Acanthamoeba genotypes T2, T4, and T11 in soil sources from El Hierro island, Canary Islands, Spain
The genus Acanthamoeba includes pathogenic strains which are causative agents of keratitis and encephalitis that often may end fatal in humans and other animals. In the present study, forty soil samples were collected in the island of El Hierro, Canary Islands, Spain, and checked for the presence of...
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| Veröffentlicht in: | Parasitology research (1987) 2016-08, Vol.115 (8), p.2953-2956 |
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| creator | Reyes-Batlle, María Zamora-Herrera, Jonadab Vargas-Mesa, Alejandro Valerón-Tejera, Marco Antonio Wagner, Carolina Martín-Navarro, Carmen Ma López-Arencibia, Atteneri Sifaoui, Ines Martínez-Carretero, Enrique Valladares, Basilio Piñero, José E. Lorenzo-Morales, Jacob |
| description | The genus
Acanthamoeba
includes pathogenic strains which are causative agents of keratitis and encephalitis that often may end fatal in humans and other animals. In the present study, forty soil samples were collected in the island of El Hierro, Canary Islands, Spain, and checked for the presence of
Acanthamoeba
. Samples were cultivated onto 2 % non-nutrient agar plates seeded with a layer of heat killed
Escherichia coli
. Amplification by PCR and sequencing of the DF3 region of the 18S rDNA of
Acanthamoeba
was carried out in order to confirm morphological identification of the amoebae. Furthermore,
Acanthamoeba
spp. was isolated from 47.5 % of soil samples. Moreover, genotypes T2, T4, and T11 were identified in these samples. To the best of our knowledge, this is the first study to establish genotypes T2, T4, and T11 in soil sources from El Hierro island. |
| doi_str_mv | 10.1007/s00436-016-5048-8 |
| format | Article |
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Acanthamoeba
includes pathogenic strains which are causative agents of keratitis and encephalitis that often may end fatal in humans and other animals. In the present study, forty soil samples were collected in the island of El Hierro, Canary Islands, Spain, and checked for the presence of
Acanthamoeba
. Samples were cultivated onto 2 % non-nutrient agar plates seeded with a layer of heat killed
Escherichia coli
. Amplification by PCR and sequencing of the DF3 region of the 18S rDNA of
Acanthamoeba
was carried out in order to confirm morphological identification of the amoebae. Furthermore,
Acanthamoeba
spp. was isolated from 47.5 % of soil samples. Moreover, genotypes T2, T4, and T11 were identified in these samples. To the best of our knowledge, this is the first study to establish genotypes T2, T4, and T11 in soil sources from El Hierro island.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-016-5048-8</identifier><identifier>PMID: 27075307</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acanthamoeba ; Acanthamoeba - genetics ; Acanthamoeba - isolation & purification ; Amoeba - genetics ; Animals ; Biomedical and Life Sciences ; Biomedicine ; DNA, Protozoan ; DNA, Ribosomal - genetics ; Escherichia coli ; Genetic aspects ; Genotype ; Genotypes ; Humans ; Immunology ; Medical Microbiology ; Microbiology ; Observations ; Original Paper ; Polymerase Chain Reaction ; Protozoans ; Soil - parasitology ; Spain</subject><ispartof>Parasitology research (1987), 2016-08, Vol.115 (8), p.2953-2956</ispartof><rights>Springer-Verlag Berlin Heidelberg 2016</rights><rights>COPYRIGHT 2016 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-85f70db96635293965aa759a7ab5b890724e85790aa3def2735a20477075eaf93</citedby><cites>FETCH-LOGICAL-c416t-85f70db96635293965aa759a7ab5b890724e85790aa3def2735a20477075eaf93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00436-016-5048-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00436-016-5048-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27075307$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reyes-Batlle, María</creatorcontrib><creatorcontrib>Zamora-Herrera, Jonadab</creatorcontrib><creatorcontrib>Vargas-Mesa, Alejandro</creatorcontrib><creatorcontrib>Valerón-Tejera, Marco Antonio</creatorcontrib><creatorcontrib>Wagner, Carolina</creatorcontrib><creatorcontrib>Martín-Navarro, Carmen Ma</creatorcontrib><creatorcontrib>López-Arencibia, Atteneri</creatorcontrib><creatorcontrib>Sifaoui, Ines</creatorcontrib><creatorcontrib>Martínez-Carretero, Enrique</creatorcontrib><creatorcontrib>Valladares, Basilio</creatorcontrib><creatorcontrib>Piñero, José E.