Hepatitis B virus genotypes among chronic hepatitis B patients reporting at Korle-Bu teaching hospital, Accra, Ghana
Knowledge of hepatitis B virus (HBV) genotype is an important predictive variable which might have an impact in management and treatment of patients with chronic hepatitis B infection. In Ghana very little information is available on hepatitis B genotypes. This study was conducted to determine the d...
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| Veröffentlicht in: | The Pan African medical journal 2016, Vol.25 (Suppl 1), p.5-5 |
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| creator | Dongdem, Anthony Zunuo Dzodzomenyo, Mawuli Asmah, Richard Harry Nyarko, Kofi Mensah Nortey, Priscillia Agyei, Adwoa Adjei, David Nana Kenu, Ernest Adjei, Andrew Anthony |
| description | Knowledge of hepatitis B virus (HBV) genotype is an important predictive variable which might have an impact in management and treatment of patients with chronic hepatitis B infection. In Ghana very little information is available on hepatitis B genotypes. This study was conducted to determine the distribution of HBV genotypes circulating among chronic hepatitis B patients reporting at the Korle-Bu Teaching Hospital (KBTH), Accra, Ghana.
Blood samples (10 ml) were collected from 250 consenting patients. DNA was extracted and amplified using polymerase chain reaction technique. Restriction fragment length polymorphism (RFLP) was used for the detection of genotypes.
Out of the 250 chronic hepatitis B patients who were HBsAg positive, 91 (36.4%) were males aged 29.8 ± 9.1 and 159 (63.6%) females aged 33± 12.1 years. HBV DNA was detected in 111 (44.4%) but only 58 (52%) of these were typeable. These were classified as genotype A, 8 (7.2%); genotype D, 3 (2.7%) and genotype E, 47 (42.3%). Our results did not show any association between the infecting genotype and age (X
= 0.923; p-value=0.623) or gender (X
= 0.283, p= 0.579).
Consistent with similar studies worldwide, the results suggest that genotypes A, D and E were the genotypes circulating among chronic hepatitis B patients who reported to the Korle-Bu Teaching Hospital with genotype E being the most predominant and therefore constitutes an important public health concern. We recommend further epidemiological studies to understand the implication of genotype E in terms of disease progression and treatment. |
| doi_str_mv | 10.11604/pamj.supp.2016.25.1.6170 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1869964860</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1953282121</sourcerecordid><originalsourceid>FETCH-LOGICAL-c396t-dd46b8bbfe84a5ea6aa27ae7dd6b054c0b04b6556222957c64b0e7885f63141a3</originalsourceid><addsrcrecordid>eNpdkctO3TAQQK2KqlDaX6hcsemCpH7EE2cJqEBVpG7atTVxfEmukti1HST-ngQoQqxmNDrz0BxCvnJWcg6s-h5w2pdpCaEUjEMpVMlL4DV7R454I-tCg9YHr_JD8jGlPWMAWrIP5FBowZms5RHJ1y5gHvKQ6Dm9G-KS6K2bfb4PLlGc_HxLbR_9PFjavyK3zM050eiCj3lYMcz0l4-jK84Xmh3afiv2PoUh43hKz6yNeEqvepzxE3m_wzG5z8_xmPy9_PHn4rq4-X318-LsprCygVx0XQWtbtud0xUqh4AoanR110HLVGVZy6oWlAIhRKNqC1XLXK212oHkFUd5TL49zQ3R_1tcymYaknXjiLPzSzJcQ9NApYGt6MkbdO-XOK_XGd4ouT1M8JVqnigbfUrR7UyIw4Tx3nBmHtWYTY3Z1JhNjRHKcLOpWXu_PG9Y2sl1L53_XcgHHWaNqA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1953282121</pqid></control><display><type>article</type><title>Hepatitis B virus genotypes among chronic hepatitis B patients reporting at Korle-Bu teaching hospital, Accra, Ghana</title><source>African Journals Online (Open Access)</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>PubMed Central Open Access</source><creator>Dongdem, Anthony Zunuo ; Dzodzomenyo, Mawuli ; Asmah, Richard Harry ; Nyarko, Kofi Mensah ; Nortey, Priscillia ; Agyei, Adwoa ; Adjei, David Nana ; Kenu, Ernest ; Adjei, Andrew Anthony</creator><creatorcontrib>Dongdem, Anthony Zunuo ; Dzodzomenyo, Mawuli ; Asmah, Richard Harry ; Nyarko, Kofi Mensah ; Nortey, Priscillia ; Agyei, Adwoa ; Adjei, David Nana ; Kenu, Ernest ; Adjei, Andrew Anthony</creatorcontrib><description>Knowledge of hepatitis B virus (HBV) genotype is an important predictive variable which might have an impact in management and treatment of patients with chronic hepatitis B infection. In Ghana very little information is available on hepatitis B genotypes. This study was conducted to determine the distribution of HBV genotypes circulating among chronic hepatitis B patients reporting at the Korle-Bu Teaching Hospital (KBTH), Accra, Ghana.
