The Riboswitch-Mediated Control of Sulfur Metabolism in Bacteria
Many operons in Gram-positive bacteria that are involved in methionine (Met) and cysteine (Cys) biosynthesis possess an evolutionarily conserved regulatory leader sequence (S-box) that positively controls these genes in response to methionine starvation. Here, we demonstrate that a feed-back regulat...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2003-04, Vol.100 (9), p.5052-5056 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Epshtein, Vitaly Mironov, Alexander S. Nudler, Evgeny |
| description | Many operons in Gram-positive bacteria that are involved in methionine (Met) and cysteine (Cys) biosynthesis possess an evolutionarily conserved regulatory leader sequence (S-box) that positively controls these genes in response to methionine starvation. Here, we demonstrate that a feed-back regulation mechanism utilizes S-adenosyl-methionine as an effector. S-adenosyl-methionine directly and specifically binds to the nascent S-box RNA, causing an intrinsic terminator to form and interrupt transcription prematurely. The S-box leader RNA thus expands the family of newly discovered riboswitches, i.e., natural regulatory RNA aptamers that seem to sense small molecules ranging from amino acid derivatives to vitamins. |
| doi_str_mv | 10.1073/pnas.0531307100 |
| format | Article |
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Here, we demonstrate that a feed-back regulation mechanism utilizes S-adenosyl-methionine as an effector. S-adenosyl-methionine directly and specifically binds to the nascent S-box RNA, causing an intrinsic terminator to form and interrupt transcription prematurely. 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Here, we demonstrate that a feed-back regulation mechanism utilizes S-adenosyl-methionine as an effector. S-adenosyl-methionine directly and specifically binds to the nascent S-box RNA, causing an intrinsic terminator to form and interrupt transcription prematurely. The S-box leader RNA thus expands the family of newly discovered riboswitches, i.e., natural regulatory RNA aptamers that seem to sense small molecules ranging from amino acid derivatives to vitamins.</description><subject>Bacteria</subject><subject>Base Sequence</subject><subject>Biological Sciences</subject><subject>Biosynthesis</subject><subject>DNA</subject><subject>Gene expression regulation</subject><subject>Genes</subject><subject>Genetic mutation</subject><subject>Gram-Positive Bacteria - metabolism</subject><subject>Metabolism</subject><subject>Models, Molecular</subject><subject>Molecular Sequence Data</subject><subject>Molecules</subject><subject>Operon</subject><subject>Operons</subject><subject>RNA</subject><subject>RNA, Bacterial - metabolism</subject><subject>Sulfur</subject><subject>Sulfur - metabolism</subject><subject>Terminator Regions, Genetic</subject><subject>Transcription, Genetic</subject><subject>Transcriptional regulatory elements</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2LFDEQxYMo7rh69iLSeNBT71aSTtI5COrgF-wi6HoO6XS1k6GnM5uk_fjvzTDDziqCpxDq9x6v6hHymMIZBcXPt5NNZyA45aAowB2yoKBpLRsNd8kCgKm6bVhzQh6ktAYALVq4T04oU2Uk1YK8ulph9dl3If3w2a3qS-y9zdhXyzDlGMYqDNWXeRzmWF1itl0YfdpUfqreWJcxevuQ3BvsmPDR4T0lX9-9vVp-qC8-vf-4fH1RO8FYrqkDhkwPkvedRtBSu7aXaNFyq5mlMEjn2rYrPy55M4BiFAQyYRuldMf5KXm5993O3QZ7hyWeHc02-o2Nv0yw3vw5mfzKfAvfDRUN07Lonx_0MVzPmLLZ-ORwHO2EYU5GcSZBaPgvSFvFpWI78Nlf4DrMcSpHMAxoU46tdYHO95CLIaWIw01iCmZXodlVaI4VFsXT24se-UNnBXhxAHbKox0YbQQIZoZ5HDP-zLes_k0W4MkeWKcc4g1RsmgpOf8NHd-3yg</recordid><startdate>20030429</startdate><enddate>20030429</enddate><creator>Epshtein, Vitaly</creator><creator>Mironov, Alexander S.</creator><creator>Nudler, Evgeny</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><general>The National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20030429</creationdate><title>The Riboswitch-Mediated Control of Sulfur Metabolism in Bacteria</title><author>Epshtein, Vitaly ; 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| subjects | Bacteria Base Sequence Biological Sciences Biosynthesis DNA Gene expression regulation Genes Genetic mutation Gram-Positive Bacteria - metabolism Metabolism Models, Molecular Molecular Sequence Data Molecules Operon Operons RNA RNA, Bacterial - metabolism Sulfur Sulfur - metabolism Terminator Regions, Genetic Transcription, Genetic Transcriptional regulatory elements |
| title | The Riboswitch-Mediated Control of Sulfur Metabolism in Bacteria |
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