Biochemical response of hybrid black poplar tissue culture (Populus × canadensis) on water stress
In this study, poplar tissue culture (hybrid black poplar, M1 genotype) was subjected to water stress influenced by polyethyleneglycol 6000 (100 and 200 mOsm PEG 6000). The aim of the research was to investigate the biochemical response of poplar tissue culture on water deficit regime. Antioxidant s...
Gespeichert in:
| Veröffentlicht in: | Journal of plant research 2017-05, Vol.130 (3), p.559-570 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 570 |
|---|---|
| container_issue | 3 |
| container_start_page | 559 |
| container_title | Journal of plant research |
| container_volume | 130 |
| creator | Popović, B. M. Štajner, D. Ždero-Pavlović, R. Tari, I. Csiszár, J. Gallé, Á. Poór, P. Galović, V. Trudić, B. Orlović, S. |
| description | In this study, poplar tissue culture (hybrid black poplar, M1 genotype) was subjected to water stress influenced by polyethyleneglycol 6000 (100 and 200 mOsm PEG 6000). The aim of the research was to investigate the biochemical response of poplar tissue culture on water deficit regime. Antioxidant status was analyzed including antioxidant enzymes, superoxide-dismutase (SOD), catalase (CAT), guiacol-peroxidase (GPx), glutathione-peroxidase (GSH-Px), glutathione-reductase, reduced glutathione, total phenol content, Ferric reducing antioxidant power and DPPH radical antioxidant power. Polyphenol oxidase and phenylalanine-ammonium-lyase were determined as enzymatic markers of polyphenol metabolism. Among oxidative stress parameters lipid peroxidation, carbonyl-proteins, hydrogen-peroxide, reactive oxygen species, nitric-oxide and peroxynitrite were determined. Proline, proline-dehydrogenase and glycinebetaine were measured also as parameters of water stress. Cell viability is finally determined as a biological indicator of osmotic stress. It was found that water stress induced reactive oxygen and nitrogen species and lipid peroxidation in leaves of hybrid black poplar and reduced cell viability. Antioxidant enzymes including SOD, GPx, CAT and GSH-Px were induced but total phenol content and antioxidant capacity were reduced by PEG 6000 mediated osmotic stress. The highest biochemical response and adaptive reaction was the increase of proline and GB especially by 200 mOsm PEG. While long term molecular analysis will be necessary to fully address the poplar potentials for water stress adaptation, our results on hybrid black poplar suggest that glycine-betaine, proline and PDH enzyme might be the most important markers of poplar on water stress and that future efforts should be focused on these markers and strategies to enhance their concentration in poplar. |
| doi_str_mv | 10.1007/s10265-017-0918-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1872876903</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1872876903</sourcerecordid><originalsourceid>FETCH-LOGICAL-c399t-2b292abebbfb5e9f41f3edcb07ef75b794e42277b9320ae81ca742b7a81f441e3</originalsourceid><addsrcrecordid>eNp1kcFOHDEMhqOKqiy0D8ClitQLPUxJMplNciyIAhJSe6DnKMl4ysDsZBpPhHgSrjwLfbFm2W1VVeJky_782_pNyAFnnzhj6gg5E8umYlxVzHBdyVdkwZclYXrJdsjiuWikZLtkD_GGFbAx-g3ZFVrIWtd8QdrjPoZrWPXBDTQBTnFEoLGj1_c-9S31gwu3dIrT4BKde8QMNORhzgno4bc45SHj0-Ovh6fH4EbXwog9fqRxpHduhkRxLpr4lrzu3IDwbhv3yfcvp1cn59Xl17OLk8-XVaiNmSvhhRHOg_edb8B0knc1tMEzBZ1qvDISpBBKeVML5kDz4JQUXjnNOyk51PvkcKM7pfgzA8521WOAYXAjxIyWayW0WhpWF_TDf-hNzGks1xXKCC4aVZtC8Q0VUkRM0Nkp9SuX7i1ndv0Cu3mBLc7atdtWlpn3W-XsV9D-nfjjeQHEBsDSGn9A-mf1i6q_AQkFlEw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1892125739</pqid></control><display><type>article</type><title>Biochemical response of hybrid black poplar tissue culture (Populus × canadensis) on water stress</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Popović, B. M. ; Štajner, D. ; Ždero-Pavlović, R. ; Tari, I. ; Csiszár, J. ; Gallé, Á. ; Poór, P. ; Galović, V. ; Trudić, B. ; Orlović, S.</creator><creatorcontrib>Popović, B. M. ; Štajner, D. ; Ždero-Pavlović, R. ; Tari, I. ; Csiszár, J. ; Gallé, Á. ; Poór, P. ; Galović, V. ; Trudić, B. ; Orlović, S.