Activation of Archaeal Transcription by Recruitment of the TATA-Binding Protein
The hyperthermophilic archaeon Methanococcus jannaschii encodes two putative transcription regulators, Ptr1 and Ptr2, that are members of the Lrp/AsnC family of bacterial transcription regulators. In contrast, this archaeon's RNA polymerase and core transcription factors are of eukaryotic type....
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2003-04, Vol.100 (9), p.5097-5102 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Ouhammouch, Mohamed Dewhurst, Robert E. Hausner, Winfried Thomm, Michael Geiduschek, E. Peter |
| description | The hyperthermophilic archaeon Methanococcus jannaschii encodes two putative transcription regulators, Ptr1 and Ptr2, that are members of the Lrp/AsnC family of bacterial transcription regulators. In contrast, this archaeon's RNA polymerase and core transcription factors are of eukaryotic type. Using the M. jannaschii high-temperature in vitro transcription system, we show that Ptr2 is a potent transcriptional activator, and that it conveys its stimulatory effects on its cognate eukaryal-type transcription machinery from an upstream activating region composed of two Ptr2-binding sites. Transcriptional activation is generated, at least in part, by Ptr2-mediated recruitment of the TATA-binding protein to the promoter. |
| doi_str_mv | 10.1073/pnas.0837150100 |
| format | Article |
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Transcriptional activation is generated, at least in part, by Ptr2-mediated recruitment of the TATA-binding protein to the promoter.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.0837150100</identifier><identifier>PMID: 12692306</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Archaea ; Bacteria ; Base Sequence ; Binding sites ; Biological Sciences ; DNA ; DNA Primers ; Hydroxyl Radical ; Intergenic DNA ; Methanococcus - genetics ; Methanococcus - metabolism ; Promoter Regions, Genetic ; Proteins ; Regulator genes ; Ribonucleic acid ; RNA ; TATA box ; TATA-Box Binding Protein - physiology ; Transcription factors ; Transcriptional activation ; Transcriptional Activation - physiology ; Transcriptional regulatory elements</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2003-04, Vol.100 (9), p.5097-5102</ispartof><rights>Copyright 1993-2003 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Apr 29, 2003</rights><rights>Copyright © 2003, The National Academy of Sciences 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c588t-7c6e4a9aa7506ead39ea50c336c1581c8d0e3f7736bd63b6ed8f5548ae7dd5bd3</citedby><cites>FETCH-LOGICAL-c588t-7c6e4a9aa7506ead39ea50c336c1581c8d0e3f7736bd63b6ed8f5548ae7dd5bd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/100/9.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3139671$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3139671$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,724,777,781,800,882,27905,27906,53772,53774,57998,58231</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12692306$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ouhammouch, Mohamed</creatorcontrib><creatorcontrib>Dewhurst, Robert E.</creatorcontrib><creatorcontrib>Hausner, Winfried</creatorcontrib><creatorcontrib>Thomm, Michael</creatorcontrib><creatorcontrib>Geiduschek, E. Peter</creatorcontrib><title>Activation of Archaeal Transcription by Recruitment of the TATA-Binding Protein</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The hyperthermophilic archaeon Methanococcus jannaschii encodes two putative transcription regulators, Ptr1 and Ptr2, that are members of the Lrp/AsnC family of bacterial transcription regulators. In contrast, this archaeon's RNA polymerase and core transcription factors are of eukaryotic type. Using the M. jannaschii high-temperature in vitro transcription system, we show that Ptr2 is a potent transcriptional activator, and that it conveys its stimulatory effects on its cognate eukaryal-type transcription machinery from an upstream activating region composed of two Ptr2-binding sites. Transcriptional activation is generated, at least in part, by Ptr2-mediated recruitment of the TATA-binding protein to the promoter.</description><subject>Archaea</subject><subject>Bacteria</subject><subject>Base Sequence</subject><subject>Binding sites</subject><subject>Biological Sciences</subject><subject>DNA</subject><subject>DNA Primers</subject><subject>Hydroxyl Radical</subject><subject>Intergenic DNA</subject><subject>Methanococcus - genetics</subject><subject>Methanococcus - metabolism</subject><subject>Promoter Regions, Genetic</subject><subject>Proteins</subject><subject>Regulator genes</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>TATA box</subject><subject>TATA-Box Binding Protein - physiology</subject><subject>Transcription factors</subject><subject>Transcriptional activation</subject><subject>Transcriptional Activation - physiology</subject><subject>Transcriptional regulatory elements</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS0EokvhzAWhiAOc0o4z8UcOPYSKL6lSEVrOltdxul5l7cV2Kvrfk7CrbuEApznM7828mUfISwpnFASe77xOZyBRUAYU4BFZUGhoyesGHpMFQCVKWVf1CXmW0gYAGibhKTmhFW8qBL4g163J7lZnF3wR-qKNZq2tHopl1D6Z6Ha_O6u74ps1cXR5a32ewby2xbJdtuV75zvnb4qvMWTr_HPypNdDsi8O9ZR8__hhefm5vLr-9OWyvSoNkzKXwnBb60ZrwYBb3WFjNQODyA1lkhrZgcVeCOSrjuOK2072jNVSW9F1bNXhKbnYz92Nq63tzGQr6kHtotvqeKeCdurPjndrdRNuFWU1Qj3p3x70MfwYbcpq65Kxw6C9DWNSAivkjMJ_QSonk4BiAt_8BW7CGP30BFUBRaQVm9ee7yETQ0rR9veOKag5UTUnqo6JTorXDw898ocIHwCz8jgOVKMYNLOxd_8EVD8OQ7Y_80S-2pOblEO8R5FiwwXFX_O6vjk</recordid><startdate>20030429</startdate><enddate>20030429</enddate><creator>Ouhammouch, Mohamed</creator><creator>Dewhurst, Robert E.</creator><creator>Hausner, Winfried</creator><creator>Thomm, Michael</creator><creator>Geiduschek, E. 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| subjects | Archaea Bacteria Base Sequence Binding sites Biological Sciences DNA DNA Primers Hydroxyl Radical Intergenic DNA Methanococcus - genetics Methanococcus - metabolism Promoter Regions, Genetic Proteins Regulator genes Ribonucleic acid RNA TATA box TATA-Box Binding Protein - physiology Transcription factors Transcriptional activation Transcriptional Activation - physiology Transcriptional regulatory elements |
| title | Activation of Archaeal Transcription by Recruitment of the TATA-Binding Protein |
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