Silencing of hTERT blocks growth and migration of anaplastic thyroid cancer cells
Mutations in the hTERT promoter responsible for constitutive telomerase activity are the most frequent genetic alteration detected in anaplastic thyroid cancer (ATC), and proposed as diagnostic and prognostic biomarker in these tumours. In this study we analyzed hTERT expression in a series of human...
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| Veröffentlicht in: | Molecular and cellular endocrinology 2017-06, Vol.448, p.34-40 |
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| creator | Maggisano, Valentina Celano, Marilena Lombardo, Giovanni Enrico Lepore, Saverio Massimo Sponziello, Marialuisa Rosignolo, Francesca Verrienti, Antonella Baldan, Federica Puxeddu, Efisio Durante, Cosimo Filetti, Sebastiano Damante, Giuseppe Russo, Diego Bulotta, Stefania |
| description | Mutations in the hTERT promoter responsible for constitutive telomerase activity are the most frequent genetic alteration detected in anaplastic thyroid cancer (ATC), and proposed as diagnostic and prognostic biomarker in these tumours. In this study we analyzed hTERT expression in a series of human ATCs and investigated the effects of small-interfering RNA-mediated silencing of hTERT on viability and migration and invasive properties of three human ATC cell lines. Expression of hTERT mRNA resulted increased in 8/10 ATCs compared to normal thyroid tissues. Silencing of hTERT in CAL-62, 8505C and SW1736 cells did not modify telomere length but determined a significant decrease (about 50%) of cell proliferation in all cell lines and a great reduction (about 50%) of migration and invasion capacity. These finding demonstrate that hTERT may be considered as a molecular target for ATC treatment.
•Overexpression of hTERT has been detected in ATCs.•TERT silencing blocks proliferation of two ATC cell lines.•TERT silencing reduces migration and invasion of ATC cells.•hTERT as new molecular target for ATC treatment. |
| doi_str_mv | 10.1016/j.mce.2017.03.007 |
| format | Article |
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•Overexpression of hTERT has been detected in ATCs.•TERT silencing blocks proliferation of two ATC cell lines.•TERT silencing reduces migration and invasion of ATC cells.•hTERT as new molecular target for ATC treatment.</description><identifier>ISSN: 0303-7207</identifier><identifier>EISSN: 1872-8057</identifier><identifier>DOI: 10.1016/j.mce.2017.03.007</identifier><identifier>PMID: 28288903</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Cell Cycle ; Cell Movement - genetics ; Cell Proliferation - genetics ; Cell Survival ; Female ; Gene Expression Regulation, Neoplastic ; Gene Silencing ; Growth inhibition ; Humans ; Invasion ; Male ; Middle Aged ; Migration ; Neoplasm Invasiveness ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; RNA, Small Interfering - metabolism ; siRNA ; Telomerase ; Telomerase - genetics ; Telomerase - metabolism ; Telomere - metabolism ; Thyroid cancer ; Thyroid Carcinoma, Anaplastic - enzymology ; Thyroid Carcinoma, Anaplastic - genetics ; Thyroid Carcinoma, Anaplastic - pathology</subject><ispartof>Molecular and cellular endocrinology, 2017-06, Vol.448, p.34-40</ispartof><rights>2017 Elsevier B.V.</rights><rights>Copyright © 2017 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-8d66b66ec0e55fa08355e0af64086961251d749ec54ae6ad54ef87c1fe071c763</citedby><cites>FETCH-LOGICAL-c396t-8d66b66ec0e55fa08355e0af64086961251d749ec54ae6ad54ef87c1fe071c763</cites><orcidid>0000-0003-1164-1316</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mce.2017.03.007$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28288903$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maggisano, Valentina</creatorcontrib><creatorcontrib>Celano, Marilena</creatorcontrib><creatorcontrib>Lombardo, Giovanni Enrico</creatorcontrib><creatorcontrib>Lepore, Saverio Massimo</creatorcontrib><creatorcontrib>Sponziello, Marialuisa</creatorcontrib><creatorcontrib>Rosignolo, Francesca</creatorcontrib><creatorcontrib>Verrienti, Antonella</creatorcontrib><creatorcontrib>Baldan, Federica</creatorcontrib><creatorcontrib>Puxeddu, Efisio</creatorcontrib><creatorcontrib>Durante, Cosimo</creatorcontrib><creatorcontrib>Filetti, Sebastiano</creatorcontrib><creatorcontrib>Damante, Giuseppe</creatorcontrib><creatorcontrib>Russo, Diego</creatorcontrib><creatorcontrib>Bulotta, Stefania</creatorcontrib><title>Silencing of hTERT blocks growth and migration of anaplastic thyroid cancer cells</title><title>Molecular and cellular endocrinology</title><addtitle>Mol Cell Endocrinol</addtitle><description>Mutations in the hTERT promoter responsible for constitutive telomerase activity are the most frequent genetic alteration detected in anaplastic thyroid cancer (ATC), and proposed as diagnostic and prognostic biomarker in these tumours. In this study we analyzed hTERT expression in a series of human ATCs and investigated the effects of small-interfering RNA-mediated silencing of hTERT on viability and migration and invasive properties of three human ATC cell lines. Expression of hTERT mRNA resulted increased in 8/10 ATCs compared to normal thyroid tissues. Silencing of hTERT in CAL-62, 8505C and SW1736 cells did not modify telomere length but determined a significant decrease (about 50%) of cell proliferation in all cell lines and a great reduction (about 50%) of migration and invasion capacity. These finding demonstrate that hTERT may be considered as a molecular target for ATC treatment.
