Serotypes, phage types and virulence genes of Shiga-producing Escherichia coli isolated from sheep in Spain
Problem addressed: Shiga toxin-producing Escherichia coli (STEC), have emerged as food poisoning pathogens which can cause severe diseases in humans. Objective: The aim of this study was to determinate the serotypes and virulence genes of STEC strains isolated from sheep in Spain, with the purpose o...
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| Veröffentlicht in: | Veterinary microbiology 2003-06, Vol.94 (1), p.47-56 |
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| creator | Rey, Joaquı́n Blanco, Jesús E Blanco, Miguel Mora, Azucena Dahbi, Ghizlane Alonso, Juan M Hermoso, Miguel Hermoso, Javier Alonso, Marı́a Pilar Usera, Miguel A González, Enrique A Bernárdez, Marı́a I Blanco, Jorge |
| description | Problem addressed: Shiga toxin-producing
Escherichia coli (STEC), have emerged as food poisoning pathogens which can cause severe diseases in humans.
Objective: The aim of this study was to determinate the serotypes and virulence genes of STEC strains isolated from sheep in Spain, with the purpose of determining whether sheep represent a potential source of STEC pathogenic for humans.
Methods and approach: Faecal swabs obtained from 697 healthy lambs on 35 flocks in Spain during the years 2000 and 2001 were examined for STEC using phenotypic (Vero cells) and genotypic (PCR) methods.
Results: STEC O157:H7 strains were isolated from seven (1%) animals in six flocks, whereas non-O157 STEC strains were isolated from 246 (35%) lambs in 33 flocks. A total of 253 ovine STEC strains were identified in this study. PCR showed that 110 (43%) strains carried
stx
1 genes, 10 (4%) possessed
stx
2 genes and 133 (53%) both
stx
1 and
stx
2. Enterohaemolysin (
ehxA) and intimin (
eae) virulence genes were detected in 120 (47%) and in 9 (4%) of the STEC strains. STEC strains belonged to 22 O serogroups and 44 O:H serotypes. However, 70% were of one of these six serogroups (O6, O91, O117, O128, O146, O166) and 71% belonged to only nine serotypes (O6:H10, O76:H19, O91:H–, O117:H–, O128:H–, O128:H2, O146:H21, O157:H7, O166:H28). A total of 10 new O:H serotypes not previously reported in STEC strains were found in this study. Seven strains of serotype O157:H7 possessed intimin type γ1, and two strains of serotype O156:H– had the new intimin ζ. STEC O157:H7 strains were phage types 54 (four strains), 34 (two strains) and 14 (one strain).
Conclusions: This study confirms that healthy sheep are a major reservoir of STEC pathogenic for humans. However, because the
eae gene is present only in a very small proportion of ovine non-O157 STEC, most ovine strains may be less pathogenic. |
| doi_str_mv | 10.1016/S0378-1135(03)00064-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_18748207</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378113503000646</els_id><sourcerecordid>18748207</sourcerecordid><originalsourceid>FETCH-LOGICAL-c446t-292b07c78c28de72a1a6581c56310098b5840edf0bf2a452782400d37e3584933</originalsourceid><addsrcrecordid>eNqF0UuP1SAUB3BiNM6d0Y-gstGMidXDo4WujJmMj2QSF3XWhEtPW7S3dKCdZL693EecpSsC_A4c_hDyisFHBqz61IBQumBMlJcg3gNAJYvqCdkwrUTBS8mfks0_ckbOU_qdkawreE7OGFeSK1ZuyJ8GY1geZkwf6DzYHulhQu3U0nsf1xEnh7THKa-FjjaD720xx9Cuzk89vU5uwOjd4C11YfTUpzDaBVvaxbCjaUCcqZ9oM1s_vSDPOjsmfHkaL8jt1-tfV9-Lm5_fflx9uSmclNVS8JpvQTmlHdctKm6ZrUrNXFkJBlDrbaklYNvBtuNWllxpLgFaoVDknVqIC_LueG7u827FtJidTw7H0U4Y1mRyRFJzUBmWR-hiSCliZ-bodzY-GAZmn7I5pGz2ERoQ5pCyqXLd69MF63aH7WPVKdYM3p6ATc6OXbST8-nRSSXLuq6ze3N0nQ3G9jGb24YDy-8BpSvJs_h8FJgDu_cYTXJ-_yetj-gW0wb_n2b_ApFtoYA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18748207</pqid></control><display><type>article</type><title>Serotypes, phage types and virulence genes of Shiga-producing Escherichia coli isolated from sheep in Spain</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Rey, Joaquı́n ; Blanco, Jesús E ; Blanco, Miguel ; Mora, Azucena ; Dahbi, Ghizlane ; Alonso, Juan M ; Hermoso, Miguel ; Hermoso, Javier ; Alonso, Marı́a Pilar ; Usera, Miguel A ; González, Enrique A ; Bernárdez, Marı́a I ; Blanco, Jorge</creator><creatorcontrib>Rey, Joaquı́n ; Blanco, Jesús E ; Blanco, Miguel ; Mora, Azucena ; Dahbi, Ghizlane ; Alonso, Juan M ; Hermoso, Miguel ; Hermoso, Javier ; Alonso, Marı́a Pilar ; Usera, Miguel A ; González, Enrique A ; Bernárdez, Marı́a I ; Blanco, Jorge</creatorcontrib><description>Problem addressed: Shiga toxin-producing
Escherichia coli (STEC), have emerged as food poisoning pathogens which can cause severe diseases in humans.
