DAPNe with micro-capillary separatory chemistry-coupled to MALDI-MS for the analysis of polar and non-polar lipid metabolism in one cell
The cellular metabolome is considered to be a representation of cellular phenotype and cellular response to changes to internal or external events. Methods to expand the coverage of the expansive physiochemical properties that makeup the metabolome currently utilize multi-step extractions and chroma...
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| Veröffentlicht in: | Journal of the American Society for Mass Spectrometry 2017-05, Vol.28 (5), p.918-928 |
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| container_title | Journal of the American Society for Mass Spectrometry |
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| creator | Hamilton, Jason S. Aguilar, Roberto Petros, Robby A. Verbeck, Guido F. |
| description | The cellular metabolome is considered to be a representation of cellular phenotype and cellular response to changes to internal or external events. Methods to expand the coverage of the expansive physiochemical properties that makeup the metabolome currently utilize multi-step extractions and chromatographic separations prior to chemical detection, leading to lengthy analysis times. In this study, a single-step procedure for the extraction and separation of a sample using a micro-capillary as a separatory funnel to achieve analyte partitioning within an organic/aqueous immiscible solvent system is described. The separated analytes are then spotted for MALDI-MS imaging and distribution ratios are calculated. Initially, the method is applied to standard mixtures for proof of partitioning. The extraction of an individual cell is non-reproducible; therefore, a broad chemical analysis of metabolites is necessary and will be illustrated with the one-cell analysis of a single Snu-5 gastric cancer cell taken from a cellular suspension. The method presented here shows a broad partitioning dynamic range as a single-step method for lipid analysis demonstrating a decrease in ion suppression often present in MALDI analysis of lipids.
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| doi_str_mv | 10.1007/s13361-017-1623-1 |
| format | Article |
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Graphical Abstract
ᅟ</description><identifier>ISSN: 1044-0305</identifier><identifier>EISSN: 1879-1123</identifier><identifier>DOI: 10.1007/s13361-017-1623-1</identifier><identifier>PMID: 28251574</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Analytical Chemistry ; Bioinformatics ; Biotechnology ; Cell Line, Tumor ; Chemical analysis ; Chemical Fractionation - instrumentation ; Chemistry ; Chemistry and Materials Science ; Dynamic range ; Equipment Design ; Extraction ; Humans ; Lipid Metabolism ; Lipids ; Mass spectrometry ; Metabolism ; Metabolites ; Metabolome ; Metabolomics - instrumentation ; Organic Chemistry ; Partitioning ; Physiochemistry ; Proteomics ; Research Article ; Single-Cell Analysis - instrumentation ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation ; Stomach Neoplasms - metabolism</subject><ispartof>Journal of the American Society for Mass Spectrometry, 2017-05, Vol.28 (5), p.918-928</ispartof><rights>American Society for Mass Spectrometry 2017</rights><rights>Journal of The American Society for Mass Spectrometry is a copyright of Springer, (2017). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-eb521c963b371f1174d1b1ffd0b7ad959aff828d302bf4c52f92f9e31171f3843</citedby><cites>FETCH-LOGICAL-c372t-eb521c963b371f1174d1b1ffd0b7ad959aff828d302bf4c52f92f9e31171f3843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13361-017-1623-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13361-017-1623-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27915,27916,41479,42548,51310</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28251574$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hamilton, Jason S.</creatorcontrib><creatorcontrib>Aguilar, Roberto</creatorcontrib><creatorcontrib>Petros, Robby A.</creatorcontrib><creatorcontrib>Verbeck, Guido F.</creatorcontrib><title>DAPNe with micro-capillary separatory chemistry-coupled to MALDI-MS for the analysis of polar and non-polar lipid metabolism in one cell</title><title>Journal of the American Society for Mass Spectrometry</title><addtitle>J. Am. Soc. Mass Spectrom</addtitle><addtitle>J Am Soc Mass Spectrom</addtitle><description>The cellular metabolome is considered to be a representation of cellular phenotype and cellular response to changes to internal or external events. Methods to expand the coverage of the expansive physiochemical properties that makeup the metabolome currently utilize multi-step extractions and chromatographic separations prior to chemical detection, leading to lengthy analysis times. In this study, a single-step procedure for the extraction and separation of a sample using a micro-capillary as a separatory funnel to achieve analyte partitioning within an organic/aqueous immiscible solvent system is described. The separated analytes are then spotted for MALDI-MS imaging and distribution ratios are calculated. Initially, the method is applied to standard mixtures for proof of partitioning. The extraction of an individual cell is non-reproducible; therefore, a broad chemical analysis of metabolites is necessary and will be illustrated with the one-cell analysis of a single Snu-5 gastric cancer cell taken from a cellular suspension. The method presented here shows a broad partitioning dynamic range as a single-step method for lipid analysis demonstrating a decrease in ion suppression often present in MALDI analysis of lipids.