•Overexpression of hTERT has been detected in ATCs.•TERT silencing blocks proliferation of two ATC cell lines.•TERT silencing reduces migration and invasion of ATC cells.•hTERT as new molecular target for ATC treatment.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Cell Cycle</subject><subject>Cell Movement - genetics</subject><subject>Cell Proliferation - genetics</subject><subject>Cell Survival</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gene Silencing</subject><subject>Growth inhibition</subject><subject>Humans</subject><subject>Invasion</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Migration</subject><subject>Neoplasm Invasiveness</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Small Interfering - metabolism</subject><subject>siRNA</subject><subject>Telomerase</subject><subject>Telomerase - genetics</subject><subject>Telomerase - metabolism</subject><subject>Telomere - metabolism</subject><subject>Thyroid cancer</subject><subject>Thyroid Carcinoma, Anaplastic - enzymology</subject><subject>Thyroid Carcinoma, Anaplastic - genetics</subject><subject>Thyroid Carcinoma, Anaplastic - pathology</subject><issn>0303-7207</issn><issn>1872-8057</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1v2zAURYmgRew6-QFZCo5dpD5K5ofQqTCcpICBoo0zEzT1ZNOVRJeUE_jfh4Ldjpnecu7Fu4eQOwY5Aya-7vPOYl4AkzmUOYC8IlOmZJEp4PIDmUIJZSYLkBPyKcY9JIIX6ppMClUoVUE5Jb-eXIu9df2W-obu1svfa7ppvf0T6Tb412FHTV_Tzm2DGZzvR8j05tCaODhLh90peFdTa3qLgVps23hDPjamjXh7uTPyfL9cLx6z1c-HH4vvq8yWlRgyVQuxEQItIOeNAVVyjmAaMQclKsEKzmo5r9DyuUFhaj7HRknLGgTJrBTljHw59x6C_3vEOOjOxfED06M_Rp1EpLW8ghFlZ9QGH2PARh-C60w4aQZ6NKn3OpnUo0kNpU6eUubzpf646bD-n_inLgHfzgCmkS8Og47WJZVYu4B20LV379S_AbOAg1c</recordid><startdate>20170615</startdate><enddate>20170615</enddate><creator>Maggisano, Valentina</creator><creator>Celano, Marilena</creator><creator>Lombardo, Giovanni Enrico</creator><creator>Lepore, Saverio Massimo</creator><creator>Sponziello, Marialuisa</creator><creator>Rosignolo, Francesca</creator><creator>Verrienti, Antonella</creator><creator>Baldan, Federica</creator><creator>Puxeddu, Efisio</creator><creator>Durante, Cosimo</creator><creator>Filetti, Sebastiano</creator><creator>Damante, Giuseppe</creator><creator>Russo, Diego</creator><creator>Bulotta, Stefania</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1164-1316</orcidid></search><sort><creationdate>20170615</creationdate><title>Silencing of hTERT blocks growth and migration of anaplastic thyroid cancer cells</title><author>Maggisano, Valentina ; Celano, Marilena ; Lombardo, Giovanni Enrico ; Lepore, Saverio Massimo ; Sponziello, Marialuisa ; Rosignolo, Francesca ; Verrienti, Antonella ; Baldan, Federica ; Puxeddu, Efisio ; Durante, Cosimo ; Filetti, Sebastiano ; Damante, Giuseppe ; Russo, Diego ; Bulotta, Stefania</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-8d66b66ec0e55fa08355e0af64086961251d749ec54ae6ad54ef87c1fe071c763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Cell Cycle</topic><topic>Cell Movement - genetics</topic><topic>Cell Proliferation - genetics</topic><topic>Cell Survival</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Gene Silencing</topic><topic>Growth inhibition</topic><topic>Humans</topic><topic>Invasion</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Migration</topic><topic>Neoplasm Invasiveness</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA, Small Interfering - metabolism</topic><topic>siRNA</topic><topic>Telomerase</topic><topic>Telomerase - genetics</topic><topic>Telomerase - metabolism</topic><topic>Telomere - metabolism</topic><topic>Thyroid cancer</topic><topic>Thyroid Carcinoma, Anaplastic - enzymology</topic><topic>Thyroid Carcinoma, Anaplastic - genetics</topic><topic>Thyroid Carcinoma, Anaplastic - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maggisano, Valentina</creatorcontrib><creatorcontrib>Celano, Marilena</creatorcontrib><creatorcontrib>Lombardo, Giovanni Enrico</creatorcontrib><creatorcontrib>Lepore, Saverio Massimo</creatorcontrib><creatorcontrib>Sponziello, Marialuisa</creatorcontrib><creatorcontrib>Rosignolo, Francesca</creatorcontrib><creatorcontrib>Verrienti, Antonella</creatorcontrib><creatorcontrib>Baldan, Federica</creatorcontrib><creatorcontrib>Puxeddu, Efisio</creatorcontrib><creatorcontrib>Durante, Cosimo</creatorcontrib><creatorcontrib>Filetti, Sebastiano</creatorcontrib><creatorcontrib>Damante, Giuseppe</creatorcontrib><creatorcontrib>Russo, Diego</creatorcontrib><creatorcontrib>Bulotta, Stefania</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maggisano, Valentina</au><au>Celano, Marilena</au><au>Lombardo, Giovanni Enrico</au><au>Lepore, Saverio Massimo</au><au>Sponziello, Marialuisa</au><au>Rosignolo, Francesca</au><au>Verrienti, Antonella</au><au>Baldan, Federica</au><au>Puxeddu, Efisio</au><au>Durante, Cosimo</au><au>Filetti, Sebastiano</au><au>Damante, Giuseppe</au><au>Russo, Diego</au><au>Bulotta, Stefania</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silencing of hTERT blocks growth and migration of anaplastic thyroid cancer cells</atitle><jtitle>Molecular and cellular endocrinology</jtitle><addtitle>Mol Cell Endocrinol</addtitle><date>2017-06-15</date><risdate>2017</risdate><volume>448</volume><spage>34</spage><epage>40</epage><pages>34-40</pages><issn>0303-7207</issn><eissn>1872-8057</eissn><abstract>Mutations in the hTERT promoter responsible for constitutive telomerase activity are the most frequent genetic alteration detected in anaplastic thyroid cancer (ATC), and proposed as diagnostic and prognostic biomarker in these tumours. In this study we analyzed hTERT expression in a series of human ATCs and investigated the effects of small-interfering RNA-mediated silencing of hTERT on viability and migration and invasive properties of three human ATC cell lines. Expression of hTERT mRNA resulted increased in 8/10 ATCs compared to normal thyroid tissues. Silencing of hTERT in CAL-62, 8505C and SW1736 cells did not modify telomere length but determined a significant decrease (about 50%) of cell proliferation in all cell lines and a great reduction (about 50%) of migration and invasion capacity. These finding demonstrate that hTERT may be considered as a molecular target for ATC treatment.
•Overexpression of hTERT has been detected in ATCs.•TERT silencing blocks proliferation of two ATC cell lines.•TERT silencing reduces migration and invasion of ATC cells.•hTERT as new molecular target for ATC treatment.</abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>28288903</pmid><doi>10.1016/j.mce.2017.03.007</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-1164-1316</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Aged Aged, 80 and over Cell Cycle Cell Movement - genetics Cell Proliferation - genetics Cell Survival Female Gene Expression Regulation, Neoplastic Gene Silencing Growth inhibition Humans Invasion Male Middle Aged Migration Neoplasm Invasiveness RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Small Interfering - metabolism siRNA Telomerase Telomerase - genetics Telomerase - metabolism Telomere - metabolism Thyroid cancer Thyroid Carcinoma, Anaplastic - enzymology Thyroid Carcinoma, Anaplastic - genetics Thyroid Carcinoma, Anaplastic - pathology |
| title | Silencing of hTERT blocks growth and migration of anaplastic thyroid cancer cells |
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