Analysis of 12 X-STR loci in the population of south Croatia
The aim of the study was to assess forensic pertinence of 12 short tandem repeats (STRs) on X-chromosome in south Croatia population. Investigator ® Argus X-12 kit was used to co-amplify 12 STR loci belonging to four linkage groups (LGs) on X-chromosome in 99 male and 98 female DNA samples of unrela...
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| Veröffentlicht in: | Molecular biology reports 2017-02, Vol.44 (1), p.183-189 |
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| creator | Mršić, Gordan Ozretić, Petar Crnjac, Josip Merkaš, Siniša Račić, Ivana Rožić, Sara Sukser, Viktorija Popović, Maja Korolija, Marina |
| description | The aim of the study was to assess forensic pertinence of 12 short tandem repeats (STRs) on X-chromosome in south Croatia population. Investigator
®
Argus X-12 kit was used to co-amplify 12 STR loci belonging to four linkage groups (LGs) on X-chromosome in 99 male and 98 female DNA samples of unrelated donors. PCR products were analyzed by capillary electrophoresis. Population genetic and forensic parameters were calculated by the Arlequin and POPTREE2 software, and an on-line tool available at ChrX-STR.org. Hardy–Weinberg equilibrium was confirmed for all X-STR markers in female samples. Biallelic patterns at DXS10079 locus were detected in four male samples. Polymorphism information content for the most (DXS10135) and the least (DXS8378) informative markers was 0.9212 and 0.6347, respectively. In both male and female samples, combined power of discrimination exceeded 0.999999999. As confirmed by linkage disequilibrium test, significant association of marker pair DXS10074-DXS10079 (P = 0.0004) within LG2 and marker pair DXS10101-DXS10103 (P = 0.0003) within LG3 was found only in male samples. Number of observed haplotypes in our sample pool amounted 3.01, 7.53, 5 and 3.25% of the number of possible haplotypes for LG1, LG2, LG3 and LG4, respectively. According to haplotype diversity value of 0.9981, LG1 was the most informative. In comparison of south Croatia with 26 world populations, pair-wise
F
ST
*
values increase in parallel with geographical distance. Overall statistical assessment confirmed suitability of Investigator
®
Argus X-12 kit for forensic casework in both identification and familial testing in the population of south Croatia. |
| doi_str_mv | 10.1007/s11033-017-4096-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1877836532</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>4314268101</sourcerecordid><originalsourceid>FETCH-LOGICAL-c405t-d45912a6452431f315a6e4f093489d7196b06e21c84e707b9fafa9874105e213</originalsourceid><addsrcrecordid>eNqNkctKAzEUhoMoWi8P4EYG3LiJnjO5g5tSvEFB0C7chXSasSPTSZ3MLHx7M7SKCIJkETj58iX8PyGnCJcIoK4iIjBGARXlYCTFHTJCoRjlRuldMgIGSLkWeEAOY3wDAI5K7JODXINKS47I9bhx9UesYhbKDPPshT7PnrI6FFVWNVm39Nk6rPvadVVoBiSGvltmkzakiTsme6Wroz_Z7kdkdnszm9zT6ePdw2Q8pQUH0dEFFwZzJ7nIOcOSoXDS8xIM49osFBo5B-lzLDT36VtzU7rSGa04gkhjdkQuNtp1G957Hzu7qmLh69o1PvTRolZKMylY_g80xaMA5GA9_4W-hb5NaQyU1IIzhiZRuKGKNsTY-tKu22rl2g-LYIcS7KYEm0qwQwl2MJ9tzf185RffN75ST0C-AWI6al59--PpP62fOGuMuw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1868543319</pqid></control><display><type>article</type><title>Analysis of 12 X-STR loci in the population of south Croatia</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Mršić, Gordan ; Ozretić, Petar ; Crnjac, Josip ; Merkaš, Siniša ; Račić, Ivana ; Rožić, Sara ; Sukser, Viktorija ; Popović, Maja ; Korolija, Marina</creator><creatorcontrib>Mršić, Gordan ; Ozretić, Petar ; Crnjac, Josip ; Merkaš, Siniša ; Račić, Ivana ; Rožić, Sara ; Sukser, Viktorija ; Popović, Maja ; Korolija, Marina</creatorcontrib><description>The aim of the study was to assess forensic pertinence of 12 short tandem repeats (STRs) on X-chromosome in south Croatia population. Investigator
®
Argus X-12 kit was used to co-amplify 12 STR loci belonging to four linkage groups (LGs) on X-chromosome in 99 male and 98 female DNA samples of unrelated donors. PCR products were analyzed by capillary electrophoresis. Population genetic and forensic parameters were calculated by the Arlequin and POPTREE2 software, and an on-line tool available at ChrX-STR.org. Hardy–Weinberg equilibrium was confirmed for all X-STR markers in female samples. Biallelic patterns at DXS10079 locus were detected in four male samples. Polymorphism information content for the most (DXS10135) and the least (DXS8378) informative markers was 0.9212 and 0.6347, respectively. In both male and female samples, combined power of discrimination exceeded 0.999999999. As confirmed by linkage disequilibrium test, significant association of marker pair DXS10074-DXS10079 (P = 0.0004) within LG2 and marker pair DXS10101-DXS10103 (P = 0.0003) within LG3 was found only in male samples. Number of observed haplotypes in our sample pool amounted 3.01, 7.53, 5 and 3.25% of the number of possible haplotypes for LG1, LG2, LG3 and LG4, respectively. According to haplotype diversity value of 0.9981, LG1 was the most informative. In comparison of south Croatia with 26 world populations, pair-wise