Objective: The aim of this study was to determinate the serotypes and virulence genes of STEC strains isolated from sheep in Spain, with the purpose of determining whether sheep represent a potential source of STEC pathogenic for humans.
Methods and approach: Faecal swabs obtained from 697 healthy lambs on 35 flocks in Spain during the years 2000 and 2001 were examined for STEC using phenotypic (Vero cells) and genotypic (PCR) methods.
Results: STEC O157:H7 strains were isolated from seven (1%) animals in six flocks, whereas non-O157 STEC strains were isolated from 246 (35%) lambs in 33 flocks. A total of 253 ovine STEC strains were identified in this study. PCR showed that 110 (43%) strains carried
stx
1 genes, 10 (4%) possessed
stx
2 genes and 133 (53%) both
stx
1 and
stx
2. Enterohaemolysin (
ehxA) and intimin (
eae) virulence genes were detected in 120 (47%) and in 9 (4%) of the STEC strains. STEC strains belonged to 22 O serogroups and 44 O:H serotypes. However, 70% were of one of these six serogroups (O6, O91, O117, O128, O146, O166) and 71% belonged to only nine serotypes (O6:H10, O76:H19, O91:H–, O117:H–, O128:H–, O128:H2, O146:H21, O157:H7, O166:H28). A total of 10 new O:H serotypes not previously reported in STEC strains were found in this study. Seven strains of serotype O157:H7 possessed intimin type γ1, and two strains of serotype O156:H– had the new intimin ζ. STEC O157:H7 strains were phage types 54 (four strains), 34 (two strains) and 14 (one strain).
Conclusions: This study confirms that healthy sheep are a major reservoir of STEC pathogenic for humans. However, because the
eae gene is present only in a very small proportion of ovine non-O157 STEC, most ovine strains may be less pathogenic.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/S0378-1135(03)00064-6</identifier><identifier>PMID: 12742715</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Antigens, Bacterial - metabolism ; Bacteriology ; Bacteriophage Typing - veterinary ; bacteriophages ; Biological and medical sciences ; Cercopithecus aethiops ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; Escherichia coli Infections - epidemiology ; Escherichia coli Infections - microbiology ; Escherichia coli Infections - veterinary ; Escherichia coli O157 - classification ; Escherichia coli O157 - genetics ; Escherichia coli O157 - pathogenicity ; Escherichia coli O157:H7 ; Feces - microbiology ; flocks ; foodborne illness ; Fundamental and applied biological sciences. Psychology ; genes ; HeLa Cells ; Humans ; lambs ; Microbiology ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; poisoning ; polymerase chain reaction ; Polymerase Chain Reaction - veterinary ; Prevalence ; serotypes ; Serotypes of STEC ; Sheep ; Sheep Diseases - microbiology ; Shiga toxin-producing Escherichia coli ; Shiga toxins ; Shiga Toxins - genetics ; Shiga Toxins - metabolism ; Spain - epidemiology ; STEC ; Vero Cells ; Verotoxins ; Virulence ; VTEC</subject><ispartof>Veterinary microbiology, 2003-06, Vol.94 (1), p.47-56</ispartof><rights>2003 Elsevier Science B.V.</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-292b07c78c28de72a1a6581c56310098b5840edf0bf2a452782400d37e3584933</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378113503000646$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14745999$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12742715$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rey, Joaquı́n</creatorcontrib><creatorcontrib>Blanco, Jesús E</creatorcontrib><creatorcontrib>Blanco, Miguel</creatorcontrib><creatorcontrib>Mora, Azucena</creatorcontrib><creatorcontrib>Dahbi, Ghizlane</creatorcontrib><creatorcontrib>Alonso, Juan M</creatorcontrib><creatorcontrib>Hermoso, Miguel</creatorcontrib><creatorcontrib>Hermoso, Javier</creatorcontrib><creatorcontrib>Alonso, Marı́a Pilar</creatorcontrib><creatorcontrib>Usera, Miguel A</creatorcontrib><creatorcontrib>González, Enrique A</creatorcontrib><creatorcontrib>Bernárdez, Marı́a I</creatorcontrib><creatorcontrib>Blanco, Jorge</creatorcontrib><title>Serotypes, phage types and virulence genes of Shiga-producing Escherichia coli isolated from sheep in Spain</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>Problem addressed: Shiga toxin-producing
