The added value of hepatitis E diagnostics in determining causes of hepatitis in routine diagnostic settings in the Netherlands
Hepatitis E virus (HEV) genotype 3 is endemic in Europe and an underdiagnosed and emerging (public) health issue. In recent years commercial enzyme immunoassays (EIAs) that detect antibodies to HEV more adequately, became available. We investigated the added value of this HEV serology in the diagnos...
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| Veröffentlicht in: | Clinical microbiology and infection 2017-09, Vol.23 (9), p.667-671 |
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| container_title | Clinical microbiology and infection |
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| creator | Doting, M.H.E. Weel, J. Niesters, H.G.M. Riezebos-Brilman, A. Brandenburg, A. |
| description | Hepatitis E virus (HEV) genotype 3 is endemic in Europe and an underdiagnosed and emerging (public) health issue. In recent years commercial enzyme immunoassays (EIAs) that detect antibodies to HEV more adequately, became available. We investigated the added value of this HEV serology in the diagnostic work flow to detect viral causes of recent hepatitis.
During a 2-year period (May 2013 to May 2015), HEV serology was added to the hepatitis work flow, consisting of serological detection of hepatitis viruses A, B and C (HAV, HBV, HCV), Epstein–Barr virus (EBV) and cytomegalovirus (CMV). Samples positive for HEV IgM were also analysed using PCR to detect HEV RNA. If positive, HEV sequencing was performed for genotyping purposes.
In 235 out of 2521 patients (9.3%), a viral cause for hepatitis was found. Recent HAV, HBV, HCV, EBV or CMV infections were serologically diagnosed in 3, 34, 10, 69 and 42 patients, respectively. Seventy-eight patients (3.1%) had a recent HEV infection. In 49 of them, sufficient HEV RNA was present for genotyping. All patients were infected with HEV genotype 3.
In our region, an HEV infection is the most frequently diagnosed viral cause for recent hepatitis. These results indicate that, in a country where HEV is endemic, serological HEV diagnostics should be added to the standard work-up for viral hepatitis. |
| doi_str_mv | 10.1016/j.cmi.2017.02.026 |
| format | Article |
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During a 2-year period (May 2013 to May 2015), HEV serology was added to the hepatitis work flow, consisting of serological detection of hepatitis viruses A, B and C (HAV, HBV, HCV), Epstein–Barr virus (EBV) and cytomegalovirus (CMV). Samples positive for HEV IgM were also analysed using PCR to detect HEV RNA. If positive, HEV sequencing was performed for genotyping purposes.
In 235 out of 2521 patients (9.3%), a viral cause for hepatitis was found. Recent HAV, HBV, HCV, EBV or CMV infections were serologically diagnosed in 3, 34, 10, 69 and 42 patients, respectively. Seventy-eight patients (3.1%) had a recent HEV infection. In 49 of them, sufficient HEV RNA was present for genotyping. All patients were infected with HEV genotype 3.
In our region, an HEV infection is the most frequently diagnosed viral cause for recent hepatitis. These results indicate that, in a country where HEV is endemic, serological HEV diagnostics should be added to the standard work-up for viral hepatitis.</description><identifier>ISSN: 1198-743X</identifier><identifier>EISSN: 1469-0691</identifier><identifier>DOI: 10.1016/j.cmi.2017.02.026</identifier><identifier>PMID: 28285979</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Adolescent ; Adult ; Aged ; Child ; Diagnostics ; Female ; General practitioner ; Genotyping ; Hepatitis E - diagnosis ; Hepatitis E - epidemiology ; Hepatitis E - virology ; Hepatitis E virus ; Hepatitis E virus - classification ; Hepatitis E virus - genetics ; Humans ; Male ; Middle Aged ; Molecular Diagnostic Techniques - methods ; Molecular Diagnostic Techniques - statistics & numerical data ; Molecular Typing - methods ; Molecular Typing - statistics & numerical data ; Netherlands - epidemiology ; Predictive Value of Tests ; Recent hepatitis ; Seroepidemiologic Studies ; Serology ; The Netherlands ; Viral ; Young Adult</subject><ispartof>Clinical microbiology and infection, 2017-09, Vol.23 (9), p.667-671</ispartof><rights>2017 European Society of Clinical Microbiology and Infectious Diseases</rights><rights>Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-fa2d13a957fd6baf39905c3d151715d0e5d6d83458e78dd6c80ed9885ed3899b3</citedby><cites>FETCH-LOGICAL-c396t-fa2d13a957fd6baf39905c3d151715d0e5d6d83458e78dd6c80ed9885ed3899b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28285979$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Doting, M.H.E.</creatorcontrib><creatorcontrib>Weel, J.</creatorcontrib><creatorcontrib>Niesters, H.G.M.</creatorcontrib><creatorcontrib>Riezebos-Brilman, A.</creatorcontrib><creatorcontrib>Brandenburg, A.</creatorcontrib><title>The added value of hepatitis E diagnostics in determining causes of hepatitis in routine diagnostic settings in the Netherlands</title><title>Clinical microbiology and infection</title><addtitle>Clin Microbiol Infect</addtitle><description>Hepatitis E virus (HEV) genotype 3 is endemic in Europe and an underdiagnosed and emerging (public) health issue. In recent years commercial enzyme immunoassays (EIAs) that detect antibodies to HEV more adequately, became available. We investigated the added value of this HEV serology in the diagnostic work flow to detect viral causes of recent hepatitis.
