Locomotion Activity Meter for Quality Assessment of Mass-Reared Sterile Male Moths (Lepidoptera)
Irradiation is used to provide sterile insects from mass-rearing facilities, but irradiation can degrade insect quality. A system is described that uses repeatable pheromone stimuli to activate male moths housed with clean airflow in a commercially available insect activity meter, for potential use...
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| Veröffentlicht in: | The Florida entomologist 2016-06, Vol.99 (sp1), p.131-137 |
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| creator | Brown, Robert L Stanbury, Mailee El-Sayed, Ashraf M Laban, John Butler, Ruth Suckling, David M |
| description | Irradiation is used to provide sterile insects from mass-rearing facilities, but irradiation can degrade insect quality. A system is described that uses repeatable pheromone stimuli to activate male moths housed with clean airflow in a commercially available insect activity meter, for potential use in quality assessment of mass-reared moths in sterile insect programs. We tested sexually mature wild and sterile light brown apple moth (LBAM), Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae), males at the commencement of scotophase in a simple assay for increased mate-seeking activity after a 2 s stimulus with a 4-component synthetic pheromone source for 2 s, after a 5 s baseline of activity. Male activity at the upwind end of the glass tube was assessed by infrared beam. Next, we tested if a temperature shock at 30 °C in the pupal stage for various durations (0, 1, 2 or 4 h) would have an effect on male moth response to the sex pheromone. The results indicated similar baseline activity in clean airflow, but a significantly greater response after pheromone stimulation from non-irradiated males, compared with irradiated males. Responses from irradiated males averaged 78% of the control response (n = 320). The temperature shock did not appear to change the response of the non-irradiated moths (n = 64), but there was a slight decline in response by irradiated moths at the 2 and 4 h temperature shock durations. The system could be readily modified to be suitable for factory scale quality assurance. |
| doi_str_mv | 10.1653/024.099.sp116 |
| format | Article |
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A system is described that uses repeatable pheromone stimuli to activate male moths housed with clean airflow in a commercially available insect activity meter, for potential use in quality assessment of mass-reared moths in sterile insect programs. We tested sexually mature wild and sterile light brown apple moth (LBAM), Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae), males at the commencement of scotophase in a simple assay for increased mate-seeking activity after a 2 s stimulus with a 4-component synthetic pheromone source for 2 s, after a 5 s baseline of activity. Male activity at the upwind end of the glass tube was assessed by infrared beam. Next, we tested if a temperature shock at 30 °C in the pupal stage for various durations (0, 1, 2 or 4 h) would have an effect on male moth response to the sex pheromone. The results indicated similar baseline activity in clean airflow, but a significantly greater response after pheromone stimulation from non-irradiated males, compared with irradiated males. Responses from irradiated males averaged 78% of the control response (n = 320). The temperature shock did not appear to change the response of the non-irradiated moths (n = 64), but there was a slight decline in response by irradiated moths at the 2 and 4 h temperature shock durations. The system could be readily modified to be suitable for factory scale quality assurance.</description><identifier>ISSN: 0015-4040</identifier><identifier>EISSN: 1938-5102</identifier><identifier>DOI: 10.1653/024.099.sp116</identifier><identifier>CODEN: FETMAC</identifier><language>eng</language><publisher>Lutz: Florida Entomological Society</publisher><subject>aptitud ; Behavior ; Butterflies & moths ; competitiveness ; competitividad ; Epiphyas postvittana ; Experiments ; fitness ; Fruits ; Insect sterilization ; Insects ; irradiación ; irradiation ; la palomilla marrón de la manzana ; Laboratories ; Lepidoptera ; Light brown apple moth ; Males ; Malus ; Methods ; Pheromones ; Physiological aspects ; Quality ; sterile insect technique ; Studies ; Temperature ; TOOLS AND METHODS TO ASSESS FIELD PERFORMANCE ; Tortricidae ; técnica del insecto estéril</subject><ispartof>The Florida entomologist, 2016-06, Vol.99 (sp1), p.131-137</ispartof><rights>International Atomic Energy Agency 2016. Published by the Florida Entomological Society. All rights reserved.</rights><rights>COPYRIGHT 2016 Florida Entomological Society</rights><rights>Copyright Florida Entomological Society Jun 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b446t-1f48ea07747f08fb6f63ebc00d65f6ce46545a6e31e2084ce14ac198893a2e983</citedby><cites>FETCH-LOGICAL-b446t-1f48ea07747f08fb6f63ebc00d65f6ce46545a6e31e2084ce14ac198893a2e983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1653/024.099.sp116$$EPDF$$P50$$Gbioone$$H</linktopdf><link.rule.ids>109,314,776,780,860,27903,27904,52698</link.rule.ids></links><search><creatorcontrib>Brown, Robert L</creatorcontrib><creatorcontrib>Stanbury, Mailee</creatorcontrib><creatorcontrib>El-Sayed, Ashraf M</creatorcontrib><creatorcontrib>Laban, John</creatorcontrib><creatorcontrib>Butler, Ruth</creatorcontrib><creatorcontrib>Suckling, David M</creatorcontrib><title>Locomotion Activity Meter for Quality Assessment of Mass-Reared