Blood samples (10 ml) were collected from 250 consenting patients. DNA was extracted and amplified using polymerase chain reaction technique. Restriction fragment length polymorphism (RFLP) was used for the detection of genotypes.
Out of the 250 chronic hepatitis B patients who were HBsAg positive, 91 (36.4%) were males aged 29.8 ± 9.1 and 159 (63.6%) females aged 33± 12.1 years. HBV DNA was detected in 111 (44.4%) but only 58 (52%) of these were typeable. These were classified as genotype A, 8 (7.2%); genotype D, 3 (2.7%) and genotype E, 47 (42.3%). Our results did not show any association between the infecting genotype and age (X
= 0.923; p-value=0.623) or gender (X
= 0.283, p= 0.579).
Consistent with similar studies worldwide, the results suggest that genotypes A, D and E were the genotypes circulating among chronic hepatitis B patients who reported to the Korle-Bu Teaching Hospital with genotype E being the most predominant and therefore constitutes an important public health concern. We recommend further epidemiological studies to understand the implication of genotype E in terms of disease progression and treatment.</description><identifier>ISSN: 1937-8688</identifier><identifier>EISSN: 1937-8688</identifier><identifier>DOI: 10.11604/pamj.supp.2016.25.1.6170</identifier><identifier>PMID: 28210373</identifier><language>eng</language><publisher>Uganda: African Field Epidemiology Network</publisher><subject>Adolescent ; Adult ; Child ; Child, Preschool ; Cross-Sectional Studies ; DNA, Viral - blood ; Female ; Genotype ; Ghana - epidemiology ; Hepatitis B ; Hepatitis B Surface Antigens - blood ; Hepatitis B virus - genetics ; Hepatitis B virus - isolation & purification ; Hepatitis B, Chronic - epidemiology ; Hepatitis B, Chronic - virology ; Hospitals, Teaching ; Humans ; Infant ; Infant, Newborn ; Male ; Middle Aged ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Young Adult</subject><ispartof>The Pan African medical journal, 2016, Vol.25 (Suppl 1), p.5-5</ispartof><rights>Anthony Zunuo Dongdem et al. 2016. This work is licensed under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-dd46b8bbfe84a5ea6aa27ae7dd6b054c0b04b6556222957c64b0e7885f63141a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28210373$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dongdem, Anthony Zunuo</creatorcontrib><creatorcontrib>Dzodzomenyo, Mawuli</creatorcontrib><creatorcontrib>Asmah, Richard Harry</creatorcontrib><creatorcontrib>Nyarko, Kofi Mensah</creatorcontrib><creatorcontrib>Nortey, Priscillia</creatorcontrib><creatorcontrib>Agyei, Adwoa</creatorcontrib><creatorcontrib>Adjei, David Nana</creatorcontrib><creatorcontrib>Kenu, Ernest</creatorcontrib><creatorcontrib>Adjei, Andrew Anthony</creatorcontrib><title>Hepatitis B virus genotypes among chronic hepatitis B patients reporting at Korle-Bu teaching hospital, Accra, Ghana</title><title>The Pan African medical journal</title><addtitle>Pan Afr Med J</addtitle><description>Knowledge of hepatitis B virus (HBV) genotype is an important predictive variable which might have an impact in management and treatment of patients with chronic hepatitis B infection. In Ghana very little information is available on hepatitis B genotypes. This study was conducted to determine the distribution of HBV genotypes circulating among chronic hepatitis B patients reporting at the Korle-Bu Teaching Hospital (KBTH), Accra, Ghana.