During a 2-year period (May 2013 to May 2015), HEV serology was added to the hepatitis work flow, consisting of serological detection of hepatitis viruses A, B and C (HAV, HBV, HCV), Epstein–Barr virus (EBV) and cytomegalovirus (CMV). Samples positive for HEV IgM were also analysed using PCR to detect HEV RNA. If positive, HEV sequencing was performed for genotyping purposes.
In 235 out of 2521 patients (9.3%), a viral cause for hepatitis was found. Recent HAV, HBV, HCV, EBV or CMV infections were serologically diagnosed in 3, 34, 10, 69 and 42 patients, respectively. Seventy-eight patients (3.1%) had a recent HEV infection. In 49 of them, sufficient HEV RNA was present for genotyping. All patients were infected with HEV genotype 3.
In our region, an HEV infection is the most frequently diagnosed viral cause for recent hepatitis. These results indicate that, in a country where HEV is endemic, serological HEV diagnostics should be added to the standard work-up for viral hepatitis.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Child</subject><subject>Diagnostics</subject><subject>Female</subject><subject>General practitioner</subject><subject>Genotyping</subject><subject>Hepatitis E - diagnosis</subject><subject>Hepatitis E - epidemiology</subject><subject>Hepatitis E - virology</subject><subject>Hepatitis E virus</subject><subject>Hepatitis E virus - classification</subject><subject>Hepatitis E virus - genetics</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Molecular Diagnostic Techniques - methods</subject><subject>Molecular Diagnostic Techniques - statistics & numerical data</subject><subject>Molecular Typing - methods</subject><subject>Molecular Typing - statistics & numerical data</subject><subject>Netherlands - epidemiology</subject><subject>Predictive Value of Tests</subject><subject>Recent hepatitis</subject><subject>Seroepidemiologic Studies</subject><subject>Serology</subject><subject>The Netherlands</subject><subject>Viral</subject><subject>Young Adult</subject><issn>1198-743X</issn><issn>1469-0691</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFOGzEQhq2qqEmhD9BL5WMvG-x17LXFCUVAK6FyAYmb5XhmE0fZ3WB7kbj1UfosPFkNoYUT0si2xv_3e_wT8pWzGWdcHW9mvguzmvFmxupS6gOZ8rkyFVOGfyxnbnTVzMXthHxOacMYq4WYfyKTWtdamsZMye_rNVIHgEDv3XZEOrR0jTuXQw6JnlEIbtUPKQefaOgpYMbYhT70K-rdmDAV4PHPK1E0cRhz6PENShPm0lo9X-fy4C8sa9y6HtIROWjdNuGXl_2Q3JyfXS9-VJdXFz8Xp5eVF0blqnU1cOGMbFpQS9cKY5j0ArjkDZfAUIICLeZSY6MBlNcMwWgtEYQ2ZikOyfe97y4OdyOmbLuQPG7LEDiMyXLdKM0bI0WR8r3UxyGliK3dxdC5-GA5s0_B240twdun4C2rS6nCfHuxH5cdwn_iX9JFcLIXYPnkfcBokw_Ye4QQ0WcLQ3jH_i8tz5bT</recordid><startdate>201709</startdate><enddate>201709</enddate><creator>Doting, M.H.E.</creator><creator>Weel, J.</creator><creator>Niesters, H.G.M.</creator><creator>Riezebos-Brilman, A.</creator><creator>Brandenburg, A.</creator><general>Elsevier Ltd</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201709</creationdate><title>The added value of hepatitis E diagnostics in determining causes of hepatitis in routine diagnostic settings in the Netherlands</title><author>Doting, M.H.E. ; Weel, J. ; Niesters, H.G.M. ; Riezebos-Brilman, A. ; Brandenburg, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-fa2d13a957fd6baf39905c3d151715d0e5d6d83458e78dd6c80ed9885ed3899b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Child</topic><topic>Diagnostics</topic><topic>Female</topic><topic>General practitioner</topic><topic>Genotyping</topic><topic>Hepatitis E - diagnosis</topic><topic>Hepatitis E - epidemiology</topic><topic>Hepatitis E - virology</topic><topic>Hepatitis E virus</topic><topic>Hepatitis E virus - classification</topic><topic>Hepatitis E virus - genetics</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Molecular Diagnostic Techniques - methods</topic><topic>Molecular Diagnostic Techniques - statistics & numerical data</topic><topic>Molecular Typing - methods</topic><topic>Molecular Typing - statistics & numerical data</topic><topic>Netherlands - epidemiology</topic><topic>Predictive Value of Tests</topic><topic>Recent hepatitis</topic><topic>Seroepidemiologic Studies</topic><topic>Serology</topic><topic>The Netherlands</topic><topic>Viral</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Doting, M.H.E.