Neurotoxicological characterization of fractionated polypeptide from the Sea Anemone Gyrostoma helianthus on male albino rat

Neurotoxicological characterization of fractionated polypeptide from the Sea Anemone Gyrostoma helianthus on male albino rat

Marine organisms represent a valuable source of new compounds. The present study focused on the neurotoxicity of ethanolic crude extract and partially purified protein of Anemone species collected from the Red Sea shore of Jeddah, Saudia Arabia. The ethanolic extracts were found toxic to rats and th...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of environmental biology 2016-11, Vol.37 (6), p.1385-1391
Hauptverfasser: Al-Hazimi, Mansour A, Somma, M N, Waggas, A S, Rawi, Sayed M
Format: Artikel
Sprache:eng
Schlagworte:
Edema
Environmental science
Gyrostoma
Helianthus
Light microscopy
Marine organisms
Muscles
Neurotoxicity
Toxins
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 1391
container_issue 6
container_start_page 1385
container_title Journal of environmental biology
container_volume 37
creator Al-Hazimi, Mansour A
Somma, M N
Waggas, A S
Rawi, Sayed M
description Marine organisms represent a valuable source of new compounds. The present study focused on the neurotoxicity of ethanolic crude extract and partially purified protein of Anemone species collected from the Red Sea shore of Jeddah, Saudia Arabia. The ethanolic extracts were found toxic to rats and the median lethal dose (LD50) was 20.3 mg kg b. wt. after IP of the crude extract. The rats showed behavioral changes suchas tonic convulsions, paralysis and flexing of muscles. A single dose equal to ¼ LD50 administered IP, the crude extract induced differential and time-dependentdopamine (DA), norepinephrine (NE), and serotonin (5-HT)neuronal neurotransmitter changes; reduced glutathione level(P< 0.01)and highly affected cerebral histopathological disturbances. The crude extract and peptide fraction of 1KDa showed highest norepinephrine (P
doi_str_mv 10.22438/jeb/37/6/mrn-280
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1877817847</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>4313935601</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2160-4b1e94cffa91658bbf37ee49bceca83812e4585e1287431d03c03f40dd0f11f53</originalsourceid><addsrcrecordid>eNpdkU1r3DAQhkVpoEu6PyA3QS65uKsvW_IxhCYpLO2hzVnI8iirRbZcSYZsyY-Ptumpc5lBeniZ4UHoipIvjAmudkcYdlzuut2U5oYp8gFtGO9FQ7jsP6INYa1olCTiE9rmfCS1eM9k22_Q63dYUyzxxdsY4rO3JmB7MMnYAsn_McXHGUeH3fmlzqbAiJcYTgssxY9QP-KEywHwTzD4doYpzoAfTinmEieDDxC8mcthzbgGTSYANmHwc8TJlM_owpmQYfuvX6Kn-6-_7h6b_Y-Hb3e3-8Yy2pFGDBR6YZ0zPe1aNQyOSwDRDxasUVxRBqJVLVCmpOB0JNwS7gQZR-IodS2_RDfvuUuKv1fIRU8-WwjBzBDXrKmSUlGphKzo9X_oMa5prttVqlNMdVKcA-k7ZeudOYHTS_KTSSdNif6rRFclmkvd6apEVyX8DTh-gvM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1868286745</pqid></control><display><type>article</type><title>Neurotoxicological characterization of fractionated polypeptide from the Sea Anemone Gyrostoma helianthus on male albino rat</title><source>EZB Electronic Journals Library</source><creator>Al-Hazimi, Mansour A ; Somma, M N ; Waggas, A S ; Rawi, Sayed M</creator><creatorcontrib>Al-Hazimi, Mansour A ; Somma, M N ; Waggas, A S ; Rawi, Sayed M ; King Abdulaziz University</creatorcontrib><description>Marine organisms represent a valuable source of new compounds. The present study focused on the neurotoxicity of ethanolic crude extract and partially purified protein of Anemone species collected from the Red Sea shore of Jeddah, Saudia Arabia. The ethanolic extracts were found toxic to rats and the median lethal dose (LD50) was 20.3 mg kg b. wt. after IP of the crude extract. The rats showed behavioral changes suchas tonic convulsions, paralysis and flexing of muscles. A single dose equal to ¼ LD50 administered IP, the crude extract induced differential and time-dependentdopamine (DA), norepinephrine (NE), and serotonin (5-HT)neuronal neurotransmitter changes; reduced glutathione level(P&lt; 0.01)and highly affected cerebral histopathological disturbances. The crude extract and peptide fraction of 1KDa showed highest norepinephrine (P &lt;0.01) and dopamine (P&lt;0.01) stimulatory activities. Whereas protein fraction of 500Da resulted in maximal increase (P &lt;0.01) in serotonin post 30 and 60 min. In addition, the studied toxins vigorously affected rat brain cerebral cortex histological structure, the crude extract as well as the protein fraction of 1KDa disclosed the most histopathological effects. Light microscopy revealed neural hemorrhage, formation of pycnotic nuclei, marked edema due to enlargement of Virchow, congestion of blood vessels and focal hemorrhage.