Mini-column assay for rapid detection of malachite green in fish
•Optimization of mini-column assay.•Suggested mini-column assay.•Suitability of the assay.•Assessment the assay.•Mini-column and other dyes. A simple, rapid and economical mini-column method for detecting malachite green (MG) residue in fish was developed. The method used a column with 2mm ID that w...
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| Veröffentlicht in: | Food chemistry 2017-07, Vol.226, p.8-13 |
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| creator | Shalaby, Ali R. Emam, Wafaa H. Anwar, Mervat M. |
| description | •Optimization of mini-column assay.•Suggested mini-column assay.•Suitability of the assay.•Assessment the assay.•Mini-column and other dyes.
A simple, rapid and economical mini-column method for detecting malachite green (MG) residue in fish was developed. The method used a column with 2mm ID that was tightly packed with silica gel followed by alumina. Detection of MG was performed by viewing the developed mini-column at visible light by naked eye; where MG was seen as compact green band at the confluence of the silica gel layer with alumina layer. The limit of detection of the assay was 2ng which conform the minimum required performance limit (MRPL). Evaluation utility of the method indicated that all blank and spiked samples at levels below MRPL were assessed as accepted. The intensity of the green band increased whenever MG level in the extract increased; indicated that suggested mini-column technique could be used for semi-quantitative determination of MG in fish samples. The method can be used to select the questionable samples. |
| doi_str_mv | 10.1016/j.foodchem.2017.01.045 |
| format | Article |
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A simple, rapid and economical mini-column method for detecting malachite green (MG) residue in fish was developed. The method used a column with 2mm ID that was tightly packed with silica gel followed by alumina. Detection of MG was performed by viewing the developed mini-column at visible light by naked eye; where MG was seen as compact green band at the confluence of the silica gel layer with alumina layer. The limit of detection of the assay was 2ng which conform the minimum required performance limit (MRPL). Evaluation utility of the method indicated that all blank and spiked samples at levels below MRPL were assessed as accepted. The intensity of the green band increased whenever MG level in the extract increased; indicated that suggested mini-column technique could be used for semi-quantitative determination of MG in fish samples. The method can be used to select the questionable samples.</description><identifier>ISSN: 0308-8146</identifier><identifier>EISSN: 1873-7072</identifier><identifier>DOI: 10.1016/j.foodchem.2017.01.045</identifier><identifier>PMID: 28254023</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Aluminum Oxide ; Chromatography ; Chromatography, Liquid - methods ; Fish ; Fish Products - analysis ; Limit of Detection ; Malachite green ; Mini-column ; Rosaniline Dyes - analysis ; Rosaniline Dyes - isolation & purification ; Silica Gel</subject><ispartof>Food chemistry, 2017-07, Vol.226, p.8-13</ispartof><rights>2017 Elsevier Ltd</rights><rights>Copyright © 2017 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-983a7da4ac9e66cf0734aab2919aed63df19b7bd487dee702f4dc688513dfc2a3</citedby><cites>FETCH-LOGICAL-c405t-983a7da4ac9e66cf0734aab2919aed63df19b7bd487dee702f4dc688513dfc2a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0308814617300456$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28254023$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shalaby, Ali R.</creatorcontrib><creatorcontrib>Emam, Wafaa H.</creatorcontrib><creatorcontrib>Anwar, Mervat M.</creatorcontrib><title>Mini-column assay for rapid detection of malachite green in fish</title><title>Food chemistry</title><addtitle>Food Chem</addtitle><description>•Optimization of mini-column assay.•Suggested mini-column assay.•Suitability of the assay.•Assessment the assay.•Mini-column and other dyes.
