Bone regeneration in a rabbit critical femoral defect by means of magnetic hydroxyapatite macroporous scaffolds
Magnetic scaffolds have recently attracted significant attention in tissue engineering due to the prospect of improving bone tissue formation by conveying soluble factors such as growth factors, hormones, and polypeptides directly to the site of implantation, as well as to the possibility of improvi...
Gespeichert in:
| Veröffentlicht in: | Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2018-02, Vol.106 (2), p.546-554 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 554 |
|---|---|
| container_issue | 2 |
| container_start_page | 546 |
| container_title | Journal of biomedical materials research. Part B, Applied biomaterials |
| container_volume | 106 |
| creator | Russo, A Bianchi, M Sartori, M Boi, M Giavaresi, G Salter, D M Jelic, M Maltarello, M C Ortolani, A Sprio, S Fini, M Tampieri, A Marcacci, M |
| description | Magnetic scaffolds have recently attracted significant attention in tissue engineering due to the prospect of improving bone tissue formation by conveying soluble factors such as growth factors, hormones, and polypeptides directly to the site of implantation, as well as to the possibility of improving implant fixation and stability. The objective of this study was to compare bone tissue formation in a preclinical rabbit model of critical femoral defect treated either with a hydroxyapatite (HA)/magnetite (90/10 wt %) or pure HA porous scaffolds at 4 and 12 weeks after implantation. The biocompatibility and osteogenic activity of the novel magnetic constructs was assessed with analysis of the amount of newly formed bone tissue and its nanomechanical properties. The osteoconductive properties of the pure HA were confirmed. The HA/magnetite scaffold was able to induce and support bone tissue formation at both experimental time points without adverse tissue reactions. Biomechanically, similar properties were obtained from nanoindentation analysis of bone formed following implantation of magnetic and control scaffolds. The results indicate that the osteoconductive properties of an HA scaffold are maintained following inclusion of a magnetic component. These provide a basis for future studies investigating the potential benefit in tissue engineering of applying magnetic stimuli to enhance bone formation. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 546-554, 2018. |
| doi_str_mv | 10.1002/jbm.b.33836 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1868694799</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1868694799</sourcerecordid><originalsourceid>FETCH-LOGICAL-c391t-e71767d2841ab8e4e68e6b5108e7ca5afee66335cebb0fc6c881140b72b20543</originalsourceid><addsrcrecordid>eNpd0c1LwzAYBvAgipvTk3cJeBGkM2naND3q8AsGXnYPSfpmdrRNTVqw_71R5w6e3hB-PLzJg9AlJUtKSHq30-1SLxkTjB-hOc3zNMlKQY8P54LN0FkIu4g5ydkpmqWCliXJ-By5B9cB9rCFDrwaatfhusMKe6V1PWDj66E2qsEWWufjrMCCGbCecAuqC9hZ3KptB1Hh96ny7nNSfcwZIN4b73rn3RhwMMpa11ThHJ1Y1QS42M8F2jw9blYvyfrt-XV1v04MK-mQQEELXlSpyKjSAjLgArjOKRFQGJUrC8A5Y7kBrYk13AhBaUZ0keqU5BlboJvf2N67jxHCINs6GGga1UHcR1LBBS-zoiwjvf5Hd270XVxO0lJEQnKSRnX7q-KbQvBgZe_rVvlJUiK_a5CxBqnlTw1RX-0zR91CdbB__86-AP-ghIw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1984790502</pqid></control><display><type>article</type><title>Bone regeneration in a rabbit critical femoral defect by means of magnetic hydroxyapatite macroporous scaffolds</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Russo, A ; Bianchi, M ; Sartori, M ; Boi, M ; Giavaresi, G ; Salter, D M ; Jelic, M ; Maltarello, M C ; Ortolani, A ; Sprio, S ; Fini, M ; Tampieri, A ; Marcacci, M</creator><creatorcontrib>Russo, A ; Bianchi, M ; Sartori, M ; Boi, M ; Giavaresi, G ; Salter, D M ; Jelic, M ; Maltarello, M C ; Ortolani, A ; Sprio, S ; Fini, M ; Tampieri, A ; Marcacci, M</creatorcontrib><description>Magnetic scaffolds have recently attracted significant attention in tissue engineering due to the prospect of improving bone tissue formation by conveying soluble factors such as growth factors, hormones, and polypeptides directly to the site of implantation, as well as to the possibility of improving implant fixation and stability. The objective of this study was to compare bone tissue formation in a preclinical rabbit model of critical femoral defect treated either with a hydroxyapatite (HA)/magnetite (90/10 wt %) or pure HA porous scaffolds at 4 and 12 weeks after implantation. The biocompatibility and osteogenic activity of the novel magnetic constructs was assessed with analysis of the amount of newly formed bone tissue and its nanomechanical properties. The osteoconductive properties of the pure HA were confirmed. The HA/magnetite scaffold was able to induce and support bone tissue formation at both experimental time points without adverse tissue reactions. Biomechanically, similar properties were obtained from nanoindentation analysis of bone formed following implantation of magnetic and control scaffolds. The results indicate that the osteoconductive properties of an HA scaffold are maintained following inclusion of a magnetic component. These provide a basis for future studies investigating the potential benefit in tissue engineering of applying magnetic stimuli to enhance bone formation. