Concentration of Zinc, Copper, Iron, Calcium, and Magnesium in the Serum, Tissues, and Urine of Streptozotocin-Induced Mild Diabetic Rat Model
The present study aimed to investigate, in the streptozotocin-induced mild diabetic rat model, the zinc (Zn), copper (Cu), iron (Fe), calcium (Ca), and magnesium (Mg) concentration in serum, liver, and kidney tissues, and urine samples from adult Wistar rats treated neonatally with streptozotocin (S...
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| Veröffentlicht in: | Biological trace element research 2017-10, Vol.179 (2), p.237-246 |
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| creator | Gómez, Tahiry Bequer, Leticia Mollineda, Angel Molina, José L. Álvarez, Alain Lavastida, Mayrelis Clapés, Sonia |
| description | The present study aimed to investigate, in the streptozotocin-induced mild diabetic rat model, the zinc (Zn), copper (Cu), iron (Fe), calcium (Ca), and magnesium (Mg) concentration in serum, liver, and kidney tissues, and urine samples from adult Wistar rats treated neonatally with streptozotocin (STZ). Diabetes was induced by subcutaneous administration of streptozotocin (100 mg/Kg) in female Wistar rats of 2 days old (STZ,
n
= 10). Control group (CG,
n
= 10) received only sodium-citrate buffer. The mineral concentrations were measured by atomic absorption spectrophotometry. The validity and accuracy were checked by conventional methods. STZ neonatal injection successfully leaded to mild diabetes in the adult rats. Serum concentrations of Zn, Cu, Fe, Ca, and Mg showed no changes (
p
> 0.05) due to diabetes. The Zn, Fe, Ca, and Mg concentrations in liver and kidney tissues were not different (
p
> 0.05) between STZ and CG. The mean values of Cu were higher (
p
|
| doi_str_mv | 10.1007/s12011-017-0962-x |
| format | Article |
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n
= 10). Control group (CG,
n
= 10) received only sodium-citrate buffer. The mineral concentrations were measured by atomic absorption spectrophotometry. The validity and accuracy were checked by conventional methods. STZ neonatal injection successfully leaded to mild diabetes in the adult rats. Serum concentrations of Zn, Cu, Fe, Ca, and Mg showed no changes (
p
> 0.05) due to diabetes. The Zn, Fe, Ca, and Mg concentrations in liver and kidney tissues were not different (
p
> 0.05) between STZ and CG. The mean values of Cu were higher (
p
< 0.05) in liver and kidney samples from STZ as compared to CG. Urine minerals concentrations (Zn, Cu, Fe and Ca) in STZ-rats group were lower (
p
< 0.05) than CG. However, the content of all evaluated minerals in the excreted urine were higher (
p
< 0.01) in STZ-rats during a 24 h collection period. Urinary excretion of Zn, Cu, Fe, Ca, and Mg was strongly correlated with urinary volume during the 24 h period (
r
> 0.7;
p
< 0.001). Observed changes in mineral metabolism of STZ-induced mild diabetes model could be due to the endocrine imbalance associated with the diabetic condition.</description><identifier>ISSN: 0163-4984</identifier><identifier>EISSN: 1559-0720</identifier><identifier>DOI: 10.1007/s12011-017-0962-x</identifier><identifier>PMID: 28258359</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animal models ; Atomic absorption analysis ; Atomic absorption spectrophotometry ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Calcium ; Citric acid ; Copper ; Diabetes ; Diabetes mellitus ; Dietary minerals ; Iron ; Kidneys ; Life Sciences ; Liver ; Magnesium ; Mineral metabolism ; Minerals ; Neonates ; Nutrition ; Oncology ; Sodium citrate ; Spectral analysis ; Spectrophotometry ; Streptozocin ; Urine ; Zinc</subject><ispartof>Biological trace element research, 2017-10, Vol.179 (2), p.237-246</ispartof><rights>Springer Science+Business Media New York 2017</rights><rights>Biological Trace Element Research is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-5d114dccda8c9bf0d7d69ed9e018c47a32a498bd1099783742b2e2bc064764163</citedby><cites>FETCH-LOGICAL-c438t-5d114dccda8c9bf0d7d69ed9e018c47a32a498bd1099783742b2e2bc064764163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12011-017-0962-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12011-017-0962-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28258359$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gómez, Tahiry</creatorcontrib><creatorcontrib>Bequer, Leticia</creatorcontrib><creatorcontrib>Mollineda, Angel</creatorcontrib><creatorcontrib>Molina, José L.