A rapid, highly sensitive and culture-free detection of pathogens from blood by positive enrichment

Molecular diagnostics is a promising alternative to culture based methods for the detection of bloodstream infections, notably due to its overall lower turnaround time when starting directly from patient samples. Whole blood is usually the starting diagnostic sample in suspected bloodstream infectio...

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Veröffentlicht in:	Journal of microbiological methods 2016-12, Vol.131, p.105-109
Hauptverfasser:	Vutukuru, Manjula Ramya, Sharma, Divya Khandige, Ragavendar, MS, Schmolke, Susanne, Huang, Yiwei, Gumbrecht, Walter, Mitra, Nivedita
Format:	Artikel
Sprache:	eng
Schlagworte:	Apolipoprotein H Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Bacteria - pathogenicity beta 2-Glycoprotein I - administration & dosage Blood - microbiology Blood stream infections Blood-Borne Pathogens - isolation & purification Candida tropicalis Candida tropicalis - genetics Candida tropicalis - isolation & purification Cells, Cultured Colony Count, Microbial - methods Culture-free detection DNA, Bacterial - blood DNA, Fungal - blood Enterococcus - genetics Enterococcus - isolation & purification Enterococcus gallinarum Escherichia coli Escherichia coli - genetics Escherichia coli - isolation & purification Fungi - classification Fungi - genetics Fungi - isolation & purification Fungi - pathogenicity Genome, Human Humans Limit of Detection Microbiological Techniques - methods Pathogen enrichment Pathology, Molecular - methods Quantitative PCR Real-Time Polymerase Chain Reaction - methods Sensitivity and Specificity Sepsis Sepsis - blood Sepsis - diagnosis Sepsis - microbiology Time Factors
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