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 546-554, 2018.</description><identifier>ISSN: 1552-4973</identifier><identifier>EISSN: 1552-4981</identifier><identifier>DOI: 10.1002/jbm.b.33836</identifier><identifier>PMID: 28199046</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Animals ; Biocompatibility ; Biomechanical engineering ; Biomechanics ; Biomedical materials ; Bone growth ; Bone Regeneration - drug effects ; Bones ; Disease Models, Animal ; Durapatite - chemistry ; Durapatite - pharmacology ; Femur ; Femur - injuries ; Femur - physiology ; Ferric Compounds - chemistry ; Ferric Compounds - pharmacology ; Growth factors ; Hormones ; Hyaluronic Acid - chemistry ; Hyaluronic Acid - pharmacology ; Hydroxyapatite ; Implantation ; Magnetic fields ; Magnetic properties ; Magnetite ; Magnetite Nanoparticles - chemistry ; Male ; Materials research ; Materials science ; Mechanical properties ; Nanoindentation ; Osteoconduction ; Osteogenesis ; Osteogenesis - drug effects ; Polypeptides ; Porosity ; Rabbits ; Regeneration ; Regeneration (physiology) ; Scaffolds ; Surgical implants ; Tissue Engineering ; Tissue Scaffolds - chemistry</subject><ispartof>Journal of biomedical materials research. Part B, Applied biomaterials, 2018-02, Vol.106 (2), p.546-554</ispartof><rights>2017 Wiley Periodicals, Inc.</rights><rights>2018 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-e71767d2841ab8e4e68e6b5108e7ca5afee66335cebb0fc6c881140b72b20543</citedby><cites>FETCH-LOGICAL-c391t-e71767d2841ab8e4e68e6b5108e7ca5afee66335cebb0fc6c881140b72b20543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28199046$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Russo, A</creatorcontrib><creatorcontrib>Bianchi, M</creatorcontrib><creatorcontrib>Sartori, M</creatorcontrib><creatorcontrib>Boi, M</creatorcontrib><creatorcontrib>Giavaresi, G</creatorcontrib><creatorcontrib>Salter, D M</creatorcontrib><creatorcontrib>Jelic, M</creatorcontrib><creatorcontrib>Maltarello, M C</creatorcontrib><creatorcontrib>Ortolani, A</creatorcontrib><creatorcontrib>Sprio, S</creatorcontrib><creatorcontrib>Fini, M</creatorcontrib><creatorcontrib>Tampieri, A</creatorcontrib><creatorcontrib>Marcacci, M</creatorcontrib><title>Bone regeneration in a rabbit critical femoral defect by means of magnetic hydroxyapatite macroporous scaffolds</title><title>Journal of biomedical materials research. Part B, Applied biomaterials</title><addtitle>J Biomed Mater Res B Appl Biomater</addtitle><description>Magnetic scaffolds have recently attracted significant attention in tissue engineering due to the prospect of improving bone tissue formation by conveying soluble factors such as growth factors, hormones, and polypeptides directly to the site of implantation, as well as to the possibility of improving implant fixation and stability. The objective of this study was to compare bone tissue formation in a preclinical rabbit model of critical femoral defect treated either with a hydroxyapatite (HA)/magnetite (90/10 wt %) or pure HA porous scaffolds at 4 and 12 weeks after implantation. The biocompatibility and osteogenic activity of the novel magnetic constructs was assessed with analysis of the amount of newly formed bone tissue and its nanomechanical properties. The osteoconductive properties of the pure HA were confirmed. The HA/magnetite scaffold was able to induce and support bone tissue formation at both experimental time points without adverse tissue reactions. Biomechanically, similar properties were obtained from nanoindentation analysis of bone formed following implantation of magnetic and control scaffolds. The results indicate that the osteoconductive properties of an HA scaffold are maintained following inclusion of a magnetic component. These provide a basis for future studies investigating the potential benefit in tissue engineering of applying magnetic stimuli to enhance bone formation. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 546-554, 2018.