Blood samples (10 ml) were collected from 250 consenting patients. DNA was extracted and amplified using polymerase chain reaction technique. Restriction fragment length polymorphism (RFLP) was used for the detection of genotypes.
Out of the 250 chronic hepatitis B patients who were HBsAg positive, 91 (36.4%) were males aged 29.8 ± 9.1 and 159 (63.6%) females aged 33± 12.1 years. HBV DNA was detected in 111 (44.4%) but only 58 (52%) of these were typeable. These were classified as genotype A, 8 (7.2%); genotype D, 3 (2.7%) and genotype E, 47 (42.3%). Our results did not show any association between the infecting genotype and age (X
= 0.923; p-value=0.623) or gender (X
= 0.283, p= 0.579).
Consistent with similar studies worldwide, the results suggest that genotypes A, D and E were the genotypes circulating among chronic hepatitis B patients who reported to the Korle-Bu Teaching Hospital with genotype E being the most predominant and therefore constitutes an important public health concern. We recommend further epidemiological studies to understand the implication of genotype E in terms of disease progression and treatment.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cross-Sectional Studies</subject><subject>DNA, Viral - blood</subject><subject>Female</subject><subject>Genotype</subject><subject>Ghana - epidemiology</subject><subject>Hepatitis B</subject><subject>Hepatitis B Surface Antigens - blood</subject><subject>Hepatitis B virus - genetics</subject><subject>Hepatitis B virus - isolation & purification</subject><subject>Hepatitis B, Chronic - epidemiology</subject><subject>Hepatitis B, Chronic - virology</subject><subject>Hospitals, Teaching</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Young Adult</subject><issn>1937-8688</issn><issn>1937-8688</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpdkctO3TAQQK2KqlDaX6hcsemCpH7EE2cJqEBVpG7atTVxfEmukti1HST-ngQoQqxmNDrz0BxCvnJWcg6s-h5w2pdpCaEUjEMpVMlL4DV7R454I-tCg9YHr_JD8jGlPWMAWrIP5FBowZms5RHJ1y5gHvKQ6Dm9G-KS6K2bfb4PLlGc_HxLbR_9PFjavyK3zM050eiCj3lYMcz0l4-jK84Xmh3afiv2PoUh43hKz6yNeEqvepzxE3m_wzG5z8_xmPy9_PHn4rq4-X318-LsprCygVx0XQWtbtud0xUqh4AoanR110HLVGVZy6oWlAIhRKNqC1XLXK212oHkFUd5TL49zQ3R_1tcymYaknXjiLPzSzJcQ9NApYGt6MkbdO-XOK_XGd4ouT1M8JVqnigbfUrR7UyIw4Tx3nBmHtWYTY3Z1JhNjRHKcLOpWXu_PG9Y2sl1L53_XcgHHWaNqA</recordid><startdate>2016</startdate><enddate>2016</enddate><creator>Dongdem, Anthony Zunuo</creator><creator>Dzodzomenyo, Mawuli</creator><creator>Asmah, Richard Harry</creator><creator>Nyarko, Kofi Mensah</creator><creator>Nortey, Priscillia</creator><creator>Agyei, Adwoa</creator><creator>Adjei, David Nana</creator><creator>Kenu, Ernest</creator><creator>Adjei, Andrew Anthony</creator><general>African Field Epidemiology Network</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>2016</creationdate><title>Hepatitis B virus genotypes among chronic hepatitis B patients reporting at Korle-Bu teaching hospital, Accra, Ghana</title><author>Dongdem, Anthony Zunuo ; Dzodzomenyo, Mawuli ; Asmah, Richard Harry ; Nyarko, Kofi Mensah ; Nortey, Priscillia ; Agyei, Adwoa ; Adjei, David Nana ; Kenu, Ernest ; Adjei, Andrew Anthony</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-dd46b8bbfe84a5ea6aa27ae7dd6b054c0b04b6556222957c64b0e7885f63141a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Cross-Sectional Studies</topic><topic>DNA, Viral - blood</topic><topic>Female</topic><topic>Genotype</topic><topic>Ghana - epidemiology</topic><topic>Hepatitis B</topic><topic>Hepatitis B Surface Antigens - blood</topic><topic>Hepatitis B virus - genetics</topic><topic>Hepatitis B virus - isolation & purification</topic><topic>Hepatitis B, Chronic - epidemiology</topic><topic>Hepatitis B, Chronic - virology</topic><topic>Hospitals, Teaching</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dongdem, Anthony Zunuo</creatorcontrib><creatorcontrib>Dzodzomenyo, Mawuli</creatorcontrib><creatorcontrib>Asmah, Richard Harry</creatorcontrib><creatorcontrib>Nyarko, Kofi Mensah</creatorcontrib><creatorcontrib>Nortey, Priscillia</creatorcontrib><creatorcontrib>Agyei, Adwoa</creatorcontrib><creatorcontrib>Adjei, David Nana</creatorcontrib><creatorcontrib>Kenu, Ernest</creatorcontrib><creatorcontrib>Adjei, Andrew Anthony</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>The Pan African medical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dongdem, Anthony Zunuo</au><au>Dzodzomenyo, Mawuli</au><au>Asmah, Richard Harry</au><au>Nyarko, Kofi Mensah</au><au>Nortey, Priscillia</au><au>Agyei, Adwoa</au><au>Adjei, David Nana</au><au>Kenu, Ernest</au><au>Adjei, Andrew Anthony</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hepatitis B virus genotypes among chronic hepatitis B patients reporting at Korle-Bu teaching hospital, Accra, Ghana</atitle><jtitle>The Pan African medical journal</jtitle><addtitle>Pan Afr Med J</addtitle><date>2016</date><risdate>2016</risdate><volume>25</volume><issue>Suppl 1</issue><spage>5</spage><epage>5</epage><pages>5-5</pages><issn>1937-8688</issn><eissn>1937-8688</eissn><abstract>Knowledge of hepatitis B virus (HBV) genotype is an important predictive variable which might have an impact in management and treatment of patients with chronic hepatitis B infection. In Ghana very little information is available on hepatitis B genotypes. This study was conducted to determine the distribution of HBV genotypes circulating among chronic hepatitis B patients reporting at the Korle-Bu Teaching Hospital (KBTH), Accra, Ghana.
Blood samples (10 ml) were collected from 250 consenting patients. DNA was extracted and amplified using polymerase chain reaction technique. Restriction fragment length polymorphism (RFLP) was used for the detection of genotypes.
Out of the 250 chronic hepatitis B patients who were HBsAg positive, 91 (36.4%) were males aged 29.8 ± 9.1 and 159 (63.6%) females aged 33± 12.1 years. HBV DNA was detected in 111 (44.4%) but only 58 (52%) of these were typeable. These were classified as genotype A, 8 (7.2%); genotype D, 3 (2.7%) and genotype E, 47 (42.3%). Our results did not show any association between the infecting genotype and age (X
= 0.923; p-value=0.623) or gender (X
= 0.283, p= 0.579).
Consistent with similar studies worldwide, the results suggest that genotypes A, D and E were the genotypes circulating among chronic hepatitis B patients who reported to the Korle-Bu Teaching Hospital with genotype E being the most predominant and therefore constitutes an important public health concern. We recommend further epidemiological studies to understand the implication of genotype E in terms of disease progression and treatment.</abstract><cop>Uganda</cop><pub>African Field Epidemiology Network</pub><pmid>28210373</pmid><doi>10.11604/pamj.supp.2016.25.1.6170</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Adult Child Child, Preschool Cross-Sectional Studies DNA, Viral - blood Female Genotype Ghana - epidemiology Hepatitis B Hepatitis B Surface Antigens - blood Hepatitis B virus - genetics Hepatitis B virus - isolation & purification Hepatitis B, Chronic - epidemiology Hepatitis B, Chronic - virology Hospitals, Teaching Humans Infant Infant, Newborn Male Middle Aged Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Young Adult |
| title | Hepatitis B virus genotypes among chronic hepatitis B patients reporting at Korle-Bu teaching hospital, Accra, Ghana |
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