The Hen’s Egg Test on Chorioallantoic Membrane: An Alternative Assay for the Assessment of the Irritating Effect of Vaccine Adjuvants
Local reactions are the most frequent adverse event associated with vaccines. Adjuvants are major constituents of many vaccines and they are frequently involved in these reactions, associated with their irritating effect and the stimulation of local inflammation. The hen’s egg test on chorioallantoi...
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| Veröffentlicht in: | International journal of toxicology 2016-11, Vol.35 (6), p.627-633 |
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| creator | Batista-Duharte, Alexander Jorge Murillo, Gisela Pérez, Ulpiano Marqués Tur, Enieyis Naranjo Portuondo, Deivys Fuentes Martínez, Bruno Téllez Téllez-Martínez, Damiana Betancourt, Juan E. Pérez, Oliver |
| description | Local reactions are the most frequent adverse event associated with vaccines. Adjuvants are major constituents of many vaccines and they are frequently involved in these reactions, associated with their irritating effect and the stimulation of local inflammation. The hen’s egg test on chorioallantoic membrane (HET-CAM) is an alternative toxicological method widely used to determine ocular irritation potential, but very few studies have demonstrated the utility of this method for assessing the irritant properties of vaccine adjuvants. In this work, known/experimental adjuvants were evaluated by both HET-CAM and an in vivo local toxicity study in mice to compare irritation scores to determine whether there was a correlation (Pearson test). Based on these data (r = 0.9034; P < 0.0001), the HET-CAM assay can be used as an alternate method for the prediction of the local toxicity potential of adjuvant candidates to be used in vaccines. |
| doi_str_mv | 10.1177/1091581816672187 |
| format | Article |
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Adjuvants are major constituents of many vaccines and they are frequently involved in these reactions, associated with their irritating effect and the stimulation of local inflammation. The hen’s egg test on chorioallantoic membrane (HET-CAM) is an alternative toxicological method widely used to determine ocular irritation potential, but very few studies have demonstrated the utility of this method for assessing the irritant properties of vaccine adjuvants. In this work, known/experimental adjuvants were evaluated by both HET-CAM and an in vivo local toxicity study in mice to compare irritation scores to determine whether there was a correlation (Pearson test). Based on these data (r = 0.9034; P < 0.0001), the HET-CAM assay can be used as an alternate method for the prediction of the local toxicity potential of adjuvant candidates to be used in vaccines.</description><identifier>ISSN: 1091-5818</identifier><identifier>EISSN: 1092-874X</identifier><identifier>DOI: 10.1177/1091581816672187</identifier><identifier>PMID: 27733445</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Adjuvants, Immunologic - toxicity ; Animal Testing Alternatives ; Animals ; Biological Assay ; Chickens ; Chorioallantoic Membrane - drug effects ; Female ; Irritants - toxicity ; Mice, Inbred BALB C ; Skin - drug effects ; Vaccines</subject><ispartof>International journal of toxicology, 2016-11, Vol.35 (6), p.627-633</ispartof><rights>The Author(s) 2016</rights><rights>The Author(s) 2016.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c323t-96eff22962d0459c9db37e2210cd15d0be0fb92b932e086d90a9003381a07d863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/1091581816672187$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/1091581816672187$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21798,27901,27902,43597,43598</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27733445$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Batista-Duharte, Alexander</creatorcontrib><creatorcontrib>Jorge Murillo, Gisela</creatorcontrib><creatorcontrib>Pérez, Ulpiano Marqués</creatorcontrib><creatorcontrib>Tur, Enieyis Naranjo</creatorcontrib><creatorcontrib>Portuondo, Deivys Fuentes</creatorcontrib><creatorcontrib>Martínez, Bruno Téllez</creatorcontrib><creatorcontrib>Téllez-Martínez, Damiana</creatorcontrib><creatorcontrib>Betancourt, Juan E.</creatorcontrib><creatorcontrib>Pérez, Oliver</creatorcontrib><title>The Hen’s Egg Test on Chorioallantoic Membrane: An Alternative Assay for the Assessment of the Irritating Effect of Vaccine Adjuvants</title><title>International journal of toxicology</title><addtitle>Int J Toxicol</addtitle><description>Local reactions are the most frequent adverse event associated with vaccines. Adjuvants are major constituents of many vaccines and they are frequently involved in these reactions, associated with their irritating effect and the stimulation of local inflammation. The hen’s egg test on chorioallantoic membrane (HET-CAM) is an alternative toxicological method widely used to determine ocular irritation potential, but very few studies have demonstrated the utility of this method for assessing the irritant properties of vaccine adjuvants. In this work, known/experimental adjuvants were evaluated by both HET-CAM and an in vivo local toxicity study in mice to compare irritation scores to determine whether there was a correlation (Pearson test). Based on these data (r = 0.9034; P < 0.0001), the HET-CAM assay can be used as an alternate method for the prediction of the local toxicity potential of adjuvant candidates to be used in vaccines.