</creatorcontrib><creatorcontrib>Álvarez, Alain</creatorcontrib><creatorcontrib>Lavastida, Mayrelis</creatorcontrib><creatorcontrib>Clapés, Sonia</creatorcontrib><title>Concentration of Zinc, Copper, Iron, Calcium, and Magnesium in the Serum, Tissues, and Urine of Streptozotocin-Induced Mild Diabetic Rat Model</title><title>Biological trace element research</title><addtitle>Biol Trace Elem Res</addtitle><addtitle>Biol Trace Elem Res</addtitle><description>The present study aimed to investigate, in the streptozotocin-induced mild diabetic rat model, the zinc (Zn), copper (Cu), iron (Fe), calcium (Ca), and magnesium (Mg) concentration in serum, liver, and kidney tissues, and urine samples from adult Wistar rats treated neonatally with streptozotocin (STZ). Diabetes was induced by subcutaneous administration of streptozotocin (100 mg/Kg) in female Wistar rats of 2 days old (STZ,
n
= 10). Control group (CG,
n
= 10) received only sodium-citrate buffer. The mineral concentrations were measured by atomic absorption spectrophotometry. The validity and accuracy were checked by conventional methods. STZ neonatal injection successfully leaded to mild diabetes in the adult rats. Serum concentrations of Zn, Cu, Fe, Ca, and Mg showed no changes (
p
> 0.05) due to diabetes. The Zn, Fe, Ca, and Mg concentrations in liver and kidney tissues were not different (
p
> 0.05) between STZ and CG. The mean values of Cu were higher (
p
< 0.05) in liver and kidney samples from STZ as compared to CG. Urine minerals concentrations (Zn, Cu, Fe and Ca) in STZ-rats group were lower (
p
< 0.05) than CG. However, the content of all evaluated minerals in the excreted urine were higher (
p
< 0.01) in STZ-rats during a 24 h collection period. Urinary excretion of Zn, Cu, Fe, Ca, and Mg was strongly correlated with urinary volume during the 24 h period (
r
> 0.7;
p
< 0.001). Observed changes in mineral metabolism of STZ-induced mild diabetes model could be due to the endocrine imbalance associated with the diabetic condition.</description><subject>Animal models</subject><subject>Atomic absorption analysis</subject><subject>Atomic absorption spectrophotometry</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Calcium</subject><subject>Citric acid</subject><subject>Copper</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Dietary minerals</subject><subject>Iron</subject><subject>Kidneys</subject><subject>Life Sciences</subject><subject>Liver</subject><subject>Magnesium</subject><subject>Mineral metabolism</subject><subject>Minerals</subject><subject>Neonates</subject><subject>Nutrition</subject><subject>Oncology</subject><subject>Sodium citrate</subject><subject>Spectral analysis</subject><subject>Spectrophotometry</subject><subject>Streptozocin</subject><subject>Urine</subject><subject>Zinc</subject><issn>0163-4984</issn><issn>1559-0720</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kc9uFSEUh4nR2Gv1AdwYEjcuLgoMMwNLc-ufm7Qxse3GDWHg3EozF0ZgkupD-MwymWqMiStycr7z48CH0HNGXzNK-zeZccoYoawnVHWc3D1AG9a2itCe04doQ1nXEKGkOEFPcr6lFeSqeYxOuOStbFq1QT93MVgIJZniY8DxgL_4YLd4F6cJ0hbvUwy1MqP183GLTXD4wtwEyLXEPuDyFfAlpKV35XOeIa_QdfIBlrjLkmAq8Ucs0fpA9sHNFmqIHx0-82aA4i3-bAq-iA7Gp-jRwYwZnt2fp-j6_bur3Udy_unDfvf2nFjRyEJax5hw1jojrRoO1PWuU-AUUCat6E3DTX324BhVqpdNL_jAgQ-WdqLvRP2VU_RqzZ1S_FaXLvros4VxNAHinDWTvRCi5VJV9OU_6G2cU6jbaaaaTgjWNAvFVsqmmHOCg56SP5r0XTOqF1l6laWrA73I0nd15sV98jwcwf2Z-G2nAnwFcm2FG0h_Xf3f1F8diZ7w</recordid><startdate>20171001</startdate><enddate>20171001</enddate><creator>Gómez, Tahiry</creator><creator>Bequer, Leticia</creator><creator>Mollineda, Angel</creator><creator>Molina, José L.