Establishment of a murine model of cerebral malaria in KunMing mice infected with Plasmodium berghei ANKA
Malaria remains one of the most devastating diseases. Cerebral malaria (CM) is a severe complication of Plasmodium falciparum infection resulting in high mortality and morbidity worldwide. Analysis of precise mechanisms of CM in humans is difficult for ethical reasons and animal models of CM have be...
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| Veröffentlicht in: | Parasitology 2016-10, Vol.143 (12), p.1672-1680 |
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| creator | DING, YAN XU, WENYUE ZHOU, TAOLI LIU, TAIPING ZHENG, HONG FU, YONG |
| description | Malaria remains one of the most devastating diseases. Cerebral malaria (CM) is a severe complication of Plasmodium falciparum infection resulting in high mortality and morbidity worldwide. Analysis of precise mechanisms of CM in humans is difficult for ethical reasons and animal models of CM have been employed to study malaria pathogenesis. Here, we describe a new experimental cerebral malaria (ECM) model with Plasmodium berghei ANKA infection in KunMing (KM) mice. KM mice developed ECM after blood-stage or sporozoites infection, and the development of ECM in KM mice has a dose-dependent relationship with sporozoites inoculums. Histopathological findings revealed important features associated with ECM, including accumulation of mononuclear cells and red blood cells in brain microvascular, and brain parenchymal haemorrhages. Blood–brain barrier (BBB) examination showed that BBB disruption was present in infected KM mice when displaying clinical signs of CM. In vivo bioluminescent imaging experiment indicated that parasitized red blood cells accumulated in most vital organs including heart, lung, spleen, kidney, liver and brain. The levels of inflammatory cytokines interferon-gamma, tumour necrosis factor-alpha, interleukin (IL)-17, IL-12, IL-6 and IL-10 were all remarkably increased in KM mice infected with P. berghei ANKA. This study indicates that P. berghei ANKA infection in KM mice can be used as ECM model to extend further research on genetic, pharmacological and vaccine studies of CM. |
| doi_str_mv | 10.1017/S0031182016001475 |
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Cerebral malaria (CM) is a severe complication of Plasmodium falciparum infection resulting in high mortality and morbidity worldwide. Analysis of precise mechanisms of CM in humans is difficult for ethical reasons and animal models of CM have been employed to study malaria pathogenesis. Here, we describe a new experimental cerebral malaria (ECM) model with Plasmodium berghei ANKA infection in KunMing (KM) mice. KM mice developed ECM after blood-stage or sporozoites infection, and the development of ECM in KM mice has a dose-dependent relationship with sporozoites inoculums. Histopathological findings revealed important features associated with ECM, including accumulation of mononuclear cells and red blood cells in brain microvascular, and brain parenchymal haemorrhages. Blood–brain barrier (BBB) examination showed that BBB disruption was present in infected KM mice when displaying clinical signs of CM. In vivo bioluminescent imaging experiment indicated that parasitized red blood cells accumulated in most vital organs including heart, lung, spleen, kidney, liver and brain. The levels of inflammatory cytokines interferon-gamma, tumour necrosis factor-alpha, interleukin (IL)-17, IL-12, IL-6 and IL-10 were all remarkably increased in KM mice infected with P. berghei ANKA. This study indicates that P. berghei ANKA infection in KM mice can be used as ECM model to extend further research on genetic, pharmacological and vaccine studies of CM.