Escherichia coli (STEC), have emerged as food poisoning pathogens which can cause severe diseases in humans.
Objective: The aim of this study was to determinate the serotypes and virulence genes of STEC strains isolated from sheep in Spain, with the purpose of determining whether sheep represent a potential source of STEC pathogenic for humans.
Methods and approach: Faecal swabs obtained from 697 healthy lambs on 35 flocks in Spain during the years 2000 and 2001 were examined for STEC using phenotypic (Vero cells) and genotypic (PCR) methods.
Results: STEC O157:H7 strains were isolated from seven (1%) animals in six flocks, whereas non-O157 STEC strains were isolated from 246 (35%) lambs in 33 flocks. A total of 253 ovine STEC strains were identified in this study. PCR showed that 110 (43%) strains carried
stx
1 genes, 10 (4%) possessed
stx
2 genes and 133 (53%) both
stx
1 and
stx
2. Enterohaemolysin (
ehxA) and intimin (
eae) virulence genes were detected in 120 (47%) and in 9 (4%) of the STEC strains. STEC strains belonged to 22 O serogroups and 44 O:H serotypes. However, 70% were of one of these six serogroups (O6, O91, O117, O128, O146, O166) and 71% belonged to only nine serotypes (O6:H10, O76:H19, O91:H–, O117:H–, O128:H–, O128:H2, O146:H21, O157:H7, O166:H28). A total of 10 new O:H serotypes not previously reported in STEC strains were found in this study. Seven strains of serotype O157:H7 possessed intimin type γ1, and two strains of serotype O156:H– had the new intimin ζ. STEC O157:H7 strains were phage types 54 (four strains), 34 (two strains) and 14 (one strain).
Conclusions: This study confirms that healthy sheep are a major reservoir of STEC pathogenic for humans. However, because the
eae gene is present only in a very small proportion of ovine non-O157 STEC, most ovine strains may be less pathogenic.</description><subject>Animals</subject><subject>Antigens, Bacterial - metabolism</subject><subject>Bacteriology</subject><subject>Bacteriophage Typing - veterinary</subject><subject>bacteriophages</subject><subject>Biological and medical sciences</subject><subject>Cercopithecus aethiops</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>Escherichia coli Infections - epidemiology</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli Infections - veterinary</subject><subject>Escherichia coli O157 - classification</subject><subject>Escherichia coli O157 - genetics</subject><subject>Escherichia coli O157 - pathogenicity</subject><subject>Escherichia coli O157:H7</subject><subject>Feces - microbiology</subject><subject>flocks</subject><subject>foodborne illness</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>lambs</subject><subject>Microbiology</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>poisoning</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Prevalence</subject><subject>serotypes</subject><subject>Serotypes of STEC</subject><subject>Sheep</subject><subject>Sheep Diseases - microbiology</subject><subject>Shiga toxin-producing Escherichia coli</subject><subject>Shiga toxins</subject><subject>Shiga Toxins - genetics</subject><subject>Shiga Toxins - metabolism</subject><subject>Spain - epidemiology</subject><subject>STEC</subject><subject>Vero Cells</subject><subject>Verotoxins</subject><subject>Virulence</subject><subject>VTEC</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0UuP1SAUB3BiNM6d0Y-gstGMidXDo4WujJmMj2QSF3XWhEtPW7S3dKCdZL693EecpSsC_A4c_hDyisFHBqz61IBQumBMlJcg3gNAJYvqCdkwrUTBS8mfks0_ckbOU_qdkawreE7OGFeSK1ZuyJ8GY1geZkwf6DzYHulhQu3U0nsf1xEnh7THKa-FjjaD720xx9Cuzk89vU5uwOjd4C11YfTUpzDaBVvaxbCjaUCcqZ9oM1s_vSDPOjsmfHkaL8jt1-tfV9-Lm5_fflx9uSmclNVS8JpvQTmlHdctKm6ZrUrNXFkJBlDrbaklYNvBtuNWllxpLgFaoVDknVqIC_LueG7u827FtJidTw7H0U4Y1mRyRFJzUBmWR-hiSCliZ-bodzY-GAZmn7I5pGz2ERoQ5pCyqXLd69MF63aH7WPVKdYM3p6ATc6OXbST8-nRSSXLuq6ze3N0nQ3G9jGb24YDy-8BpSvJs_h8FJgDu_cYTXJ-_yetj-gW0wb_n2b_ApFtoYA</recordid><startdate>20030624</startdate><enddate>20030624</enddate><creator>Rey, Joaquı́n</creator><creator>Blanco, Jesús