Heat shock 70 kDa protein cognate 5 involved in WSSV toleration of Litopenaeus vannamei
The expression levels of 97 unigenes encoding heat shock proteins of Litopenaeus vannamei was scanned, and ten of them were significantly induced by white spot syndrome virus (WSSV). Among these genes, heat shock 70 kDa protein cognate 5 (LvHSC70-5) was upregulated to the highest extent and subjecte...
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| Veröffentlicht in: | Developmental and comparative immunology 2017-07, Vol.72, p.9-20 |
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| container_title | Developmental and comparative immunology |
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| creator | Yuan, Kai Yuan, Feng-Hua He, Hong-Hui Bi, Hai-Tao Weng, Shao-Ping He, Jian-Guo Chen, Yi-Hong |
| description | The expression levels of 97 unigenes encoding heat shock proteins of Litopenaeus vannamei was scanned, and ten of them were significantly induced by white spot syndrome virus (WSSV). Among these genes, heat shock 70 kDa protein cognate 5 (LvHSC70-5) was upregulated to the highest extent and subjected to further studies. Subcellular localization assay revealed that LvHSC70-5 was located in the mitochondria. Aside from WSSV infection, unfolded protein response activation and thermal stress could also upregulate LvHSC70-5. Results of reporter gene assay demonstrated that promoter of LvHSC70-5 was activated by L. vannamei heat shock factor protein 1, activating transcription factor 4 and thermal stress. A decrease in the expression of LvHSC70-5 could reduce the aggregation of proteins in hemocytes and the cumulative mortality of WSSV-infected L. vannamei. LvHSC70-5 in L. vannamei hemocytes was upregulated by mild thermal stress. In addition, mild thermal stress, decreased the copy number of WSSV in shrimp muscle and the cumulative mortality of WSSV-infected L. vannamei. Therefore, collecting results suggested that LvHSC70-5 should be involved in WSSV toleration of shrimp L. vannamei.
•LvHSC70-5 is upregulated by UPR activation and thermal stress in L. vannamei.•LvHSC70-5 is activated by L. vannamei HSF1, ATF4 and thermal stress.•LvHSC70-5 reduces the aggregation of proteins and the cumulative mortality of WSSV-infected shrimp.•Mild thermal stresses decrease the copy number of WSSV and the cumulative mortality of WSSV-infected L. vannamei. |
| doi_str_mv | 10.1016/j.dci.2017.02.003 |
| format | Article |
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•LvHSC70-5 is upregulated by UPR activation and thermal stress in L. vannamei.•LvHSC70-5 is activated by L. vannamei HSF1, ATF4 and thermal stress.•LvHSC70-5 reduces the aggregation of proteins and the cumulative mortality of WSSV-infected shrimp.•Mild thermal stresses decrease the copy number of WSSV and the cumulative mortality of WSSV-infected L. vannamei.</description><identifier>ISSN: 0145-305X</identifier><identifier>EISSN: 1879-0089</identifier><identifier>DOI: 10.1016/j.dci.2017.02.003</identifier><identifier>PMID: 28193450</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Activating transcription factor 4 ; Animals ; Arthropod Proteins - genetics ; Arthropod Proteins - metabolism ; Cloning, Molecular ; Copy number ; DNA Virus Infections - immunology ; ER-stress ; Heat shock factors ; Heat shock protein cognate 5 ; Heat shock proteins ; Heat-Shock Response ; Hemocytes ; Hemocytes - immunology ; Hot Temperature - adverse effects ; HSP70 Heat-Shock Proteins - genetics ; HSP70 Heat-Shock Proteins - metabolism ; Litopenaeus vannamei ; Localization ; Mitochondria ; Mitochondria - metabolism ; Mortality ; Muscles ; Muscles - virology ; Penaeidae - immunology ; Phylogeny ; Protein