Generation of Induced Pluripotent Stem Cells From Patients With Duchenne Muscular Dystrophy and Their Induction to Cardiomyocytes
Duchenne muscular dystrophy (DMD) is caused by mutations in the DMD gene which encodes dystrophin protein. Dystrophin defect affects cardiac muscle as well as skeletal muscle. Cardiac dysfunction is observed in all patients with DMD over 18 years of age, but there is no curative treatment for DMD ca...
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| Veröffentlicht in: | International Heart Journal 2016, Vol.57(1), pp.112-117 |
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| creator | Hashimoto, Akihito Naito, Atsuhiko T. Lee, Jong-Kook Kitazume-Taneike, Rika Ito, Masamichi Yamaguchi, Toshihiro Nakata, Ryo Sumida, Tomokazu Okada, Katsuki Nakagawa, Akito Higo, Tomoaki Kuramoto, Yuki Sakai, Taku Tominaga, Koji Okinaga, Takeshi Kogaki, Shigetoyo Ozono, Keiichi Miyagawa, Shigeru Sawa, Yoshiki Sakata, Yasushi Morita, Hiroyuki Umezawa, Akihiro Komuro, Issei |
| description | Duchenne muscular dystrophy (DMD) is caused by mutations in the DMD gene which encodes dystrophin protein. Dystrophin defect affects cardiac muscle as well as skeletal muscle. Cardiac dysfunction is observed in all patients with DMD over 18 years of age, but there is no curative treatment for DMD cardiomyopathy. To establish novel experimental platforms which reproduce the cardiac phenotype of DMD patients, here we established iPS cell lines from T lymphocytes donated from two DMD patients, with a protocol using Sendai virus vectors. We successfully conducted the differentiation of the DMD patient-specific iPS cells into beating cardiomyocytes. DMD patient-specific iPS cells and iPS cell-derived cardiomyocytes would be a useful in vitro experimental system with which to investigate DMD cardiomyopathy. |
| doi_str_mv | 10.1536/ihj.15-376 |
| format | Article |
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Dystrophin defect affects cardiac muscle as well as skeletal muscle. Cardiac dysfunction is observed in all patients with DMD over 18 years of age, but there is no curative treatment for DMD cardiomyopathy. To establish novel experimental platforms which reproduce the cardiac phenotype of DMD patients, here we established iPS cell lines from T lymphocytes donated from two DMD patients, with a protocol using Sendai virus vectors. We successfully conducted the differentiation of the DMD patient-specific iPS cells into beating cardiomyocytes. DMD patient-specific iPS cells and iPS cell-derived cardiomyocytes would be a useful in vitro experimental system with which to investigate DMD cardiomyopathy.</description><identifier>ISSN: 1349-2365</identifier><identifier>EISSN: 1349-3299</identifier><identifier>DOI: 10.1536/ihj.15-376</identifier><identifier>PMID: 26673445</identifier><language>eng</language><publisher>Japan: International Heart Journal Association</publisher><subject>Adolescent ; Adult ; Cardiomyopathy ; Cell Differentiation ; Cells, Cultured ; Human ; Humans ; Induced Pluripotent Stem Cells - cytology ; Induced Pluripotent Stem Cells - physiology ; Muscular Dystrophy, Duchenne - genetics ; Muscular Dystrophy, Duchenne - metabolism ; Muscular Dystrophy, Duchenne - pathology ; Myocytes, Cardiac - cytology ; Myocytes, Cardiac - metabolism ; Real-Time Polymerase Chain Reaction ; RNA - genetics</subject><ispartof>International Heart Journal, 2016, Vol.57(1), pp.112-117</ispartof><rights>2016 by the International Heart Journal Association</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-dac3448ea049fa83df57f6c981d24b2f95e64e9b87764c5a29508571f2eca86c3</citedby><cites>FETCH-LOGICAL-c503t-dac3448ea049fa83df57f6c981d24b2f95e64e9b87764c5a29508571f2eca86c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26673445$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hashimoto, Akihito</creatorcontrib><creatorcontrib>Naito, Atsuhiko T.