</description><subject>Adjuvants, Immunologic - toxicity</subject><subject>Animal Testing Alternatives</subject><subject>Animals</subject><subject>Biological Assay</subject><subject>Chickens</subject><subject>Chorioallantoic Membrane - drug effects</subject><subject>Female</subject><subject>Irritants - toxicity</subject><subject>Mice, Inbred BALB C</subject><subject>Skin - drug effects</subject><subject>Vaccines</subject><issn>1091-5818</issn><issn>1092-874X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kL9Ow0AMxk8IRKGwM6GMLAH7Lrk_I6oKRSpiKRJbdEmcNlWTK3fNwMZr8Ho8CSktDEhMtuyfP33-GLtAuEZU6gbBYKpRo5SKo1YH7KQf8Vir5OXwu8d4ux-w0xCWACBVisdswJUSIknSEwazBUUTaj_fP0I0ns-jGYVN5NpotHC-dna1su3G1UX0SE3ubUtn7Kiyq0Dn-zpkz3fj2WgST5_uH0a307gQXGxiI6mqODeSl5CkpjBlLhRxjlCUmJaQE1S54bkRnEDL0oA1AEJotKBKLcWQXe101969dr2prKlDQVs_5LqQoZa6xxOOPQo7tPAuBE9VtvZ1Y_1bhpBtc8r-5tSfXO7Vu7yh8vfgJ5geiHdAsHPKlq7zbf_t_4JfKz1txQ</recordid><startdate>201611</startdate><enddate>201611</enddate><creator>Batista-Duharte, Alexander</creator><creator>Jorge Murillo, Gisela</creator><creator>Pérez, Ulpiano Marqués</creator><creator>Tur, Enieyis Naranjo</creator><creator>Portuondo, Deivys Fuentes</creator><creator>Martínez, Bruno Téllez</creator><creator>Téllez-Martínez, Damiana</creator><creator>Betancourt, Juan E.</creator><creator>Pérez, Oliver</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>7U7</scope><scope>C1K</scope><scope>SOI</scope></search><sort><creationdate>201611</creationdate><title>The Hen’s Egg Test on Chorioallantoic Membrane</title><author>Batista-Duharte, Alexander ; Jorge Murillo, Gisela ; Pérez, Ulpiano Marqués ; Tur, Enieyis Naranjo ; Portuondo, Deivys Fuentes ; Martínez, Bruno Téllez ; Téllez-Martínez, Damiana ; Betancourt, Juan E. ; Pérez, Oliver</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c323t-96eff22962d0459c9db37e2210cd15d0be0fb92b932e086d90a9003381a07d863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adjuvants, Immunologic - toxicity</topic><topic>Animal Testing Alternatives</topic><topic>Animals</topic><topic>Biological Assay</topic><topic>Chickens</topic><topic>Chorioallantoic Membrane - drug effects</topic><topic>Female</topic><topic>Irritants - toxicity</topic><topic>Mice, Inbred BALB C</topic><topic>Skin - drug effects</topic><topic>Vaccines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Batista-Duharte, Alexander</creatorcontrib><creatorcontrib>Jorge Murillo, Gisela</creatorcontrib><creatorcontrib>Pérez, Ulpiano Marqués</creatorcontrib><creatorcontrib>Tur, Enieyis Naranjo</creatorcontrib><creatorcontrib>Portuondo, Deivys Fuentes</creatorcontrib><creatorcontrib>Martínez, Bruno Téllez</creatorcontrib><creatorcontrib>Téllez-Martínez, Damiana</creatorcontrib><creatorcontrib>Betancourt, Juan E.</creatorcontrib><creatorcontrib>Pérez, Oliver</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><jtitle>International journal of toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Batista-Duharte, Alexander</au><au>Jorge Murillo, Gisela</au><au>Pérez, Ulpiano Marqués</au><au>Tur, Enieyis Naranjo</au><au>Portuondo, Deivys Fuentes</au><au>Martínez, Bruno Téllez</au><au>Téllez-Martínez, Damiana</au><au>Betancourt, Juan E.</au><au>Pérez, Oliver</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Hen’s Egg Test on Chorioallantoic Membrane: An Alternative Assay for the Assessment of the Irritating Effect of Vaccine Adjuvants</atitle><jtitle>International journal of toxicology</jtitle><addtitle>Int J Toxicol</addtitle><date>2016-11</date><risdate>2016</risdate><volume>35</volume><issue>6</issue><spage>627</spage><epage>633</epage><pages>627-633</pages><issn>1091-5818</issn><eissn>1092-874X</eissn><abstract>Local reactions are the most frequent adverse event associated with vaccines. 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| ispartof | International journal of toxicology, 2016-11, Vol.35 (6), p.627-633 |
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| subjects | Adjuvants, Immunologic - toxicity Animal Testing Alternatives Animals Biological Assay Chickens Chorioallantoic Membrane - drug effects Female Irritants - toxicity Mice, Inbred BALB C Skin - drug effects Vaccines |
| title | The Hen’s Egg Test on Chorioallantoic Membrane: An Alternative Assay for the Assessment of the Irritating Effect of Vaccine Adjuvants |
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