</creatorcontrib><creatorcontrib>Weel, J.</creatorcontrib><creatorcontrib>Niesters, H.G.M.</creatorcontrib><creatorcontrib>Riezebos-Brilman, A.</creatorcontrib><creatorcontrib>Brandenburg, A.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical microbiology and infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Doting, M.H.E.</au><au>Weel, J.</au><au>Niesters, H.G.M.</au><au>Riezebos-Brilman, A.</au><au>Brandenburg, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The added value of hepatitis E diagnostics in determining causes of hepatitis in routine diagnostic settings in the Netherlands</atitle><jtitle>Clinical microbiology and infection</jtitle><addtitle>Clin Microbiol Infect</addtitle><date>2017-09</date><risdate>2017</risdate><volume>23</volume><issue>9</issue><spage>667</spage><epage>671</epage><pages>667-671</pages><issn>1198-743X</issn><eissn>1469-0691</eissn><abstract>Hepatitis E virus (HEV) genotype 3 is endemic in Europe and an underdiagnosed and emerging (public) health issue. In recent years commercial enzyme immunoassays (EIAs) that detect antibodies to HEV more adequately, became available. We investigated the added value of this HEV serology in the diagnostic work flow to detect viral causes of recent hepatitis.
During a 2-year period (May 2013 to May 2015), HEV serology was added to the hepatitis work flow, consisting of serological detection of hepatitis viruses A, B and C (HAV, HBV, HCV), Epstein–Barr virus (EBV) and cytomegalovirus (CMV). Samples positive for HEV IgM were also analysed using PCR to detect HEV RNA. If positive, HEV sequencing was performed for genotyping purposes.
In 235 out of 2521 patients (9.3%), a viral cause for hepatitis was found. Recent HAV, HBV, HCV, EBV or CMV infections were serologically diagnosed in 3, 34, 10, 69 and 42 patients, respectively. Seventy-eight patients (3.1%) had a recent HEV infection. In 49 of them, sufficient HEV RNA was present for genotyping. All patients were infected with HEV genotype 3.
In our region, an HEV infection is the most frequently diagnosed viral cause for recent hepatitis. These results indicate that, in a country where HEV is endemic, serological HEV diagnostics should be added to the standard work-up for viral hepatitis.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>28285979</pmid><doi>10.1016/j.cmi.2017.02.026</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Clinical microbiology and infection, 2017-09, Vol.23 (9), p.667-671 |
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| subjects | Adolescent Adult Aged Child Diagnostics Female General practitioner Genotyping Hepatitis E - diagnosis Hepatitis E - epidemiology Hepatitis E - virology Hepatitis E virus Hepatitis E virus - classification Hepatitis E virus - genetics Humans Male Middle Aged Molecular Diagnostic Techniques - methods Molecular Diagnostic Techniques - statistics & numerical data Molecular Typing - methods Molecular Typing - statistics & numerical data Netherlands - epidemiology Predictive Value of Tests Recent hepatitis Seroepidemiologic Studies Serology The Netherlands Viral Young Adult |
| title | The added value of hepatitis E diagnostics in determining causes of hepatitis in routine diagnostic settings in the Netherlands |
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