Rapid Fluorescence Polarization Immunoassay for the Mycotoxin Deoxynivalenol in Wheat

Rapid Fluorescence Polarization Immunoassay for the Mycotoxin Deoxynivalenol in Wheat

The fungus Fusarium graminearum, a pathogen of both wheat and maize, produces a toxin, deoxynivalenol (DON), that causes disease in livestock. A rapid test for DON in wheat was developed using the principle of fluorescence polarization (FP) immunoassay. The assay was based on the competition between...

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Veröffentlicht in:Journal of agricultural and food chemistry 2002-03, Vol.50 (7), p.1827-1832
Hauptverfasser: Maragos, Chris M, Plattner, Ronald D
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Sprache:eng
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Biological and medical sciences
Chromatography, High Pressure Liquid
Fluorescence Polarization Immunoassay - methods
Food Contamination
Food industries
Food toxicology
Fundamental and applied biological sciences. Psychology
Sensitivity and Specificity
Trichothecenes - analysis
Triticum - chemistry
Zea mays - chemistry
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container_issue 7
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container_title Journal of agricultural and food chemistry
container_volume 50
creator Maragos, Chris M
Plattner, Ronald D
description The fungus Fusarium graminearum, a pathogen of both wheat and maize, produces a toxin, deoxynivalenol (DON), that causes disease in livestock. A rapid test for DON in wheat was developed using the principle of fluorescence polarization (FP) immunoassay. The assay was based on the competition between DON and a novel DON-fluorescein tracer (DON-FL2) for a DON-specific monoclonal antibody in solution. The method, which is a substantial improvement over our previous DON FP immunoassay, combined a rapid (3 min) extraction step with a rapid (2 min) detection step. A series of naturally contaminated wheat and maize samples were analyzed by both FP immunoassay and liquid chromatography (HPLC−UV). For wheat the HPLC−UV and FP methods agreed well (linear regression r 2 = 0.936), but for maize the two methods did not (r  2 = 0.849). We conclude that the FP method is useful for screening wheat, but not maize, for DON. Keywords: Deoxynivalenol; vomitoxin; fluorescence polarization; immunoassay
doi_str_mv 10.1021/jf011487d
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Agric. Food Chem</addtitle><description>The fungus Fusarium graminearum, a pathogen of both wheat and maize, produces a toxin, deoxynivalenol (DON), that causes disease in livestock. A rapid test for DON in wheat was developed using the principle of fluorescence polarization (FP) immunoassay. The assay was based on the competition between DON and a novel DON-fluorescein tracer (DON-FL2) for a DON-specific monoclonal antibody in solution. The method, which is a substantial improvement over our previous DON FP immunoassay, combined a rapid (3 min) extraction step with a rapid (2 min) detection step. A series of naturally contaminated wheat and maize samples were analyzed by both FP immunoassay and liquid chromatography (HPLC−UV). For wheat the HPLC−UV and FP methods agreed well (linear regression r 2 = 0.936), but for maize the two methods did not (r  2 = 0.849). We conclude that the FP method is useful for screening wheat, but not maize, for DON. 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subjects Biological and medical sciences
Chromatography, High Pressure Liquid
Fluorescence Polarization Immunoassay - methods
Food Contamination
Food industries
Food toxicology
Fundamental and applied biological sciences. Psychology
Sensitivity and Specificity
Trichothecenes - analysis
Triticum - chemistry
Zea mays - chemistry
title Rapid Fluorescence Polarization Immunoassay for the Mycotoxin Deoxynivalenol in Wheat
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