Suppressor of RNA silencing encoded by Beet yellows virus
Using an Agrobacterium-mediated transient assay, we screened the 15.5-kb genome of the Beet yellows virus for proteins with RNA silencing suppressor activity. Among eight proteins tested, only a 21-kDa protein (p21) was able to suppress double-stranded (ds) RNA-induced silencing of the green fluores...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 2003-02, Vol.306 (2), p.203-209 |
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| container_title | Virology (New York, N.Y.) |
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| creator | Reed, Jonathan C Kasschau, Kristin D Prokhnevsky, Alexey I Gopinath, Kodetham Pogue, Gregory P Carrington, James C Dolja, Valerian V |
| description | Using an
Agrobacterium-mediated transient assay, we screened the 15.5-kb genome of the
Beet yellows virus for proteins with RNA silencing suppressor activity. Among eight proteins tested, only a 21-kDa protein (p21) was able to suppress double-stranded (ds) RNA-induced silencing of the green fluorescent protein (GFP) mRNA. Restoration of GFP expression by p21 under these conditions had no apparent effect on accumulation of the small interfering RNAs. In addition, p21 elevated the transient expression level of the GFP mRNA in the absence of dsRNA inducer. Similar activities were detected using homologs of p21 encoded by other members of the genus
Closterovirus. Computer analysis indicated that p21-like proteins constitute a novel protein family that is unrelated to other recognized suppressors of RNA silencing. Examination of the subcellular distribution in BYV-infected plants revealed that p21 is partitioned between soluble cytoplasmic form and proteinaceous inclusion bodies at the cell periphery. |
| doi_str_mv | 10.1016/S0042-6822(02)00051-X |
| format | Article |
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Agrobacterium-mediated transient assay, we screened the 15.5-kb genome of the
Beet yellows virus for proteins with RNA silencing suppressor activity. Among eight proteins tested, only a 21-kDa protein (p21) was able to suppress double-stranded (ds) RNA-induced silencing of the green fluorescent protein (GFP) mRNA. Restoration of GFP expression by p21 under these conditions had no apparent effect on accumulation of the small interfering RNAs. In addition, p21 elevated the transient expression level of the GFP mRNA in the absence of dsRNA inducer. Similar activities were detected using homologs of p21 encoded by other members of the genus
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Agrobacterium-mediated transient assay, we screened the 15.5-kb genome of the
Beet yellows virus for proteins with RNA silencing suppressor activity. Among eight proteins tested, only a 21-kDa protein (p21) was able to suppress double-stranded (ds) RNA-induced silencing of the green fluorescent protein (GFP) mRNA. Restoration of GFP expression by p21 under these conditions had no apparent effect on accumulation of the small interfering RNAs. In addition, p21 elevated the transient expression level of the GFP mRNA in the absence of dsRNA inducer. Similar activities were detected using homologs of p21 encoded by other members of the genus
Closterovirus. Computer analysis indicated that p21-like proteins constitute a novel protein family that is unrelated to other recognized suppressors of RNA silencing. Examination of the subcellular distribution in BYV-infected plants revealed that p21 is partitioned between soluble cytoplasmic form and proteinaceous inclusion bodies at the cell periphery.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Closterovirus - genetics</subject><subject>DNA, Viral - genetics</subject><subject>Green Fluorescent Proteins</subject><subject>Luminescent Proteins - genetics</subject><subject>Molecular Sequence Data</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - metabolism</subject><subject>Nicotiana - virology</subject><subject>Plants, Genetically Modified</subject><subject>Rhizobium - genetics</subject><subject>RNA Interference</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><subject>Suppression, Genetic</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - metabolism</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKAzEUhoMotl4eQZmV6GI0t5lMVlKLNygKtovuQpI5I5FpU5OZSt_eGVt0KRw4m-8_lw-hM4KvCSb5zRRjTtO8oPQS0yuMcUbS-R4aEizzFDNO9tHwFxmgoxg_OogLgQ_RgNCcUyzZEMlpu1oFiNGHxFfJ28soia6GpXXL96RrvoQyMZvkDqBJNlDX_ismaxfaeIIOKl1HON31YzR7uJ-Nn9LJ6-PzeDRJLee8SWVVGGMZy7JMaEqEAEmI1FZQqTXXJhOCmJwwWeSUVkaX1hQl1bhiYEzJ2DG62I5dBf_ZQmzUwkXbHaKX4NuoSCGYxKIHsy1og48xQKVWwS102CiCVa9M_ShTvQ-Fu-qVqXmXO98taM0Cyr_UzlEH3G4B6L5cOwgqWtepgdIFsI0qvftnxTdIR3qv</recordid><startdate>20030215</startdate><enddate>20030215</enddate><creator>Reed, Jonathan C</creator><creator>Kasschau, Kristin D</creator><creator>Prokhnevsky, Alexey I</creator><creator>Gopinath, Kodetham</creator><creator>Pogue, Gregory P</creator><creator>Carrington, James C</creator><creator>Dolja, Valerian V</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20030215</creationdate><title>Suppressor of RNA silencing encoded by Beet yellows virus</title><author>Reed, Jonathan C ; 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Agrobacterium-mediated transient assay, we screened the 15.5-kb genome of the
Beet yellows virus for proteins with RNA silencing suppressor activity. Among eight proteins tested, only a 21-kDa protein (p21) was able to suppress double-stranded (ds) RNA-induced silencing of the green fluorescent protein (GFP) mRNA. Restoration of GFP expression by p21 under these conditions had no apparent effect on accumulation of the small interfering RNAs. In addition, p21 elevated the transient expression level of the GFP mRNA in the absence of dsRNA inducer. Similar activities were detected using homologs of p21 encoded by other members of the genus
Closterovirus. Computer analysis indicated that p21-like proteins constitute a novel protein family that is unrelated to other recognized suppressors of RNA silencing. Examination of the subcellular distribution in BYV-infected plants revealed that p21 is partitioned between soluble cytoplasmic form and proteinaceous inclusion bodies at the cell periphery.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>12642093</pmid><doi>10.1016/S0042-6822(02)00051-X</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Amino Acid Sequence Base Sequence Closterovirus - genetics DNA, Viral - genetics Green Fluorescent Proteins Luminescent Proteins - genetics Molecular Sequence Data Nicotiana - genetics Nicotiana - metabolism Nicotiana - virology Plants, Genetically Modified Rhizobium - genetics RNA Interference RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Homology, Amino Acid Suppression, Genetic Viral Proteins - genetics Viral Proteins - metabolism |
| title | Suppressor of RNA silencing encoded by Beet yellows virus |
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