C/EBP-α, involvement of a novel transcription factor in leptin-induced VCAM-1 production in mouse chondrocytes
•Leptin induces C/EBP-α expression.•C/EBP-α regulates VCAM-1 expression by binding to a newly identified binding site in the VCAM-1 promoter region.•Leptin induces C/EBP-α-dependent VCAM-1 expression in chondrocytes. Leptin and vascular cell adhesion molecules-1 (VCAM-1) are two important mediators...
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| Veröffentlicht in: | FEBS letters 2014-04, Vol.588 (7), p.1122-1127 |
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| container_title | FEBS letters |
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| creator | Kang, Xia Xie, Qing-Yun Zhou, Jing-Song Zhang, Bo Liao, Dong-Fa Wu, Hong-Hua Zheng, Wei Pan, Xian-Ming Quan, Yi |
| description | •Leptin induces C/EBP-α expression.•C/EBP-α regulates VCAM-1 expression by binding to a newly identified binding site in the VCAM-1 promoter region.•Leptin induces C/EBP-α-dependent VCAM-1 expression in chondrocytes.
Leptin and vascular cell adhesion molecules-1 (VCAM-1) are two important mediators in obesity-related osteoarthritis, while the molecular mechanism linking leptin to VCAM-1 production is still obscure. Here we show that leptin upregulates VCAM-1 mRNA and protein levels in a time- and dose-dependent manner. Mechanistically, leptin induces VCAM-1 promoter activity by increasing the expression of C/EBP-α and facilitating its binding to a newly identified element in the VCAM-1 gene. Gain or loss of function studies reveal a regulatory role of C/EBP-α on VCAM-1 expression. Finally, elevated plasma leptin level correlates to increased C/EBP-α and VCAM-1 production in chondrocytes from obese mice. |
| doi_str_mv | 10.1016/j.febslet.2014.02.032 |
| format | Article |
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Leptin and vascular cell adhesion molecules-1 (VCAM-1) are two important mediators in obesity-related osteoarthritis, while the molecular mechanism linking leptin to VCAM-1 production is still obscure. Here we show that leptin upregulates VCAM-1 mRNA and protein levels in a time- and dose-dependent manner. Mechanistically, leptin induces VCAM-1 promoter activity by increasing the expression of C/EBP-α and facilitating its binding to a newly identified element in the VCAM-1 gene. Gain or loss of function studies reveal a regulatory role of C/EBP-α on VCAM-1 expression. Finally, elevated plasma leptin level correlates to increased C/EBP-α and VCAM-1 production in chondrocytes from obese mice.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2014.02.032</identifier><identifier>PMID: 24582795</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; Binding Sites ; C/EBP-α ; CCAAT-enhancer-binding protein-α ; CCAAT-Enhancer-Binding Proteins - physiology ; Cells, Cultured ; Chondrocytes - metabolism ; Humans ; Leptin ; Leptin - physiology ; Male ; Mice ; Mice, Inbred C57BL ; Obesity - blood ; Osteoarthritis ; Primary Cell Culture ; Promoter Regions, Genetic ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Vascular Cell Adhesion Molecule-1 - genetics ; Vascular Cell Adhesion Molecule-1 - metabolism ; vascular cell adhesion molecules-1 ; VCAM-1</subject><ispartof>FEBS letters, 2014-04, Vol.588 (7), p.1122-1127</ispartof><rights>2014 Federation of European Biochemical Societies</rights><rights>FEBS Letters 588 (2014) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4635-582d7159b5b3dcc09b20ae87566c1c40cadc221fa481ecd8e959514afcf514723</citedby><cites>FETCH-LOGICAL-c4635-582d7159b5b3dcc09b20ae87566c1c40cadc221fa481ecd8e959514afcf514723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2014.02.032$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.febslet.2014.02.032$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,777,781,1412,1428,3537,27905,27906,45555,45556,45976,46390,46814</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24582795$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kang, Xia</creatorcontrib><creatorcontrib>Xie, Qing-Yun</creatorcontrib><creatorcontrib>Zhou, Jing-Song</creatorcontrib><creatorcontrib>Zhang, Bo</creatorcontrib><creatorcontrib>Liao, Dong-Fa</creatorcontrib><creatorcontrib>Wu, Hong-Hua</creatorcontrib><creatorcontrib>Zheng, Wei</creatorcontrib><creatorcontrib>Pan, Xian-Ming</creatorcontrib><creatorcontrib>Quan, Yi</creatorcontrib><title>C/EBP-α, involvement of a novel transcription factor in leptin-induced VCAM-1 production in mouse chondrocytes</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>•Leptin induces C/EBP-α expression.•C/EBP-α regulates VCAM-1 expression by binding to a newly identified binding site in the VCAM-1 promoter region.•Leptin induces C/EBP-α-dependent VCAM-1 expression in chondrocytes.