Graphical Abstract
ᅟ</description><subject>Analytical Chemistry</subject><subject>Bioinformatics</subject><subject>Biotechnology</subject><subject>Cell Line, Tumor</subject><subject>Chemical analysis</subject><subject>Chemical Fractionation - instrumentation</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Dynamic range</subject><subject>Equipment Design</subject><subject>Extraction</subject><subject>Humans</subject><subject>Lipid Metabolism</subject><subject>Lipids</subject><subject>Mass spectrometry</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Metabolome</subject><subject>Metabolomics - instrumentation</subject><subject>Organic Chemistry</subject><subject>Partitioning</subject><subject>Physiochemistry</subject><subject>Proteomics</subject><subject>Research Article</subject><subject>Single-Cell Analysis - instrumentation</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation</subject><subject>Stomach Neoplasms - metabolism</subject><issn>1044-0305</issn><issn>1879-1123</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kd9qFDEUxoMotlYfwBsJeONNbE6S2cxcLm3VwlYF9TpkZhI3JTMZkxlk36CP7VmmihQKgfz75Ts530fIa-DvgXN9XkDKDTAOmsFGSAZPyCnUumEAQj7FNVeKccmrE_KilFuOIG_0c3IialFBpdUpubvcfv3s6O8w7-kQupxYZ6cQo80HWtxks50TLru9G0KZ84F1aZmi6-mc6M12d3nNbr5RnzKd947a0cZDCYUmT6eEGnjS0zGNbN3FMIWeDm62bYqhDDSMNI2Odi7Gl-SZt7G4V_fzGfnx4er7xSe2-_Lx-mK7Y53UYmaurQR0zUa2UoMH0KqHFrzveatt31SN9b4WdS-5aL3qKuEbHE4iCV7WSp6Rd6vulNOvxZXZYGPHD9jRpaUY9A8LCQ6A6NsH6G1aMvaIVCM2qq6V0kjBSqF5pWTnzZTDgP4Z4OYYk1ljMui-OcZkjspv7pWXdnD9vxd_c0FArEDBq_Gny_-VflT1DwI6nS0</recordid><startdate>20170501</startdate><enddate>20170501</enddate><creator>Hamilton, Jason S.</creator><creator>Aguilar, Roberto</creator><creator>Petros, Robby A.</creator><creator>Verbeck, Guido F.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>P5Z</scope><scope>P62</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20170501</creationdate><title>DAPNe with micro-capillary separatory chemistry-coupled to MALDI-MS for the analysis of polar and non-polar lipid metabolism in one cell</title><author>Hamilton, Jason S. ; Aguilar, Roberto ; Petros, Robby A. ; Verbeck, Guido F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-eb521c963b371f1174d1b1ffd0b7ad959aff828d302bf4c52f92f9e31171f3843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Analytical Chemistry</topic><topic>Bioinformatics</topic><topic>Biotechnology</topic><topic>Cell Line, Tumor</topic><topic>Chemical analysis</topic><topic>Chemical Fractionation - instrumentation</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Dynamic range</topic><topic>Equipment Design</topic><topic>Extraction</topic><topic>Humans</topic><topic>Lipid Metabolism</topic><topic>Lipids</topic><topic>Mass spectrometry</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Metabolome</topic><topic>Metabolomics - instrumentation</topic><topic>Organic Chemistry</topic><topic>Partitioning</topic><topic>Physiochemistry</topic><topic>Proteomics</topic><topic>Research Article</topic><topic>Single-Cell Analysis - instrumentation</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation</topic><topic>Stomach Neoplasms - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hamilton, Jason S.</creatorcontrib><creatorcontrib>Aguilar, Roberto</creatorcontrib><creatorcontrib>Petros, Robby A.</creatorcontrib><creatorcontrib>Verbeck, Guido F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the American Society for Mass Spectrometry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hamilton, Jason S.</au><au>Aguilar, Roberto</au><au>Petros, Robby A.</au><au>Verbeck, Guido F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DAPNe with micro-capillary separatory chemistry-coupled to MALDI-MS for the analysis of polar and non-polar lipid metabolism in one cell</atitle><jtitle>Journal of the American Society for Mass Spectrometry</jtitle><stitle>J. Am. Soc. Mass Spectrom</stitle><addtitle>J Am Soc Mass Spectrom</addtitle><date>2017-05-01</date><risdate>2017</risdate><volume>28</volume><issue>5</issue><spage>918</spage><epage>928</epage><pages>918-928</pages><issn>1044-0305</issn><eissn>1879-1123</eissn><abstract>The cellular metabolome is considered to be a representation of cellular phenotype and cellular response to changes to internal or external events. Methods to expand the coverage of the expansive physiochemical properties that makeup the metabolome currently utilize multi-step extractions and chromatographic separations prior to chemical detection, leading to lengthy analysis times. In this study, a single-step procedure for the extraction and separation of a sample using a micro-capillary as a separatory funnel to achieve analyte partitioning within an organic/aqueous immiscible solvent system is described. The separated analytes are then spotted for MALDI-MS imaging and distribution ratios are calculated. Initially, the method is applied to standard mixtures for proof of partitioning. The extraction of an individual cell is non-reproducible; therefore, a broad chemical analysis of metabolites is necessary and will be illustrated with the one-cell analysis of a single Snu-5 gastric cancer cell taken from a cellular suspension. The method presented here shows a broad partitioning dynamic range as a single-step method for lipid analysis demonstrating a decrease in ion suppression often present in MALDI analysis of lipids.
Graphical Abstract
ᅟ</abstract><cop>New York</cop><pub>Springer US</pub><pmid>28251574</pmid><doi>10.1007/s13361-017-1623-1</doi><tpages>11</tpages></addata></record> |
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| ispartof | Journal of the American Society for Mass Spectrometry, 2017-05, Vol.28 (5), p.918-928 |
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| subjects | Analytical Chemistry Bioinformatics Biotechnology Cell Line, Tumor Chemical analysis Chemical Fractionation - instrumentation Chemistry Chemistry and Materials Science Dynamic range Equipment Design Extraction Humans Lipid Metabolism Lipids Mass spectrometry Metabolism Metabolites Metabolome Metabolomics - instrumentation Organic Chemistry Partitioning Physiochemistry Proteomics Research Article Single-Cell Analysis - instrumentation Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation Stomach Neoplasms - metabolism |
| title | DAPNe with micro-capillary separatory chemistry-coupled to MALDI-MS for the analysis of polar and non-polar lipid metabolism in one cell |
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