F
ST
*
values increase in parallel with geographical distance. Overall statistical assessment confirmed suitability of Investigator
®
Argus X-12 kit for forensic casework in both identification and familial testing in the population of south Croatia.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-017-4096-1</identifier><identifier>PMID: 28070706</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Biomedical and Life Sciences ; Chromosomes ; Chromosomes, Human, X - genetics ; Croatia ; European Continental Ancestry Group - genetics ; Female ; Forensic sciences ; Gene Frequency ; Genetics, Population - methods ; Haplotypes ; Histology ; Humans ; Life Sciences ; Linkage Disequilibrium ; Male ; Microsatellite Repeats ; Molecular biology ; Morphology ; Original Article</subject><ispartof>Molecular biology reports, 2017-02, Vol.44 (1), p.183-189</ispartof><rights>Springer Science+Business Media Dordrecht 2017</rights><rights>Molecular Biology Reports is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-d45912a6452431f315a6e4f093489d7196b06e21c84e707b9fafa9874105e213</citedby><cites>FETCH-LOGICAL-c405t-d45912a6452431f315a6e4f093489d7196b06e21c84e707b9fafa9874105e213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-017-4096-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-017-4096-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28070706$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mršić, Gordan</creatorcontrib><creatorcontrib>Ozretić, Petar</creatorcontrib><creatorcontrib>Crnjac, Josip</creatorcontrib><creatorcontrib>Merkaš, Siniša</creatorcontrib><creatorcontrib>Račić, Ivana</creatorcontrib><creatorcontrib>Rožić, Sara</creatorcontrib><creatorcontrib>Sukser, Viktorija</creatorcontrib><creatorcontrib>Popović, Maja</creatorcontrib><creatorcontrib>Korolija, Marina</creatorcontrib><title>Analysis of 12 X-STR loci in the population of south Croatia</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>The aim of the study was to assess forensic pertinence of 12 short tandem repeats (STRs) on X-chromosome in south Croatia population. Investigator
®
Argus X-12 kit was used to co-amplify 12 STR loci belonging to four linkage groups (LGs) on X-chromosome in 99 male and 98 female DNA samples of unrelated donors. PCR products were analyzed by capillary electrophoresis. Population genetic and forensic parameters were calculated by the Arlequin and POPTREE2 software, and an on-line tool available at ChrX-STR.org. Hardy–Weinberg equilibrium was confirmed for all X-STR markers in female samples. Biallelic patterns at DXS10079 locus were detected in four male samples. Polymorphism information content for the most (DXS10135) and the least (DXS8378) informative markers was 0.9212 and 0.6347, respectively. In both male and female samples, combined power of discrimination exceeded 0.999999999. As confirmed by linkage disequilibrium test, significant association of marker pair DXS10074-DXS10079 (P = 0.0004) within LG2 and marker pair DXS10101-DXS10103 (P = 0.0003) within LG3 was found only in male samples. Number of observed haplotypes in our sample pool amounted 3.01, 7.53, 5 and 3.25% of the number of possible haplotypes for LG1, LG2, LG3 and LG4, respectively. According to haplotype diversity value of 0.9981, LG1 was the most informative. In comparison of south Croatia with 26 world populations, pair-wise
F
ST
*
values increase in parallel with geographical distance. Overall statistical assessment confirmed suitability of Investigator
®
Argus X-12 kit for forensic casework in both identification and familial testing in the population of south Croatia.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Chromosomes</subject><subject>Chromosomes, Human, X - genetics</subject><subject>Croatia</subject><subject>European Continental Ancestry Group - genetics</subject><subject>Female</subject><subject>Forensic sciences</subject><subject>Gene Frequency</subject><subject>Genetics, Population - methods</subject><subject>Haplotypes</subject><subject>Histology</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Linkage Disequilibrium</subject><subject>Male</subject><subject>Microsatellite Repeats</subject><subject>Molecular biology</subject><subject>Morphology</subject><subject>Original