Leptin and vascular cell adhesion molecules-1 (VCAM-1) are two important mediators in obesity-related osteoarthritis, while the molecular mechanism linking leptin to VCAM-1 production is still obscure. Here we show that leptin upregulates VCAM-1 mRNA and protein levels in a time- and dose-dependent manner. Mechanistically, leptin induces VCAM-1 promoter activity by increasing the expression of C/EBP-α and facilitating its binding to a newly identified element in the VCAM-1 gene. Gain or loss of function studies reveal a regulatory role of C/EBP-α on VCAM-1 expression. Finally, elevated plasma leptin level correlates to increased C/EBP-α and VCAM-1 production in chondrocytes from obese mice.</description><subject>Animals</subject><subject>Binding Sites</subject><subject>C/EBP-α</subject><subject>CCAAT-enhancer-binding protein-α</subject><subject>CCAAT-Enhancer-Binding Proteins - physiology</subject><subject>Cells, Cultured</subject><subject>Chondrocytes - metabolism</subject><subject>Humans</subject><subject>Leptin</subject><subject>Leptin - physiology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Obesity - blood</subject><subject>Osteoarthritis</subject><subject>Primary Cell Culture</subject><subject>Promoter Regions, Genetic</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Vascular Cell Adhesion Molecule-1 - genetics</subject><subject>Vascular Cell Adhesion Molecule-1 - metabolism</subject><subject>vascular cell adhesion molecules-1</subject><subject>VCAM-1</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkd1u1DAQhS0EotvCI4B8yUWT2k6cnyvUrrYUqVUr8XNrOfZEeJXYi51dtI_Fi_SZOmEXbsvVeOwzx6PzEfKOs5wzXl2s8x66NMCUC8bLnImcFeIFWfCmLrKirJqXZMHwJZN1W5yQ05TWDPuGt6_JiShlI-pWLkhYXqyuHrLH3-fU-V0YdjCCn2joqaY-7GCgU9Q-meg2kwue9tpMIaKWDoA3PnPebg1Y-n15eZdxuokB-z9S1Ixhm4CaH8HbGMx-gvSGvOr1kODtsZ6Rb9err8ub7Pb-0-fl5W1myqqQGa5nay7bTnaFNYa1nWAamlpWleGmZEZbIwTvddlwMLaBVraSl7o3PZZaFGfkw8EXF_q5hTSp0SUDw6A94FIKYxKYBZf181KJgbOilLOrPEhNDClF6NUmulHHveJMzVjUWh2xqBmLYkIhFpx7f_xi241g_0395YCCm4Pglxtg_3-u6np1Jb7MjGfEvMSTFLPVx4MVYLw7B1El48AjIxfBTMoG98y2T3iutrM</recordid><startdate>20140402</startdate><enddate>20140402</enddate><creator>Kang, Xia</creator><creator>Xie, Qing-Yun</creator><creator>Zhou, Jing-Song</creator><creator>Zhang, Bo</creator><creator>Liao, Dong-Fa</creator><creator>Wu, Hong-Hua</creator><creator>Zheng, Wei</creator><creator>Pan, Xian-Ming</creator><creator>Quan, Yi</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QP</scope><scope>7TM</scope></search><sort><creationdate>20140402</creationdate><title>C/EBP-α, involvement of a novel transcription factor in leptin-induced VCAM-1 production in mouse chondrocytes</title><author>Kang, Xia ; Xie, Qing-Yun ; Zhou, Jing-Song ; Zhang, Bo ; Liao, Dong-Fa ; Wu, Hong-Hua ; Zheng, Wei ; Pan, Xian-Ming ; Quan, Yi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4635-582d7159b5b3dcc09b20ae87566c1c40cadc221fa481ecd8e959514afcf514723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Binding Sites</topic><topic>C/EBP-α</topic><topic>CCAAT-enhancer-binding protein-α</topic><topic>CCAAT-Enhancer-Binding Proteins - physiology</topic><topic>Cells, Cultured</topic><topic>Chondrocytes - metabolism</topic><topic>Humans</topic><topic>Leptin</topic><topic>Leptin - physiology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Obesity - blood</topic><topic>Osteoarthritis</topic><topic>Primary Cell Culture</topic><topic>Promoter Regions, Genetic</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Vascular Cell Adhesion Molecule-1 - genetics</topic><topic>Vascular Cell Adhesion Molecule-1 - metabolism</topic><topic>vascular cell adhesion molecules-1</topic><topic>VCAM-1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kang, Xia</creatorcontrib><creatorcontrib>Xie, Qing-Yun</creatorcontrib><creatorcontrib>Zhou, Jing-Song</creatorcontrib><creatorcontrib>Zhang, Bo</creatorcontrib><creatorcontrib>Liao, Dong-Fa</creatorcontrib><creatorcontrib>Wu, Hong-Hua</creatorcontrib><creatorcontrib>Zheng, Wei</creatorcontrib><creatorcontrib>Pan, Xian-Ming</creatorcontrib><creatorcontrib>Quan, Yi</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nucleic Acids Abstracts</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kang, Xia</au><au>Xie, Qing-Yun</au><au>Zhou, Jing-Song</au><au>Zhang, Bo</au><au>Liao, Dong-Fa</au><au>Wu, Hong-Hua</au><au>Zheng, Wei</au><au>Pan, Xian-Ming</au><au>Quan, Yi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>C/EBP-α, involvement of a novel transcription factor in leptin-induced VCAM-1 production in mouse chondrocytes</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2014-04-02</date><risdate>2014</risdate><volume>588</volume><issue>7</issue><spage>1122</spage><epage>1127</epage><pages>1122-1127</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>•Leptin induces C/EBP-α expression.•C/EBP-α regulates VCAM-1 expression by binding to a newly identified binding site in the VCAM-1 promoter region.•Leptin induces C/EBP-α-dependent VCAM-1 expression in chondrocytes.
Leptin and vascular cell adhesion molecules-1 (VCAM-1) are two important mediators in obesity-related osteoarthritis, while the molecular mechanism linking leptin to VCAM-1 production is still obscure. Here we show that leptin upregulates VCAM-1 mRNA and protein levels in a time- and dose-dependent manner. Mechanistically, leptin induces VCAM-1 promoter activity by increasing the expression of C/EBP-α and facilitating its binding to a newly identified element in the VCAM-1 gene. Gain or loss of function studies reveal a regulatory role of C/EBP-α on VCAM-1 expression. Finally, elevated plasma leptin level correlates to increased C/EBP-α and VCAM-1 production in chondrocytes from obese mice.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>24582795</pmid><doi>10.1016/j.febslet.2014.02.032</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; Alma/SFX Local Collection |
| subjects | Animals Binding Sites C/EBP-α CCAAT-enhancer-binding protein-α CCAAT-Enhancer-Binding Proteins - physiology Cells, Cultured Chondrocytes - metabolism Humans Leptin Leptin - physiology Male Mice Mice, Inbred C57BL Obesity - blood Osteoarthritis Primary Cell Culture Promoter Regions, Genetic RNA, Messenger - genetics RNA, Messenger - metabolism Vascular Cell Adhesion Molecule-1 - genetics Vascular Cell Adhesion Molecule-1 - metabolism vascular cell adhesion molecules-1 VCAM-1 |
| title | C/EBP-α, involvement of a novel transcription factor in leptin-induced VCAM-1 production in mouse chondrocytes |
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