</creatorcontrib><description>In this study, poplar tissue culture (hybrid black poplar, M1 genotype) was subjected to water stress influenced by polyethyleneglycol 6000 (100 and 200 mOsm PEG 6000). The aim of the research was to investigate the biochemical response of poplar tissue culture on water deficit regime. Antioxidant status was analyzed including antioxidant enzymes, superoxide-dismutase (SOD), catalase (CAT), guiacol-peroxidase (GPx), glutathione-peroxidase (GSH-Px), glutathione-reductase, reduced glutathione, total phenol content, Ferric reducing antioxidant power and DPPH radical antioxidant power. Polyphenol oxidase and phenylalanine-ammonium-lyase were determined as enzymatic markers of polyphenol metabolism. Among oxidative stress parameters lipid peroxidation, carbonyl-proteins, hydrogen-peroxide, reactive oxygen species, nitric-oxide and peroxynitrite were determined. Proline, proline-dehydrogenase and glycinebetaine were measured also as parameters of water stress. Cell viability is finally determined as a biological indicator of osmotic stress. It was found that water stress induced reactive oxygen and nitrogen species and lipid peroxidation in leaves of hybrid black poplar and reduced cell viability. Antioxidant enzymes including SOD, GPx, CAT and GSH-Px were induced but total phenol content and antioxidant capacity were reduced by PEG 6000 mediated osmotic stress. The highest biochemical response and adaptive reaction was the increase of proline and GB especially by 200 mOsm PEG. While long term molecular analysis will be necessary to fully address the poplar potentials for water stress adaptation, our results on hybrid black poplar suggest that glycine-betaine, proline and PDH enzyme might be the most important markers of poplar on water stress and that future efforts should be focused on these markers and strategies to enhance their concentration in poplar.</description><identifier>ISSN: 0918-9440</identifier><identifier>EISSN: 1618-0860</identifier><identifier>DOI: 10.1007/s10265-017-0918-4</identifier><identifier>PMID: 28243831</identifier><language>eng</language><publisher>Tokyo: Springer Japan</publisher><subject>Ammonium ; Antioxidants ; Antioxidants - analysis ; Betaine - metabolism ; Biochemical Phenomena ; Biomarkers ; Biomedical and Life Sciences ; Carbonyl compounds ; Catalase - metabolism ; Catechol Oxidase - metabolism ; Cell Survival ; Dehydration ; Enzyme Assays ; Enzymes - metabolism ; Glutathione - metabolism ; Glutathione Reductase - metabolism ; Hydrogen Peroxide - metabolism ; Indicator species ; Life Sciences ; Lipid Peroxidation ; Nitric Oxide - metabolism ; Osmotic Pressure ; Oxidative Stress ; Oxidoreductases - metabolism ; Peroxidases - metabolism ; Peroxidation ; Peroxynitrous Acid - metabolism ; Phenol - metabolism ; Phenols ; Plant Biochemistry ; Plant Ecology ; Plant Extracts - chemistry ; Plant Extracts - metabolism ; Plant Leaves - chemistry ; Plant Leaves - metabolism ; Plant Physiology ; Plant Sciences ; Populus - chemistry ; Populus - enzymology ; Populus - metabolism ; Proline - metabolism ; Reactive Nitrogen Species - metabolism ; Reactive Oxygen Species - metabolism ; Regular Paper ; Superoxide Dismutase - metabolism ; Tissue Culture Techniques ; Water - metabolism ; Water deficit ; Water stress</subject><ispartof>Journal of plant research, 2017-05, Vol.130 (3), p.559-570</ispartof><rights>The Botanical Society of Japan and Springer Japan 2017</rights><rights>Journal of Plant Research is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-2b292abebbfb5e9f41f3edcb07ef75b794e42277b9320ae81ca742b7a81f441e3</citedby><cites>FETCH-LOGICAL-c399t-2b292abebbfb5e9f41f3edcb07ef75b794e42277b9320ae81ca742b7a81f441e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10265-017-0918-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10265-017-0918-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28243831$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Popović, B. M.</creatorcontrib><creatorcontrib>Štajner, D.</creatorcontrib><creatorcontrib>Ždero-Pavlović, R.</creatorcontrib><creatorcontrib>Tari, I.</creatorcontrib><creatorcontrib>Csiszár, J.</creatorcontrib><creatorcontrib>Gallé, Á.</creatorcontrib><creatorcontrib>Poór, P.