Simultaneous detection and identification of STI pathogens by multiplex Real-Time PCR in genital tract specimens in a selected area of Apulia, a region of Southern Italy
Purpose Genital tract infections are globally a major cause of morbidity in sexually active individuals. The aim of this study was to investigate the prevalence and associations of co-infections of Chlamydia trachomatis , Neisseria gonorrhoeae , Trichomonas vaginalis , Mycoplasma hominis (MH), Mycop...
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creator | Del Prete, Raffaele Ronga, Luigi Lestingi, Mirella Addati, Grazia Angelotti, Umberto Filippo Di Carlo, Domenico Miragliotta, Giuseppe |
description | Purpose
Genital tract infections are globally a major cause of morbidity in sexually active individuals. The aim of this study was to investigate the prevalence and associations of co-infections of
Chlamydia trachomatis
,
Neisseria gonorrhoeae
,
Trichomonas vaginalis
,
Mycoplasma hominis
(MH),
Mycoplasma genitalium
,
Ureaplasma urealyticum
(UU) and
Ureaplasma parvum
(UP) in specimens collected from female (SF) and male (SM) patients.
Methods
1575 samples from 1575 individuals from the geographical area around Bari, Apulia region in Southern Italy, were collected and analyzed by a multiplex Real-Time PCR (mRT-PCR) (Anyplex
TM
II STI-7, Seegene, Inc., Seoul, Korea) assay.
Results
455/1575 (28.89%) samples resulted positive for at least one of the targets named above. Statistically significant differences in prevalence of the pathogens between SF and SM were not detected except for UP (24.92% in SF vs 8.91% in SM). Prevalence of co-infections was 6.84 and 3.96% in SF and SM, respectively. Moreover, MH presence in SF, but not in SM, was associated with UU and UP.
Conclusions
Our data suggest different patterns of infections between females and male and the importance of an increased vigilance of sexually transmitted pathogens to reduce the burden on general population and the sequelae or the complications on reproductive organs. |
doi_str_mv | 10.1007/s15010-017-1002-7 |
format | Article |
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Genital tract infections are globally a major cause of morbidity in sexually active individuals. The aim of this study was to investigate the prevalence and associations of co-infections of
Chlamydia trachomatis
,
Neisseria gonorrhoeae
,
Trichomonas vaginalis
,
Mycoplasma hominis
(MH),
Mycoplasma genitalium
,
Ureaplasma urealyticum
(UU) and
Ureaplasma parvum
(UP) in specimens collected from female (SF) and male (SM) patients.
Methods
1575 samples from 1575 individuals from the geographical area around Bari, Apulia region in Southern Italy, were collected and analyzed by a multiplex Real-Time PCR (mRT-PCR) (Anyplex
TM
II STI-7, Seegene, Inc., Seoul, Korea) assay.
Results
455/1575 (28.89%) samples resulted positive for at least one of the targets named above. Statistically significant differences in prevalence of the pathogens between SF and SM were not detected except for UP (24.92% in SF vs 8.91% in SM). Prevalence of co-infections was 6.84 and 3.96% in SF and SM, respectively. Moreover, MH presence in SF, but not in SM, was associated with UU and UP.
Conclusions
Our data suggest different patterns of infections between females and male and the importance of an increased vigilance of sexually transmitted pathogens to reduce the burden on general population and the sequelae or the complications on reproductive organs.</description><identifier>ISSN: 0300-8126</identifier><identifier>EISSN: 1439-0973</identifier><identifier>DOI: 10.1007/s15010-017-1002-7</identifier><identifier>PMID: 28260146</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adult ; Bacteria ; Bacteria - isolation & purification ; Coinfection - epidemiology ; Coinfection - microbiology ; Complications ; Family Medicine ; Female ; Females ; General Practice ; Genital tract ; Humans ; Infections ; Infectious Diseases ; Internal Medicine ; Italy - epidemiology ; Male ; Medicine ; Medicine & Public Health ; Middle Aged ; Morbidity ; Multiplex Polymerase Chain Reaction ; Multiplexing ; Organs ; Original Paper ; Pathogens ; Polymerase chain reaction ; Prevalence ; Real time ; Real-Time Polymerase Chain Reaction ; Reproductive organs ; Sexually transmitted diseases ; Sexually Transmitted Diseases - epidemiology ; Sexually Transmitted Diseases - microbiology ; Statistical analysis ; Statistical methods ; STD ; Vigilance</subject><ispartof>Infection, 2017-08, Vol.45 (4), p.469-477</ispartof><rights>Springer-Verlag Berlin Heidelberg 2017</rights><rights>Infection is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-e7f1d3d5f11c1621fe5d4acb985e4f6fcac90f1cb240b6d3ec1781e44ad9c5263</citedby><cites>FETCH-LOGICAL-c372t-e7f1d3d5f11c1621fe5d4acb985e4f6fcac90f1cb240b6d3ec1781e44ad9c5263</cites><orcidid>0000-0002-9409-3388</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s15010-017-1002-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s15010-017-1002-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28260146$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Del Prete, Raffaele</creatorcontrib><creatorcontrib>Ronga, Luigi</creatorcontrib><creatorcontrib>Lestingi, Mirella</creatorcontrib><creatorcontrib>Addati, Grazia</creatorcontrib><creatorcontrib>Angelotti, Umberto Filippo</creatorcontrib><creatorcontrib>Di Carlo, Domenico</creatorcontrib><creatorcontrib>Miragliotta, Giuseppe</creatorcontrib><title>Simultaneous detection and identification of STI pathogens by multiplex Real-Time PCR in genital tract specimens in a selected area of Apulia, a region of Southern Italy</title><title>Infection</title><addtitle>Infection</addtitle><addtitle>Infection</addtitle><description>Purpose
Genital tract infections are globally a major cause of morbidity in sexually active individuals. The aim of this study was to investigate the prevalence and associations of co-infections of
Chlamydia trachomatis
,
Neisseria gonorrhoeae
,
Trichomonas vaginalis
,
Mycoplasma hominis
(MH),
Mycoplasma genitalium
,
Ureaplasma urealyticum
(UU) and
Ureaplasma parvum
(UP) in specimens collected from female (SF) and male (SM) patients.
