Effects of Root Extracts of Eurycoma longifolia Jack on Corpus Cavernosum of Rat
Objective: This study was conducted to investigate the mechanisms of action of Eurycoma longifolia in rat corpus cavernosum. Materials and Methods: Tincture of the roots was concentrated to dryness by evaporating the ethanol in vacuo. This ethanolic extract was partitioned into 5 fractions sequentia...
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| Veröffentlicht in: | Medical principles and practice 2017-01, Vol.26 (3), p.258-265 |
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| creator | Tee, Bae Huey Hoe, See Ziau Cheah, Swee Hung Lam, Sau Kuen |
| description | Objective: This study was conducted to investigate the mechanisms of action of Eurycoma longifolia in rat corpus cavernosum. Materials and Methods: Tincture of the roots was concentrated to dryness by evaporating the ethanol in vacuo. This ethanolic extract was partitioned into 5 fractions sequentially with hexane, dichloromethane (DCM), ethyl acetate, butanol, and water. The corpus cavernosum relaxant activity of each fraction was investigated. The DCM fraction which showed the highest potency in relaxing phenylephrine-precontracted corpora cavernosa was purified by column chromatography. The effects of the most potent DCM subfraction in relaxing phenylephrine-precontracted corpora cavernosa, DCM-I, on angiotensin I- or angiotensin II-induced contractions in corpora cavernosa were investigated. The effects of DCM-I pretreatment on the responses of phenylephrine-precontracted corpora cavernosa to angiotensin II or bradykinin were also studied. An in vitro assay was conducted to evaluate the effect of DCM-I on angiotensin-converting enzyme activity. Results: Fraction DCM-I decreased the maximal contractions (100%) evoked by angiotensin I and angiotensin II to 30 ± 14% and 26 ± 16% (p < 0.001), respectively. In phenylephrine-precontracted corpora cavernosa, DCM-I pretreatment caused angiotensin II to induce 82 ± 27% relaxation of maximal contraction (p < 0.01) and enhanced (p < 0.001) bradykinin-induced relaxations from 47 ± 8% to 100 ± 5%. In vitro, DCM-I was able to reduce (p < 0.001) the maximal angiotensin-converting enzyme activity to 78 ± 0.24%. Conclusion: Fraction DCM-I was able to antagonize angiotensin II-induced contraction to cause corpus cavernosum relaxation via inhibition of angiotensin II type 1 receptor and enhance bradykinin-induced relaxation through inhibition of angiotensin-converting enzyme. |
| doi_str_mv | 10.1159/000464363 |
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Materials and Methods: Tincture of the roots was concentrated to dryness by evaporating the ethanol in vacuo. This ethanolic extract was partitioned into 5 fractions sequentially with hexane, dichloromethane (DCM), ethyl acetate, butanol, and water. The corpus cavernosum relaxant activity of each fraction was investigated. The DCM fraction which showed the highest potency in relaxing phenylephrine-precontracted corpora cavernosa was purified by column chromatography. The effects of the most potent DCM subfraction in relaxing phenylephrine-precontracted corpora cavernosa, DCM-I, on angiotensin I- or angiotensin II-induced contractions in corpora cavernosa were investigated. The effects of DCM-I pretreatment on the responses of phenylephrine-precontracted corpora cavernosa to angiotensin II or bradykinin were also studied. An in vitro assay was conducted to evaluate the effect of DCM-I on angiotensin-converting enzyme activity. Results: Fraction DCM-I decreased the maximal contractions (100%) evoked by angiotensin I and angiotensin II to 30 ± 14% and 26 ± 16% (p < 0.001), respectively. In phenylephrine-precontracted corpora cavernosa, DCM-I pretreatment caused angiotensin II to induce 82 ± 27% relaxation of maximal contraction (p < 0.01) and enhanced (p < 0.001) bradykinin-induced relaxations from 47 ± 8% to 100 ± 5%. In vitro, DCM-I was able to reduce (p < 0.001) the maximal angiotensin-converting enzyme activity to 78 ± 0.24%. Conclusion: Fraction DCM-I was able to antagonize angiotensin II-induced contraction to cause corpus cavernosum relaxation via inhibition of angiotensin II type 1 receptor and enhance bradykinin-induced relaxation through inhibition of angiotensin-converting enzyme.