Genotyping of German and Austrian Taylorella equigenitalis isolates using repetitive extragenic palindromic (REP) PCR and pulsed-field gel electrophoresis (PFGE)
A total of 124 Taylorella (T.) equigenitalis and five T. asinigenitalis field isolates collected between 2002 and 2014 were available for genotyping using REP- (repetitive extragenic palindromic) PCR and PFGE (pulsed-field gel electrophoresis). The study comprised 79 T. equigenitalis field isolates...
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| Veröffentlicht in: | Research in veterinary science 2016-12, Vol.109, p.101-106 |
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| creator | Sting, Reinhard Seeh, Christoph Mauder, Norman Maurer, Michaela Loncaric, Igor Stessl, Beatrix Kopp, Peter Banzhaf, Klaus Martin, Brigitte Melzer, Falk Raßbach, Astrid Spergser, Joachim |
| description | A total of 124 Taylorella (T.) equigenitalis and five T. asinigenitalis field isolates collected between 2002 and 2014 were available for genotyping using REP- (repetitive extragenic palindromic) PCR and PFGE (pulsed-field gel electrophoresis). The study comprised 79 T. equigenitalis field isolates originating from ten defined breeds of German horses and revealed a spectrum of five REP (rep-E1-E4, rep-E3a) and 15 PFGE (TE-A1-A9, TE-B1-B3, TE-C, TE-E1, and TE-E2) genotypes. T. equigenitalis field isolates (n=40) obtained from Austrian Lipizzaner horses were differentiated into three REP (rep-E1, rep-E3a, and rep-E4) and three PFGE genotypes (TE-A2, TE-A5, and TE-D); those isolated from four Austrian Trotters belonged to the REP/PFGE genotype rep-E2/TE-A1. Interestingly, a T. equigenitalis isolate recovered from a Holsteiner stallion living in South Africa revealed the REP/PFGE genotype rep-E1/TE-A5 which was otherwise exclusively present in the majority of Austrian Lipizzaner horses in our study. The type strain included in this study revealed the genotype REP/PFGE rep-E1/TE-F. Six strains of T. asinigenitalis including the type strain were separated into three REP (rep-A1-A3) and six PFGE genotypes (TA-A1, TA-A2, TA-A3, TA-B, TA-C, TA-D). Overall, the generated REP and PFGE genotypes showed a good correlation, whereas REP-PCR proved to be a suitable method for molecular epidemiological screening of T. equigenitalis and T. asinigenitalis isolates that should be differentiated in detail by genotyping using PFGE. |
| doi_str_mv | 10.1016/j.rvsc.2016.09.017 |
| format | Article |
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The study comprised 79 T. equigenitalis field isolates originating from ten defined breeds of German horses and revealed a spectrum of five REP (rep-E1-E4, rep-E3a) and 15 PFGE (TE-A1-A9, TE-B1-B3, TE-C, TE-E1, and TE-E2) genotypes. T. equigenitalis field isolates (n=40) obtained from Austrian Lipizzaner horses were differentiated into three REP (rep-E1, rep-E3a, and rep-E4) and three PFGE genotypes (TE-A2, TE-A5, and TE-D); those isolated from four Austrian Trotters belonged to the REP/PFGE genotype rep-E2/TE-A1. Interestingly, a T. equigenitalis isolate recovered from a Holsteiner stallion living in South Africa revealed the REP/PFGE genotype rep-E1/TE-A5 which was otherwise exclusively present in the majority of Austrian Lipizzaner horses in our study. The type strain included in this study revealed the genotype REP/PFGE rep-E1/TE-F. Six strains of T. asinigenitalis including the type strain were separated into three REP (rep-A1-A3) and six PFGE genotypes (TA-A1, TA-A2, TA-A3, TA-B, TA-C, TA-D). Overall, the generated REP and PFGE genotypes showed a good correlation, whereas REP-PCR proved to be a suitable method for molecular epidemiological screening of T. equigenitalis and T. asinigenitalis isolates that should be differentiated in detail by genotyping using PFGE.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/j.rvsc.2016.09.017</identifier><identifier>PMID: 27892857</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Austria ; Electrophoresis, Gel, Pulsed-Field - veterinary ; Epidemics ; Female ; Genotype ; Genotype & phenotype ; Genotyping ; Germany ; Gram-Negative Bacterial Infections - genetics ; Gram-Negative Bacterial Infections - microbiology ; Gram-Negative Bacterial Infections - veterinary ; Horse Diseases - genetics ; Horse Diseases - microbiology ; Horses ; Inverted Repeat Sequences ; Male ; PFGE ; Polymerase Chain Reaction - veterinary ; Rep-PCR ; Taylorella asinigenitalis ; Taylorella equigenitalis ; Taylorella equigenitalis - genetics ; Veterinary medicine</subject><ispartof>Research in veterinary science, 2016-12, Vol.109, p.101-106</ispartof><rights>2016 Elsevier Ltd</rights><rights>Copyright © 2016 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Limited Dec 01, 