</creatorcontrib><creatorcontrib>Galović, V.</creatorcontrib><creatorcontrib>Trudić, B.</creatorcontrib><creatorcontrib>Orlović, S.</creatorcontrib><title>Biochemical response of hybrid black poplar tissue culture (Populus × canadensis) on water stress</title><title>Journal of plant research</title><addtitle>J Plant Res</addtitle><addtitle>J Plant Res</addtitle><description>In this study, poplar tissue culture (hybrid black poplar, M1 genotype) was subjected to water stress influenced by polyethyleneglycol 6000 (100 and 200 mOsm PEG 6000). The aim of the research was to investigate the biochemical response of poplar tissue culture on water deficit regime. Antioxidant status was analyzed including antioxidant enzymes, superoxide-dismutase (SOD), catalase (CAT), guiacol-peroxidase (GPx), glutathione-peroxidase (GSH-Px), glutathione-reductase, reduced glutathione, total phenol content, Ferric reducing antioxidant power and DPPH radical antioxidant power. Polyphenol oxidase and phenylalanine-ammonium-lyase were determined as enzymatic markers of polyphenol metabolism. Among oxidative stress parameters lipid peroxidation, carbonyl-proteins, hydrogen-peroxide, reactive oxygen species, nitric-oxide and peroxynitrite were determined. Proline, proline-dehydrogenase and glycinebetaine were measured also as parameters of water stress. Cell viability is finally determined as a biological indicator of osmotic stress. It was found that water stress induced reactive oxygen and nitrogen species and lipid peroxidation in leaves of hybrid black poplar and reduced cell viability. Antioxidant enzymes including SOD, GPx, CAT and GSH-Px were induced but total phenol content and antioxidant capacity were reduced by PEG 6000 mediated osmotic stress. The highest biochemical response and adaptive reaction was the increase of proline and GB especially by 200 mOsm PEG. While long term molecular analysis will be necessary to fully address the poplar potentials for water stress adaptation, our results on hybrid black poplar suggest that glycine-betaine, proline and PDH enzyme might be the most important markers of poplar on water stress and that future efforts should be focused on these markers and strategies to enhance their concentration in poplar.</description><subject>Ammonium</subject><subject>Antioxidants</subject><subject>Antioxidants - analysis</subject><subject>Betaine - metabolism</subject><subject>Biochemical Phenomena</subject><subject>Biomarkers</subject><subject>Biomedical and Life Sciences</subject><subject>Carbonyl compounds</subject><subject>Catalase - metabolism</subject><subject>Catechol Oxidase - metabolism</subject><subject>Cell Survival</subject><subject>Dehydration</subject><subject>Enzyme Assays</subject><subject>Enzymes - metabolism</subject><subject>Glutathione - metabolism</subject><subject>Glutathione Reductase - metabolism</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Indicator species</subject><subject>Life Sciences</subject><subject>Lipid Peroxidation</subject><subject>Nitric Oxide - metabolism</subject><subject>Osmotic Pressure</subject><subject>Oxidative Stress</subject><subject>Oxidoreductases - metabolism</subject><subject>Peroxidases - metabolism</subject><subject>Peroxidation</subject><subject>Peroxynitrous Acid - metabolism</subject><subject>Phenol - metabolism</subject><subject>Phenols</subject><subject>Plant Biochemistry</subject><subject>Plant Ecology</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - metabolism</subject><subject>Plant Leaves - chemistry</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Populus - chemistry</subject><subject>Populus - enzymology</subject><subject>Populus - metabolism</subject><subject>Proline - metabolism</subject><subject>Reactive Nitrogen Species - metabolism</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Regular Paper</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Tissue Culture Techniques</subject><subject>Water - metabolism</subject><subject>Water deficit</subject><subject>Water stress</subject><issn>0918-9440</issn><issn>1618-0860</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kcFOHDEMhqOKqiy0D8ClitQLPUxJMplNciyIAhJSe6DnKMl4ysDsZBpPhHgSrjwLfbFm2W1VVeJky_782_pNyAFnnzhj6gg5E8umYlxVzHBdyVdkwZclYXrJdsjiuWikZLtkD_GGFbAx-g3ZFVrIWtd8QdrjPoZrWPXBDTQBTnFEoLGj1_c-9S31gwu3dIrT4BKde8QMNORhzgno4bc45SHj0-Ovh6fH4EbXwog9fqRxpHduhkRxLpr4lrzu3IDwbhv3yfcvp1cn59Xl17OLk8-XVaiNmSvhhRHOg_edb8B0knc1tMEzBZ1qvDISpBBKeVML5kDz4JQUXjnNOyk51PvkcKM7pfgzA8521WOAYXAjxIyWayW0WhpWF_TDf-hNzGks1xXKCC4aVZtC8Q0VUkRM0Nkp9SuX7i1ndv0Cu3mBLc7atdtWlpn3W-XsV9D-nfjjeQHEBsDSGn9A-mf1i6q_AQkFlEw</recordid><startdate>20170501</startdate><enddate>20170501</enddate><creator>Popović, B. M.