparison with quercetin (52.7 ± 5.1), vitamin C (53.0 ± 3.8), resveratrol (53.1 ± 4.4) or to the control (49.6 ± 4.5). Nuclear maturation cleavage and hatched blastocysts rates did not differ (p > .05) between groups. However, blastocyst rates after in vitro fertilization in quercetin (53.5 ± 3.9%), vitamin C (52.1 ± 3.1%) resveratrol (54.2 ± 4.0%), cysteamine (52.4 ± 2.7%) or carnitine (54.2 ± 3.1%) groups were higher (p < .05) than in the control (47.2 ± 2.7%). Total cell numbers in embryos from the vitamin C, resveratrol, cysteamine or carnitine groups were higher than in quercetin and control groups, which were similar to each other. The results suggest that using antioxidants during IVM may reduce oxidative stress either by decreasing ROS levels directly or by increasing GSH levels in oocytes, depending on the type of antioxidant used. Overall, oxidative stress control during IVM with the antioxidants examined here improved blastocyst development with similar efficacy.</description><subject>Animals</subject><subject>Antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>Ascorbic acid</subject><subject>Blastocyst - drug effects</subject><subject>Blastocysts</subject><subject>Carnitine</subject><subject>Cattle</subject><subject>Cysteamine</subject><subject>Embryonic Development</subject><subject>Embryos</subject><subject>Female</subject><subject>Fertilization in Vitro - veterinary</subject><subject>Fluorescence</subject><subject>Glutathione</subject><subject>Glutathione - analysis</subject><subject>Hatching</subject><subject>In vitro fertilization</subject><subject>In vitro methods and tests</subject><subject>In Vitro Oocyte Maturation Techniques - methods</subject><subject>In Vitro Oocyte Maturation Techniques - veterinary</subject><subject>Male</subject><subject>Maturation</subject><subject>Oocytes</subject><subject>Oocytes - drug effects</subject><subject>Ovaries</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Quercetin</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - analysis</subject><subject>Resveratrol</subject><subject>Supplements</subject><subject>Vitamin C</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc9u1DAQxi0EotvCgRdAlrjQw7Z2EtvxseofQKqEVJWzZccTcJXYix233YfgnZllSw9I-DLyzM_fjOcj5B1nJxzPafb2hDe6ky_IinetXjPR8pdkxXQr11LJ_oAclnLHGBe9Uq_JQdM3WijOVuTX5TjCsBSaRlrqZjPBDHGxS0hxl5rBhzrTh7D8oD4gmrFKbcT6Y_AYC_U1h_idhkjvw5ITne1S87OAS_chAk1p2C6AXSJ2cQV-1p0OzC5vE93k5Ouwe_GGvBrtVODtUzwi364ub88_r6-_fvpyfna9HlqBX-LaaSb7sR06bqXolOxa2Si8CycaZkGMznvfagZeQNM723UdeM-cVlYNrD0iH_e62BpHKYuZQxlgmmyEVIvhuKZeCKE0oh_-Qe9SzRGnM1xzKaVuJEfqeE8NOZWSYTSbHGabt4Yzs_PIoEfmj0fIvn9SrA73-0z-NQWB0z3wECbY_l_J3Fyc7SV_AwGunmk</recordid><startdate>201708</startdate><enddate>201708</enddate><creator>Sovernigo, TC</creator><creator>Adona, PR</creator><creator>Monzani, PS</creator><creator>Guemra, S</creator><creator>Barros, FDA</creator><creator>Lopes, FG</creator><creator>Leal, CLV</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0936-3567</orcidid></search><sort><creationdate>201708</creationdate><title>Effects of supplementation of medium with different antioxidants during in vitro maturation of bovine oocytes on subsequent embryo production</title><author>Sovernigo, TC ; Adona, PR ; Monzani, PS ; Guemra, S ; Barros, FDA ; Lopes, FG ; Leal, CLV</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3536-19b9068f3c41a6547643627f3c5b520ae5fbddd390ed5e28ba444edd0b97a7c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Antioxidants</topic><topic>Antioxidants - pharmacology</topic><topic>Ascorbic acid</topic><topic>Blastocyst - drug effects</topic><topic>Blastocysts</topic><topic>Carnitine</topic><topic>Cattle</topic><topic>Cysteamine</topic><topic>Embryonic Development</topic><topic>Embryos</topic><topic>Female</topic><topic>Fertilization in Vitro - veterinary</topic><topic>Fluorescence</topic><topic>Glutathione</topic><topic>Glutathione - analysis</topic><topic>Hatching</topic><topic>In vitro fertilization</topic><topic>In vitro methods and tests</topic><topic>In Vitro Oocyte Maturation Techniques - methods</topic><topic>In Vitro Oocyte Maturation Techniques - veterinary</topic><topic>Male</topic><topic>Maturation</topic><topic>Oocytes</topic><topic>Oocytes - drug