A simple method for developing an infectious cDNA clone of Japanese encephalitis virus

Flavivirus cDNA clones frequently demonstrate genetic instability in transformed bacteria, which hampers the construction and manipulation of cDNAs for infectious flaviviruses. In this study, we developed a stable, full-length cDNA clone, pJEHEN, of a GI JEV strain HEN0701 using a medium-copy-number...

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Veröffentlicht in:	Virus genes 2017-02, Vol.53 (1), p.4-14
Hauptverfasser:	Zheng, Hao, Zheng, Xuchen, Tong, Wu, Liu, Fei, Liang, Chao, Wang, Tao, Gao, Fei, Li, Liwei, Shan, Tongling, Li, Guoxin, Tong, Guangzhi
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biomedical and Life Sciences Biomedicine Cell Line Disease Models, Animal DNA, Complementary Encephalitis Virus, Japanese - genetics Encephalitis, Japanese - mortality Encephalitis, Japanese - virology Flaviviridae Flavivirus Gene Order Genome, Viral Medical Microbiology Mice Mutation Plant Sciences RNA, Viral Transfection Virology Virulence Virus Replication
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