Serine 774 and amino acids 296 to 437 comprise the major C4 determinants of the C4 phosphoenolpyruvate carboxylase of Flaveria trinervia
C4 phosphoenolpyruvate carboxylases have evolved several times independently from non‐photosynthetic C3 ancestral enzymes. To identify C4‐specific determinants at the amino acid level the two orthologous ppcA PEPCases of Flaveria trinervia (C4) and Flaveria pringlei (C3) were used as a model system....
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| Veröffentlicht in: | FEBS letters 2002-07, Vol.524 (1-3), p.11-14 |
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| creator | Engelmann, Sascha Bläsing, Oliver E Westhoff, Peter Svensson, Per |
| description | C4 phosphoenolpyruvate carboxylases have evolved several times independently from non‐photosynthetic C3 ancestral enzymes. To identify C4‐specific determinants at the amino acid level the two orthologous ppcA PEPCases of Flaveria trinervia (C4) and Flaveria pringlei (C3) were used as a model system. In a previous publication [Bläsing et al., J. Biol. Chem. 275 (2000) 27917–27923] it was reported that the serine at position 774 is an invariant residue in all C4 phosphoenolpyruvate carboxylases. Here we show by swapping experiments and site‐directed mutagenesis that the serine 774 and amino acids 296–437 explain two thirds of the C4 characteristic phosphoenolpyruvate saturation kinetics when investigated in the C3 background. In addition, the results indicate that the determinants functionally interact with each other. |
| doi_str_mv | 10.1016/S0014-5793(02)02975-7 |
| format | Article |
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To identify C4‐specific determinants at the amino acid level the two orthologous ppcA PEPCases of Flaveria trinervia (C4) and Flaveria pringlei (C3) were used as a model system. In a previous publication [Bläsing et al., J. Biol. Chem. 275 (2000) 27917–27923] it was reported that the serine at position 774 is an invariant residue in all C4 phosphoenolpyruvate carboxylases. Here we show by swapping experiments and site‐directed mutagenesis that the serine 774 and amino acids 296–437 explain two thirds of the C4 characteristic phosphoenolpyruvate saturation kinetics when investigated in the C3 background. In addition, the results indicate that the determinants functionally interact with each other.</description><subject>Amino Acid Sequence</subject><subject>Amino Acids - metabolism</subject><subject>Asteraceae - enzymology</subject><subject>C4 photosynthesis</subject><subject>Evolution</subject><subject>Flaveria</subject><subject>Glc6P, glucose 6-phosphate</subject><subject>Kinetics</subject><subject>Molecular Sequence Data</subject><subject>PEPCase, phosphoenolpyruvate carboxylase</subject><subject>Phosphoenolpyruvate carboxylase</subject><subject>Phosphoenolpyruvate Carboxylase - chemistry</subject><subject>Phosphoenolpyruvate Carboxylase - metabolism</subject><subject>ppc, phosphoenolpyruvate carboxylase gene</subject><subject>Sequence Homology, Amino Acid</subject><subject>Serine - metabolism</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkc9O3DAQxq2qqGyXPkIrnyp6SPHfODnSFVsqIXEAztZsPBZBSby1s0v3DXhsnEDhYFme7-cZzfcR8pWzn5zx8uyGMa4KbWp5ysQPJmqjC_OBLHhlZCFVWX0kizfkmHxO6YHld8XrT-SYCy61kXJBnm4wtgNSYxSFwVHo2yFQaFqXqKhLOgaqpKFN6LexTUjHe6Q9PIRIV4o6HDHmDzCMiQY_i7m8vQ8pHxxCtz3E3R5GpA3ETfh36CD3yOS6g30eDHScpsd9CyfkyEOX8MvrvSR364vb1WVxdf37z-r8qmik1qaQXAnn3UZ7rpqmrITXFZh6A1Kj9o4z7QRDVZdMoDdY-tI13IOvgHmHqOSSfH_pu43h7w7TaPs2Ndh1MGDYJTv5x8vs2ZJ8ewV3mx6dzfv3EA_2v3cZuHwBHtsOD-86s1NAdg7ITu5bJuwckDV2ffFLzMokMDGXjXwGV_mGnA</recordid><startdate>20020731</startdate><enddate>20020731</enddate><creator>Engelmann, Sascha</creator><creator>Bläsing, Oliver E</creator><creator>Westhoff, Peter</creator><creator>Svensson, Per</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20020731</creationdate><title>Serine 774 and amino acids 296 to 437 comprise the major C4 determinants of the C4 phosphoenolpyruvate carboxylase of Flaveria trinervia</title><author>Engelmann, Sascha ; Bläsing, Oliver E ; Westhoff, Peter ; Svensson, Per</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3557-3142dfdb5f14cc682f58a79ba35e5fd105d20e49602ef7e6f6dc1faf8a0fdee43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Amino Acid Sequence</topic><topic>Amino Acids - metabolism</topic><topic>Asteraceae - enzymology</topic><topic>C4 photosynthesis</topic><topic>Evolution</topic><topic>Flaveria</topic><topic>Glc6P, glucose 6-phosphate</topic><topic>Kinetics</topic><topic>Molecular Sequence Data</topic><topic>PEPCase, phosphoenolpyruvate carboxylase</topic><topic>Phosphoenolpyruvate carboxylase</topic><topic>Phosphoenolpyruvate Carboxylase - chemistry</topic><topic>Phosphoenolpyruvate Carboxylase - metabolism</topic><topic>ppc, phosphoenolpyruvate carboxylase gene</topic><topic>Sequence Homology, Amino Acid</topic><topic>Serine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Engelmann, Sascha</creatorcontrib><creatorcontrib>Bläsing, Oliver E</creatorcontrib><creatorcontrib>Westhoff, Peter</creatorcontrib><creatorcontrib>Svensson, Per</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Engelmann, Sascha</au><au>Bläsing, Oliver E</au><au>Westhoff, Peter</au><au>Svensson, Per</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serine 774 and amino acids 296 to 437 comprise the major C4 determinants of the C4 phosphoenolpyruvate carboxylase of Flaveria trinervia</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2002-07-31</date><risdate>2002</risdate><volume>524</volume><issue>1-3</issue><spage>11</spage><epage>14</epage><pages>11-14</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>C4 phosphoenolpyruvate carboxylases have evolved several times independently from non‐photosynthetic C3 ancestral enzymes. 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| ispartof | FEBS letters, 2002-07, Vol.524 (1-3), p.11-14 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Amino Acids - metabolism Asteraceae - enzymology C4 photosynthesis Evolution Flaveria Glc6P, glucose 6-phosphate Kinetics Molecular Sequence Data PEPCase, phosphoenolpyruvate carboxylase Phosphoenolpyruvate carboxylase Phosphoenolpyruvate Carboxylase - chemistry Phosphoenolpyruvate Carboxylase - metabolism ppc, phosphoenolpyruvate carboxylase gene Sequence Homology, Amino Acid Serine - metabolism |
| title | Serine 774 and amino acids 296 to 437 comprise the major C4 determinants of the C4 phosphoenolpyruvate carboxylase of Flaveria trinervia |
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