E</creator><creator>Blanco, Miguel</creator><creator>Mora, Azucena</creator><creator>Dahbi, Ghizlane</creator><creator>Alonso, Juan M</creator><creator>Hermoso, Miguel</creator><creator>Hermoso, Javier</creator><creator>Alonso, Marı́a Pilar</creator><creator>Usera, Miguel A</creator><creator>González, Enrique A</creator><creator>Bernárdez, Marı́a I</creator><creator>Blanco, Jorge</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20030624</creationdate><title>Serotypes, phage types and virulence genes of Shiga-producing Escherichia coli isolated from sheep in Spain</title><author>Rey, Joaquı́n ; Blanco, Jesús E ; Blanco, Miguel ; Mora, Azucena ; Dahbi, Ghizlane ; Alonso, Juan M ; Hermoso, Miguel ; Hermoso, Javier ; Alonso, Marı́a Pilar ; Usera, Miguel A ; González, Enrique A ; Bernárdez, Marı́a I ; Blanco, Jorge</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-292b07c78c28de72a1a6581c56310098b5840edf0bf2a452782400d37e3584933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Antigens, Bacterial - metabolism</topic><topic>Bacteriology</topic><topic>Bacteriophage Typing - veterinary</topic><topic>bacteriophages</topic><topic>Biological and medical sciences</topic><topic>Cercopithecus aethiops</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>Escherichia coli Infections - epidemiology</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Escherichia coli Infections - veterinary</topic><topic>Escherichia coli O157 - classification</topic><topic>Escherichia coli O157 - genetics</topic><topic>Escherichia coli O157 - pathogenicity</topic><topic>Escherichia coli O157:H7</topic><topic>Feces - microbiology</topic><topic>flocks</topic><topic>foodborne illness</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>lambs</topic><topic>Microbiology</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>poisoning</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Prevalence</topic><topic>serotypes</topic><topic>Serotypes of STEC</topic><topic>Sheep</topic><topic>Sheep Diseases - microbiology</topic><topic>Shiga toxin-producing Escherichia coli</topic><topic>Shiga toxins</topic><topic>Shiga Toxins - genetics</topic><topic>Shiga Toxins - metabolism</topic><topic>Spain - epidemiology</topic><topic>STEC</topic><topic>Vero Cells</topic><topic>Verotoxins</topic><topic>Virulence</topic><topic>VTEC</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rey, Joaquı́n</creatorcontrib><creatorcontrib>Blanco, Jesús E</creatorcontrib><creatorcontrib>Blanco, Miguel</creatorcontrib><creatorcontrib>Mora, Azucena</creatorcontrib><creatorcontrib>Dahbi, Ghizlane</creatorcontrib><creatorcontrib>Alonso, Juan M</creatorcontrib><creatorcontrib>Hermoso, Miguel</creatorcontrib><creatorcontrib>Hermoso, Javier</creatorcontrib><creatorcontrib>Alonso, Marı́a Pilar</creatorcontrib><creatorcontrib>Usera, Miguel A</creatorcontrib><creatorcontrib>González, Enrique A</creatorcontrib><creatorcontrib>Bernárdez, Marı́a I</creatorcontrib><creatorcontrib>Blanco, Jorge</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rey, Joaquı́n</au><au>Blanco, Jesús E</au><au>Blanco, Miguel</au><au>Mora, Azucena</au><au>Dahbi, Ghizlane</au><au>Alonso, Juan M</au><au>Hermoso, Miguel</au><au>Hermoso, Javier</au><au>Alonso, Marı́a Pilar</au><au>Usera, Miguel A</au><au>González, Enrique A</au><au>Bernárdez, Marı́a I</au><au>Blanco, Jorge</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serotypes, phage types and virulence genes of Shiga-producing Escherichia coli isolated from sheep in Spain</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2003-06-24</date><risdate>2003</risdate><volume>94</volume><issue>1</issue><spage>47</spage><epage>56</epage><pages>47-56</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>Problem addressed: Shiga toxin-producing
Escherichia coli (STEC), have emerged as food poisoning pathogens which can cause severe diseases in humans.