A simple, rapid and economical mini-column method for detecting malachite green (MG) residue in fish was developed. The method used a column with 2mm ID that was tightly packed with silica gel followed by alumina. Detection of MG was performed by viewing the developed mini-column at visible light by naked eye; where MG was seen as compact green band at the confluence of the silica gel layer with alumina layer. The limit of detection of the assay was 2ng which conform the minimum required performance limit (MRPL). Evaluation utility of the method indicated that all blank and spiked samples at levels below MRPL were assessed as accepted. The intensity of the green band increased whenever MG level in the extract increased; indicated that suggested mini-column technique could be used for semi-quantitative determination of MG in fish samples. The method can be used to select the questionable samples.</description><subject>Aluminum Oxide</subject><subject>Chromatography</subject><subject>Chromatography, Liquid - methods</subject><subject>Fish</subject><subject>Fish Products - analysis</subject><subject>Limit of Detection</subject><subject>Malachite green</subject><subject>Mini-column</subject><subject>Rosaniline Dyes - analysis</subject><subject>Rosaniline Dyes - isolation & purification</subject><subject>Silica Gel</subject><issn>0308-8146</issn><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1PwzAMhiMEgjH4CyhHLi1OmzXpbQjxJQ1xgXOUJQ7L1DYj6ZD492Ta4IovPvixrfch5IpByYA1N-vShWDNCvuyAiZKYCXw2RGZMCnqQoCojskEapCFZLw5I-cprQEgs_KUnFWymnGo6gmZv_jBFyZ0236gOiX9TV2INOqNt9TiiGb0YaDB0V532qz8iPQjIg7UD9T5tLogJ053CS8PfUreH-7f7p6Kxevj893tojAcZmPRyloLq7k2LTaNcSBqrvWyalmr0Ta1daxdiqXlUlhEAZXj1jRSzlgemUrXU3K9v7uJ4XOLaVS9Twa7Tg8Ytknl3DxX2_KMNnvUxJBSRKc20fc6fisGamdPrdWvPbWzp4CpbC8vXh1-bJc92r-1X10ZmO8BzEm_PEaVjMfBoPUxm1I2-P9-_ABf34Qh</recordid><startdate>20170701</startdate><enddate>20170701</enddate><creator>Shalaby, Ali R.</creator><creator>Emam, Wafaa H.</creator><creator>Anwar, Mervat M.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170701</creationdate><title>Mini-column assay for rapid detection of malachite green in fish</title><author>Shalaby, Ali R. ; Emam, Wafaa H. ; Anwar, Mervat M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-983a7da4ac9e66cf0734aab2919aed63df19b7bd487dee702f4dc688513dfc2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Aluminum Oxide</topic><topic>Chromatography</topic><topic>Chromatography, Liquid - methods</topic><topic>Fish</topic><topic>Fish Products - analysis</topic><topic>Limit of Detection</topic><topic>Malachite green</topic><topic>Mini-column</topic><topic>Rosaniline Dyes - analysis</topic><topic>Rosaniline Dyes - isolation & purification</topic><topic>Silica Gel</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shalaby, Ali R.</creatorcontrib><creatorcontrib>Emam, Wafaa H.</creatorcontrib><creatorcontrib>Anwar, Mervat M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shalaby, Ali R.</au><au>Emam, Wafaa H.</au><au>Anwar, Mervat M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mini-column assay for rapid detection of malachite green in fish</atitle><jtitle>Food chemistry</jtitle><addtitle>Food Chem</addtitle><date>2017-07-01</date><risdate>2017</risdate><volume>226</volume><spage>8</spage><epage>13</epage><pages>8-13</pages><issn>0308-8146</issn><eissn>1873-7072</eissn><abstract>•Optimization of mini-column assay.•Suggested mini-column assay.•Suitability of the assay.•Assessment the assay.•Mini-column and other dyes.
A simple, rapid and economical mini-column method for detecting malachite green (MG) residue in fish was developed. The method used a column with 2mm ID that was tightly packed with silica gel followed by alumina. Detection of MG was performed by viewing the developed mini-column at visible light by naked eye; where MG was seen as compact green band at the confluence of the silica gel layer with alumina layer. The limit of detection of the assay was 2ng which conform the minimum required performance limit (MRPL). Evaluation utility of the method indicated that all blank and spiked samples at levels below MRPL were assessed as accepted. The intensity of the green band increased whenever MG level in the extract increased; indicated that suggested mini-column technique could be used for semi-quantitative determination of MG in fish samples. The method can be used to select the questionable samples.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>28254023</pmid><doi>10.1016/j.foodchem.2017.01.045</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Aluminum Oxide Chromatography Chromatography, Liquid - methods Fish Fish Products - analysis Limit of Detection Malachite green Mini-column Rosaniline Dyes - analysis Rosaniline Dyes - isolation & purification Silica Gel |
| title | Mini-column assay for rapid detection of malachite green in fish |
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