</creatorcontrib><creatorcontrib>Lee, Jong-Kook</creatorcontrib><creatorcontrib>Kitazume-Taneike, Rika</creatorcontrib><creatorcontrib>Ito, Masamichi</creatorcontrib><creatorcontrib>Yamaguchi, Toshihiro</creatorcontrib><creatorcontrib>Nakata, Ryo</creatorcontrib><creatorcontrib>Sumida, Tomokazu</creatorcontrib><creatorcontrib>Okada, Katsuki</creatorcontrib><creatorcontrib>Nakagawa, Akito</creatorcontrib><creatorcontrib>Higo, Tomoaki</creatorcontrib><creatorcontrib>Kuramoto, Yuki</creatorcontrib><creatorcontrib>Sakai, Taku</creatorcontrib><creatorcontrib>Tominaga, Koji</creatorcontrib><creatorcontrib>Okinaga, Takeshi</creatorcontrib><creatorcontrib>Kogaki, Shigetoyo</creatorcontrib><creatorcontrib>Ozono, Keiichi</creatorcontrib><creatorcontrib>Miyagawa, Shigeru</creatorcontrib><creatorcontrib>Sawa, Yoshiki</creatorcontrib><creatorcontrib>Sakata, Yasushi</creatorcontrib><creatorcontrib>Morita, Hiroyuki</creatorcontrib><creatorcontrib>Umezawa, Akihiro</creatorcontrib><creatorcontrib>Komuro, Issei</creatorcontrib><title>Generation of Induced Pluripotent Stem Cells From Patients With Duchenne Muscular Dystrophy and Their Induction to Cardiomyocytes</title><title>International Heart Journal</title><addtitle>Int. Heart J.</addtitle><description>Duchenne muscular dystrophy (DMD) is caused by mutations in the DMD gene which encodes dystrophin protein. Dystrophin defect affects cardiac muscle as well as skeletal muscle. Cardiac dysfunction is observed in all patients with DMD over 18 years of age, but there is no curative treatment for DMD cardiomyopathy. To establish novel experimental platforms which reproduce the cardiac phenotype of DMD patients, here we established iPS cell lines from T lymphocytes donated from two DMD patients, with a protocol using Sendai virus vectors. We successfully conducted the differentiation of the DMD patient-specific iPS cells into beating cardiomyocytes. DMD patient-specific iPS cells and iPS cell-derived cardiomyocytes would be a useful in vitro experimental system with which to investigate DMD cardiomyopathy.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Cardiomyopathy</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Human</subject><subject>Humans</subject><subject>Induced Pluripotent Stem Cells - cytology</subject><subject>Induced Pluripotent Stem Cells - physiology</subject><subject>Muscular Dystrophy, Duchenne - genetics</subject><subject>Muscular Dystrophy, Duchenne - metabolism</subject><subject>Muscular Dystrophy, Duchenne - pathology</subject><subject>Myocytes, Cardiac - cytology</subject><subject>Myocytes, Cardiac - metabolism</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>RNA - genetics</subject><issn>1349-2365</issn><issn>1349-3299</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM1u1DAUha2qqC2FTR8AeVlVCsTxb3ag6Q-ViqhEEUvL49w0HiX2YDuLLHlzzMwwG_vI_vRd-yB0ReqPhFPxyQ2bEioqxQm6IJS1FW3a9vSQGyr4OXqb0qauGeG1PEPnjRCSMsYv0J8H8BBNdsHj0ONH380WOvw8ztFtQwaf8Y8ME17BOCZ8H8OEnwtdzhP-5fKAb2c7gPeAv83JzqOJ-HZJOYbtsGDjO_wygIt7725IDnhlYufCtAS7ZEjv0JvejAneH_ZL9PP-7mX1tXr6_vC4-vJUWV7TXHXGlhcrMDVre6No13PZC9sq0jVs3fQtB8GgXSspBbPcNC2vFZekb8AaJSy9RNd77zaG3zOkrCeXbPmW8RDmpImSjSKMMFXQmz1qY0gpQq-30U0mLprU-l_lulRegi6VF_jDwTuvJ-iO6P-OC_B5D2xSNq9wBEzMzo6wc3GpyW7ZOY9XdjBRg6d_ATLVlcE</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Hashimoto, Akihito</creator><creator>Naito, Atsuhiko T.</creator><creator>Lee, Jong-Kook</creator><creator>Kitazume-Taneike, Rika</creator><creator>Ito, Masamichi</creator><creator>Yamaguchi, Toshihiro</creator><creator>Nakata, Ryo</creator><creator>Sumida, Tomokazu</creator><creator>Okada, Katsuki</creator><creator>Nakagawa, Akito</creator><creator>Higo, Tomoaki</creator><creator>Kuramoto, Yuki</creator><creator>Sakai, Taku</creator><creator>Tominaga, Koji</creator><creator>Okinaga, Takeshi</creator><creator>Kogaki, Shigetoyo</creator><creator>Ozono, Keiichi</creator><creator>Miyagawa, Shigeru</creator><creator>Sawa, Yoshiki</creator><creator>Sakata, Yasushi</creator><creator>Morita, Hiroyuki</creator><creator>Umezawa, Akihiro</creator><creator>Komuro, Issei</creator><general>International Heart Journal Association</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20160101</creationdate><title>Generation of Induced Pluripotent Stem Cells From Patients With Duchenne Muscular Dystrophy and Their Induction to Cardiomyocytes</title><author>Hashimoto, Akihito ; Naito, Atsuhiko T. ; Lee, Jong-Kook ; Kitazume-Taneike, Rika ; Ito, Masamichi ; Yamaguchi, Toshihiro ; Nakata, Ryo ; Sumida, Tomokazu ; Okada, Katsuki ; Nakagawa, Akito ; Higo, Tomoaki ; Kuramoto, Yuki ; Sakai, Taku ; Tominaga, Koji ; Okinaga, Takeshi ; Kogaki, Shigetoyo ; Ozono, Keiichi ; Miyagawa, Shigeru ; Sawa, Yoshiki ; Sakata, Yasushi ; Morita, Hiroyuki ; Umezawa, Akihiro ; Komuro, Issei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-dac3448ea049fa83df57f6c981d24b2f95e64e9b87764c5a29508571f2eca86c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Cardiomyopathy</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Human</topic><topic>Humans</topic><topic>Induced Pluripotent Stem Cells - cytology</topic><topic>Induced Pluripotent Stem Cells - physiology</topic><topic>Muscular Dystrophy, Duchenne - genetics</topic><topic>Muscular Dystrophy, Duchenne - metabolism</topic><topic>Muscular Dystrophy, Duchenne - pathology</topic><topic>Myocytes, Cardiac - cytology</topic><topic>Myocytes, Cardiac - metabolism</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>RNA - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hashimoto, Akihito</creatorcontrib><creatorcontrib>Naito, Atsuhiko T.</creatorcontrib><creatorcontrib>Lee, Jong-Kook</creatorcontrib><creatorcontrib>Kitazume-Taneike, Rika</creatorcontrib><creatorcontrib>Ito, Masamichi</creatorcontrib><creatorcontrib>Yamaguchi, Toshihiro</creatorcontrib><creatorcontrib>Nakata, Ryo</creatorcontrib><creatorcontrib>Sumida, Tomokazu</creatorcontrib><creatorcontrib>Okada, Katsuki</creatorcontrib><creatorcontrib>Nakagawa, Akito</creatorcontrib><creatorcontrib>Higo, Tomoaki</creatorcontrib><creatorcontrib>Kuramoto, Yuki</creatorcontrib><creatorcontrib>Sakai, Taku</creatorcontrib><creatorcontrib>Tominaga, Koji</creatorcontrib><creatorcontrib>Okinaga, Takeshi</creatorcontrib><creatorcontrib>Kogaki, Shigetoyo</creatorcontrib><creatorcontrib>Ozono, Keiichi</creatorcontrib><creatorcontrib>Miyagawa, Shigeru</creatorcontrib><creatorcontrib>Sawa, Yoshiki</creatorcontrib><creatorcontrib>Sakata, Yasushi</creatorcontrib><creatorcontrib>Morita, Hiroyuki</creatorcontrib><creatorcontrib>Umezawa, Akihiro</creatorcontrib><creatorcontrib>Komuro, Issei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International Heart Journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hashimoto, Akihito</au><au>Naito, Atsuhiko T.</au><au>Lee, Jong-Kook</au><au>Kitazume-Taneike, Rika</au><au>Ito, Masamichi</au><au>Yamaguchi, Toshihiro</au><au>Nakata, Ryo</au><au>Sumida, Tomokazu</au><au>Okada, Katsuki</au><au>Nakagawa, Akito</au><au>Higo, Tomoaki</au><au>Kuramoto, Yuki</au><au>Sakai, Taku</au><au>Tominaga, Koji</au><au>Okinaga, Takeshi</au><au>Kogaki, Shigetoyo</au><au>Ozono, Keiichi</au><au>Miyagawa, Shigeru</au><au>Sawa, Yoshiki</au><au>Sakata, Yasushi</au><au>Morita, Hiroyuki</au><au>Umezawa, Akihiro</au><au>Komuro, Issei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Generation of Induced Pluripotent Stem Cells From Patients With Duchenne Muscular Dystrophy and Their Induction to Cardiomyocytes</atitle><jtitle>International Heart Journal</jtitle><addtitle>Int. Heart J.</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>57</volume><issue>1</issue><spage>112</spage><epage>117</epage><pages>112-117</pages><issn>1349-2365</issn><eissn>1349-3299</eissn><abstract>Duchenne muscular dystrophy (DMD) is caused by mutations in the DMD gene which encodes dystrophin protein. Dystrophin defect affects cardiac muscle as well as skeletal muscle. Cardiac dysfunction is observed in all patients with DMD over 18 years of age, but there is no curative treatment for DMD cardiomyopathy. To establish novel experimental platforms which reproduce the cardiac phenotype of DMD patients, here we established iPS cell lines from T lymphocytes donated from two DMD patients, with a protocol using Sendai virus vectors. We successfully conducted the differentiation of the DMD patient-specific iPS cells into beating cardiomyocytes. DMD patient-specific iPS cells and iPS cell-derived cardiomyocytes would be a useful in vitro experimental system with which to investigate DMD cardiomyopathy.</abstract><cop>Japan</cop><pub>International Heart Journal Association</pub><pmid>26673445</pmid><doi>10.1536/ihj.15-376</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Adult Cardiomyopathy Cell Differentiation Cells, Cultured Human Humans Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - physiology Muscular Dystrophy, Duchenne - genetics Muscular Dystrophy, Duchenne - metabolism Muscular Dystrophy, Duchenne - pathology Myocytes, Cardiac - cytology Myocytes, Cardiac - metabolism Real-Time Polymerase Chain Reaction RNA - genetics |
| title | Generation of Induced Pluripotent Stem Cells From Patients With Duchenne Muscular Dystrophy and Their Induction to Cardiomyocytes |
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