Methods
1575 samples from 1575 individuals from the geographical area around Bari, Apulia region in Southern Italy, were collected and analyzed by a multiplex Real-Time PCR (mRT-PCR) (Anyplex
TM
II STI-7, Seegene, Inc., Seoul, Korea) assay.
Results
455/1575 (28.89%) samples resulted positive for at least one of the targets named above. Statistically significant differences in prevalence of the pathogens between SF and SM were not detected except for UP (24.92% in SF vs 8.91% in SM). Prevalence of co-infections was 6.84 and 3.96% in SF and SM, respectively. Moreover, MH presence in SF, but not in SM, was associated with UU and UP.
Conclusions
Our data suggest different patterns of infections between females and male and the importance of an increased vigilance of sexually transmitted pathogens to reduce the burden on general population and the sequelae or the complications on reproductive organs.</description><subject>Adult</subject><subject>Bacteria</subject><subject>Bacteria - isolation & purification</subject><subject>Coinfection - epidemiology</subject><subject>Coinfection - microbiology</subject><subject>Complications</subject><subject>Family Medicine</subject><subject>Female</subject><subject>Females</subject><subject>General Practice</subject><subject>Genital tract</subject><subject>Humans</subject><subject>Infections</subject><subject>Infectious Diseases</subject><subject>Internal Medicine</subject><subject>Italy - epidemiology</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Morbidity</subject><subject>Multiplex Polymerase Chain Reaction</subject><subject>Multiplexing</subject><subject>Organs</subject><subject>Original Paper</subject><subject>Pathogens</subject><subject>Polymerase chain reaction</subject><subject>Prevalence</subject><subject>Real time</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Reproductive organs</subject><subject>Sexually transmitted diseases</subject><subject>Sexually Transmitted Diseases - epidemiology</subject><subject>Sexually Transmitted Diseases - microbiology</subject><subject>Statistical analysis</subject><subject>Statistical methods</subject><subject>STD</subject><subject>Vigilance</subject><issn>0300-8126</issn><issn>1439-0973</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp1kV9rFDEUxYModq1-AF8k4IsPHc1NMpOZx7JoXSgo7fo8ZJKbbcr8M8mA-5H8lmadVkTwKdyc3zk35BDyGth7YEx9iFAyYAUDVeSZF-oJ2YAUTcEaJZ6SDROMFTXw6oy8iPGeMVY2Uj0nZ7zmFQNZbcjPWz8sfdIjTkukFhOa5KeR6tFSb3FM3nmjf19Njt7ud3TW6W464Bhpd6Qnr597_EFvUPfF3g9Iv25vqB9pRnzSPU1Bm0TjjCaL2ZUlTSP2eRFaqgPqU_LlvPReX2Qp4OFx27SkOwwj3eWc40vyzOk-4quH85x8-_Rxv_1cXH-52m0vrwsjFE8FKgdW2NIBGKg4OCyt1KZr6hKlq5zRpmEOTMcl6yor0ICqAaXUtjElr8Q5ebfmzmH6vmBM7eCjwb5f_6iFWklVl0rIjL79B72fljDm17XQcFGBqJnIFKyUCVOMAV07Bz_ocGyBtace27XHNvd4mnmrsufNQ_LSDWj_OB6LywBfgZil8YDhr9X_Tf0Fh9yqHg</recordid><startdate>20170801</startdate><enddate>20170801</enddate><creator>Del Prete, Raffaele</creator><creator>Ronga, Luigi</creator><creator>Lestingi, Mirella</creator><creator>Addati, Grazia</creator><creator>Angelotti, Umberto Filippo</creator><creator>Di Carlo, Domenico</creator><creator>Miragliotta, Giuseppe</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9409-3388</orcidid></search><sort><creationdate>20170801</creationdate><title>Simultaneous detection and identification of STI pathogens by multiplex Real-Time PCR in genital tract specimens in a selected area of Apulia, a region of Southern Italy</title><author>Del Prete, Raffaele ; Ronga, Luigi ; Lestingi, Mirella ; Addati, Grazia ; Angelotti, Umberto Filippo ; Di Carlo, Domenico ; Miragliotta, Giuseppe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-e7f1d3d5f11c1621fe5d4acb985e4f6fcac90f1cb240b6d3ec1781e44ad9c5263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Bacteria</topic><topic>Bacteria - isolation & purification</topic><topic>Coinfection - epidemiology</topic><topic>Coinfection - microbiology</topic><topic>Complications</topic><topic>Family Medicine</topic><topic>Female</topic><topic>Females</topic><topic>General Practice</topic><topic>Genital tract</topic><topic>Humans</topic><topic>Infections</topic><topic>Infectious Diseases</topic><topic>Internal Medicine</topic><topic>Italy - epidemiology</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>Morbidity</topic><topic>Multiplex Polymerase Chain Reaction</topic><topic>Multiplexing</topic><topic>Organs</topic><topic>Original