Presence of Clostridium difficile in pig faecal samples and wild animal species associated with pig farms
Aims To determine the presence of Clostridium difficile on fattening pig farms in north‐eastern Spain. Methods and Results Twenty‐seven farms were sampled. Pools of pig faecal samples (n = 210), samples of intestinal content from common farm pest species (n = 95) and environment‐related samples (n =...
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| Veröffentlicht in: | Journal of applied microbiology 2017-02, Vol.122 (2), p.462-472 |
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| container_title | Journal of applied microbiology |
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| creator | Andrés‐Lasheras, S. Bolea, R. Mainar‐Jaime, R.C. Kuijper, E. Sevilla, E. Martín‐Burriel, I. Chirino‐Trejo, M. |
| description | Aims
To determine the presence of Clostridium difficile on fattening pig farms in north‐eastern Spain.
Methods and Results
Twenty‐seven farms were sampled. Pools of pig faecal samples (n = 210), samples of intestinal content from common farm pest species (n = 95) and environment‐related samples (n = 93) were collected. Isolates were tested for toxin genes of C. difficile, and typed by PCR‐ribotyping and toxinotyping. The minimal inhibitory concentrations of six antimicrobial agents were determined using Etest. Thirty‐four isolates were obtained from 12 farms, and 30 (88·2%) had toxin genes. Seven ribotypes were identified. Ribotype 078 and its variant 126 were predominant (52·9%). The same ribotypes were isolated from different animal species on the same farm. None of the isolates were resistant to metronidazole or vancomycin.
Conclusions
Clostridium difficile was common within the pig farm environment. Most of the positive samples came from pest species or were pest‐related environmental samples.
Significance and Impact of the Study
Pest species were colonized with toxigenic and antimicrobial‐resistant C. difficile strains of the same ribotypes that are found in humans and pigs. Rodents and pigeons may transmit toxigenic and antimicrobial‐resistant C. difficile strains that are of the same ribotypes as those occuring in humans. |
| doi_str_mv | 10.1111/jam.13343 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1868326787</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1868326787</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3863-c6f5327b5f7f51867fdf5a5d1092aaeb62783620bbfe702982e55c7c5a22059c3</originalsourceid><addsrcrecordid>eNp1kM1PwyAchonRuDk9-A8YEi966EZhQHtcFj8zowc9N5T-UJZ-CW3M_nvpNj2YyIVfXh7ewIPQeUymcViztaqmMWNzdoDGMRM8okLSw-08jziRdIROvF8TEjPCxTEaUZmmIWZjZF8ceKg14MbgZdn4ztnC9hUurDFW2xKwrXFr37FRoFWJvaraEjxWdYG_bFmEwVZD3oK2Q-59o63qYDjuPvZXXeVP0ZFRpYez_T5Bb7c3r8v7aPV897BcrCLNEsEiLQxnVObcSMPjREhTGK54EZOUKgW5oDJhgpI8NyAJTRMKnGupuaKU8FSzCbra9bau-ezBd1llvYayVDU0vc9CZ8KCoEQG9PIPum56V4fXBYrLVAjGRaCud5R2jfcOTNa68GW3yWKSDf6z4D_b-g_sxb6xzysofskf4QGY7YDgDjb_N2WPi6dd5Tf7II5v</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1857966356</pqid></control><display><type>article</type><title>Presence of Clostridium difficile in pig faecal samples and wild animal species associated with pig farms</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Andrés‐Lasheras, S. ; Bolea, R. ; Mainar‐Jaime, R.C. ; Kuijper, E. ; Sevilla, E. ; Martín‐Burriel, I. ; Chirino‐Trejo, M.</creator><creatorcontrib>Andrés‐Lasheras, S. ; Bolea, R. ; Mainar‐Jaime, R.C. ; Kuijper, E. ; Sevilla, E. ; Martín‐Burriel, I. ; Chirino‐Trejo, M.</creatorcontrib><description>Aims
To determine the presence of Clostridium difficile on fattening pig farms in north‐eastern Spain.
Methods and Results
Twenty‐seven farms were sampled. Pools of pig faecal samples (n = 210), samples of intestinal content from common farm pest species (n = 95) and environment‐related samples (n = 93) were collected. Isolates were tested for toxin genes of C. difficile, and typed by PCR‐ribotyping and toxinotyping. The minimal inhibitory concentrations of six antimicrobial agents were determined using Etest. Thirty‐four isolates were obtained from 12 farms, and 30 (88·2%) had toxin genes. Seven ribotypes were identified. Ribotype 078 and its variant 126 were predominant (52·9%). The same ribotypes were isolated from different animal species on the same farm. None of the isolates were resistant to metronidazole or vancomycin.
Conclusions
Clostridium difficile was common within the pig farm environment. Most of the positive samples came from pest species or were pest‐related environmental samples.
