Large-scale production of recombinant Saw1 in Escherichia coli

Saccharomyces cerevisiae Saw1 is an essential gene in single-strand annealing – the DNA repair pathway that repairs double-strand breaks when they occur between homologous repeats. Saw1 interacts with the structure-specific nuclease Rad1-Rad10 and this results in the recruitment of this nuclease to...

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Veröffentlicht in:	Protein expression and purification 2017-05, Vol.133, p.75-80
Hauptverfasser:	Rashev, Margarita, Surtees, Jennifer A., Guarné, Alba
Format:	Artikel
Sprache:	eng
Schlagworte:	Differential scanning fluorimetry DNA-Binding Proteins - biosynthesis DNA-Binding Proteins - chemistry DNA-Binding Proteins - genetics DNA-Binding Proteins - isolation & purification Escherichia coli - chemistry Escherichia coli - genetics Escherichia coli - metabolism Protein stability Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombination intermediates Saccharomyces cerevisiae - chemistry Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - biosynthesis Saccharomyces cerevisiae Proteins - chemistry Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - isolation & purification Single-strand annealing SYPRO Orange
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