</description><identifier>ISSN: 1011-7571</identifier><identifier>EISSN: 1423-0151</identifier><identifier>DOI: 10.1159/000464363</identifier><identifier>PMID: 28226311</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>Angiotensin I - pharmacology ; Angiotensin II - pharmacology ; Animals ; Enzymes ; Erectile dysfunction ; Ethanol ; Eurycoma ; Hearing loss ; In Vitro Techniques ; Laboratory animals ; Male ; Muscle Contraction - drug effects ; Muscle Relaxation - drug effects ; Original Paper ; Penis - drug effects ; Peptidyl-Dipeptidase A - drug effects ; Phenylephrine - pharmacology ; Plant Extracts - pharmacology ; Plant Roots ; Rats ; Rats, Sprague-Dawley ; Rodents ; Smooth muscle ; Urology</subject><ispartof>Medical principles and practice, 2017-01, Vol.26 (3), p.258-265</ispartof><rights>2017 S. Karger AG, Basel</rights><rights>2017 S. Karger AG, Basel.</rights><rights>Copyright © 2017 by S. Karger AG, Basel 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-bbe5823864858ff370abafbd1d47cb8486184dbb18b089d178ffa6e7a85da483</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5588405/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5588405/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27612,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28226311$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tee, Bae Huey</creatorcontrib><creatorcontrib>Hoe, See Ziau</creatorcontrib><creatorcontrib>Cheah, Swee Hung</creatorcontrib><creatorcontrib>Lam, Sau Kuen</creatorcontrib><title>Effects of Root Extracts of Eurycoma longifolia Jack on Corpus Cavernosum of Rat</title><title>Medical principles and practice</title><addtitle>Med Princ Pract</addtitle><description>Objective: This study was conducted to investigate the mechanisms of action of Eurycoma longifolia in rat corpus cavernosum. Materials and Methods: Tincture of the roots was concentrated to dryness by evaporating the ethanol in vacuo. This ethanolic extract was partitioned into 5 fractions sequentially with hexane, dichloromethane (DCM), ethyl acetate, butanol, and water. The corpus cavernosum relaxant activity of each fraction was investigated. The DCM fraction which showed the highest potency in relaxing phenylephrine-precontracted corpora cavernosa was purified by column chromatography. The effects of the most potent DCM subfraction in relaxing phenylephrine-precontracted corpora cavernosa, DCM-I, on angiotensin I- or angiotensin II-induced contractions in corpora cavernosa were investigated. The effects of DCM-I pretreatment on the responses of phenylephrine-precontracted corpora cavernosa to angiotensin II or bradykinin were also studied. An in vitro assay was conducted to evaluate the effect of DCM-I on angiotensin-converting enzyme activity. Results: Fraction DCM-I decreased the maximal contractions (100%) evoked by angiotensin I and angiotensin II to 30 ± 14% and 26 ± 16% (p < 0.001), respectively. In phenylephrine-precontracted corpora cavernosa, DCM-I pretreatment caused angiotensin II to induce 82 ± 27% relaxation of maximal contraction (p < 0.01) and enhanced (p < 0.001) bradykinin-induced relaxations from 47 ± 8% to 100 ± 5%. In vitro, DCM-I was able to reduce (p < 0.001) the maximal angiotensin-converting enzyme activity to 78 ± 0.24%. Conclusion: Fraction DCM-I was able to antagonize angiotensin II-induced contraction to cause corpus cavernosum relaxation via inhibition of angiotensin II type 1 receptor and enhance bradykinin-induced relaxation through inhibition of angiotensin-converting enzyme.</description><subject>Angiotensin I - pharmacology</subject><subject>Angiotensin II - pharmacology</subject><subject>Animals</subject><subject>Enzymes</subject><subject>Erectile dysfunction</subject><subject>Ethanol</subject><subject>Eurycoma</subject><subject>Hearing loss</subject><subject>In Vitro Techniques</subject><subject>Laboratory animals</subject><subject>Male</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle Relaxation - drug effects</subject><subject>Original Paper</subject><subject>Penis - drug effects</subject><subject>Peptidyl-Dipeptidase A - drug effects</subject><subject>Phenylephrine - pharmacology</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Roots</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Rodents</subject><subject>Smooth muscle</subject><subject>Urology</subject><issn>1011-7571</issn><issn>1423-0151</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>M--</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpd0UtLxDAUBeAgiu-Fe5GCG11Uc5ukvbMRZBhfCIq4D0mbjNW2GZNW9N8bnXFQVwnJl8MJl5A9oCcAYnRKKeU5ZzlbIZvAM5ZSELAa9xQgLUQBG2QrhOfIkDG6TjYyzLKcAWyS-4m1puxD4mzy4FyfTN57rxYHk8F_lK5VSeO6aW1dU6vkRpUvieuSsfOzISRj9WZ858LQfieofoesWdUEs7tYt8njxeRxfJXe3l1ej89v05JnvE-1NgIzhjlHgdaygiqtrK6g4kWpkWMOyCutATXFUQVFRCo3hUJRKY5sm5zNY2eDbk1Vmi7WbuTM163yH9KpWv696eonOXVvUghETkUMOFoEePc6mNDLtg6laRrVGTcECViAEJRDFunhP_rsBt_F30kYjShmgAhRHc9V6V0I3thlGaDya0xyOaZoD363X8qfuUSwPwcvyk-NX4LF-08wCZW_</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>Tee, Bae Huey</creator><creator>Hoe, See Ziau</creator><creator>Cheah, Swee