</creator><creator>Štajner, D.</creator><creator>Ždero-Pavlović, R.</creator><creator>Tari, I.</creator><creator>Csiszár, J.</creator><creator>Gallé, Á.</creator><creator>Poór, P.</creator><creator>Galović, V.</creator><creator>Trudić, B.</creator><creator>Orlović, S.</creator><general>Springer Japan</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7ST</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>20170501</creationdate><title>Biochemical response of hybrid black poplar tissue culture (Populus × canadensis) on water stress</title><author>Popović, B. M. ; Štajner, D. ; Ždero-Pavlović, R. ; Tari, I. ; Csiszár, J. ; Gallé, Á. ; Poór, P. ; Galović, V. ; Trudić, B. ; Orlović, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-2b292abebbfb5e9f41f3edcb07ef75b794e42277b9320ae81ca742b7a81f441e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Ammonium</topic><topic>Antioxidants</topic><topic>Antioxidants - analysis</topic><topic>Betaine - metabolism</topic><topic>Biochemical Phenomena</topic><topic>Biomarkers</topic><topic>Biomedical and Life Sciences</topic><topic>Carbonyl compounds</topic><topic>Catalase - metabolism</topic><topic>Catechol Oxidase - metabolism</topic><topic>Cell Survival</topic><topic>Dehydration</topic><topic>Enzyme Assays</topic><topic>Enzymes - metabolism</topic><topic>Glutathione - metabolism</topic><topic>Glutathione Reductase - metabolism</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Indicator species</topic><topic>Life Sciences</topic><topic>Lipid Peroxidation</topic><topic>Nitric Oxide - metabolism</topic><topic>Osmotic Pressure</topic><topic>Oxidative Stress</topic><topic>Oxidoreductases - metabolism</topic><topic>Peroxidases - metabolism</topic><topic>Peroxidation</topic><topic>Peroxynitrous Acid - metabolism</topic><topic>Phenol - metabolism</topic><topic>Phenols</topic><topic>Plant Biochemistry</topic><topic>Plant Ecology</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - metabolism</topic><topic>Plant Leaves - chemistry</topic><topic>Plant Leaves - metabolism</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Populus - chemistry</topic><topic>Populus - enzymology</topic><topic>Populus - metabolism</topic><topic>Proline - metabolism</topic><topic>Reactive Nitrogen Species - metabolism</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Regular Paper</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Tissue Culture Techniques</topic><topic>Water - metabolism</topic><topic>Water deficit</topic><topic>Water stress</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Popović, B. M.</creatorcontrib><creatorcontrib>Štajner, D.</creatorcontrib><creatorcontrib>Ždero-Pavlović, R.</creatorcontrib><creatorcontrib>Tari, I.</creatorcontrib><creatorcontrib>Csiszár, J.</creatorcontrib><creatorcontrib>Gallé, Á.</creatorcontrib><creatorcontrib>Poór, P.</creatorcontrib><creatorcontrib>Galović, V.</creatorcontrib><creatorcontrib>Trudić, B.</creatorcontrib><creatorcontrib>Orlović, S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Environment Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of plant research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Popović, B. M.</au><au>Štajner, D.</au><au>Ždero-Pavlović, R.</au><au>Tari, I.</au><au>Csiszár, J.</au><au>Gallé, Á.</au><au>Poór, P.</au><au>Galović, V.</au><au>Trudić, B.