</creator><creator>Álvarez, Alain</creator><creator>Lavastida, Mayrelis</creator><creator>Clapés, Sonia</creator><general>Springer US</general><general>Springer Nature 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of Zinc, Copper, Iron, Calcium, and Magnesium in the Serum, Tissues, and Urine of Streptozotocin-Induced Mild Diabetic Rat Model</title><author>Gómez, Tahiry ; Bequer, Leticia ; Mollineda, Angel ; Molina, José L. ; Álvarez, Alain ; Lavastida, Mayrelis ; Clapés, Sonia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-5d114dccda8c9bf0d7d69ed9e018c47a32a498bd1099783742b2e2bc064764163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animal models</topic><topic>Atomic absorption analysis</topic><topic>Atomic absorption spectrophotometry</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Calcium</topic><topic>Citric acid</topic><topic>Copper</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Dietary minerals</topic><topic>Iron</topic><topic>Kidneys</topic><topic>Life Sciences</topic><topic>Liver</topic><topic>Magnesium</topic><topic>Mineral metabolism</topic><topic>Minerals</topic><topic>Neonates</topic><topic>Nutrition</topic><topic>Oncology</topic><topic>Sodium citrate</topic><topic>Spectral analysis</topic><topic>Spectrophotometry</topic><topic>Streptozocin</topic><topic>Urine</topic><topic>Zinc</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gómez, Tahiry</creatorcontrib><creatorcontrib>Bequer, Leticia</creatorcontrib><creatorcontrib>Mollineda, Angel</creatorcontrib><creatorcontrib>Molina, José L.</creatorcontrib><creatorcontrib>Álvarez, Alain</creatorcontrib><creatorcontrib>Lavastida, Mayrelis</creatorcontrib><creatorcontrib>Clapés, Sonia</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aqualine</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Oceanic 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Copper, Iron, Calcium, and Magnesium in the Serum, Tissues, and Urine of Streptozotocin-Induced Mild Diabetic Rat Model</atitle><jtitle>Biological trace element research</jtitle><stitle>Biol Trace Elem Res</stitle><addtitle>Biol Trace Elem Res</addtitle><date>2017-10-01</date><risdate>2017</risdate><volume>179</volume><issue>2</issue><spage>237</spage><epage>246</epage><pages>237-246</pages><issn>0163-4984</issn><eissn>1559-0720</eissn><abstract>The present study aimed to investigate, in the streptozotocin-induced mild diabetic rat model, the zinc (Zn), copper (Cu), iron (Fe), calcium (Ca), and magnesium (Mg) concentration in serum, liver, and kidney tissues, and urine samples from adult Wistar rats treated neonatally with streptozotocin (STZ). Diabetes was induced by subcutaneous administration of streptozotocin (100 mg/Kg) in female Wistar rats of 2 days old (STZ,
n
= 10). Control group (CG,
n
= 10) received only sodium-citrate buffer. The mineral concentrations were measured by atomic absorption spectrophotometry. The validity and accuracy were checked by conventional methods. STZ neonatal injection successfully leaded to mild diabetes in the adult rats. Serum concentrations of Zn, Cu, Fe, Ca, and Mg showed no changes (
p
> 0.05) due to diabetes. The Zn, Fe, Ca, and Mg concentrations in liver and kidney tissues were not different (
p
> 0.05) between STZ and CG. The mean values of Cu were higher (
p
< 0.05) in liver and kidney samples from STZ as compared to CG. Urine minerals concentrations (Zn, Cu, Fe and Ca) in STZ-rats group were lower (
p
< 0.05) than CG. However, the content of all evaluated minerals in the excreted urine were higher (
p
< 0.01) in STZ-rats during a 24 h collection period. Urinary excretion of Zn, Cu, Fe, Ca, and Mg was strongly correlated with urinary volume during the 24 h period (
r
> 0.7;
p
< 0.001). Observed changes in mineral metabolism of STZ-induced mild diabetes model could be due to the endocrine imbalance associated with the diabetic condition.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>28258359</pmid><doi>10.1007/s12011-017-0962-x</doi><tpages>10</tpages></addata></record> |
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| ispartof | Biological trace element research, 2017-10, Vol.179 (2), p.237-246 |
| issn | 0163-4984 1559-0720 |
| language | eng |
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| source | SpringerLink Journals |
| subjects | Animal models Atomic absorption analysis Atomic absorption spectrophotometry Biochemistry Biomedical and Life Sciences Biotechnology Calcium Citric acid Copper Diabetes Diabetes mellitus Dietary minerals Iron Kidneys Life Sciences Liver Magnesium Mineral metabolism Minerals Neonates Nutrition Oncology Sodium citrate Spectral analysis Spectrophotometry Streptozocin Urine Zinc |
| title | Concentration of Zinc, Copper, Iron, Calcium, and Magnesium in the Serum, Tissues, and Urine of Streptozotocin-Induced Mild Diabetic Rat Model |
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