Hung</creator><creator>Lam, Sau Kuen</creator><general>S. Karger AG</general><scope>M--</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170101</creationdate><title>Effects of Root Extracts of Eurycoma longifolia Jack on Corpus Cavernosum of Rat</title><author>Tee, Bae Huey ; Hoe, See Ziau ; Cheah, Swee Hung ; Lam, Sau Kuen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-bbe5823864858ff370abafbd1d47cb8486184dbb18b089d178ffa6e7a85da483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Angiotensin I - pharmacology</topic><topic>Angiotensin II - pharmacology</topic><topic>Animals</topic><topic>Enzymes</topic><topic>Erectile dysfunction</topic><topic>Ethanol</topic><topic>Eurycoma</topic><topic>Hearing loss</topic><topic>In Vitro Techniques</topic><topic>Laboratory animals</topic><topic>Male</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle Relaxation - drug effects</topic><topic>Original Paper</topic><topic>Penis - drug effects</topic><topic>Peptidyl-Dipeptidase A - drug effects</topic><topic>Phenylephrine - pharmacology</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Roots</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Rodents</topic><topic>Smooth muscle</topic><topic>Urology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tee, Bae Huey</creatorcontrib><creatorcontrib>Hoe, See Ziau</creatorcontrib><creatorcontrib>Cheah, Swee Hung</creatorcontrib><creatorcontrib>Lam, Sau Kuen</creatorcontrib><collection>Karger Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Medical principles and practice</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tee, Bae Huey</au><au>Hoe, See Ziau</au><au>Cheah, Swee Hung</au><au>Lam, Sau Kuen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Root Extracts of Eurycoma longifolia Jack on Corpus Cavernosum of Rat</atitle><jtitle>Medical principles and practice</jtitle><addtitle>Med Princ Pract</addtitle><date>2017-01-01</date><risdate>2017</risdate><volume>26</volume><issue>3</issue><spage>258</spage><epage>265</epage><pages>258-265</pages><issn>1011-7571</issn><eissn>1423-0151</eissn><abstract>Objective: This study was conducted to investigate the mechanisms of action of Eurycoma longifolia in rat corpus cavernosum. Materials and Methods: Tincture of the roots was concentrated to dryness by evaporating the ethanol in vacuo. This ethanolic extract was partitioned into 5 fractions sequentially with hexane, dichloromethane (DCM), ethyl acetate, butanol, and water. The corpus cavernosum relaxant activity of each fraction was investigated. The DCM fraction which showed the highest potency in relaxing phenylephrine-precontracted corpora cavernosa was purified by column chromatography. The effects of the most potent DCM subfraction in relaxing phenylephrine-precontracted corpora cavernosa, DCM-I, on angiotensin I- or angiotensin II-induced contractions in corpora cavernosa were investigated. The effects of DCM-I pretreatment on the responses of phenylephrine-precontracted corpora cavernosa to angiotensin II or bradykinin were also studied. An in vitro assay was conducted to evaluate the effect of DCM-I on angiotensin-converting enzyme activity. Results: Fraction DCM-I decreased the maximal contractions (100%) evoked by angiotensin I and angiotensin II to 30 ± 14% and 26 ± 16% (p < 0.001), respectively. In phenylephrine-precontracted corpora cavernosa, DCM-I pretreatment caused angiotensin II to induce 82 ± 27% relaxation of maximal contraction (p < 0.01) and enhanced (p < 0.001) bradykinin-induced relaxations from 47 ± 8% to 100 ± 5%. In vitro, DCM-I was able to reduce (p < 0.001) the maximal angiotensin-converting enzyme activity to 78 ± 0.24%. Conclusion: Fraction DCM-I was able to antagonize angiotensin II-induced contraction to cause corpus cavernosum relaxation via inhibition of angiotensin II type 1 receptor and enhance bradykinin-induced relaxation through inhibition of angiotensin-converting enzyme.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>28226311</pmid><doi>10.1159/000464363</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Karger Open Access |
| subjects | Angiotensin I - pharmacology Angiotensin II - pharmacology Animals Enzymes Erectile dysfunction Ethanol Eurycoma Hearing loss In Vitro Techniques Laboratory animals Male Muscle Contraction - drug effects Muscle Relaxation - drug effects Original Paper Penis - drug effects Peptidyl-Dipeptidase A - drug effects Phenylephrine - pharmacology Plant Extracts - pharmacology Plant Roots Rats Rats, Sprague-Dawley Rodents Smooth muscle Urology |
| title | Effects of Root Extracts of Eurycoma longifolia Jack on Corpus Cavernosum of Rat |
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