</creatorcontrib><creatorcontrib>Lorenzo-Morales, Jacob</creatorcontrib><title>Acanthamoeba genotypes T2, T4, and T11 in soil sources from El Hierro island, Canary Islands, Spain</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>The genus
Acanthamoeba
includes pathogenic strains which are causative agents of keratitis and encephalitis that often may end fatal in humans and other animals. In the present study, forty soil samples were collected in the island of El Hierro, Canary Islands, Spain, and checked for the presence of
Acanthamoeba
. Samples were cultivated onto 2 % non-nutrient agar plates seeded with a layer of heat killed
Escherichia coli
. Amplification by PCR and sequencing of the DF3 region of the 18S rDNA of
Acanthamoeba
was carried out in order to confirm morphological identification of the amoebae. Furthermore,
Acanthamoeba
spp. was isolated from 47.5 % of soil samples. Moreover, genotypes T2, T4, and T11 were identified in these samples. To the best of our knowledge, this is the first study to establish genotypes T2, T4, and T11 in soil sources from El Hierro island.</description><subject>Acanthamoeba</subject><subject>Acanthamoeba - genetics</subject><subject>Acanthamoeba - isolation & purification</subject><subject>Amoeba - genetics</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>DNA, Protozoan</subject><subject>DNA, Ribosomal - genetics</subject><subject>Escherichia coli</subject><subject>Genetic aspects</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Humans</subject><subject>Immunology</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Observations</subject><subject>Original Paper</subject><subject>Polymerase Chain Reaction</subject><subject>Protozoans</subject><subject>Soil - parasitology</subject><subject>Spain</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EokvhB3BBPnLYlPF3fFytCq1UiQPL2ZokzuIqsRc7e-i_x0sKR4Qs2WPP847G8xLynsENAzCfCoAUugGmGwWybdoXZMOk4A2zSr0kG7A1BsbEFXlTyiMAM1rK1-SKGzBKgNmQftdjXH7gnHyH9OhjWp5OvtAD39KD3FKMAz0wRkOkJYWpbufc1_yY00xvJ3oXfM6JhjJVckv3GDE_0fvf17Kl304Y4lvyasSp-HfP5zX5_vn2sL9rHr5-ud_vHppeMr00rRoNDJ3VWihuhdUK0SiLBjvVtRYMl75VxgKiGPzIjVDIQZrLXzyOVlyTj2vdU04_z74sbg6l91PtxadzcazVreBacfEfKGgtQWio6M2KHnHyLsQxLRn7ugY_hz5FP4b6vpMGVFWpSxtsFfQ5lZL96E45zHUsjoG7-OZW31z1zV18c23VfHju59zNfvir-GNUBfgKlJqKR5_dY3Ui1nH-o-ovqQ2evA</recordid><startdate>20160801</startdate><enddate>20160801</enddate><creator>Reyes-Batlle, María</creator><creator>Zamora-Herrera, Jonadab</creator><creator>Vargas-Mesa, Alejandro</creator><creator>Valerón-Tejera, Marco Antonio</creator><creator>Wagner, Carolina</creator><creator>Martín-Navarro, Carmen Ma</creator><creator>López-Arencibia, Atteneri</creator><creator>Sifaoui, Ines</creator><creator>Martínez-Carretero, Enrique</creator><creator>Valladares, Basilio</creator><creator>Piñero, José E.</creator><creator>Lorenzo-Morales, Jacob</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>M7N</scope></search><sort><creationdate>20160801</creationdate><title>Acanthamoeba genotypes T2, T4, and T11 in soil sources from El Hierro island, Canary Islands, Spain</title><author>Reyes-Batlle, María ; Zamora-Herrera, Jonadab ; Vargas-Mesa, Alejandro ; Valerón-Tejera, Marco Antonio ; Wagner, Carolina ; Martín-Navarro, Carmen Ma ; López-Arencibia, Atteneri ; Sifaoui, Ines ; Martínez-Carretero, Enrique ; Valladares, Basilio ; Piñero, José