Subunit Exchange and the Role of Dimer Flexibility in DNA Binding by the Fis Protein

Fis is an abundant bacterial DNA binding protein that functions in many different reactions. We show here that Fis subunits rapidly exchange between dimers in solution by disulfide cross-linking mixtures of Fis mutants with different electrophoretic mobilities and by monitoring energy transfer betwe...

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Veröffentlicht in:Biochemistry (Easton) 2002-05, Vol.41 (18), p.5788-5798
Hauptverfasser: Merickel, Stacy K, Sanders, Erin R, Vázquez-Ibar, José Luis, Johnson, Reid C
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Sprache:eng
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