</description><identifier>ISSN: 0254-8704</identifier><identifier>EISSN: 2394-0379</identifier><identifier>DOI: 10.22438/jeb/37/6/mrn-280</identifier><language>eng</language><publisher>Lucknow: Triveni Enterprises</publisher><subject>Edema ; Environmental science ; Gyrostoma ; Helianthus ; Light microscopy ; Marine organisms ; Muscles ; Neurotoxicity ; Toxins</subject><ispartof>Journal of environmental biology, 2016-11, Vol.37 (6), p.1385-1391</ispartof><rights>Copyright Triveni Enterprises Nov 2016</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Al-Hazimi, Mansour A</creatorcontrib><creatorcontrib>Somma, M N</creatorcontrib><creatorcontrib>Waggas, A S</creatorcontrib><creatorcontrib>Rawi, Sayed M</creatorcontrib><creatorcontrib>King Abdulaziz University</creatorcontrib><title>Neurotoxicological characterization of fractionated polypeptide from the Sea Anemone Gyrostoma helianthus on male albino rat</title><title>Journal of environmental biology</title><description>Marine organisms represent a valuable source of new compounds. The present study focused on the neurotoxicity of ethanolic crude extract and partially purified protein of Anemone species collected from the Red Sea shore of Jeddah, Saudia Arabia. The ethanolic extracts were found toxic to rats and the median lethal dose (LD50) was 20.3 mg kg b. wt. after IP of the crude extract. The rats showed behavioral changes suchas tonic convulsions, paralysis and flexing of muscles. A single dose equal to ¼ LD50 administered IP, the crude extract induced differential and time-dependentdopamine (DA), norepinephrine (NE), and serotonin (5-HT)neuronal neurotransmitter changes; reduced glutathione level(P&lt; 0.01)and highly affected cerebral histopathological disturbances. The crude extract and peptide fraction of 1KDa showed highest norepinephrine (P &lt;0.01) and dopamine (P&lt;0.01) stimulatory activities. Whereas protein fraction of 500Da resulted in maximal increase (P &lt;0.01) in serotonin post 30 and 60 min. In addition, the studied toxins vigorously affected rat brain cerebral cortex histological structure, the crude extract as well as the protein fraction of 1KDa disclosed the most histopathological effects. Light microscopy revealed neural hemorrhage, formation of pycnotic nuclei, marked edema due to enlargement of Virchow, congestion of blood vessels and focal hemorrhage.</description><subject>Edema</subject><subject>Environmental science</subject><subject>Gyrostoma</subject><subject>Helianthus</subject><subject>Light microscopy</subject><subject>Marine organisms</subject><subject>Muscles</subject><subject>Neurotoxicity</subject><subject>Toxins</subject><issn>0254-8704</issn><issn>2394-0379</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkU1r3DAQhkVpoEu6PyA3QS65uKsvW_IxhCYpLO2hzVnI8iirRbZcSYZsyY-Ptumpc5lBeniZ4UHoipIvjAmudkcYdlzuut2U5oYp8gFtGO9FQ7jsP6INYa1olCTiE9rmfCS1eM9k22_Q63dYUyzxxdsY4rO3JmB7MMnYAsn_McXHGUeH3fmlzqbAiJcYTgssxY9QP-KEywHwTzD4doYpzoAfTinmEieDDxC8mcthzbgGTSYANmHwc8TJlM_owpmQYfuvX6Kn-6-_7h6b_Y-Hb3e3-8Yy2pFGDBR6YZ0zPe1aNQyOSwDRDxasUVxRBqJVLVCmpOB0JNwS7gQZR-IodS2_RDfvuUuKv1fIRU8-WwjBzBDXrKmSUlGphKzo9X_oMa5prttVqlNMdVKcA-k7ZeudOYHTS_KTSSdNif6rRFclmkvd6apEVyX8DTh-gvM</recordid><startdate>20161101</startdate><enddate>20161101</enddate><creator>Al-Hazimi, Mansour A</creator><creator>Somma, M N</creator><creator>Waggas, A S</creator><creator>Rawi, Sayed M</creator><general>Triveni Enterprises</general><scope>AAYXX</scope><scope>CITATION</scope><scope>04Q</scope><scope>04W</scope><scope>3V.