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182016001475</identifier><identifier>PMID: 27574013</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Animal models ; Animal Structures - parasitology ; Animal Structures - pathology ; Animals ; Disease Models, Animal ; Erythrocytes ; Histocytochemistry ; Malaria ; Malaria, Cerebral - parasitology ; Malaria, Cerebral - pathology ; Mice ; Microscopy ; Plasmodium berghei ; Plasmodium berghei - growth & development ; Plasmodium berghei - pathogenicity ; Plasmodium falciparum ; Vector-borne diseases</subject><ispartof>Parasitology, 2016-10, Vol.143 (12), p.1672-1680</ispartof><rights>Copyright © Cambridge University Press 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-22f08c1fd9d3aaf7a328db2b10b0ab32184862e9ad6b21c18d34d6d6826dcb843</citedby><cites>FETCH-LOGICAL-c406t-22f08c1fd9d3aaf7a328db2b10b0ab32184862e9ad6b21c18d34d6d6826dcb843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182016001475/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,780,784,27923,27924,55627</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27574013$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DING, YAN</creatorcontrib><creatorcontrib>XU, WENYUE</creatorcontrib><creatorcontrib>ZHOU, TAOLI</creatorcontrib><creatorcontrib>LIU, TAIPING</creatorcontrib><creatorcontrib>ZHENG, HONG</creatorcontrib><creatorcontrib>FU, YONG</creatorcontrib><title>Establishment of a murine model of cerebral malaria in KunMing mice infected with Plasmodium berghei ANKA</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>Malaria remains one of the most devastating diseases. Cerebral malaria (CM) is a severe complication of Plasmodium falciparum infection resulting in high mortality and morbidity worldwide. Analysis of precise mechanisms of CM in humans is difficult for ethical reasons and animal models of CM have been employed to study malaria pathogenesis. Here, we describe a new experimental cerebral malaria (ECM) model with Plasmodium berghei ANKA infection in KunMing (KM) mice. KM mice developed ECM after blood-stage or sporozoites infection, and the development of ECM in KM mice has a dose-dependent relationship with sporozoites inoculums. Histopathological findings revealed important features associated with ECM, including accumulation of mononuclear cells and red blood cells in brain microvascular, and brain parenchymal haemorrhages. Blood–brain barrier (BBB) examination showed that BBB disruption was present in infected KM mice when displaying clinical signs of CM. In vivo bioluminescent imaging experiment indicated that parasitized red blood cells accumulated in most vital organs including heart, lung, spleen, kidney, liver and brain. The levels of inflammatory cytokines interferon-gamma, tumour necrosis factor-alpha, interleukin (IL)-17, IL-12, IL-6 and IL-10 were all remarkably increased in KM mice infected with P. berghei ANKA. This study indicates that P. berghei ANKA infection in KM mice can be used as ECM model to extend further research on genetic, pharmacological and vaccine studies of CM.</description><subject>Animal models</subject><subject>Animal Structures - parasitology</subject><subject>Animal Structures - pathology</subject><subject>Animals</subject><subject>Disease Models, Animal</subject><subject>Erythrocytes</subject><subject>Histocytochemistry</subject><subject>Malaria</subject><subject>Malaria, Cerebral - parasitology</subject><subject>Malaria, Cerebral - pathology</subject><subject>Mice</subject><subject>Microscopy</subject><subject>Plasmodium berghei</subject><subject>Plasmodium berghei - growth & development</subject><subject>Plasmodium berghei - pathogenicity</subject><subject>Plasmodium falciparum</subject><subject>Vector-borne diseases</subject><issn>0031-1820</issn><issn>1469-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNqNkU1v1DAQhi0EotuFH8AFWeLCJTBju45zXFUFqn6ABJwjf0x2XcVJsRMh_j3ZdkGIConTaGae9x2NXsZeILxBwPrtZwCJaASgBkBVnzxiK1S6qQxqfMxW-3W13x-x41JuAEBLLZ6yI1Gf1ApQrlg8K5N1fSy7RMPEx45bnuYcB-JpDNTvJ54yuWx7nmxvc7Q8DvxiHq7isOUpelr6jvxEgX-P045_6m1ZtHFO3FHe7ijyzfXF5hl70tm-0PNDXbOv786-nH6oLj--Pz_dXFZegZ4qITowHrvQBGltV1spTHDCITiwTgo0ymhBjQ3aCfRoglRBB22EDt4ZJdfs9b3vbR6_zVSmNsXiqe_tQONcWjTaSNnUSv8Hik0DSiyCNXv1F3ozznlYHrmjhFQgxELhPeXzWEqmrr3NMdn8o0Vo95G1DyJbNC8PzrNLFH4rfmW0APJgapPLMWzpj9v_tP0JXVieiw</recordid><startdate>201610</startdate><enddate>201610</enddate><creator>DING, YAN</creator><creator>XU, WENYUE</creator><creator>ZHOU, TAOLI</creator><creator>LIU, TAIPING</creator><creator>ZHENG, HONG</creator><creator>FU, YONG</creator><general>Cambridge