Identification and evaluation of reference genes for accurate gene expression normalization of fresh and frozen-thawed spermatozoa of water buffalo (Bubalus bubalis)

The quantitative real time PCR (qRT-PCR) has become an important tool for gene-expression analysis for a selected number of genes in life science. Although large dynamic range, sensitivity and reproducibility of qRT-PCR is good, the reliability majorly depend on the selection of proper reference gen...

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Veröffentlicht in:	Theriogenology 2017-04, Vol.92, p.6-13
Hauptverfasser:	Ashish, Shende, Bhure, S.K., Harikrishna, Pillai, Ramteke, S.S., Muhammed Kutty, V.H., Shruthi, N., Ravi Kumar, G.V.P.P.S., Manish, Mahawar, Ghosh, S.K., Mihir, Sarkar
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Buffalo Buffaloes - physiology Cryopreservation Freezing Gene Expression Profiling - standards Gene Expression Profiling - veterinary Gene Expression Regulation - physiology Male Normalization qRT-PCR Reference gene Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction - veterinary Semen Preservation - veterinary Sperm Spermatozoa - metabolism
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