In vitro and in vivo evaluation of the probiotic attributes of Lactobacillus kefiranofaciens XL10 isolated from Tibetan kefir grain

Lactobacillus kefiranofaciens XL10, with a high yield of extracellular polysaccharide (EPS), is isolated from Tibetan kefir grain and benefits the health of human beings and has been considered to exhibit probiotic potential in vitro and in vivo. The probiotic function of the strain was studied exte...

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Veröffentlicht in:Applied microbiology and biotechnology 2017-03, Vol.101 (6), p.2467-2477
Hauptverfasser: Xing, Zhuqing, Tang, Wei, Geng, Weitao, Zheng, Yongna, Wang, Yanping
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Tang, Wei
Geng, Weitao
Zheng, Yongna
Wang, Yanping
description Lactobacillus kefiranofaciens XL10, with a high yield of extracellular polysaccharide (EPS), is isolated from Tibetan kefir grain and benefits the health of human beings and has been considered to exhibit probiotic potential in vitro and in vivo. The probiotic function of the strain was studied extensively, viz., acid and bile salt tolerances, cell surface hydrophobicity and autoaggregation, the modulation of gut microbiota, and the distribution and colonization of XL10 in the mouse intestinal tract after oral administration. XL10 could survive 3-h incubation at pH 3.5 and exhibited cell surface hydrophobicity of ∼79.9% and autoaggregation of ∼27.8%. After continuous oral administration of XL10 for 2 weeks, the Bifidobacteriaceae family increased, accompanied by an observable decline in Proteobacteria phyla in the tested mice. Butyrivibrio and Pseudobutyrivibrio , recognized as butyric acid-producing bacteria, could also be detected at day 7 and day 14, respectively. The most abundant community in the mouse gut had formed by day 14. Additionally, we found that XL10 successfully adhered to the mucous tissue and colonized the ileum of the mice based on fluorescence imaging, flow cytometry, and qPCR. Our results suggested that XL10 has excellent probiotic properties and represents an alternative for exploitation in the development of novel functional foods.
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The probiotic function of the strain was studied extensively, viz., acid and bile salt tolerances, cell surface hydrophobicity and autoaggregation, the modulation of gut microbiota, and the distribution and colonization of XL10 in the mouse intestinal tract after oral administration. XL10 could survive 3-h incubation at pH 3.5 and exhibited cell surface hydrophobicity of ∼79.9% and autoaggregation of ∼27.8%. After continuous oral administration of XL10 for 2 weeks, the Bifidobacteriaceae family increased, accompanied by an observable decline in Proteobacteria phyla in the tested mice. Butyrivibrio and Pseudobutyrivibrio , recognized as butyric acid-producing bacteria, could also be detected at day 7 and day 14, respectively. The most abundant community in the mouse gut had formed by day 14. Additionally, we found that XL10 successfully adhered to the mucous tissue and colonized the ileum of the mice based on fluorescence imaging, flow cytometry, and qPCR. Our results suggested that XL10 has excellent probiotic properties and represents an alternative for exploitation in the development of novel functional foods.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>27909744</pmid><doi>10.1007/s00253-016-7956-z</doi><tpages>11</tpages></addata></record>
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subjects Animals
Applied Microbial and Cell Physiology
Bacteria
Bacteria - classification
Bacteria - drug effects
Bacteria - genetics
Bacteria - growth & development
Bacterial Adhesion
Bile
Bile Acids and Salts - pharmacology
Biomedical and Life Sciences
Biotechnology
Deoxyribonucleic acid
Dilution
DNA
Edible Grain - microbiology
Flow cytometry
Functional foods
Functional foods & nutraceuticals
Gastrointestinal Microbiome - drug effects
Gastrointestinal Microbiome - genetics
Humans
Hydrogen-Ion Concentration
Intestines - drug effects
Intestines - microbiology
Intestines - physiology
Kefir - microbiology
Lactobacillus
Lactobacillus - physiology
Life Sciences
Male
Mice
Mice, Inbred BALB C
Microbial Genetics and Genomics
Microbiology
Microbiota
Microbiota (Symbiotic organisms)
Morphology
Novel foods
Oral administration
Phylogeny
Polysaccharides
Probiotics
Probiotics - pharmacology
Proteobacteria
RNA, Ribosomal, 16S - genetics
Salt
Surface Properties
title In vitro and in vivo evaluation of the probiotic attributes of Lactobacillus kefiranofaciens XL10 isolated from Tibetan kefir grain
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