Competitive binding experiments can reduce the false positive results of affinity-based ultrafiltration-HPLC: A case study for identification of potent xanthine oxidase inhibitors from Perilla frutescens extract

•Ultrafiltration-HPLC combined with competitive experiment can eliminate false positive result.•The limitations of proposed strategy were discussed.•Relationship of TBD, binding affinity and binding specificity was discussed.•Relationship of SBD, binding affinity and binding specificity was discusse...

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