</scope><scope>7ST</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>SOI</scope><scope>7TK</scope></search><sort><creationdate>20161101</creationdate><title>Neurotoxicological characterization of fractionated polypeptide from the Sea Anemone Gyrostoma helianthus on male albino rat</title><author>Al-Hazimi, Mansour A ; Somma, M N ; Waggas, A S ; Rawi, Sayed M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2160-4b1e94cffa91658bbf37ee49bceca83812e4585e1287431d03c03f40dd0f11f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Edema</topic><topic>Environmental science</topic><topic>Gyrostoma</topic><topic>Helianthus</topic><topic>Light microscopy</topic><topic>Marine organisms</topic><topic>Muscles</topic><topic>Neurotoxicity</topic><topic>Toxins</topic><toplevel>online_resources</toplevel><creatorcontrib>Al-Hazimi, Mansour A</creatorcontrib><creatorcontrib>Somma, M N</creatorcontrib><creatorcontrib>Waggas, A S</creatorcontrib><creatorcontrib>Rawi, Sayed M</creatorcontrib><creatorcontrib>King Abdulaziz University</creatorcontrib><collection>CrossRef</collection><collection>India Database</collection><collection>India Database: Science &amp; Technology</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Toxicology Abstracts</collection><collection>ProQuest_Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Environment Abstracts</collection><collection>Neurosciences Abstracts</collection><jtitle>Journal of environmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Al-Hazimi, Mansour A</au><au>Somma, M N</au><au>Waggas, A S</au><au>Rawi, Sayed M</au><aucorp>King Abdulaziz University</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neurotoxicological characterization of fractionated polypeptide from the Sea Anemone Gyrostoma helianthus on male albino rat</atitle><jtitle>Journal of environmental biology</jtitle><date>2016-11-01</date><risdate>2016</risdate><volume>37</volume><issue>6</issue><spage>1385</spage><epage>1391</epage><pages>1385-1391</pages><issn>0254-8704</issn><eissn>2394-0379</eissn><abstract>Marine organisms represent a valuable source of new compounds. The present study focused on the neurotoxicity of ethanolic crude extract and partially purified protein of Anemone species collected from the Red Sea shore of Jeddah, Saudia Arabia. The ethanolic extracts were found toxic to rats and the median lethal dose (LD50) was 20.3 mg kg b. wt. after IP of the crude extract. The rats showed behavioral changes suchas tonic convulsions, paralysis and flexing of muscles. A single dose equal to ¼ LD50 administered IP, the crude extract induced differential and time-dependentdopamine (DA), norepinephrine (NE), and serotonin (5-HT)neuronal neurotransmitter changes; reduced glutathione level(P&lt; 0.01)and highly affected cerebral histopathological disturbances. The crude extract and peptide fraction of 1KDa showed highest norepinephrine (P &lt;0.01) and dopamine (P&lt;0.01) stimulatory activities. Whereas protein fraction of 500Da resulted in maximal increase (P &lt;0.01) in serotonin post 30 and 60 min. In addition, the studied toxins vigorously affected rat brain cerebral cortex histological structure, the crude extract as well as the protein fraction of 1KDa disclosed the most histopathological effects. Light microscopy revealed neural hemorrhage, formation of pycnotic nuclei, marked edema due to enlargement of Virchow, congestion of blood vessels and focal hemorrhage.</abstract><cop>Lucknow</cop><pub>Triveni Enterprises</pub><doi>10.22438/jeb/37/6/mrn-280</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0254-8704
ispartof Journal of environmental biology, 2016-11, Vol.37 (6), p.1385-1391
issn 0254-8704
2394-0379
language eng
recordid cdi_proquest_miscellaneous_1877817847
source EZB Electronic Journals Library
subjects Edema
Environmental science
Gyrostoma
Helianthus
Light microscopy
Marine organisms
Muscles
Neurotoxicity
Toxins
title Neurotoxicological characterization of fractionated polypeptide from the Sea Anemone Gyrostoma helianthus on male albino rat
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T07%3A49%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Neurotoxicological%20characterization%20of%20fractionated%20polypeptide%20from%20the%20Sea%20Anemone%20Gyrostoma%20helianthus%20on%20male%20albino%20rat&rft.jtitle=Journal%20of%20environmental%20biology&rft.au=Al-Hazimi,%20Mansour%20A&rft.aucorp=King%20Abdulaziz%20University&rft.date=2016-11-01&rft.volume=37&rft.issue=6&rft.spage=1385&rft.epage=1391&rft.pages=1385-1391&rft.issn=0254-8704&rft.eissn=2394-0379&rft_id=info:doi/10.22438/jeb/37/6/mrn-280&rft_dat=%3Cproquest_cross%3E4313935601%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1868286745&rft_id=info:pmid/&rfr_iscdi=true