Parallel experimental design and multivariate analysis provides efficient screening of cell culture media supplements to improve biosimilar product quality

ABSTRACT Rational and high‐throughput optimization of mammalian cell culture media has a great potential to modulate recombinant protein product quality. We present a process design method based on parallel design‐of‐experiment (DoE) of CHO fed‐batch cultures in 96‐deepwell plates to modulate monocl...

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Veröffentlicht in:	Biotechnology and bioengineering 2017-07, Vol.114 (7), p.1448-1458
Hauptverfasser:	Brühlmann, David, Sokolov, Michael, Butté, Alessandro, Sauer, Markus, Hemberger, Jürgen, Souquet, Jonathan, Broly, Hervé, Jordan, Martin
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal antibody glycosylation Batch Cell Culture Techniques - methods Batch Cell Culture Techniques - standards Batch culture Bioengineering Biosimilar Pharmaceuticals - metabolism Biosimilar Pharmaceuticals - standards Cell culture CHO cell culture CHO Cells Cricetulus Culture media Culture Media - chemistry Culture Media - metabolism Culture Media - standards Decision analysis Decision trees Design factors Design of experiments Dietary supplements DoE Experimental design Glycosylation High-Throughput Screening Assays - methods High-Throughput Screening Assays - standards high‐throughput Modulators Monoclonal antibodies Multivariate Analysis Optimization Pattern analysis Plates Principal Component Analysis Principal components analysis Protein Engineering - methods Protein Engineering - standards Quality Control Recombinant Proteins - biosynthesis Recombinant Proteins - standards Risk reduction Screening sequential process development Specifications Temperature effects Tissue Array Analysis - methods Tubes
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