</au><au>Orlović, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biochemical response of hybrid black poplar tissue culture (Populus × canadensis) on water stress</atitle><jtitle>Journal of plant research</jtitle><stitle>J Plant Res</stitle><addtitle>J Plant Res</addtitle><date>2017-05-01</date><risdate>2017</risdate><volume>130</volume><issue>3</issue><spage>559</spage><epage>570</epage><pages>559-570</pages><issn>0918-9440</issn><eissn>1618-0860</eissn><abstract>In this study, poplar tissue culture (hybrid black poplar, M1 genotype) was subjected to water stress influenced by polyethyleneglycol 6000 (100 and 200 mOsm PEG 6000). The aim of the research was to investigate the biochemical response of poplar tissue culture on water deficit regime. Antioxidant status was analyzed including antioxidant enzymes, superoxide-dismutase (SOD), catalase (CAT), guiacol-peroxidase (GPx), glutathione-peroxidase (GSH-Px), glutathione-reductase, reduced glutathione, total phenol content, Ferric reducing antioxidant power and DPPH radical antioxidant power. Polyphenol oxidase and phenylalanine-ammonium-lyase were determined as enzymatic markers of polyphenol metabolism. Among oxidative stress parameters lipid peroxidation, carbonyl-proteins, hydrogen-peroxide, reactive oxygen species, nitric-oxide and peroxynitrite were determined. Proline, proline-dehydrogenase and glycinebetaine were measured also as parameters of water stress. Cell viability is finally determined as a biological indicator of osmotic stress. It was found that water stress induced reactive oxygen and nitrogen species and lipid peroxidation in leaves of hybrid black poplar and reduced cell viability. Antioxidant enzymes including SOD, GPx, CAT and GSH-Px were induced but total phenol content and antioxidant capacity were reduced by PEG 6000 mediated osmotic stress. The highest biochemical response and adaptive reaction was the increase of proline and GB especially by 200 mOsm PEG. While long term molecular analysis will be necessary to fully address the poplar potentials for water stress adaptation, our results on hybrid black poplar suggest that glycine-betaine, proline and PDH enzyme might be the most important markers of poplar on water stress and that future efforts should be focused on these markers and strategies to enhance their concentration in poplar.</abstract><cop>Tokyo</cop><pub>Springer Japan</pub><pmid>28243831</pmid><doi>10.1007/s10265-017-0918-4</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0918-9440 |
| ispartof | Journal of plant research, 2017-05, Vol.130 (3), p.559-570 |
| issn | 0918-9440 1618-0860 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1872876903 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Ammonium Antioxidants Antioxidants - analysis Betaine - metabolism Biochemical Phenomena Biomarkers Biomedical and Life Sciences Carbonyl compounds Catalase - metabolism Catechol Oxidase - metabolism Cell Survival Dehydration Enzyme Assays Enzymes - metabolism Glutathione - metabolism Glutathione Reductase - metabolism Hydrogen Peroxide - metabolism Indicator species Life Sciences Lipid Peroxidation Nitric Oxide - metabolism Osmotic Pressure Oxidative Stress Oxidoreductases - metabolism Peroxidases - metabolism Peroxidation Peroxynitrous Acid - metabolism Phenol - metabolism Phenols Plant Biochemistry Plant Ecology Plant Extracts - chemistry Plant Extracts - metabolism Plant Leaves - chemistry Plant Leaves - metabolism Plant Physiology Plant Sciences Populus - chemistry Populus - enzymology Populus - metabolism Proline - metabolism Reactive Nitrogen Species - metabolism Reactive Oxygen Species - metabolism Regular Paper Superoxide Dismutase - metabolism Tissue Culture Techniques Water - metabolism Water deficit Water stress |
| title | Biochemical response of hybrid black poplar tissue culture (Populus × canadensis) on water stress |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T18%3A01%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biochemical%20response%20of%20hybrid%20black%20poplar%20tissue%20culture%20(Populus%C2%A0%C3%97%C2%A0canadensis)%20on%20water%20stress&rft.jtitle=Journal%20of%20plant%20research&rft.au=Popovi%C4%87,%20B.%20M.&rft.date=2017-05-01&rft.volume=130&rft.issue=3&rft.spage=559&rft.epage=570&rft.pages=559-570&rft.issn=0918-9440&rft.eissn=1618-0860&rft_id=info:doi/10.1007/s10265-017-0918-4&rft_dat=%3Cproquest_cross%3E1872876903%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1892125739&rft_id=info:pmid/28243831&rfr_iscdi=true |