Significance and Impact of the Study
Pest species were colonized with toxigenic and antimicrobial‐resistant C. difficile strains of the same ribotypes that are found in humans and pigs. Rodents and pigeons may transmit toxigenic and antimicrobial‐resistant C. difficile strains that are of the same ribotypes as those occuring in humans.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.13343</identifier><identifier>PMID: 27990723</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Animals ; Animals, Wild ; Anti-Bacterial Agents - pharmacology ; Bacteriology ; Clostridium difficile ; Clostridium difficile - classification ; Clostridium difficile - isolation & purification ; Clostridium Infections - transmission ; Clostridium Infections - veterinary ; environment ; Farms ; Feces ; Feces - microbiology ; Hogs ; Humans ; Metronidazole - pharmacology ; Microbial Sensitivity Tests ; Microbiology ; PCR‐ribotyping ; pig ; Polymerase Chain Reaction ; Ribotyping ; rodents ; Spain ; Sus scrofa ; toxins ; wildlife</subject><ispartof>Journal of applied microbiology, 2017-02, Vol.122 (2), p.462-472</ispartof><rights>2016 The Society for Applied Microbiology</rights><rights>2016 The Society for Applied Microbiology.</rights><rights>Copyright © 2017 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3863-c6f5327b5f7f51867fdf5a5d1092aaeb62783620bbfe702982e55c7c5a22059c3</citedby><cites>FETCH-LOGICAL-c3863-c6f5327b5f7f51867fdf5a5d1092aaeb62783620bbfe702982e55c7c5a22059c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.13343$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.13343$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27990723$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Andrés‐Lasheras, S.</creatorcontrib><creatorcontrib>Bolea, R.</creatorcontrib><creatorcontrib>Mainar‐Jaime, R.C.</creatorcontrib><creatorcontrib>Kuijper, E.</creatorcontrib><creatorcontrib>Sevilla, E.</creatorcontrib><creatorcontrib>Martín‐Burriel, I.</creatorcontrib><creatorcontrib>Chirino‐Trejo, M.</creatorcontrib><title>Presence of Clostridium difficile in pig faecal samples and wild animal species associated with pig farms</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
To determine the presence of Clostridium difficile on fattening pig farms in north‐eastern Spain.
Methods and Results
Twenty‐seven farms were sampled. Pools of pig faecal samples (n = 210), samples of intestinal content from common farm pest species (n = 95) and environment‐related samples (n = 93) were collected. Isolates were tested for toxin genes of C. difficile, and typed by PCR‐ribotyping and toxinotyping. The minimal inhibitory concentrations of six antimicrobial agents were determined using Etest. Thirty‐four isolates were obtained from 12 farms, and 30 (88·2%) had toxin genes. Seven ribotypes were identified. Ribotype 078 and its variant 126 were predominant (52·9%). The same ribotypes were isolated from different animal species on the same farm. None of the isolates were resistant to metronidazole or vancomycin.
Conclusions
Clostridium difficile was common within the pig farm environment. Most of the positive samples came from pest species or were pest‐related environmental samples.
Significance and Impact of the Study
Pest species were colonized with toxigenic and antimicrobial‐resistant C. difficile strains of the same ribotypes that are found in humans and pigs. Rodents and pigeons may transmit toxigenic and antimicrobial‐resistant C. difficile strains that are of the same ribotypes as those occuring in humans.</description><subject>Animals</subject><subject>Animals, Wild</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bacteriology</subject><subject>Clostridium difficile</subject><subject>Clostridium difficile - classification</subject><subject>Clostridium difficile - isolation & purification</subject><subject>Clostridium Infections - transmission</subject><subject>Clostridium Infections - veterinary</subject><subject>environment</subject><subject>Farms</subject><subject>Feces</subject><subject>Feces - microbiology</subject><subject>Hogs</subject><subject>Humans</subject><subject>Metronidazole - pharmacology</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>PCR‐ribotyping</subject><subject>pig</subject><subject>Polymerase Chain Reaction</subject><subject>Ribotyping</subject><subject>rodents</subject><subject>Spain</subject><subject>Sus scrofa</subject><subject>toxins</subject><subject>wildlife</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1PwyAchonRuDk9-A8YEi966EZhQHtcFj8zowc9N5T-UJZ-CW3M_nvpNj2YyIVfXh7ewIPQeUymcViztaqmMWNzdoDGMRM8okLSw-08jziRdIROvF8TEjPCxTEaUZmmIWZjZF8ceKg14MbgZdn4ztnC9hUurDFW2xKwrXFr37FRoFWJvaraEjxWdYG_bFmEwVZD3oK2Q-59o63qYDjuPvZXXeVP0ZFRpYez_T5Bb7c3r8v7aPV897BcrCLNEsEiLQxnVObcSMPjREhTGK54EZOUKgW5oDJhgpI8NyAJTRMKnGupuaKU8FSzCbra9bau-ezBd1llvYayVDU0vc9CZ8KCoEQG9PIPum56V4fXBYrLVAjGRaCud5R2jfcOTNa68GW3yWKSDf6z4D_b-g_sxb6xzysofskf4QGY7YDgDjb_N2WPi6dd5Tf7II5v</recordid><startdate>201702</startdate><enddate>201702</enddate><creator>Andrés‐Lasheras, S.