E. ; Lorenzo-Morales, Jacob</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-85f70db96635293965aa759a7ab5b890724e85790aa3def2735a20477075eaf93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Acanthamoeba</topic><topic>Acanthamoeba - genetics</topic><topic>Acanthamoeba - isolation & purification</topic><topic>Amoeba - genetics</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>DNA, Protozoan</topic><topic>DNA, Ribosomal - genetics</topic><topic>Escherichia coli</topic><topic>Genetic aspects</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Humans</topic><topic>Immunology</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Observations</topic><topic>Original Paper</topic><topic>Polymerase Chain Reaction</topic><topic>Protozoans</topic><topic>Soil - parasitology</topic><topic>Spain</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reyes-Batlle, María</creatorcontrib><creatorcontrib>Zamora-Herrera, Jonadab</creatorcontrib><creatorcontrib>Vargas-Mesa, Alejandro</creatorcontrib><creatorcontrib>Valerón-Tejera, Marco Antonio</creatorcontrib><creatorcontrib>Wagner, Carolina</creatorcontrib><creatorcontrib>Martín-Navarro, Carmen Ma</creatorcontrib><creatorcontrib>López-Arencibia, Atteneri</creatorcontrib><creatorcontrib>Sifaoui, Ines</creatorcontrib><creatorcontrib>Martínez-Carretero, Enrique</creatorcontrib><creatorcontrib>Valladares, Basilio</creatorcontrib><creatorcontrib>Piñero, José E.</creatorcontrib><creatorcontrib>Lorenzo-Morales, Jacob</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reyes-Batlle, María</au><au>Zamora-Herrera, Jonadab</au><au>Vargas-Mesa, Alejandro</au><au>Valerón-Tejera, Marco Antonio</au><au>Wagner, Carolina</au><au>Martín-Navarro, Carmen Ma</au><au>López-Arencibia, Atteneri</au><au>Sifaoui, Ines</au><au>Martínez-Carretero, Enrique</au><au>Valladares, Basilio</au><au>Piñero, José E.</au><au>Lorenzo-Morales, Jacob</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acanthamoeba genotypes T2, T4, and T11 in soil sources from El Hierro island, Canary Islands, Spain</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2016-08-01</date><risdate>2016</risdate><volume>115</volume><issue>8</issue><spage>2953</spage><epage>2956</epage><pages>2953-2956</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><abstract>The genus
Acanthamoeba
includes pathogenic strains which are causative agents of keratitis and encephalitis that often may end fatal in humans and other animals. In the present study, forty soil samples were collected in the island of El Hierro, Canary Islands, Spain, and checked for the presence of
Acanthamoeba
. Samples were cultivated onto 2 % non-nutrient agar plates seeded with a layer of heat killed
Escherichia coli
. Amplification by PCR and sequencing of the DF3 region of the 18S rDNA of
Acanthamoeba
was carried out in order to confirm morphological identification of the amoebae. Furthermore,
Acanthamoeba
spp. was isolated from 47.5 % of soil samples. Moreover, genotypes T2, T4, and T11 were identified in these samples. To the best of our knowledge, this is the first study to establish genotypes T2, T4, and T11 in soil sources from El Hierro island.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>27075307</pmid><doi>10.1007/s00436-016-5048-8</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0932-0113 |
| ispartof | Parasitology research (1987), 2016-08, Vol.115 (8), p.2953-2956 |
| issn | 0932-0113 1432-1955 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1868326523 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Acanthamoeba Acanthamoeba - genetics Acanthamoeba - isolation & purification Amoeba - genetics Animals Biomedical and Life Sciences Biomedicine DNA, Protozoan DNA, Ribosomal - genetics Escherichia coli Genetic aspects Genotype Genotypes Humans Immunology Medical Microbiology Microbiology Observations Original Paper Polymerase Chain Reaction Protozoans Soil - parasitology Spain |
| title | Acanthamoeba genotypes T2, T4, and T11 in soil sources from El Hierro island, Canary Islands, Spain |
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