Detection of histidine-rich glycoprotein and fibrinogen with nickel-enzyme conjugates: Purification of rabbit HRG

Nickel-bound alkaline phosphatase and peroxidase enzymes were used to investigate nickel binding to plasma proteins. Rabbit plasma dilutions to 25,000 were positive by ELISA, while Western blot analysis showed a prominent reaction with histidine-rich glycoprotein (HRG)1 and lower reaction with fibri...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Analytical biochemistry 2017-05, Vol.525, p.67-72
Hauptverfasser:	Colwell, Mathia, Ahmed, Najma, Butkowski, Ralph
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline Phosphatase - chemistry Alkaline Phosphatase - metabolism Animals Blotting, Western Cattle Chickens Chromatography, Ion Exchange Enzyme-Linked Immunosorbent Assay Fibrinogen Fibrinogen - analysis Fibrinogen - chemistry Fibrinogen - metabolism Guinea Pigs Histidine-rich glycoprotein Humans Nickel - chemistry Nickel - metabolism Nickel-enzyme detection Peroxidase - chemistry Peroxidase - metabolism Plasma proteins Protein Binding Proteins - analysis Proteins - isolation & purification Proteins - metabolism Rabbits
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!