Objective: The aim of this study was to determinate the serotypes and virulence genes of STEC strains isolated from sheep in Spain, with the purpose of determining whether sheep represent a potential source of STEC pathogenic for humans.
Methods and approach: Faecal swabs obtained from 697 healthy lambs on 35 flocks in Spain during the years 2000 and 2001 were examined for STEC using phenotypic (Vero cells) and genotypic (PCR) methods.
Results: STEC O157:H7 strains were isolated from seven (1%) animals in six flocks, whereas non-O157 STEC strains were isolated from 246 (35%) lambs in 33 flocks. A total of 253 ovine STEC strains were identified in this study. PCR showed that 110 (43%) strains carried
stx
1 genes, 10 (4%) possessed
stx
2 genes and 133 (53%) both
stx
1 and
stx
2. Enterohaemolysin (
ehxA) and intimin (
eae) virulence genes were detected in 120 (47%) and in 9 (4%) of the STEC strains. STEC strains belonged to 22 O serogroups and 44 O:H serotypes. However, 70% were of one of these six serogroups (O6, O91, O117, O128, O146, O166) and 71% belonged to only nine serotypes (O6:H10, O76:H19, O91:H–, O117:H–, O128:H–, O128:H2, O146:H21, O157:H7, O166:H28). A total of 10 new O:H serotypes not previously reported in STEC strains were found in this study. Seven strains of serotype O157:H7 possessed intimin type γ1, and two strains of serotype O156:H– had the new intimin ζ. STEC O157:H7 strains were phage types 54 (four strains), 34 (two strains) and 14 (one strain).
Conclusions: This study confirms that healthy sheep are a major reservoir of STEC pathogenic for humans. However, because the
eae gene is present only in a very small proportion of ovine non-O157 STEC, most ovine strains may be less pathogenic.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>12742715</pmid><doi>10.1016/S0378-1135(03)00064-6</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Veterinary microbiology, 2003-06, Vol.94 (1), p.47-56 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Antigens, Bacterial - metabolism Bacteriology Bacteriophage Typing - veterinary bacteriophages Biological and medical sciences Cercopithecus aethiops DNA, Bacterial - chemistry DNA, Bacterial - genetics Escherichia coli Infections - epidemiology Escherichia coli Infections - microbiology Escherichia coli Infections - veterinary Escherichia coli O157 - classification Escherichia coli O157 - genetics Escherichia coli O157 - pathogenicity Escherichia coli O157:H7 Feces - microbiology flocks foodborne illness Fundamental and applied biological sciences. Psychology genes HeLa Cells Humans lambs Microbiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains poisoning polymerase chain reaction Polymerase Chain Reaction - veterinary Prevalence serotypes Serotypes of STEC Sheep Sheep Diseases - microbiology Shiga toxin-producing Escherichia coli Shiga toxins Shiga Toxins - genetics Shiga Toxins - metabolism Spain - epidemiology STEC Vero Cells Verotoxins Virulence VTEC |
| title | Serotypes, phage types and virulence genes of Shiga-producing Escherichia coli isolated from sheep in Spain |
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