Article</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkctKAzEUhoMoWi8P4EYG3LiJnjO5g5tSvEFB0C7chXSasSPTSZ3MLHx7M7SKCIJkETj58iX8PyGnCJcIoK4iIjBGARXlYCTFHTJCoRjlRuldMgIGSLkWeEAOY3wDAI5K7JODXINKS47I9bhx9UesYhbKDPPshT7PnrI6FFVWNVm39Nk6rPvadVVoBiSGvltmkzakiTsme6Wroz_Z7kdkdnszm9zT6ePdw2Q8pQUH0dEFFwZzJ7nIOcOSoXDS8xIM49osFBo5B-lzLDT36VtzU7rSGa04gkhjdkQuNtp1G957Hzu7qmLh69o1PvTRolZKMylY_g80xaMA5GA9_4W-hb5NaQyU1IIzhiZRuKGKNsTY-tKu22rl2g-LYIcS7KYEm0qwQwl2MJ9tzf185RffN75ST0C-AWI6al59--PpP62fOGuMuw</recordid><startdate>20170201</startdate><enddate>20170201</enddate><creator>Mršić, Gordan</creator><creator>Ozretić, Petar</creator><creator>Crnjac, Josip</creator><creator>Merkaš, Siniša</creator><creator>Račić, Ivana</creator><creator>Rožić, Sara</creator><creator>Sukser, Viktorija</creator><creator>Popović, Maja</creator><creator>Korolija, Marina</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20170201</creationdate><title>Analysis of 12 X-STR loci in the population of south Croatia</title><author>Mršić, Gordan ; Ozretić, Petar ; Crnjac, Josip ; Merkaš, Siniša ; Račić, Ivana ; Rožić, Sara ; Sukser, Viktorija ; Popović, Maja ; Korolija, Marina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-d45912a6452431f315a6e4f093489d7196b06e21c84e707b9fafa9874105e213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Chromosomes</topic><topic>Chromosomes, Human, X - genetics</topic><topic>Croatia</topic><topic>European Continental Ancestry Group - genetics</topic><topic>Female</topic><topic>Forensic sciences</topic><topic>Gene Frequency</topic><topic>Genetics, Population - methods</topic><topic>Haplotypes</topic><topic>Histology</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Linkage Disequilibrium</topic><topic>Male</topic><topic>Microsatellite Repeats</topic><topic>Molecular biology</topic><topic>Morphology</topic><topic>Original Article</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mršić, Gordan</creatorcontrib><creatorcontrib>Ozretić, Petar</creatorcontrib><creatorcontrib>Crnjac, Josip</creatorcontrib><creatorcontrib>Merkaš, Siniša</creatorcontrib><creatorcontrib>Račić, Ivana</creatorcontrib><creatorcontrib>Rožić, Sara</creatorcontrib><creatorcontrib>Sukser, Viktorija</creatorcontrib><creatorcontrib>Popović, Maja</creatorcontrib><creatorcontrib>Korolija, Marina</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mršić, Gordan</au><au>Ozretić, Petar</au><au>Crnjac, Josip</au><au>Merkaš, Siniša</au><au>Račić, Ivana</au><au>Rožić, Sara</au><au>Sukser, Viktorija</au><au>Popović, Maja</au><au>Korolija, Marina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of 12 X-STR loci in the population of south Croatia</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2017-02-01</date><risdate>2017</risdate><volume>44</volume><issue>1</issue><spage>183</spage><epage>189</epage><pages>183-189</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>The aim of the study was to assess forensic pertinence of 12 short tandem repeats (STRs) on X-chromosome in south Croatia population. Investigator
®
Argus X-12 kit was used to co-amplify 12 STR loci belonging to four linkage groups (LGs) on X-chromosome in 99 male and 98 female DNA samples of unrelated donors. PCR products were analyzed by capillary electrophoresis. Population genetic and forensic parameters were calculated by the Arlequin and POPTREE2 software, and an on-line tool available at ChrX-STR.org. Hardy–Weinberg equilibrium was confirmed for all X-STR markers in female samples. Biallelic patterns at DXS10079 locus were detected in four male samples. Polymorphism information content for the most (DXS10135) and the least (DXS8378) informative markers was 0.9212 and 0.6347, respectively. In both male and female samples, combined power of discrimination exceeded 0.999999999. As confirmed by linkage disequilibrium test, significant association of marker pair DXS10074-DXS10079 (P = 0.0004) within LG2 and marker pair DXS10101-DXS10103 (P = 0.0003) within LG3 was found only in male samples. Number of observed haplotypes in our sample pool amounted 3.01, 7.53, 5 and 3.25% of the number of possible haplotypes for LG1, LG2, LG3 and LG4, respectively. According to haplotype diversity value of 0.9981, LG1 was the most informative. In comparison of south Croatia with 26 world populations, pair-wise
F
ST
*
values increase in parallel with geographical distance. Overall statistical assessment confirmed suitability of Investigator
®
Argus X-12 kit for forensic casework in both identification and familial testing in the population of south Croatia.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>28070706</pmid><doi>10.1007/s11033-017-4096-1</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Molecular biology reports, 2017-02, Vol.44 (1), p.183-189 |
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| subjects | Animal Anatomy Animal Biochemistry Biomedical and Life Sciences Chromosomes Chromosomes, Human, X - genetics Croatia European Continental Ancestry Group - genetics Female Forensic sciences Gene Frequency Genetics, Population - methods Haplotypes Histology Humans Life Sciences Linkage Disequilibrium Male Microsatellite Repeats Molecular biology Morphology Original Article |
| title | Analysis of 12 X-STR loci in the population of south Croatia |
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