In-vitro culture of individual mouse preimplantation embryos: the role of embryo density, microwells, oxygen, timing and conditioned media
Highlights • Individual culture of preimplantation mouse embryos is detrimental during both the precompaction and postcompaction stages, demonstrated by reduced hatching rate and blastocyst cell numbers. • The effect of embryo density on singly cultured embryos is dependent on the incubator oxygen c...
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| Veröffentlicht in: | Reproductive biomedicine online 2017-05, Vol.34 (5), p.441-454 |
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| container_title | Reproductive biomedicine online |
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| creator | Kelley, Rebecca L Gardner, David K |
| description | Highlights • Individual culture of preimplantation mouse embryos is detrimental during both the precompaction and postcompaction stages, demonstrated by reduced hatching rate and blastocyst cell numbers. • The effect of embryo density on singly cultured embryos is dependent on the incubator oxygen concentration. • Culture in microwell dishes increased the percentage of inner cell mass per blastocyst, and altered timing of developmental events. • Addition of embryo-conditioned media to single embryos improves hatching rates and cell numbers. |
| doi_str_mv | 10.1016/j.rbmo.2017.02.001 |
| format | Article |
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| ispartof | Reproductive biomedicine online, 2017-05, Vol.34 (5), p.441-454 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Blastocyst Culture Media, Conditioned Embryo Culture Techniques - methods Female Group culture In Vitro Techniques IVF Mice Mice, Inbred C57BL Mice, Inbred CBA Obstetrics and Gynecology Osmolality Oxygen - metabolism Single culture Stress Time-lapse |
| title | In-vitro culture of individual mouse preimplantation embryos: the role of embryo density, microwells, oxygen, timing and conditioned media |
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