</description><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biomechanical engineering</subject><subject>Biomechanics</subject><subject>Biomedical materials</subject><subject>Bone growth</subject><subject>Bone Regeneration - drug effects</subject><subject>Bones</subject><subject>Disease Models, Animal</subject><subject>Durapatite - chemistry</subject><subject>Durapatite - pharmacology</subject><subject>Femur</subject><subject>Femur - injuries</subject><subject>Femur - physiology</subject><subject>Ferric Compounds - chemistry</subject><subject>Ferric Compounds - pharmacology</subject><subject>Growth factors</subject><subject>Hormones</subject><subject>Hyaluronic Acid - chemistry</subject><subject>Hyaluronic Acid - pharmacology</subject><subject>Hydroxyapatite</subject><subject>Implantation</subject><subject>Magnetic fields</subject><subject>Magnetic properties</subject><subject>Magnetite</subject><subject>Magnetite Nanoparticles - chemistry</subject><subject>Male</subject><subject>Materials research</subject><subject>Materials science</subject><subject>Mechanical properties</subject><subject>Nanoindentation</subject><subject>Osteoconduction</subject><subject>Osteogenesis</subject><subject>Osteogenesis - drug effects</subject><subject>Polypeptides</subject><subject>Porosity</subject><subject>Rabbits</subject><subject>Regeneration</subject><subject>Regeneration (physiology)</subject><subject>Scaffolds</subject><subject>Surgical implants</subject><subject>Tissue Engineering</subject><subject>Tissue Scaffolds - chemistry</subject><issn>1552-4973</issn><issn>1552-4981</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0c1LwzAYBvAgipvTk3cJeBGkM2naND3q8AsGXnYPSfpmdrRNTVqw_71R5w6e3hB-PLzJg9AlJUtKSHq30-1SLxkTjB-hOc3zNMlKQY8P54LN0FkIu4g5ydkpmqWCliXJ-By5B9cB9rCFDrwaatfhusMKe6V1PWDj66E2qsEWWufjrMCCGbCecAuqC9hZ3KptB1Hh96ny7nNSfcwZIN4b73rn3RhwMMpa11ThHJ1Y1QS42M8F2jw9blYvyfrt-XV1v04MK-mQQEELXlSpyKjSAjLgArjOKRFQGJUrC8A5Y7kBrYk13AhBaUZ0keqU5BlboJvf2N67jxHCINs6GGga1UHcR1LBBS-zoiwjvf5Hd270XVxO0lJEQnKSRnX7q-KbQvBgZe_rVvlJUiK_a5CxBqnlTw1RX-0zR91CdbB__86-AP-ghIw</recordid><startdate>20180201</startdate><enddate>20180201</enddate><creator>Russo, A</creator><creator>Bianchi, M</creator><creator>Sartori, M</creator><creator>Boi, M</creator><creator>Giavaresi, G</creator><creator>Salter, D M</creator><creator>Jelic, M</creator><creator>Maltarello, M C</creator><creator>Ortolani, A</creator><creator>Sprio, S</creator><creator>Fini, M</creator><creator>Tampieri, A</creator><creator>Marcacci, M</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20180201</creationdate><title>Bone regeneration in a rabbit critical femoral defect by means of magnetic hydroxyapatite macroporous scaffolds</title><author>Russo, A ; Bianchi, M ; Sartori, M ; Boi, M ; Giavaresi, G ; Salter, D M ; Jelic, M ; Maltarello, M C ; Ortolani, A ; Sprio, S ; Fini, M ; Tampieri, A ; Marcacci, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-e71767d2841ab8e4e68e6b5108e7ca5afee66335cebb0fc6c881140b72b20543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Biocompatibility</topic><topic>Biomechanical engineering</topic><topic>Biomechanics</topic><topic>Biomedical materials</topic><topic>Bone growth</topic><topic>Bone Regeneration - drug effects</topic><topic>Bones</topic><topic>Disease Models, Animal</topic><topic>Durapatite - chemistry</topic><topic>Durapatite - pharmacology</topic><topic>Femur</topic><topic>Femur - injuries</topic><topic>Femur - physiology</topic><topic>Ferric Compounds - chemistry</topic><topic>Ferric Compounds - pharmacology</topic><topic>Growth factors</topic><topic>Hormones</topic><topic>Hyaluronic Acid - chemistry</topic><topic>Hyaluronic Acid - pharmacology</topic><topic>Hydroxyapatite</topic><topic>Implantation</topic><topic>Magnetic fields</topic><topic>Magnetic properties</topic><topic>Magnetite</topic><topic>Magnetite Nanoparticles - chemistry</topic><topic>Male</topic><topic>Materials research</topic><topic>Materials science</topic><topic>Mechanical properties</topic><topic>Nanoindentation</topic><topic>Osteoconduction</topic><topic>Osteogenesis</topic><topic>Osteogenesis - drug effects</topic><topic>Polypeptides</topic><topic>Porosity</topic><topic>Rabbits</topic><topic>Regeneration</topic><topic>Regeneration (physiology)</topic><topic>Scaffolds</topic><topic>Surgical implants</topic><topic>Tissue Engineering</topic><topic>Tissue Scaffolds - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Russo, A</creatorcontrib><creatorcontrib>Bianchi, M</creatorcontrib><creatorcontrib>Sartori, M</creatorcontrib><creatorcontrib>Boi, M</creatorcontrib><creatorcontrib>Giavaresi, G</creatorcontrib><creatorcontrib>Salter, D