Sperm RNA preparation for transcriptomic analysis: Review of the techniques and personal experience

Summary In the last 10 years, several approaches, including microarrays, have been applied to investigate sperm transcript levels. However, success using microarray profiling is highly dependent of the quality of the RNA obtained. Therefore, the development of methods that deliver highly purified an...

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Veröffentlicht in:	Andrologia 2017-12, Vol.49 (10), p.e12767-n/a
Hauptverfasser:	El Fekih, S., Nguyen, M.‐H., Perrin, A., Beauvillard, D., Morel, F., Saad, A., Ben Ali, H., De Braekeleer, M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Capacitation Centrifugation DNA microarrays Embryogenesis Fertilization Gene Expression Profiling - methods Gene regulation Humans Infertility, Male - genetics Infertility, Male - metabolism Lysis Male microarray Purification Reviews RNA - analysis RNA isolation RNA quality Sperm sperm purification sperm RNA Spermatogenesis Spermatozoa - metabolism Transcription
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