Confirmation of Peronospora agrimoniae as a distinct species

Leaves with typical symptoms of downy mildew were found on common agrimony in the Czech Republic in 2014 and 2015 and at several locations in Germany from 2010 to 2014. The causal agent of downy mildew of agrimony was often reported as Peronospora agrimoniae , but sometimes also as P. sparsa . Morphological characteristics of the pathogens found in both countries are in the range of previous works for P. agrimoniae , but also other downy mildews parasitic on Rosaceae, rendering their discrimination based on published observations difficult. For molecular identification sequencing of several loci (nrITS rDNA, cox 1 and cox 2) was performed. Phylogenetic analyses based on nrITS rDNA clearly separated P. agrimoniae from other Peronospora species infecting Rosaceae. Thus, considering P. agrimoniae as separate species seems justified. Two German specimens were identical to two Czech samples in both nrITS rDNA and cox 1 mtDNA sequences, but differed in a single nucleotide substitution in cox 2 region. To our knowledge, this is the first verified record of P. agrimoniae on common agrimony in the Czech Republic.