Paper</topic><topic>Pathogens</topic><topic>Polymerase chain reaction</topic><topic>Prevalence</topic><topic>Real time</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Reproductive organs</topic><topic>Sexually transmitted diseases</topic><topic>Sexually Transmitted Diseases - epidemiology</topic><topic>Sexually Transmitted Diseases - microbiology</topic><topic>Statistical analysis</topic><topic>Statistical methods</topic><topic>STD</topic><topic>Vigilance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Del Prete, Raffaele</creatorcontrib><creatorcontrib>Ronga, Luigi</creatorcontrib><creatorcontrib>Lestingi, Mirella</creatorcontrib><creatorcontrib>Addati, Grazia</creatorcontrib><creatorcontrib>Angelotti, Umberto Filippo</creatorcontrib><creatorcontrib>Di Carlo, Domenico</creatorcontrib><creatorcontrib>Miragliotta, Giuseppe</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nursing & Allied Health Database</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Del Prete, Raffaele</au><au>Ronga, Luigi</au><au>Lestingi, Mirella</au><au>Addati, Grazia</au><au>Angelotti, Umberto Filippo</au><au>Di Carlo, Domenico</au><au>Miragliotta, Giuseppe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous detection and identification of STI pathogens by multiplex Real-Time PCR in genital tract specimens in a selected area of Apulia, a region of Southern Italy</atitle><jtitle>Infection</jtitle><stitle>Infection</stitle><addtitle>Infection</addtitle><date>2017-08-01</date><risdate>2017</risdate><volume>45</volume><issue>4</issue><spage>469</spage><epage>477</epage><pages>469-477</pages><issn>0300-8126</issn><eissn>1439-0973</eissn><abstract>Purpose
Genital tract infections are globally a major cause of morbidity in sexually active individuals. The aim of this study was to investigate the prevalence and associations of co-infections of
Chlamydia trachomatis
,
Neisseria gonorrhoeae
,
Trichomonas vaginalis
,
Mycoplasma hominis
(MH),
Mycoplasma genitalium
,
Ureaplasma urealyticum
(UU) and
Ureaplasma parvum
(UP) in specimens collected from female (SF) and male (SM) patients.
Methods
1575 samples from 1575 individuals from the geographical area around Bari, Apulia region in Southern Italy, were collected and analyzed by a multiplex Real-Time PCR (mRT-PCR) (Anyplex
TM
II STI-7, Seegene, Inc., Seoul, Korea) assay.
Results
455/1575 (28.89%) samples resulted positive for at least one of the targets named above. Statistically significant differences in prevalence of the pathogens between SF and SM were not detected except for UP (24.92% in SF vs 8.91% in SM). Prevalence of co-infections was 6.84 and 3.96% in SF and SM, respectively. Moreover, MH presence in SF, but not in SM, was associated with UU and UP.
Conclusions
Our data suggest different patterns of infections between females and male and the importance of an increased vigilance of sexually transmitted pathogens to reduce the burden on general population and the sequelae or the complications on reproductive organs.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>28260146</pmid><doi>10.1007/s15010-017-1002-7</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-9409-3388</orcidid></addata></record> |
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language | eng |
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source | MEDLINE; SpringerLink Journals - AutoHoldings |
subjects | Adult Bacteria Bacteria - isolation & purification Coinfection - epidemiology Coinfection - microbiology Complications Family Medicine Female Females General Practice Genital tract Humans Infections Infectious Diseases Internal Medicine Italy - epidemiology Male Medicine Medicine & Public Health Middle Aged Morbidity Multiplex Polymerase Chain Reaction Multiplexing Organs Original Paper Pathogens Polymerase chain reaction Prevalence Real time Real-Time Polymerase Chain Reaction Reproductive organs Sexually transmitted diseases Sexually Transmitted Diseases - epidemiology Sexually Transmitted Diseases - microbiology Statistical analysis Statistical methods STD Vigilance |
title | Simultaneous detection and identification of STI pathogens by multiplex Real-Time PCR in genital tract specimens in a selected area of Apulia, a region of Southern Italy |
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