</creator><creator>Bolea, R.</creator><creator>Mainar‐Jaime, R.C.</creator><creator>Kuijper, E.</creator><creator>Sevilla, E.</creator><creator>Martín‐Burriel, I.</creator><creator>Chirino‐Trejo, M.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>201702</creationdate><title>Presence of Clostridium difficile in pig faecal samples and wild animal species associated with pig farms</title><author>Andrés‐Lasheras, S. ; Bolea, R. ; Mainar‐Jaime, R.C. ; Kuijper, E. ; Sevilla, E. ; Martín‐Burriel, I. ; Chirino‐Trejo, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3863-c6f5327b5f7f51867fdf5a5d1092aaeb62783620bbfe702982e55c7c5a22059c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Animals, Wild</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Bacteriology</topic><topic>Clostridium difficile</topic><topic>Clostridium difficile - classification</topic><topic>Clostridium difficile - isolation & purification</topic><topic>Clostridium Infections - transmission</topic><topic>Clostridium Infections - veterinary</topic><topic>environment</topic><topic>Farms</topic><topic>Feces</topic><topic>Feces - microbiology</topic><topic>Hogs</topic><topic>Humans</topic><topic>Metronidazole - pharmacology</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>PCR‐ribotyping</topic><topic>pig</topic><topic>Polymerase Chain Reaction</topic><topic>Ribotyping</topic><topic>rodents</topic><topic>Spain</topic><topic>Sus scrofa</topic><topic>toxins</topic><topic>wildlife</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Andrés‐Lasheras, S.</creatorcontrib><creatorcontrib>Bolea, R.</creatorcontrib><creatorcontrib>Mainar‐Jaime, R.C.</creatorcontrib><creatorcontrib>Kuijper, E.</creatorcontrib><creatorcontrib>Sevilla, E.</creatorcontrib><creatorcontrib>Martín‐Burriel, I.</creatorcontrib><creatorcontrib>Chirino‐Trejo, M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Andrés‐Lasheras, S.</au><au>Bolea, R.</au><au>Mainar‐Jaime, R.C.</au><au>Kuijper, E.</au><au>Sevilla, E.</au><au>Martín‐Burriel, I.</au><au>Chirino‐Trejo, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Presence of Clostridium difficile in pig faecal samples and wild animal species associated with pig farms</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2017-02</date><risdate>2017</risdate><volume>122</volume><issue>2</issue><spage>462</spage><epage>472</epage><pages>462-472</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>Aims
To determine the presence of Clostridium difficile on fattening pig farms in north‐eastern Spain.
Methods and Results
Twenty‐seven farms were sampled. Pools of pig faecal samples (n = 210), samples of intestinal content from common farm pest species (n = 95) and environment‐related samples (n = 93) were collected. Isolates were tested for toxin genes of C. difficile, and typed by PCR‐ribotyping and toxinotyping. The minimal inhibitory concentrations of six antimicrobial agents were determined using Etest. Thirty‐four isolates were obtained from 12 farms, and 30 (88·2%) had toxin genes. Seven ribotypes were identified. Ribotype 078 and its variant 126 were predominant (52·9%). The same ribotypes were isolated from different animal species on the same farm. None of the isolates were resistant to metronidazole or vancomycin.
Conclusions
Clostridium difficile was common within the pig farm environment. Most of the positive samples came from pest species or were pest‐related environmental samples.
Significance and Impact of the Study
Pest species were colonized with toxigenic and antimicrobial‐resistant C. difficile strains of the same ribotypes that are found in humans and pigs. Rodents and pigeons may transmit toxigenic and antimicrobial‐resistant C. difficile strains that are of the same ribotypes as those occuring in humans.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>27990723</pmid><doi>10.1111/jam.13343</doi><tpages>11</tpages></addata></record> |
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| identifier | ISSN: 1364-5072 |
| ispartof | Journal of applied microbiology, 2017-02, Vol.122 (2), p.462-472 |
| issn | 1364-5072 1365-2672 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1868326787 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Animals, Wild Anti-Bacterial Agents - pharmacology Bacteriology Clostridium difficile Clostridium difficile - classification Clostridium difficile - isolation & purification Clostridium Infections - transmission Clostridium Infections - veterinary environment Farms Feces Feces - microbiology Hogs Humans Metronidazole - pharmacology Microbial Sensitivity Tests Microbiology PCR‐ribotyping pig Polymerase Chain Reaction Ribotyping rodents Spain Sus scrofa toxins wildlife |
| title | Presence of Clostridium difficile in pig faecal samples and wild animal species associated with pig farms |
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