Sterile Male Moths (Lepidoptera)</title><title>The Florida entomologist</title><description>Irradiation is used to provide sterile insects from mass-rearing facilities, but irradiation can degrade insect quality. A system is described that uses repeatable pheromone stimuli to activate male moths housed with clean airflow in a commercially available insect activity meter, for potential use in quality assessment of mass-reared moths in sterile insect programs. We tested sexually mature wild and sterile light brown apple moth (LBAM), Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae), males at the commencement of scotophase in a simple assay for increased mate-seeking activity after a 2 s stimulus with a 4-component synthetic pheromone source for 2 s, after a 5 s baseline of activity. Male activity at the upwind end of the glass tube was assessed by infrared beam. Next, we tested if a temperature shock at 30 °C in the pupal stage for various durations (0, 1, 2 or 4 h) would have an effect on male moth response to the sex pheromone. The results indicated similar baseline activity in clean airflow, but a significantly greater response after pheromone stimulation from non-irradiated males, compared with irradiated males. Responses from irradiated males averaged 78% of the control response (n = 320). The temperature shock did not appear to change the response of the non-irradiated moths (n = 64), but there was a slight decline in response by irradiated moths at the 2 and 4 h temperature shock durations. The system could be readily modified to be suitable for factory scale quality assurance.</description><subject>aptitud</subject><subject>Behavior</subject><subject>Butterflies & moths</subject><subject>competitiveness</subject><subject>competitividad</subject><subject>Epiphyas postvittana</subject><subject>Experiments</subject><subject>fitness</subject><subject>Fruits</subject><subject>Insect sterilization</subject><subject>Insects</subject><subject>irradiación</subject><subject>irradiation</subject><subject>la palomilla marrón de la manzana</subject><subject>Laboratories</subject><subject>Lepidoptera</subject><subject>Light brown apple moth</subject><subject>Males</subject><subject>Malus</subject><subject>Methods</subject><subject>Pheromones</subject><subject>Physiological aspects</subject><subject>Quality</subject><subject>sterile insect technique</subject><subject>Studies</subject><subject>Temperature</subject><subject>TOOLS AND METHODS TO ASSESS FIELD PERFORMANCE</subject><subject>Tortricidae</subject><subject>técnica del insecto 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Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brown, Robert L</au><au>Stanbury, Mailee</au><au>El-Sayed, Ashraf M</au><au>Laban, John</au><au>Butler, Ruth</au><au>Suckling, David M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Locomotion Activity Meter for Quality Assessment of Mass-Reared Sterile Male Moths (Lepidoptera)</atitle><jtitle>The Florida entomologist</jtitle><date>2016-06</date><risdate>2016</risdate><volume>99</volume><issue>sp1</issue><spage>131</spage><epage>137</epage><pages>131-137</pages><issn>0015-4040</issn><eissn>1938-5102</eissn><coden>FETMAC</coden><abstract>Irradiation is used to provide sterile insects from mass-rearing facilities, but irradiation can degrade insect quality. A system is described that uses repeatable pheromone stimuli to activate male moths housed with clean airflow in a commercially available insect activity meter, for potential use in quality assessment of mass-reared moths in sterile insect programs. We tested sexually mature wild and sterile light brown apple moth (LBAM), Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae), males at the commencement of scotophase in a simple assay for increased mate-seeking activity after a 2 s stimulus with a 4-component synthetic pheromone source for 2 s, after a 5 s baseline of activity. Male activity at the upwind end of the glass tube was assessed by infrared beam. Next, we tested if a temperature shock at 30 °C in the pupal stage for various durations (0, 1, 2 or 4 h) would have an effect on male moth response to the sex pheromone. The results indicated similar baseline activity in clean airflow, but a significantly greater response after pheromone stimulation from non-irradiated males, compared with irradiated males. Responses from irradiated males averaged 78% of the control response (n = 320). The temperature shock did not appear to change the response of the non-irradiated moths (n = 64), but there was a slight decline in response by irradiated moths at the 2 and 4 h temperature shock durations. The system could be readily modified to be suitable for factory scale quality assurance.</abstract><cop>Lutz</cop><pub>Florida Entomological Society</pub><doi>10.1653/024.099.sp116</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; BioOne Open Access Titles; Jstor Journals Open Access; EZB-FREE-00999 freely available EZB journals |
| subjects | aptitud Behavior Butterflies & moths competitiveness competitividad Epiphyas postvittana Experiments fitness Fruits Insect sterilization Insects irradiación irradiation la palomilla marrón de la manzana Laboratories Lepidoptera Light brown apple moth Males Malus Methods Pheromones Physiological aspects Quality sterile insect technique Studies Temperature TOOLS AND METHODS TO ASSESS FIELD PERFORMANCE Tortricidae técnica del insecto estéril |
| title | Locomotion Activity Meter for Quality Assessment of Mass-Reared Sterile Male Moths (Lepidoptera) |
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