effects</topic><topic>Ovaries</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Quercetin</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - analysis</topic><topic>Resveratrol</topic><topic>Supplements</topic><topic>Vitamin C</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sovernigo, TC</creatorcontrib><creatorcontrib>Adona, PR</creatorcontrib><creatorcontrib>Monzani, PS</creatorcontrib><creatorcontrib>Guemra, S</creatorcontrib><creatorcontrib>Barros, FDA</creatorcontrib><creatorcontrib>Lopes, FG</creatorcontrib><creatorcontrib>Leal, CLV</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sovernigo, TC</au><au>Adona, PR</au><au>Monzani, PS</au><au>Guemra, S</au><au>Barros, FDA</au><au>Lopes, FG</au><au>Leal, CLV</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of supplementation of medium with different antioxidants during in vitro maturation of bovine oocytes on subsequent embryo production</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2017-08</date><risdate>2017</risdate><volume>52</volume><issue>4</issue><spage>561</spage><epage>569</epage><pages>561-569</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>Contents
The aim of this study was to assess the effects of different antioxidants on the levels of reactive oxygen species (ROS) and glutathione (GSH) in oocytes during in vitro maturation (IVM), as well as on the production of embryos. Oocyte of slaughterhouse‐derived cattle ovaries were placed in IVM with different antioxidants: quercetin (2 μM), cysteamine (100 μM), carnitine (0.5 mg/ml), vitamin C (50 μg/ml) or resveratrol (2 μM). Oocytes matured without any antioxidant supplementation were used as control. The oocytes were assessed for maturation rates and for ROS and GSH levels by fluorescence staining in 2′,7′‐dichlorodihydrofluorescein diacetate and Cell Tracker Blue, respectively. Embryo production was assessed in terms of cleavage, blastocysts and hatching rates and embryo cell numbers. The results expressed in arbitrary fluorescence units showed ROS reduction (p < .05) in the groups with quercetin (27.5 ± 3.4), vitamin C (27.1 ± 3.0) or resveratrol (28.1 ± 4.7), in comparison with those with cysteamine (34.9 ± 4.5), carnitine (34.6 ± 3.8) or to the control group (36.5 ± 5.2). GSH levels increased (p < .05) in cysteamine (63.5 ± 5.5) or carnitine (60.8 ± 4.4) groups in comparison with quercetin (52.7 ± 5.1), vitamin C (53.0 ± 3.8), resveratrol (53.1 ± 4.4) or to the control (49.6 ± 4.5). Nuclear maturation cleavage and hatched blastocysts rates did not differ (p > .05) between groups. However, blastocyst rates after in vitro fertilization in quercetin (53.5 ± 3.9%), vitamin C (52.1 ± 3.1%) resveratrol (54.2 ± 4.0%), cysteamine (52.4 ± 2.7%) or carnitine (54.2 ± 3.1%) groups were higher (p < .05) than in the control (47.2 ± 2.7%). Total cell numbers in embryos from the vitamin C, resveratrol, cysteamine or carnitine groups were higher than in quercetin and control groups, which were similar to each other. The results suggest that using antioxidants during IVM may reduce oxidative stress either by decreasing ROS levels directly or by increasing GSH levels in oocytes, depending on the type of antioxidant used. Overall, oxidative stress control during IVM with the antioxidants examined here improved blastocyst development with similar efficacy.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>28295710</pmid><doi>10.1111/rda.12946</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-0936-3567</orcidid></addata></record> |
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| subjects | Animals Antioxidants Antioxidants - pharmacology Ascorbic acid Blastocyst - drug effects Blastocysts Carnitine Cattle Cysteamine Embryonic Development Embryos Female Fertilization in Vitro - veterinary Fluorescence Glutathione Glutathione - analysis Hatching In vitro fertilization In vitro methods and tests In Vitro Oocyte Maturation Techniques - methods In Vitro Oocyte Maturation Techniques - veterinary Male Maturation Oocytes Oocytes - drug effects Ovaries Oxidative stress Oxidative Stress - drug effects Quercetin Reactive oxygen species Reactive Oxygen Species - analysis Resveratrol Supplements Vitamin C |
| title | Effects of supplementation of medium with different antioxidants during in vitro maturation of bovine oocytes on subsequent embryo production |
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