University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201610</creationdate><title>Establishment of a murine model of cerebral malaria in KunMing mice infected with Plasmodium berghei ANKA</title><author>DING, YAN ; XU, WENYUE ; ZHOU, TAOLI ; LIU, TAIPING ; ZHENG, HONG ; FU, YONG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-22f08c1fd9d3aaf7a328db2b10b0ab32184862e9ad6b21c18d34d6d6826dcb843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animal models</topic><topic>Animal Structures - parasitology</topic><topic>Animal Structures - pathology</topic><topic>Animals</topic><topic>Disease Models, Animal</topic><topic>Erythrocytes</topic><topic>Histocytochemistry</topic><topic>Malaria</topic><topic>Malaria, Cerebral - parasitology</topic><topic>Malaria, Cerebral - pathology</topic><topic>Mice</topic><topic>Microscopy</topic><topic>Plasmodium berghei</topic><topic>Plasmodium berghei - growth & development</topic><topic>Plasmodium berghei - pathogenicity</topic><topic>Plasmodium falciparum</topic><topic>Vector-borne diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DING, YAN</creatorcontrib><creatorcontrib>XU, WENYUE</creatorcontrib><creatorcontrib>ZHOU, TAOLI</creatorcontrib><creatorcontrib>LIU, TAIPING</creatorcontrib><creatorcontrib>ZHENG, HONG</creatorcontrib><creatorcontrib>FU, YONG</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DING, YAN</au><au>XU, WENYUE</au><au>ZHOU, TAOLI</au><au>LIU, TAIPING</au><au>ZHENG, HONG</au><au>FU, YONG</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of a murine model of cerebral malaria in KunMing mice infected with Plasmodium berghei ANKA</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>2016-10</date><risdate>2016</risdate><volume>143</volume><issue>12</issue><spage>1672</spage><epage>1680</epage><pages>1672-1680</pages><issn>0031-1820</issn><eissn>1469-8161</eissn><abstract>Malaria remains one of the most devastating diseases. Cerebral malaria (CM) is a severe complication of Plasmodium falciparum infection resulting in high mortality and morbidity worldwide. Analysis of precise mechanisms of CM in humans is difficult for ethical reasons and animal models of CM have been employed to study malaria pathogenesis. Here, we describe a new experimental cerebral malaria (ECM) model with Plasmodium berghei ANKA infection in KunMing (KM) mice. KM mice developed ECM after blood-stage or sporozoites infection, and the development of ECM in KM mice has a dose-dependent relationship with sporozoites inoculums. Histopathological findings revealed important features associated with ECM, including accumulation of mononuclear cells and red blood cells in brain microvascular, and brain parenchymal haemorrhages. Blood–brain barrier (BBB) examination showed that BBB disruption was present in infected KM mice when displaying clinical signs of CM. In vivo bioluminescent imaging experiment indicated that parasitized red blood cells accumulated in most vital organs including heart, lung, spleen, kidney, liver and brain. The levels of inflammatory cytokines interferon-gamma, tumour necrosis factor-alpha, interleukin (IL)-17, IL-12, IL-6 and IL-10 were all remarkably increased in KM mice infected with P. berghei ANKA. This study indicates that P. berghei ANKA infection in KM mice can be used as ECM model to extend further research on genetic, pharmacological and vaccine studies of CM.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>27574013</pmid><doi>10.1017/S0031182016001475</doi><tpages>9</tpages></addata></record> |
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| subjects | Animal models Animal Structures - parasitology Animal Structures - pathology Animals Disease Models, Animal Erythrocytes Histocytochemistry Malaria Malaria, Cerebral - parasitology Malaria, Cerebral - pathology Mice Microscopy Plasmodium berghei Plasmodium berghei - growth & development Plasmodium berghei - pathogenicity Plasmodium falciparum Vector-borne diseases |
| title | Establishment of a murine model of cerebral malaria in KunMing mice infected with Plasmodium berghei ANKA |
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