M</creatorcontrib><creatorcontrib>Jelic, M</creatorcontrib><creatorcontrib>Maltarello, M C</creatorcontrib><creatorcontrib>Ortolani, A</creatorcontrib><creatorcontrib>Sprio, S</creatorcontrib><creatorcontrib>Fini, M</creatorcontrib><creatorcontrib>Tampieri, A</creatorcontrib><creatorcontrib>Marcacci, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research. Part B, Applied biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Russo, A</au><au>Bianchi, M</au><au>Sartori, M</au><au>Boi, M</au><au>Giavaresi, G</au><au>Salter, D M</au><au>Jelic, M</au><au>Maltarello, M C</au><au>Ortolani, A</au><au>Sprio, S</au><au>Fini, M</au><au>Tampieri, A</au><au>Marcacci, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bone regeneration in a rabbit critical femoral defect by means of magnetic hydroxyapatite macroporous scaffolds</atitle><jtitle>Journal of biomedical materials research. Part B, Applied biomaterials</jtitle><addtitle>J Biomed Mater Res B Appl Biomater</addtitle><date>2018-02-01</date><risdate>2018</risdate><volume>106</volume><issue>2</issue><spage>546</spage><epage>554</epage><pages>546-554</pages><issn>1552-4973</issn><eissn>1552-4981</eissn><abstract>Magnetic scaffolds have recently attracted significant attention in tissue engineering due to the prospect of improving bone tissue formation by conveying soluble factors such as growth factors, hormones, and polypeptides directly to the site of implantation, as well as to the possibility of improving implant fixation and stability. The objective of this study was to compare bone tissue formation in a preclinical rabbit model of critical femoral defect treated either with a hydroxyapatite (HA)/magnetite (90/10 wt %) or pure HA porous scaffolds at 4 and 12 weeks after implantation. The biocompatibility and osteogenic activity of the novel magnetic constructs was assessed with analysis of the amount of newly formed bone tissue and its nanomechanical properties. The osteoconductive properties of the pure HA were confirmed. The HA/magnetite scaffold was able to induce and support bone tissue formation at both experimental time points without adverse tissue reactions. Biomechanically, similar properties were obtained from nanoindentation analysis of bone formed following implantation of magnetic and control scaffolds. The results indicate that the osteoconductive properties of an HA scaffold are maintained following inclusion of a magnetic component. These provide a basis for future studies investigating the potential benefit in tissue engineering of applying magnetic stimuli to enhance bone formation. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 546-554, 2018.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>28199046</pmid><doi>10.1002/jbm.b.33836</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1552-4973 |
| ispartof | Journal of biomedical materials research. Part B, Applied biomaterials, 2018-02, Vol.106 (2), p.546-554 |
| issn | 1552-4973 1552-4981 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1868694799 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Biocompatibility Biomechanical engineering Biomechanics Biomedical materials Bone growth Bone Regeneration - drug effects Bones Disease Models, Animal Durapatite - chemistry Durapatite - pharmacology Femur Femur - injuries Femur - physiology Ferric Compounds - chemistry Ferric Compounds - pharmacology Growth factors Hormones Hyaluronic Acid - chemistry Hyaluronic Acid - pharmacology Hydroxyapatite Implantation Magnetic fields Magnetic properties Magnetite Magnetite Nanoparticles - chemistry Male Materials research Materials science Mechanical properties Nanoindentation Osteoconduction Osteogenesis Osteogenesis - drug effects Polypeptides Porosity Rabbits Regeneration Regeneration (physiology) Scaffolds Surgical implants Tissue Engineering Tissue Scaffolds - chemistry |
| title | Bone regeneration in a rabbit critical femoral defect by means of magnetic hydroxyapatite macroporous scaffolds |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T17%3A31%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bone%20regeneration%20in%20a%20rabbit%20critical%20femoral%20defect%20by%20means%20of%20magnetic%20hydroxyapatite%20macroporous%20scaffolds&rft.jtitle=Journal%20of%20biomedical%20materials%20research.%20Part%20B,%20Applied%20biomaterials&rft.au=Russo,%20A&rft.date=2018-02-01&rft.volume=106&rft.issue=2&rft.spage=546&rft.epage=554&rft.pages=546-554&rft.issn=1552-4973&rft.eissn=1552-4981&rft_id=info:doi/10.1002/jbm.b.33836&rft_dat=%3Cproquest_cross%3E1868694799%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1984790502&rft_id=info:pmid/28199046&rfr_iscdi=true |