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-fcf9dea34cab4933ff5ac1ed4d9197a9588de3bac604204299cf2de070015c893</citedby><cites>FETCH-LOGICAL-c417t-fcf9dea34cab4933ff5ac1ed4d9197a9588de3bac604204299cf2de070015c893</cites><orcidid>0000-0002-1437-5036</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.rvsc.2016.09.017$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27892857$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sting, Reinhard</creatorcontrib><creatorcontrib>Seeh, Christoph</creatorcontrib><creatorcontrib>Mauder, Norman</creatorcontrib><creatorcontrib>Maurer, Michaela</creatorcontrib><creatorcontrib>Loncaric, Igor</creatorcontrib><creatorcontrib>Stessl, Beatrix</creatorcontrib><creatorcontrib>Kopp, Peter</creatorcontrib><creatorcontrib>Banzhaf, Klaus</creatorcontrib><creatorcontrib>Martin, Brigitte</creatorcontrib><creatorcontrib>Melzer, Falk</creatorcontrib><creatorcontrib>Raßbach, Astrid</creatorcontrib><creatorcontrib>Spergser, Joachim</creatorcontrib><title>Genotyping of German and Austrian Taylorella equigenitalis isolates using repetitive extragenic palindromic (REP) PCR and pulsed-field gel electrophoresis (PFGE)</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>A total of 124 Taylorella (T.) equigenitalis and five T. asinigenitalis field isolates collected between 2002 and 2014 were available for genotyping using REP- (repetitive extragenic palindromic) PCR and PFGE (pulsed-field gel electrophoresis). The study comprised 79 T. equigenitalis field isolates originating from ten defined breeds of German horses and revealed a spectrum of five REP (rep-E1-E4, rep-E3a) and 15 PFGE (TE-A1-A9, TE-B1-B3, TE-C, TE-E1, and TE-E2) genotypes. T. equigenitalis field isolates (n=40) obtained from Austrian Lipizzaner horses were differentiated into three REP (rep-E1, rep-E3a, and rep-E4) and three PFGE genotypes (TE-A2, TE-A5, and TE-D); those isolated from four Austrian Trotters belonged to the REP/PFGE genotype rep-E2/TE-A1. Interestingly, a T. equigenitalis isolate recovered from a Holsteiner stallion living in South Africa revealed the REP/PFGE genotype rep-E1/TE-A5 which was otherwise exclusively present in the majority of Austrian Lipizzaner horses in our study. The type strain included in this study revealed the genotype REP/PFGE rep-E1/TE-F. Six strains of T. asinigenitalis including the type strain were separated into three REP (rep-A1-A3) and six PFGE genotypes (TA-A1, TA-A2, TA-A3, TA-B, TA-C, TA-D). 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The study comprised 79 T. equigenitalis field isolates originating from ten defined breeds of German horses and revealed a spectrum of five REP (rep-E1-E4, rep-E3a) and 15 PFGE (TE-A1-A9, TE-B1-B3, TE-C, TE-E1, and TE-E2) genotypes. T. equigenitalis field isolates (n=40) obtained from Austrian Lipizzaner horses were differentiated into three REP (rep-E1, rep-E3a, and rep-E4) and three PFGE genotypes (TE-A2, TE-A5, and TE-D); those isolated from four Austrian Trotters belonged to the REP/PFGE genotype rep-E2/TE-A1. Interestingly, a T. equigenitalis isolate recovered from a Holsteiner stallion living in South Africa revealed the REP/PFGE genotype rep-E1/TE-A5 which was otherwise exclusively present in the majority of Austrian Lipizzaner horses in our study. The type strain included in this study revealed the genotype REP/PFGE rep-E1/TE-F. Six strains of T. asinigenitalis including the type strain were separated into three REP (rep-A1-A3) and six PFGE genotypes (TA-A1, TA-A2, TA-A3, TA-B, TA-C, TA-D). Overall, the generated REP and PFGE genotypes showed a good correlation, whereas REP-PCR proved to be a suitable method for molecular epidemiological screening of T. equigenitalis and T. asinigenitalis isolates that should be differentiated in detail by genotyping using PFGE.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>27892857</pmid><doi>10.1016/j.rvsc.2016.09.017</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-1437-5036</orcidid></addata></record> |
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| ispartof | Research in veterinary science, 2016-12, Vol.109, p.101-106 |
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| subjects | Animals Austria Electrophoresis, Gel, Pulsed-Field - veterinary Epidemics Female Genotype Genotype & phenotype Genotyping Germany Gram-Negative Bacterial Infections - genetics Gram-Negative Bacterial Infections - microbiology Gram-Negative Bacterial Infections - veterinary Horse Diseases - genetics Horse Diseases - microbiology Horses Inverted Repeat Sequences Male PFGE Polymerase Chain Reaction - veterinary Rep-PCR Taylorella asinigenitalis Taylorella equigenitalis Taylorella equigenitalis - genetics Veterinary medicine |
| title | Genotyping of German and Austrian Taylorella equigenitalis isolates using repetitive extragenic palindromic (REP) PCR and pulsed-field gel electrophoresis (PFGE) |
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