folding ; Reporter gene ; Shellfish ; Thermal stress ; Transcription Factor 4 - genetics ; Transcription Factor 4 - metabolism ; Transcriptional Activation ; Unfolded Protein Response ; Up-Regulation ; Viral Load ; Viruses ; White spot syndrome ; White spot syndrome virus ; White spot syndrome virus 1 - physiology</subject><ispartof>Developmental and comparative immunology, 2017-07, Vol.72, p.9-20</ispartof><rights>2017 Elsevier Ltd</rights><rights>Copyright © 2017 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Science Ltd. Jul 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-3e396f2f900ae88f254875d17330a942b61e76da480c3c141a209fae49bbbdf33</citedby><cites>FETCH-LOGICAL-c381t-3e396f2f900ae88f254875d17330a942b61e76da480c3c141a209fae49bbbdf33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0145305X16303391$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28193450$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yuan, Kai</creatorcontrib><creatorcontrib>Yuan, Feng-Hua</creatorcontrib><creatorcontrib>He, Hong-Hui</creatorcontrib><creatorcontrib>Bi, Hai-Tao</creatorcontrib><creatorcontrib>Weng, Shao-Ping</creatorcontrib><creatorcontrib>He, Jian-Guo</creatorcontrib><creatorcontrib>Chen, Yi-Hong</creatorcontrib><title>Heat shock 70 kDa protein cognate 5 involved in WSSV toleration of Litopenaeus vannamei</title><title>Developmental and comparative immunology</title><addtitle>Dev Comp Immunol</addtitle><description>The expression levels of 97 unigenes encoding heat shock proteins of Litopenaeus vannamei was scanned, and ten of them were significantly induced by white spot syndrome virus (WSSV). Among these genes, heat shock 70 kDa protein cognate 5 (LvHSC70-5) was upregulated to the highest extent and subjected to further studies. Subcellular localization assay revealed that LvHSC70-5 was located in the mitochondria. Aside from WSSV infection, unfolded protein response activation and thermal stress could also upregulate LvHSC70-5. Results of reporter gene assay demonstrated that promoter of LvHSC70-5 was activated by L. vannamei heat shock factor protein 1, activating transcription factor 4 and thermal stress. A decrease in the expression of LvHSC70-5 could reduce the aggregation of proteins in hemocytes and the cumulative mortality of WSSV-infected L. vannamei. LvHSC70-5 in L. vannamei hemocytes was upregulated by mild thermal stress. In addition, mild thermal stress, decreased the copy number of WSSV in shrimp muscle and the cumulative mortality of WSSV-infected L. vannamei. Therefore, collecting results suggested that LvHSC70-5 should be involved in WSSV toleration of shrimp L. vannamei.
•LvHSC70-5 is upregulated by UPR activation and thermal stress in L. vannamei.•LvHSC70-5 is activated by L. vannamei HSF1, ATF4 and thermal stress.•LvHSC70-5 reduces the aggregation of proteins and the cumulative mortality of WSSV-infected shrimp.•Mild thermal stresses decrease the copy number of WSSV and the cumulative mortality of WSSV-infected L. vannamei.</description><subject>Activating transcription factor 4</subject><subject>Animals</subject><subject>Arthropod Proteins - genetics</subject><subject>Arthropod Proteins - metabolism</subject><subject>Cloning, Molecular</subject><subject>Copy number</subject><subject>DNA Virus Infections - immunology</subject><subject>ER-stress</subject><subject>Heat shock factors</subject><subject>Heat shock protein cognate 5</subject><subject>Heat shock proteins</subject><subject>Heat-Shock Response</subject><subject>Hemocytes</subject><subject>Hemocytes - immunology</subject><subject>Hot Temperature - adverse effects</subject><subject>HSP70 Heat-Shock Proteins - genetics</subject><subject>HSP70 Heat-Shock Proteins - metabolism</subject><subject>Litopenaeus vannamei</subject><subject>Localization</subject><subject>Mitochondria</subject><subject>Mitochondria - metabolism</subject><subject>Mortality</subject><subject>Muscles</subject><subject>Muscles - virology</subject><subject>Penaeidae - immunology</subject><subject>Phylogeny</subject><subject>Protein folding</subject><subject>Reporter gene</subject><subject>Shellfish</subject><subject>Thermal stress</subject><subject>Transcription Factor 4 - genetics</subject><subject>Transcription Factor 4 - metabolism</subject><subject>Transcriptional Activation</subject><subject>Unfolded Protein Response</subject><subject>Up-Regulation</subject><subject>Viral Load</subject><subject>Viruses</subject><subject>White spot syndrome</subject><subject>White spot syndrome virus</subject><subject>White spot syndrome virus 1 - physiology</subject><issn>0145-305X</issn><issn>1879-0089</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1u1DAQxy0EotvCA3BBlrhwSRh_JHHECRVKkVbiUD56sxxnAt5m7a3trNS34Vn6ZLja0gOHzmXm8Jv_jH6EvGJQM2Dtu009WldzYF0NvAYQT8iKqa6vAFT_lKyAyaYS0FwekeOUNlBKMXhOjrhivZANrMjlOZpM0-9gr2gHt3-uPhq6iyGj89SGX95kpA11fh_mPY5loD8vLn7QHGaMJrvgaZjo2uWwQ29wSXRvvDdbdC_Is8nMCV_e9xPy_ezTt9Pzav3185fTD-vKCsVyJVD07cSnHsCgUhNvpOqakXVCgOklH1qGXTsaqcAKyyQzHPrJoOyHYRgnIU7I20Nu-fp6wZT11iWL82w8hiVpploleskkFPTNf-gmLNGX7zQHXi4UqV2h2IGyMaQUcdK76LYm3mgG-k673uiiXd9p18B10V52Xt8nL8MWx4eNf54L8P4AYFGxdxh1sg69xdFFtFmPwT0S_xdftJFL</recordid><startdate>201707</startdate><enddate>201707</enddate><creator>Yuan, Kai</creator><creator>Yuan, Feng-Hua</creator><creator>He, Hong-Hui</creator><creator>Bi, Hai-Tao</creator><creator>Weng, Shao-Ping</creator><creator>He, Jian-Guo</creator><creator>Chen, Yi-Hong</creator><general>Elsevier Ltd</general><general>Elsevier Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>201707</creationdate><title>Heat shock 70 kDa protein cognate 5 involved in WSSV toleration of Litopenaeus vannamei</title><author>Yuan, Kai ; Yuan, Feng-Hua ; He, Hong-Hui ; Bi, Hai-Tao ; Weng, Shao-Ping ; He, Jian-Guo ; Chen, Yi-Hong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-3e396f2f900ae88f254875d17330a942b61e76da480c3c141a209fae49bbbdf33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Activating transcription factor 4</topic><topic>Animals</topic><topic>Arthropod Proteins - genetics</topic><topic>Arthropod Proteins - metabolism</topic><topic>Cloning, Molecular</topic><topic>Copy number</topic><topic>DNA Virus Infections - immunology</topic><topic>ER-stress</topic><topic>Heat shock factors</topic><topic>Heat shock protein cognate 5</topic><topic>Heat shock proteins</topic><topic>Heat-Shock Response</topic><topic>Hemocytes</topic><topic>Hemocytes - immunology</topic><topic>Hot Temperature - adverse effects</topic><topic>HSP70 Heat-Shock Proteins - genetics</topic><topic>HSP70 Heat-Shock Proteins - metabolism</topic><topic>Litopenaeus vannamei</topic><topic>Localization</topic><topic>Mitochondria</topic><topic>Mitochondria - metabolism</topic><topic>Mortality</topic><topic>Muscles</topic><topic>Muscles - virology</topic><topic>Penaeidae - immunology</topic><topic>Phylogeny</topic><topic>Protein folding</topic><topic>Reporter gene</topic><topic>Shellfish</topic><topic>Thermal stress</topic><topic>Transcription Factor 4 - genetics</topic><topic>Transcription Factor 4 - metabolism</topic><topic>Transcriptional Activation</topic><topic>Unfolded Protein Response</topic><topic>Up-Regulation</topic><topic>Viral Load</topic><topic>Viruses</topic><topic>White spot syndrome</topic><topic>White spot syndrome virus</topic><topic>White spot syndrome virus 1 - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yuan, Kai</creatorcontrib><creatorcontrib>Yuan, Feng-Hua</creatorcontrib><creatorcontrib>He, Hong-Hui</creatorcontrib><creatorcontrib>Bi, Hai-Tao</creatorcontrib><creatorcontrib>Weng, Shao-Ping</creatorcontrib><creatorcontrib>He, Jian-Guo</creatorcontrib><creatorcontrib>Chen, Yi-Hong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental and comparative immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yuan, Kai</au><au>Yuan, Feng-Hua</au><au>He, Hong-Hui</au><au>Bi, Hai-Tao</au><au>Weng, Shao-Ping</au><au>He, Jian-Guo</au><au>Chen, Yi-Hong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heat shock 70 kDa protein cognate 5 involved in WSSV toleration of Litopenaeus vannamei</atitle><jtitle>Developmental and comparative immunology</jtitle><addtitle>Dev Comp Immunol</addtitle><date>2017-07</date><risdate>2017</risdate><volume>72</volume><spage>9</spage><epage>20</epage><pages>9-20</pages><issn>0145-305X</issn><eissn>1879-0089</eissn><abstract>The expression levels of 97 unigenes encoding heat shock proteins of Litopenaeus vannamei was scanned, and ten of them were significantly induced by white spot syndrome virus (WSSV). Among these genes, heat shock 70 kDa protein cognate 5 (LvHSC70-5) was upregulated to the highest extent and subjected to further studies. Subcellular localization assay revealed that LvHSC70-5 was located in the mitochondria. Aside from WSSV infection, unfolded protein response activation and thermal stress could also upregulate LvHSC70-5. Results of reporter gene assay demonstrated that promoter of LvHSC70-5 was activated by L. vannamei heat shock factor protein 1, activating transcription factor 4 and thermal stress. A decrease in the expression of LvHSC70-5 could reduce the aggregation of proteins in hemocytes and the cumulative mortality of WSSV-infected L. vannamei. LvHSC70-5 in L. vannamei hemocytes was upregulated by mild thermal stress. In addition, mild thermal stress, decreased the copy number of WSSV in shrimp muscle and the cumulative mortality of WSSV-infected L. vannamei. Therefore, collecting results suggested that LvHSC70-5 should be involved in WSSV toleration of shrimp L. vannamei.
•LvHSC70-5 is upregulated by UPR activation and thermal stress in L. vannamei.•LvHSC70-5 is activated by L. vannamei HSF1, ATF4 and thermal stress.•LvHSC70-5 reduces the aggregation of proteins and the cumulative mortality of WSSV-infected shrimp.•Mild thermal stresses decrease the copy number of WSSV and the cumulative mortality of WSSV-infected L. vannamei.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>28193450</pmid><doi>10.1016/j.dci.2017.02.003</doi><tpages>12</tpages></addata></record> |
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| subjects | Activating transcription factor 4 Animals Arthropod Proteins - genetics Arthropod Proteins - metabolism Cloning, Molecular Copy number DNA Virus Infections - immunology ER-stress Heat shock factors Heat shock protein cognate 5 Heat shock proteins Heat-Shock Response Hemocytes Hemocytes - immunology Hot Temperature - adverse effects HSP70 Heat-Shock Proteins - genetics HSP70 Heat-Shock Proteins - metabolism Litopenaeus vannamei Localization Mitochondria Mitochondria - metabolism Mortality Muscles Muscles - virology Penaeidae - immunology Phylogeny Protein folding Reporter gene Shellfish Thermal stress Transcription Factor 4 - genetics Transcription Factor 4 - metabolism Transcriptional Activation Unfolded Protein Response Up-Regulation Viral Load Viruses White spot syndrome White spot syndrome virus White spot syndrome virus 1 - physiology |
| title | Heat shock 70 kDa protein cognate 5 involved in WSSV toleration of Litopenaeus vannamei |
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