Optimizing cell‐free protein expression in CHO: Assessing small molecule mass transfer effects in various reactor configurations

ABSTRACT Cell‐free protein synthesis (CFPS) is an ideal platform for rapid and convenient protein production. However, bioreactor design remains a critical consideration in optimizing protein expression. Using turbo green fluorescent protein (tGFP) as a model, we tracked small molecule components in...

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Veröffentlicht in:	Biotechnology and bioengineering 2017-07, Vol.114 (7), p.1478-1486
Hauptverfasser:	Peñalber‐Johnstone, Chariz, Ge, Xudong, Tran, Kevin, Selock, Nicholas, Sardesai, Neha, Gurramkonda, Chandrasekhar, Pilli, Manohar, Tolosa, Michael, Tolosa, Leah, Kostov, Yordan, Frey, Douglas D., Rao, Govind
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bioengineering Bioprocessing Bioreactors Biotechnology Cell-Free System - metabolism Cellulose cell‐free protein synthesis Chemical synthesis Chinese hamster ovary cells CHO Cells - chemistry CHO Cells - metabolism Computing time continuous‐exchange cell‐free Creatine Cricetulus Design optimization Dialysis Fluorescence Format Green fluorescent protein in vitro protein expression Mass transfer Microdialysis Nucleoside triphosphates Phosphates Phosphocreatine Protein biosynthesis Protein Biosynthesis - physiology Protein Engineering - instrumentation Protein Engineering - methods Protein expression Protein synthesis Quality Control Yield
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